Search

Your search keyword '"Hertel, Robert"' showing total 196 results
Start Over Author "Hertel, Robert"
196 results on '"Hertel, Robert"'

12. The low mutational flexibility of the EPSP synthase in Bacillus subtilis is due to a higher demand for shikimate pathway intermediates

Author

Schwedt, Inge, primary, Schöne, Kerstin, additional, Eckert, Maike, additional, Pizzinato, Manon, additional, Winkler, Laura, additional, Knotkova, Barbora, additional, Richts, Björn, additional, Hau, Jann‐Louis, additional, Steuber, Julia, additional, Mireles, Raul, additional, Noda‐Garcia, Lianet, additional, Fritz, Günter, additional, Mittelstädt, Carolin, additional, Hertel, Robert, additional, and Commichau, Fabian M., additional

Published
2023
Full Text
View/download PDF

18. Vibrio syngnathi sp. nov., a fish pathogen, isolated from the Kiel Fjord

Author

Chibani, Cynthia Maria, primary, Hertel, Robert, additional, Neumann-Schaal, Meina, additional, Goehlich, Henry, additional, Wagner, Kim, additional, Bunk, Boyke, additional, Spröer, Cathrin, additional, Overmann, Jörg, additional, Hoppert, Michael, additional, Marten, Silke Mareike, additional, Roth, Olivia, additional, Liesegang, Heiko, additional, and Wendling, Carolin C., additional

Published
2023
Full Text
View/download PDF

20. COMPLETE GENOME SEQUENCE OF VIBRIO SYNGNATHI SP. NOV., A FISH PATHOGEN, ISOLATED FROM THE KIEL FJORD

Author

Chibani, Cynthia M, primary, Hertel, Robert, additional, Neumann-Schaal, Meina, additional, Goehlich, Henry, additional, Wagner, Kim, additional, Bunk, Boyke, additional, Sproeer, Cathrin, additional, Overmann, Joerg, additional, Hoppert, Michael, additional, Marten, Mareike, additional, Roth, Olivia, additional, Liesegang, Heiko, additional, and Wendling, Carolin Charlotte, additional

Published
2023
Full Text
View/download PDF

21. Isolation of a host-confined phage metagenome allows the detection of phages both capable and incapable of plaque formation

Author

Streit, Wolfgang R., Daniel, Rolf, Friedrich, Ines, Hertel, Robert, Streit, Wolfgang R., Daniel, Rolf, Friedrich, Ines, and Hertel, Robert

Abstract

Bacteriophages, also called phages, are viruses of bacteria. They are the most common and diverse biological entities on this planet. For metagenomic investigation, their diversity is also their biggest obstacle. The direct metagenomic sequence of environmental phage communities often leads to short genomic fragments limiting the investigation to a few individual aspects of phage biology and diversity. The presented protocol for generating a host-associated metagenome reduces the phage diversity to a concise and accessible size. Metagenome sequencing often leads to complete genomes, and the availability of a suitable host system ensures further experimental investigation.

Published
2023
Full Text
View/download PDF

22. Vibrio syngnathi sp. nov., a fish pathogen, isolated from the Kiel Fjord

Author

Chibani, Cynthia Maria, Hertel, Robert, Neumann-Schaal, Meina, Goehlich, Henry, Wagner, Kim-Sara, Bunk, Boyke, Spröer, Cathrin, Overmann, Jörg, Hoppert, Michael, Marten, Silke-Mareike, Roth, Olivia, Liesegang, Heiko, Wendling, Carolin C., Chibani, Cynthia Maria, Hertel, Robert, Neumann-Schaal, Meina, Goehlich, Henry, Wagner, Kim-Sara, Bunk, Boyke, Spröer, Cathrin, Overmann, Jörg, Hoppert, Michael, Marten, Silke-Mareike, Roth, Olivia, Liesegang, Heiko, and Wendling, Carolin C.

Published
2023
Full Text
View/download PDF

23. CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis

Author

Streit, Wolfgang R., Daniel, Rolf, Kohm, Katharina, Lutz, Veronika Theresa, Friedrich, Ines, Hertel, Robert, Streit, Wolfgang R., Daniel, Rolf, Kohm, Katharina, Lutz, Veronika Theresa, Friedrich, Ines, and Hertel, Robert

Abstract

Phages are viruses of bacteria and have been known for over a century. They do not have a metabolism or protein synthesis machinery and rely on host cells for replication. The model organism Bacillus subtilis has served as a host strain for decades and enabled the isolation of many unique viral strains. However, many viral species representatives remained orphans as no, or only a few, related phages were ever re-isolated. The presented protocol describes how a CRISPR-Cas9 system with an artificial CRISPR-array can be set up and used to discriminate abundant and well-known B. subtilis phage from a host-based metagenome enrichment. The obtained viral suspension can be used for metagenome sequencing and isolating new viral strains.

Published
2023

24. Deep Learning Techniques for COVID-19 Diagnosis and Prognosis Based on Radiological Imaging.

Author

HERTEL, ROBERT and BENLAMRI, RACHID

Subjects
*DEEP learning, *COVID-19 pandemic, *COVID-19 testing, *MEDICAL personnel, *COVID-19, *PROGNOSIS
Abstract

This literature review summarizes the current deep learning methods developed by the medical imaging AI research community that have been focused on resolving lung imaging problems related to coronavirus disease 2019 (COVID-19). COVID-19 shares many of the same imaging characteristics as other common forms of bacterial and viral pneumonia. Differentiating COVID-19 from other common pulmonary infections is a non-trivial task. To help offset what commonly requires hours of tedious manual annotation, several innovative solutions have been published to help healthcare providers during the COVID-19 pandemic. However, the absence of a comprehensive survey on the subject makes it challenging to ascertain which approaches are promising and therefore deserve further investigation. In this survey, we present an in-depth review of deep learning techniques that have recently been applied to the task of discovering the diagnosis and prognosis of COVID-19 patients. We categorize existing approaches based on features such as dimensionality of radiological imaging, system purpose, and used deep learning techniques, underlying core issues, and challenges. We also address the merits and shortcomings of various approaches, and finally we discuss future directions for this research. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

25. Brevundimonas and Serratia as host systems for assessing associated environmental viromes and phage diversity by complementary approaches

Author

Friedrich, Ines, primary, Neubauer, Hannes, additional, Kuritsyn, Alisa, additional, Bodenberger, Bernhard, additional, Tskhay, Faina, additional, Hartmann, Sara, additional, Poehlein, Anja, additional, Bömeke, Mechthild, additional, Hoppert, Michael, additional, Schneider, Dominik, additional, Hertel, Robert, additional, and Daniel, Rolf, additional

Published
2023
Full Text
View/download PDF

30. The Bacillus phage SPβ and its relatives

Author

Kohm, Katharina, Floccari, Valentina Andrea, Lutz, Veronika T., Nordmann, Birthe, Mittelstädt, Carolin, Poehlein, Anja, Dragoš, Anna, Commichau, Fabian M., and Hertel, Robert

Subjects
profagi, udc:579.852.11:578.347:575.111, genomika, lizogenija, divji tip sevov, Bacillus subtilis
Abstract

The Bacillus phage SPβ has been known for about 50 years, but only a few strains are available. We isolated four new wild-type strains of the SPbeta species. Phage vB_BsuS-Goe14 introduces its prophage into the spoVK locus, previously not observed to be used by SPβ-like phages. Sequence data revealed the genome replication strategy and the genome packaging mode of SPβ-like phages. We extracted 55 SPβ-like prophages from public Bacillus genomes, thereby discovering three more integration loci and one additional type of integrase. The identified prophages resemble four new species clusters and three species orphans in the genus Spbetavirus. The determined core proteome of all SPβ-like prophages consists of 38 proteins. The integration cassette proved to be not conserved, even though, present in all strains. It consists of distinct integrases. Analysis of SPβ transcriptomes revealed three conserved genes, yopQ, yopR, and yokI, to be transcribed from a dormant prophage. While yopQ and yokI could be deleted from the prophage without activating the prophage, damaging of yopR led to a clear-plaque phenotype. Under the applied laboratory conditions, the yokI mutant showed an elevated virion release implying the YokI protein being a component of the arbitrium system.

Published
2022

31. The Bacillus phage SPβ and its relatives: A temperate phage model system reveals new strains, species, prophage integration loci, conserved proteins and lysogeny management components

Author

Kohm, Katharina, primary, Floccari, Valentina A., additional, Lutz, Veronika T., additional, Nordmann, Birthe, additional, Mittelstaedt, Carolin, additional, Poehlein, Anja, additional, Dragos, Anna, additional, Commichau, Fabian M., additional, and Hertel, Robert, additional

Published
2021
Full Text
View/download PDF

32. Characterization of glyphosate‐resistant Burkholderia anthina and Burkholderia cenocepacia isolates from a commercial Roundup® solution

Author

Hertel, Robert, primary, Schöne, Kerstin, additional, Mittelstädt, Carolin, additional, Meißner, Janek, additional, Zschoche, Nick, additional, Collignon, Madeline, additional, Kohler, Christian, additional, Friedrich, Ines, additional, Schneider, Dominik, additional, Hoppert, Michael, additional, Kuhn, Ramona, additional, Schwedt, Inge, additional, Scholz, Patricia, additional, Poehlein, Anja, additional, Martienssen, Marion, additional, Ischebeck, Till, additional, Daniel, Rolf, additional, and Commichau, Fabian M., additional

Published
2021
Full Text
View/download PDF

37. First Complete Genome Sequences of Janthinobacterium lividum EIF1 and EIF2 and their Comparative Genome Analysis

Author

Friedrich, Ines, Hollensteiner, Jacqueline, Schneider, Dominik, Poehlein, Anja, Hertel, Robert, and Daniel, Rolf

Subjects
Janthinobacterium lividum, secondary metabolites, violacein operon, antimicrobial, antifungal, xenoamicin
Abstract

We present the first two complete genomes of the Janthinobacterium lividum species, namely strains EIF1 and EIF2, which both possess the ability to synthesize violacein. The violet pigment violacein is a secondary metabolite with antibacterial, antifungal, antiviral, and antitumoral properties. Both strains were isolated from environmental oligotrophic water ponds in Göttingen. The strains were phylogenetically classified by ANI analysis and showed a species assignment to Janthinobacterium lividum with 97.72% (EIF1) and 97.66% (EIF2) identity. These are the first complete genome sequences of strains belonging to the species Janthinobacterium lividum. The genome of strain EIF1 consists of one circular chromosome (6,373,589 bp) with a GC-content of 61.98%. The genome contains 5,551 coding sequences, 122 rRNAs, 93 tRNAs, and 1 tm-RNA. The genome of EIF2 comprises one circular chromosome (6,399,352 bp) with a GC-content of 61.63% and a circular plasmid p356839 (356,839 bp) with a GC-content of 57.21%. The chromosome encodes 5,691 coding sequences, 122 rRNAs, 93 tRNAs, and 1 tm-RNA and the plasmid harbors 245 coding sequences. In addition to the highly conserved chromosomally encoded violacein operon, the plasmid comprises a non-ribosomal peptide synthetase cluster (NRPS) with similarity to xenoamicin, which is a bioactive compound effective against protozoan parasites. Open-Access-Publikationsfonds 2020 peerReviewed

Published
2020

47. Characterization of glyphosate‐resistant Burkholderia anthina and Burkholderia cenocepacia isolates from a commercial Roundup® solution.

Author

Hertel, Robert, Schöne, Kerstin, Mittelstädt, Carolin, Meißner, Janek, Zschoche, Nick, Collignon, Madeline, Kohler, Christian, Friedrich, Ines, Schneider, Dominik, Hoppert, Michael, Kuhn, Ramona, Schwedt, Inge, Scholz, Patricia, Poehlein, Anja, Martienssen, Marion, Ischebeck, Till, Daniel, Rolf, and Commichau, Fabian M.

Subjects
*GLYPHOSATE, *BURKHOLDERIA cenocepacia, *BURKHOLDERIA, *MICROBIAL sensitivity tests, *HERBICIDES, *DRUG resistance in bacteria, *SOIL microbiology
Abstract

Summary: Roundup® is the brand name for herbicide solutions containing glyphosate, which specifically inhibits the 5‐enolpyruvyl‐shikimate‐3‐phosphate (EPSP) synthase of the shikimate pathway. The inhibition of the EPSP synthase causes plant death because EPSP is required for biosynthesis of aromatic amino acids. Glyphosate also inhibits the growth of archaea, bacteria, Apicomplexa, algae and fungi possessing an EPSP synthase. Here, we have characterized two glyphosate‐resistant bacteria from a Roundup solution. Taxonomic classification revealed that the isolates 1CH1 and 2CH1 are Burkholderia anthina and Burkholderia cenocepacia strains respectively. Both isolates cannot utilize glyphosate as a source of phosphorus and synthesize glyphosate‐sensitive EPSP synthase variants. Burkholderia. anthina 1CH1 and B. cenocepacia 2CH1 tolerate high levels of glyphosate because the herbicide is not taken up by the bacteria. Previously, it has been observed that the exposure of soil bacteria to herbicides like glyphosate promotes the development of antibiotic resistances. Antibiotic sensitivity testing revealed that the only the B. cenocepacia 2CH1 isolate showed increased resistance to a variety of antibiotics. Thus, the adaptation of B. anthina 1CH1 and B. cenocepacia 2CH1 to glyphosate did not generally increase the antibiotic resistance of both bacteria. However, our study confirms the genomic adaptability of bacteria belonging to the genus Burkholderia. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results