1. Autoradiographic assessment of 3H-proline uptake by osteoblasts following guanethidine-induced sympathectomy in the rat
- Author
-
Herskovits Ms, Robert M. Klein, and I. J. Singh
- Subjects
Guanethidine ,medicine.medical_specialty ,Histology ,Sympathetic Nervous System ,Proline ,medicine.medical_treatment ,GUANETHIDINE SULFATE ,Bone and Bones ,Pathology and Forensic Medicine ,Osteogenesis ,Internal medicine ,medicine ,Animals ,3h proline ,Osteoblasts ,Chemistry ,Cell Biology ,Molecular medicine ,Rats ,Endocrinology ,Sympathectomy ,Femoral diaphysis ,Celiac ganglion ,Autoradiography ,medicine.drug - Abstract
Sympathectomy was carried out in rats by injections of guanethidine-sulfate from birth to 14 days of age. At 45 days of age, the activity of osteoblastic cells was monitored by 3H-proline autoradiography. Effectiveness of sympathectomy was verified by light-microscopic examination of superior cervical and celiac ganglia. Grain counts over periosteal osteoblasts of the femoral diaphysis and osteoblasts mesial to the first molar in the mandible demonstrated a significantly reduced uptake of 3H-proline in the sympathectomized rats. The data provide direct evidence of sympathetic influence on osteoblastic activity and suggest that sympathectomy may result in the loss of a trophic influence which is important in the regulation of osteogenesis.
- Published
- 1981