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4. Comprehensive multivariate evaluation of the effects on cell phenotypes in multicolor flow cytometry data using ANOVA simultaneous component analysis

Author

Experimentele Afdeling Longziekten, CTI, Zorgeenheid Traumatologie, Infection & Immunity, Cancer, Bertinetto, Carlo G., Spijkerman, Roy, Hesselink, Lillian, Tinnevelt, Gerjen H., Bongers, Coen C.W.G., Postma, Geert J., Hopman, Maria T.E., Koenderman, Leo, Jansen, Jeroen J., Experimentele Afdeling Longziekten, CTI, Zorgeenheid Traumatologie, Infection & Immunity, Cancer, Bertinetto, Carlo G., Spijkerman, Roy, Hesselink, Lillian, Tinnevelt, Gerjen H., Bongers, Coen C.W.G., Postma, Geert J., Hopman, Maria T.E., Koenderman, Leo, and Jansen, Jeroen J.

Published
2023

7. Comprehensive multivariate evaluation of the effects on cell phenotypes in multicolor flow cytometry data using ANOVA simultaneous component analysis.

Author

Bertinetto, Carlo G., Spijkerman, Roy, Hesselink, Lillian, Tinnevelt, Gerjen H., Bongers, Coen C. W. G., Postma, Geert J., Hopman, Maria T. E., Koenderman, Leo, and Jansen, Jeroen J.

Subjects
FLOW cytometry, ANALYSIS of variance, STATISTICAL significance, PHENOTYPES, EXPERIMENTAL design
Abstract

This work proposes an approach to assess the effects observed in multicolor flow cytometry (MFC) experiments, for all markers and experimental factors simultaneously. It achieves this end by extending ANOVA simultaneous component analysis (ASCA), a multivariate version of ANOVA, to flow cytometry data. It is based on an initial multiset PCA model to describe the main variation patterns of cell marker expression, followed by an ASCA model on the histograms built from these PCA scores. This approach allows for determining the variations in cell phenotype distribution that are related to the experimental design. On a data set from a study of the immune response to prolonged physical exercise, the proposed method computed the effect size and statistical significance of all the experimental factors and their interactions. Most notably, it provided easily interpretable submodels for the overall effect of the walking exercise and for the interaction between exercise and the responsiveness to a bacterial stimulus. The application of a time‐guided sequential clustering algorithm to the ASCA scores revealed a stratification of the studied individuals based on their neutrophil activation dynamics. These effects were not clearly detectable using PCA alone. In comparison with pairwise classification models by DAMACY (a discriminant analysis method for MFC data), ASCA results were less detailed in describing differences between specific samples, but had the advantage of modeling several factors and levels simultaneously. Such characteristics make the proposed implementation of ASCA an effective and complementary addition to the chemometric methodologies for the analysis of MFC data. [ABSTRACT FROM AUTHOR]

Published
2023
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10. An increase in CD62L dim neutrophils precedes the development of pulmonary embolisms in COVID‐19 patients

Author

Spijkerman, Roy, Jorritsma, Nikita K. N., Bongers, Suzanne H., Bindels, Bas J. J., Jukema, Bernard N., Hesselink, Lillian, Hietbrink, Falco, Leenen, Luke P. H., Goor, Harriët M. R., Vrisekoop, Nienke, Kaasjager, Karin A. H., Koenderman, Leo, Stiphout, Feike, Nijdam, Thomas M. P., van de Ven, Nils L. M., Verhaegh, Remi, Spanjaard, Judith S., Verboeket, Benjamin W., Laane, Duco, van Wessem, Karlijn, van spengler, Daan E. J., Buitenwerf, Wiebe, Giustarini, Giulio, Mulder, Eva, Heijerman, Harry, Zabaleta, Amely Daza, van den bos, Frederique, Rademaker, Emma, Varkila, Meri R. J., de Mul, Nikki, Cremer, Olaf L., Slooter, Arjen, Delemarre, Eveline M., Nierkens, Stefan, Limper, Maarten, van Wijk, Femke, Pandit, Aridaman, Leavis, Helen, Clark, Chantal C., Barendrecht, Arjan D., Seinen, Cor W., Drost‐Verhoef, Sandra, Smits, Simone, Parr, Naomi M. J., Sebastian, Sylvie A. E., Koekman, Arnold C., van Wesel, Annet C., van der Vries, Erhard, Maas, Coen, de Maat, Steven, Haitjema, Saskia, and Hoefer, Imo E.

Subjects
Male, 0301 basic medicine, pulmonary embolism, Neutrophils, Receptor expression, SARS‐CoV‐2, Neutrophil Activation, law.invention, Cohort Studies, 0302 clinical medicine, Immunophenotyping, law, L‐selectin, L-Selectin, biology, Incidence (epidemiology), Regular Article, General Medicine, Middle Aged, Prognosis, Thrombosis, Intensive care unit, Pulmonary embolism, Intensive Care Units, Female, L-selectin, Disease Susceptibility, medicine.medical_specialty, Intensive Care Unit, Immunology, CD16, 03 medical and health sciences, COVID‐19, Internal medicine, medicine, CD62L, Humans, Aged, SARS-CoV-2, business.industry, COVID-19, medicine.disease, 030104 developmental biology, biology.protein, business, Biomarkers, Regular Articles, 030215 immunology
Abstract

Objectives A high incidence of pulmonary embolism (PE) is reported in patients with critical coronavirus disease 2019 (COVID‐19). Neutrophils may contribute to this through a process referred to as immunothrombosis. The aim of this study was to investigate the occurrence of neutrophil subpopulations in blood preceding the development of COVID‐19 associated PE. Methods We studied COVID‐19 patients admitted to the ICU of our tertiary hospital between 19‐03‐2020 and 17‐05‐2020. Point‐of‐care fully automated flow cytometry was performed prior to ICU admission, measuring the neutrophil activation/maturation markers CD10, CD11b, CD16 and CD62L. Neutrophil receptor expression was compared between patients who did or did not develop PE (as diagnosed on CT angiography) during or after their ICU stay. Results Among 25 eligible ICU patients, 22 subjects were included for analysis, of whom nine developed PE. The median (IQR) time between neutrophil phenotyping and PE occurrence was 9 (7‐12) days. A significant increase in the immune‐suppressive neutrophil phenotype CD16bright/CD62Ldim was observed on the day of ICU admission (P = 0.014) in patients developing PE compared to patients who did not. Conclusion The increase in this neutrophil phenotype indicates that the increased number of CD16bright/CD62Ldim neutrophils might be used as prognostic marker to predict those patients that will develop PE in critical COVID‐19 patients.

Published
2021

11. Refractory neutrophils and monocytes in patients with inflammatory bowel disease after repeated bouts of prolonged exercise

Author

Spijkerman, Roy, Hesselink, Lillian, Bertinetto, C.G., Bongers, C.C.W.G., Hietbrink, Falco, Vrisekoop, Nienke, Hopman, M.T.E., Jansen, J.J., Koenderman, Leo, Spijkerman, Roy, Hesselink, Lillian, Bertinetto, C.G., Bongers, C.C.W.G., Hietbrink, Falco, Vrisekoop, Nienke, Hopman, M.T.E., Jansen, J.J., and Koenderman, Leo

Abstract

Contains fulltext : 241528.pdf (Publisher’s version ) (Open Access)

Published
2021

12. Management of pediatric blunt abdominal trauma in a Dutch level one trauma center

Author

Zorgeenheid Traumatologie, Infection & Immunity, Spijkerman, Roy, Bulthuis, Lauren C.M., Hesselink, Lillian, Nijdam, Thomas M.P., Leenen, Luke P.H., de Bruin, Ivar G.J.M., Zorgeenheid Traumatologie, Infection & Immunity, Spijkerman, Roy, Bulthuis, Lauren C.M., Hesselink, Lillian, Nijdam, Thomas M.P., Leenen, Luke P.H., and de Bruin, Ivar G.J.M.

Published
2021

13. Refractory neutrophils and monocytes in patients with inflammatory bowel disease after repeated bouts of prolonged exercise

Author

Zorgeenheid Traumatologie, Infection & Immunity, Experimentele Afdeling Longziekten, Spijkerman, Roy, Hesselink, Lillian, Bertinetto, Carlo, Bongers, Coen C W G, Hietbrink, Falco, Vrisekoop, Nienke, Leenen, Luke P H, Hopman, Maria T E, Jansen, Jeroen J, Koenderman, Leo, Zorgeenheid Traumatologie, Infection & Immunity, Experimentele Afdeling Longziekten, Spijkerman, Roy, Hesselink, Lillian, Bertinetto, Carlo, Bongers, Coen C W G, Hietbrink, Falco, Vrisekoop, Nienke, Leenen, Luke P H, Hopman, Maria T E, Jansen, Jeroen J, and Koenderman, Leo

Published
2021

14. An increase in CD62Ldim neutrophils precedes the development of pulmonary embolisms in COVID-19 patients

Author

Experimentele Afdeling Longziekten, Cancer, Trialbureau Beeld, Zorgeenheid Traumatologie, MS Orthopaedie Algemeen, Infection & Immunity, Acute geneeskunde, MS Interne Geneeskunde, Circulatory Health, Medische Staf Intensive Care, Spijkerman, Roy, Jorritsma, Nikita Kn, Bongers, Suzanne H, Bindels, Bas Jj, Jukema, Bernard N, Hesselink, Lillian, Hietbrink, Falco, Leenen, Luke Ph, van Goor, Harriët Mr, Vrisekoop, N, Kaasjager, Karin Ah, Koenderman, Leo, COVPACH study group, Experimentele Afdeling Longziekten, Cancer, Trialbureau Beeld, Zorgeenheid Traumatologie, MS Orthopaedie Algemeen, Infection & Immunity, Acute geneeskunde, MS Interne Geneeskunde, Circulatory Health, Medische Staf Intensive Care, Spijkerman, Roy, Jorritsma, Nikita Kn, Bongers, Suzanne H, Bindels, Bas Jj, Jukema, Bernard N, Hesselink, Lillian, Hietbrink, Falco, Leenen, Luke Ph, van Goor, Harriët Mr, Vrisekoop, N, Kaasjager, Karin Ah, Koenderman, Leo, and COVPACH study group

Published
2021

15. Analysis of human neutrophil phenotypes as biomarker to monitor exercise-induced immune changes

Author

Experimentele Afdeling Longziekten, Zorgeenheid Traumatologie, Infection & Immunity, Spijkerman, Roy, Hesselink, Lillian, Bertinetto, Carlo, Bongers, Coen C.W.G., Hietbrink, Falco, Vrisekoop, Nienke, Leenen, Luke P.H., Hopman, Maria T.E., Jansen, Jeroen J., Koenderman, Leo, Experimentele Afdeling Longziekten, Zorgeenheid Traumatologie, Infection & Immunity, Spijkerman, Roy, Hesselink, Lillian, Bertinetto, Carlo, Bongers, Coen C.W.G., Hietbrink, Falco, Vrisekoop, Nienke, Leenen, Luke P.H., Hopman, Maria T.E., Jansen, Jeroen J., and Koenderman, Leo

Published
2021

17. Additional file 2 of New automated analysis to monitor neutrophil function point-of-care in the intensive care unit after trauma

Author

Hesselink, Lillian, Spijkerman, Roy, Fraiture, Emma De, Bongers, Suzanne, Wessem, Karlijn J. P. Van, Vrisekoop, Nienke, Koenderman, Leo, Leenen, Luke P. H., and Falco Hietbrink

Abstract

Additional file 2: Supplementary Figure 2. Gating strategy for distinguishing neutrophil subsets. The gating of the neutrophil subsets CD16dim/CD62Lbright, CD16bright/CD62Lbright and CD16bright/ CD62Ldim is shown.

Published
2020
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18. Additional file 5 of New automated analysis to monitor neutrophil function point-of-care in the intensive care unit after trauma

Author

Hesselink, Lillian, Spijkerman, Roy, Fraiture, Emma De, Bongers, Suzanne, Wessem, Karlijn J. P. Van, Vrisekoop, Nienke, Koenderman, Leo, Leenen, Luke P. H., and Falco Hietbrink

Abstract

Additional file 5: Supplementary Figure 4. Effect of temperature changes on neutrophil function. Neutrophil phagocytosis (a) and neutrophil phagosomal acidification (b) in five healthy controls at different temperatures. Blood from 5 healthy controls was analyzed after incubation for 60 minutes with double-labeled bioparticles on ice, in a water bath of 25°C and in a water bath of 37°C. Then, red blood cells were lysed using lysing reagent A and lysing reagent B from the AQUIOS CL® “Load & Go” flow cytometer and leukocyte analysis was performed using the BD FACSCanto™ II (BD Biosciences). Temperature conditions were compared using a Friedman test and a Mann-Whitney U Test with a Dunn's correction for multiple comparisons. Neutrophil phagocytosis and neutrophil acidification significantly differed at different temperatures (p = 0.0008 and p = 0.0394, respectively). Neutrophil phagocytosis increased as the temperature increased, and significant differences were found between samples that were kept on ice and samples that were kept in 37°C (p = 0.005). Such a temperature dependent trend was not observed for neutrophil acidification. However, significant differences were found between 25°C and 37°C (p = 0.034).

Published
2020
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19. Additional file 4 of New automated analysis to monitor neutrophil function point-of-care in the intensive care unit after trauma

Author

Hesselink, Lillian, Spijkerman, Roy, Fraiture, Emma De, Bongers, Suzanne, Wessem, Karlijn J. P. Van, Vrisekoop, Nienke, Koenderman, Leo, Leenen, Luke P. H., and Falco Hietbrink

Abstract

Additional file 4: Supplementary Figure 3. Neutrophil phagosomal acidification per neutrophil subset. Neutrophil phagosomal acidification of CD16dim/CD62Lbright cells (), CD16bright/CD62Lbright cells () and CD16bright/ CD62Ldim cells () in all patients after 10, 20, 40 and 60 minutes of incubation with S. Aureus bioparticles. Neutrophil phagosomal acidification after 60 minutes was compared between subsets using a one-way ANOVA. Significant differences were found between subsets (p < 0.001). A follow-up comparison of the means was performed with a Tukey’s correction for multiple comparisons. CD16dim/CD62Lbright neutrophils were found to acidify significantly better than CD16bright/CD62Lbright neutrophils (p = 0.016) and then CD16bright/CD62Lbright neutrophils (p < 0.001). MFI = median fluorescence intensity. PF520 = PromoFluor 520 LSS. S. Aureus = Staphylococcus Aureus. Data are presented as mean with standard error of the mean. *P

Published
2020
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20. Additional file 7 of New automated analysis to monitor neutrophil function point-of-care in the intensive care unit after trauma

Author

Hesselink, Lillian, Spijkerman, Roy, Fraiture, Emma De, Bongers, Suzanne, Wessem, Karlijn J. P. Van, Vrisekoop, Nienke, Koenderman, Leo, Leenen, Luke P. H., and Falco Hietbrink

Abstract

Additional file 7: Supplementary Figure 6. Bioparticles were internalized in neutrophils. ImageStream analysis showed that the bioparticles (green) were internalized in the neutrophil (staining of CD16 on cell membrane in blue). The experiment was performed using the Amnis® ImageStream®XMk II and data were analyzed using Exploration Software (IDEAS, Luminex, Austin, USA).

Published
2020
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21. Additional file 6 of New automated analysis to monitor neutrophil function point-of-care in the intensive care unit after trauma

Author

Hesselink, Lillian, Spijkerman, Roy, Fraiture, Emma De, Bongers, Suzanne, Wessem, Karlijn J. P. Van, Vrisekoop, Nienke, Koenderman, Leo, Leenen, Luke P. H., and Falco Hietbrink

Abstract

Additional file 6: Supplementary Figure 5. Effect of CD16/CD62L-antibodies on neutrophil function. Neutrophil phagocytosis (a) and neutrophil phagosomal acidification (b) in five healthy controls after 60 minutes of incubation with S. Aureus bioparticles. Per patient, this analysis was performed three times in different conditions: 1) with 6 μL Hepes buffer, 2) with 6 μL CD16-BV785 and 3) with 6 μL CD62L-BV650. The Hepes buffer and antibody-fluorochrome combinations were added to the wells plate prior to initiation of the functional analyses to prevent a time delay between the different analyses. The Hepes buffer consisted of 20 mM Hepes, 132 mM NaCl, 6 mM KCl, 1.2 mM KH2PO4 and 1 mM MgSO4, supplemented with 5 mM glucose, 1 mM CaCl2, and 0.5% (w/v) human serum albumin. The CD16/CD62L-antibody-fluorochrome combinations were chosen because the fluorochromes are not excited by the 488 laser of the AQUIOS CL® “Load & Go” flow cytometer. A Friedman test was used to compare the three different conditions. No significant differences in neutrophil phagocytosis and neutrophil phagosomal acidification were found after the addition of CD16 and CD62L antibodies. Data are presented as individual values with mean (black line). MFI = median fluorescence intensity. PF520 = PromoFluor 520 LSS. S. Aureus = Staphylococcus Aureus. Ns = non-significant.

Published
2020
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22. Additional file 1 of New automated analysis to monitor neutrophil function point-of-care in the intensive care unit after trauma

Author

Hesselink, Lillian, Spijkerman, Roy, Fraiture, Emma De, Bongers, Suzanne, Wessem, Karlijn J. P. Van, Vrisekoop, Nienke, Koenderman, Leo, Leenen, Luke P. H., and Falco Hietbrink

Abstract

Additional file 1: Supplementary Figure 1. Gating strategy for the determination of neutrophil phagocytosis and acidification. Granulocytes and lymphocytes can be distinguished on the forward scatter (FS)/side scatter (SS) (a). Neutrophils were identified by selecting granulocytes with CD16 expression (thereby excluding eosinophils) (b). Combined analysis of pHrodo® Green fluorescence and PF520 fluorescence allows for assessment of phagocytosis, expressed as a percentage of PF520-positive neutrophils (c), and neutrophil phagosomal acidification, expressed as the ratio pHrodo® Green fluorescence divided by PF520 fluorescence (d). PF520 = PromoFluor 520 LSS.

Published
2020
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24. Persistent inflammation, immunosuppression and catabolism syndrome (PICS) after polytrauma: A rare syndrome with major consequences

Author

Zorgeenheid Traumatologie, Experimentele Afdeling Longziekten, Other research (not in main researchprogram), CDL Arcadia, Infection & Immunity, Hesselink, Lillian, Hoepelman, Ruben J., Spijkerman, Roy, de Groot, Mark C.H., van Wessem, Karlijn J.P., Koenderman, Leo, Leenen, Luke P.H., Hietbrink, Falco, Zorgeenheid Traumatologie, Experimentele Afdeling Longziekten, Other research (not in main researchprogram), CDL Arcadia, Infection & Immunity, Hesselink, Lillian, Hoepelman, Ruben J., Spijkerman, Roy, de Groot, Mark C.H., van Wessem, Karlijn J.P., Koenderman, Leo, Leenen, Luke P.H., and Hietbrink, Falco

Published
2020

25. Fragile neutrophils in surgical patients: A phenomenon associated with critical illness

Author

Zorgeenheid Traumatologie, Other research (not in main researchprogram), CDL Arcadia, CDL Staf Patiëntenzorg KC, Centraal Diagnostisch Laboratorium, Infection & Immunity, Experimentele Afdeling Longziekten, Hesselink, Lillian, Spijkerman, Roy, Hellebrekers, Pien, van Bourgondiën, Robert J, Blasse, Enja, Haitjema, Saskia, Huisman, Albert, van Solinge, Wouter W, Van Wessem, Karlijn J P, Koenderman, Leo, Leenen, Luke P H, Hietbrink, Falco, Zorgeenheid Traumatologie, Other research (not in main researchprogram), CDL Arcadia, CDL Staf Patiëntenzorg KC, Centraal Diagnostisch Laboratorium, Infection & Immunity, Experimentele Afdeling Longziekten, Hesselink, Lillian, Spijkerman, Roy, Hellebrekers, Pien, van Bourgondiën, Robert J, Blasse, Enja, Haitjema, Saskia, Huisman, Albert, van Solinge, Wouter W, Van Wessem, Karlijn J P, Koenderman, Leo, Leenen, Luke P H, and Hietbrink, Falco

Published
2020

26. Point-of-Care Analysis of Neutrophil Phenotypes: A First Step Toward Immuno-Based Precision Medicine in the Trauma ICU

Author

Zorgeenheid Traumatologie, Infection & Immunity, Experimentele Afdeling Longziekten, Spijkerman, Roy, Hesselink, Lillian, Bongers, Suzanne, van Wessem, Karlijn J P, Vrisekoop, Nienke, Hietbrink, Falco, Koenderman, Leo, Leenen, Luke P H, Zorgeenheid Traumatologie, Infection & Immunity, Experimentele Afdeling Longziekten, Spijkerman, Roy, Hesselink, Lillian, Bongers, Suzanne, van Wessem, Karlijn J P, Vrisekoop, Nienke, Hietbrink, Falco, Koenderman, Leo, and Leenen, Luke P H

Published
2020

27. New automated analysis to monitor neutrophil function point-of-care in the intensive care unit after trauma

Author

Zorgeenheid Traumatologie, Experimentele Afdeling Longziekten, Infection & Immunity, Hesselink, Lillian, Spijkerman, Roy, de Fraiture, Emma, Bongers, Suzanne, Van Wessem, Karlijn J P, Vrisekoop, Nienke, Koenderman, Leo, Leenen, Luke P H, Hietbrink, Falco, Zorgeenheid Traumatologie, Experimentele Afdeling Longziekten, Infection & Immunity, Hesselink, Lillian, Spijkerman, Roy, de Fraiture, Emma, Bongers, Suzanne, Van Wessem, Karlijn J P, Vrisekoop, Nienke, Koenderman, Leo, Leenen, Luke P H, and Hietbrink, Falco

Published
2020

29. Fragile neutrophils in surgical patients: A phenomenon associated with critical illness

Author

Hesselink, Lillian, primary, Spijkerman, Roy, additional, Hellebrekers, Pien, additional, van Bourgondiën, Robert J., additional, Blasse, Enja, additional, Haitjema, Saskia, additional, Huisman, Albert, additional, van Solinge, Wouter W., additional, Van Wessem, Karlijn J. P., additional, Koenderman, Leo, additional, Leenen, Luke P. H., additional, and Hietbrink, Falco, additional

Published
2020
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34. Automated flow cytometry enables high performance point-of-care analysis of leukocyte phenotypes

Author

Experimentele Afdeling Longziekten, Zorgeenheid Traumatologie, Other research (not in main researchprogram), Infection & Immunity, Spijkerman, Roy, Hesselink, Lillian, Hellebrekers, Pien, Vrisekoop, Nienke, Hietbrink, Falco, Leenen, Luke P H, Koenderman, Leo, Experimentele Afdeling Longziekten, Zorgeenheid Traumatologie, Other research (not in main researchprogram), Infection & Immunity, Spijkerman, Roy, Hesselink, Lillian, Hellebrekers, Pien, Vrisekoop, Nienke, Hietbrink, Falco, Leenen, Luke P H, and Koenderman, Leo

Published
2019

35. Neutrophil heterogeneity and its role in infectious complications after severe trauma

Author

Zorgeenheid Traumatologie, Infection & Immunity, Experimentele Afdeling Longziekten, Hesselink, Lillian, Spijkerman, Roy, van Wessem, Karlijn J P, Koenderman, Leo, Leenen, Luke P H, Huber-Lang, Markus, Hietbrink, Falco, Zorgeenheid Traumatologie, Infection & Immunity, Experimentele Afdeling Longziekten, Hesselink, Lillian, Spijkerman, Roy, van Wessem, Karlijn J P, Koenderman, Leo, Leenen, Luke P H, Huber-Lang, Markus, and Hietbrink, Falco

Published
2019

36. A Rise in Neutrophil Cell Size Precedes Organ Dysfunction After Trauma

Author

Zorgeenheid Traumatologie, CDL Staf Patiëntenzorg KC, Infection & Immunity, Other research (not in main researchprogram), CDL Arcadia, Circulatory Health, Centraal Diagnostisch Laboratorium, Experimentele Afdeling Longziekten, Hesselink, Lillian, Heeres, Marjolein, Paraschiakos, Fotis, Ten Berg, Maarten, Huisman, Albert, Hoefer, Imo E., de Groot, Mark C.H., van Solinge, Wouter W., Dijkgraaf, Marcel, Hellebrekers, Pien, Van Wessem, Karlijn J.P., Koenderman, Leo, Leenen, Luke P.H., Hietbrink, Falco, Zorgeenheid Traumatologie, CDL Staf Patiëntenzorg KC, Infection & Immunity, Other research (not in main researchprogram), CDL Arcadia, Circulatory Health, Centraal Diagnostisch Laboratorium, Experimentele Afdeling Longziekten, Hesselink, Lillian, Heeres, Marjolein, Paraschiakos, Fotis, Ten Berg, Maarten, Huisman, Albert, Hoefer, Imo E., de Groot, Mark C.H., van Solinge, Wouter W., Dijkgraaf, Marcel, Hellebrekers, Pien, Van Wessem, Karlijn J.P., Koenderman, Leo, Leenen, Luke P.H., and Hietbrink, Falco

Published
2019

37. An increase in CD62Ldim neutrophils precedes the development of pulmonary embolisms in COVID‐19 patients.

Author

Spijkerman, Roy, Jorritsma, Nikita K. N., Bongers, Suzanne H., Bindels, Bas J. J., Jukema, Bernard N., Hesselink, Lillian, Hietbrink, Falco, Leenen, Luke P. H., Goor, Harriët M. R., Vrisekoop, Nienke, Kaasjager, Karin A. H., Koenderman, Leo, Stiphout, Feike, Nijdam, Thomas M. P., van de Ven, Nils L. M., Verhaegh, Remi, Spanjaard, Judith S., Verboeket, Benjamin W., Laane, Duco, and van Wessem, Karlijn

Subjects
COVID-19, PULMONARY embolism, NEUTROPHILS
Abstract

Objectives: A high incidence of pulmonary embolism (PE) is reported in patients with critical coronavirus disease 2019 (COVID‐19). Neutrophils may contribute to this through a process referred to as immunothrombosis. The aim of this study was to investigate the occurrence of neutrophil subpopulations in blood preceding the development of COVID‐19 associated PE. Methods: We studied COVID‐19 patients admitted to the ICU of our tertiary hospital between 19‐03‐2020 and 17‐05‐2020. Point‐of‐care fully automated flow cytometry was performed prior to ICU admission, measuring the neutrophil activation/maturation markers CD10, CD11b, CD16 and CD62L. Neutrophil receptor expression was compared between patients who did or did not develop PE (as diagnosed on CT angiography) during or after their ICU stay. Results: Among 25 eligible ICU patients, 22 subjects were included for analysis, of whom nine developed PE. The median (IQR) time between neutrophil phenotyping and PE occurrence was 9 (7‐12) days. A significant increase in the immune‐suppressive neutrophil phenotype CD16bright/CD62Ldim was observed on the day of ICU admission (P = 0.014) in patients developing PE compared to patients who did not. Conclusion: The increase in this neutrophil phenotype indicates that the increased number of CD16bright/CD62Ldim neutrophils might be used as prognostic marker to predict those patients that will develop PE in critical COVID‐19 patients. [ABSTRACT FROM AUTHOR]

Published
2021
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38. Analysis of human neutrophil phenotypes as biomarker to monitor exercise‐induced immune changes.

Author

Spijkerman, Roy, Hesselink, Lillian, Bertinetto, Carlo, Bongers, Coen CWG, Hietbrink, Falco, Vrisekoop, Nienke, Leenen, Luke PH, Hopman, Maria TE, Jansen, Jeroen J, and Koenderman, Leo

Subjects
HUMAN phenotype, PSYCHONEUROIMMUNOLOGY, IMMUNE response, IMMUNE system, EXERCISE intensity, PHYSIOLOGICAL stress
Abstract

The amplitude of the innate immune response reflects the degree of physiological stress imposed by exercise load. An optimal balance of exercise intensity and duration is essential for a balanced immune system and reduces the risk of dysfunction of the immune system. Therefore, it is hypothesized that neutrophils, as key players in the innate immune system, can be used as biomarker in detecting overtraining. The aim was to monitor the state of the innate immune system by phenotyping neutrophils during consecutive bouts of prolonged exercise. Study subjects were recruited from a cohort of walkers participating in a walking event on 3 consecutive days. Participants with immune deficiencies were excluded. Questionnaires to determine the physiological status of the participants were completed. Analysis of neutrophil receptor expression was done by a point‐of‐care fully automated flow cytometer. A total of 45 participants were recruited, of whom 39 participants were included for data analysis. Study participants had a median age of 64 (58‐70) years. The absolute numbers CD16dim/CD62Lbright and CD16bright/CD62Ldim neutrophils were increased after the first 2 days of exercise followed by an adaptation/normalization after the third day. Participants with activated neutrophils (high CD11b expression) had an impaired physical feeling indicated by the participant on a lower visual analog scale compared to participants who did not have activated neutrophils (P = 0.017, P = 0.022). Consecutive days of prolonged exercise results in an initial systemic innate immune response, followed by normalization/adaptation. Increased neutrophil activation was associated with impaired physical feeling measured by a validated VAS score indicated by the participant. Fully automated point‐of‐care flow cytometry analysis of neutrophil phenotypes in a field laboratory might be a useful tool to monitor relevant differences in the systemic innate immune response in response to exercise. [ABSTRACT FROM AUTHOR]

Published
2021
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39. An increase in myeloid cells after severe injury is associated with normal fracture healing : a retrospective study of 62 patients with a femoral fracture

Author

Hesselink, Lillian, Bastian, Okan W, Heeres, Marjolein, Ten Berg, Maarten, Huisman, Albert, Hoefer, Imo E, van Solinge, Wouter W, Koenderman, Leo, van Wessem, Karlijn J P, Leenen, Luke P H, Hietbrink, Falco, Hesselink, Lillian, Bastian, Okan W, Heeres, Marjolein, Ten Berg, Maarten, Huisman, Albert, Hoefer, Imo E, van Solinge, Wouter W, Koenderman, Leo, van Wessem, Karlijn J P, Leenen, Luke P H, and Hietbrink, Falco

Published
2018

40. An increase in myeloid cells after severe injury is associated with normal fracture healing: a retrospective study of 62 patients with a femoral fracture

Author

Zorgeenheid Traumatologie, Heelkunde Opleiding, CDL Staf Patiëntenzorg KC, Infection & Immunity, Other research (not in main researchprogram), CDL Arcadia, Circulatory Health, Centraal Diagnostisch Laboratorium, Experimentele Afdeling Longziekten, Hesselink, Lillian, Bastian, Okan W, Heeres, Marjolein, Ten Berg, Maarten, Huisman, Albert, Hoefer, Imo E, van Solinge, Wouter W, Koenderman, Leo, van Wessem, Karlijn J P, Leenen, Luke P H, Hietbrink, Falco, Zorgeenheid Traumatologie, Heelkunde Opleiding, CDL Staf Patiëntenzorg KC, Infection & Immunity, Other research (not in main researchprogram), CDL Arcadia, Circulatory Health, Centraal Diagnostisch Laboratorium, Experimentele Afdeling Longziekten, Hesselink, Lillian, Bastian, Okan W, Heeres, Marjolein, Ten Berg, Maarten, Huisman, Albert, Hoefer, Imo E, van Solinge, Wouter W, Koenderman, Leo, van Wessem, Karlijn J P, Leenen, Luke P H, and Hietbrink, Falco

Published
2018

41. An increase in myeloid cells after severe injury is associated with normal fracture healing: a retrospective study of 62 patients with a femoral fracture

Author

Hesselink, Lillian, primary, Bastian, Okan W, additional, Heeres, Marjolein, additional, ten Berg, Maarten, additional, Huisman, Albert, additional, Hoefer, Imo E, additional, van Solinge, Wouter W, additional, Koenderman, Leo, additional, van Wessem, Karlijn J P, additional, Leenen, Luke P H, additional, and Hietbrink, Falco, additional

Published
2018
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