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Phlebopus portentosus (Berk. & Broome) Boedijn was firstly constructed from the collection of Indonesia in 1951. To date, the subsequent collection of this ectomycorrhizal (ECM) fungi has not been done in Indonesia. In addition, the utilization information of this edible mushroom as food is not popular for the country. The goal of our work was to update the current collection, provide the morphological and molecular data, and promote the utilization of this edible mushroom in Indonesia. Fresh fruiting bodies were evaluated for morphological and molecular evidence. The basidiomata were analysis on the basis of the morphological and molecular evidence. The phylogenetic tree was constructed following the rDNA-ITS 1/2 sequence. P. portentosus was verified by morphological and phylogenetic studies combined. The indigenous people in the research site use this wild edible mushroom for self-consumption and sell it to local market. Phlebopus portentosus BO24626 was solitary to connate, boletoid basidioma, yellowish to greenish brown pileus and stipe, yellowish hymenophore, clavate stipe, club shaped basidia, oval to subglobose basidiospores, cystidia present. The absence of sponge-like tissues and the occurrence of sterigmata distinguished our specimens from the similar morphological species P. spongious. The lack of reddish stipe distinguished our specimen from P. roseus. In addition, the presence of hymenial cystidia delimits our specimens from P. colossus. The inferred phylogenetic tree nested our specimen in the group of P. portentosus (sister to P. spongiosus). The ITS sequence of our specimen is now deposited at GenBank and can be applied to upcoming research of P. portentosus. [ABSTRACT FROM AUTHOR]

The genus Pleurotus is known as a commercially important mushroom and one of the most well-known cultivated mushrooms worldwide. Of many species of Pleurotus, the phoenix mushroom (P. pulmonarius) is cultivated in many countries, including Indonesia. In Indonesia, the farmers and larger companies usually use commercial strains of phoenix mushroom which they purchased from other countries. To date, there was no prior information regarding wild occurrences of P. pulmonarius in Indonesia. During our regular mushroom hunting in Sukabumi, West Java, Indonesia, some edible wild fruiting bodies of light brown Pleurotus were collected. The current study aimed to determine the taxonomical position of our specimens based on morphological and molecular evidence. The combination of morphological and molecular analysis confirmed our specimens as P. pulnonarius. Morphologically, our specimens were distinguished by the small to medium sized fruiting bodies, pileus light brown, pinkish brown, to pale brown, flabelliform in the beginning to expanding broadly ovoid in maturity, lamellae shortly to deeply decurrent, stipe fleshy, eccentric to lateral, concolorous with lamellae, Basidiospores cylindrical to ellipsoid, basidia clavate to club shaped, basidioles are abundant, oleiferous hyphae common. The BLAST result revealed that our specimens posed a high similarity to P. pulnonarius from several countries as the top hits. The ITS phylogenetic tree placed Pleurotus FIPIA-DEP51 in the same clade of P. pulnonarius with 100% BS value. This study reports for the first time the wild occurences of P. pulmonarius in Indonesia. Future study should be done to characterize the cultures of reported mushroom which can potentially be the local strain for cultivation of P. pulmonarius industry in Indonesia. [ABSTRACT FROM AUTHOR]

Pegler suggested that shiitake comprises three morphological species: Lentinula edodes (continental and northeast Asia), L. lateritia (tropical Asia and Australasia), and L. novae-zelandiae (New Zealand). The current study reported for the first time the occurrence of L. lateritia (Berk.) Pegler in Indonesia. During a fungus foray in Kerinci (Jambi, Sumatra, Indonesia) in 2022 and 2023 by the Indonesian mushroom hunter community, some basidiomata of Lentinula were obtained. At a glance, our specimens resembled L. edodes. The current study aims to justify the taxonomical position of our specimens based on morphological and molecular data. The fresh basidiomata were used for morphological and molecular analyses. The molecular work was done using ITS 4/5 Primers for phylogenetic analysis of rDNA-ITS region. Morphologically, the uniformly reddish brown, smooth, and glabrous of pileus confirmed our specimens as L. lateritia. In addition, the absence of a range of colors and squamules pileus distinguished L. lateritia BO24628 form L. edodes, while the formation of florets cheilocystidia in L. madagasikarensis was the distinctive character of our specimens. The BLAST result revealed that our specimen has high similarity (99-100%) with L. lateritia and L. edodes as the top hits. The phylogenetic tree (RAxML) nested our specimens in the L. lateria clade and is closely related to one specimen from Papua New Guenia (PNG) (BS 98%). In addition, L. lateritia BO24628 has a sister clade of the specimen from PNG and Australia. Moreover, we provide the herbarium collection of wild L. lateritia in Indonesia. [ABSTRACT FROM AUTHOR]

Panus lecomtei (Polyporales) is reported for the first time from Indonesia at the IPB University Campus Forest (IPBUCF). This species has similar macroscopic features to P. strigellus and P. neostrigosus. This study aimed to confirm the taxonomic position of P. lecomtei found at IPBUCF based on morphological and molecular evidence. The specimens were described by their macroscopic and microscopic characteristics. A phylogenetic approach was used with the internal transcribed spacer 1/2 sequence data. The combination of morphological and molecular analyses confirmed the taxonomic position of our specimens as P. lecomtei. The recorded species was distinguished by gregarious basidiomata, an infundibuliform resembling a funnel, violet during the immature stage to orangish brown in the mature pileus and ellipsoid basidiospore stages. Our specimens were distinguished from P. strigellus by having smaller basidiospores. It was difficult to separate P. lecomtei BO24428 from P. neostrigosus based on morphological characters. However, the BLAST results showed that specimen BO24428 had 99.56% similarity with P. lecomtei. In addition, our phylogenetic tree nested P. lecomtei BO24428 in the P. lecomtei clade. Morphological illustrations and a phylogenetic tree of P. lecomtei are provided. We suggest combining the morphological and molecular evidence in future studies of P. lecomtei from Indonesia. [ABSTRACT FROM AUTHOR]

Podostroma cornu-damae is considered one of the most poisonous mushrooms in the world. The first report of P. cornudamae in Indonesia was provided by Boedijn in 1934, located at Buitenzorg (Bogor), West Java. Our preliminary work based only on the macroscopic features in 2020 indicated the occurrence of Podostroma cf. cornu-damae in Central Kalimantan (Borneo). Hence, the current research aims to ensure the taxonomical identity of the macrofungi. Podostroma cornu-damae is confirmed based on morphological (macro-and microscopic) and molecular evidence. A brief description of the morphology of P. cornu-damae and the phylogenetic analyses inferred from the ITS sequence are provided. This study is the first comprehensive report of P. cornu-damae for Indonesia after nearly 100 years of hiatus. [ABSTRACT FROM AUTHOR]

Mushrooms have been considered an important part of human life due to their various benefits and potential. In Indonesia, many indigenous people get used to foraging and using wild mushrooms as part of their daily lives. To date, there was no update following prior local name checklist of wild mushroom and their uses in Indonesia. Thus, this review aims to provide the latest work on that information known so far in the country. A literature review was focusing on available publications containing the local names and the use of wild mushrooms in Indonesia. 107 mushrooms in total are known to have 170 local names with 36 of them having more than 1 indigenous name. Some of them: Coprinus spp., Polyporus spp., Schizophyllum commune, Scleroderma spp., Termitomyces spp., and Trametes spp. are known to have 5 local names for each region and ethnicity that uses them. 50 species of mushrooms in total are used as food and traditional medicine. The information was derived from 8 provinces and 8 tribes, of which West Kalimantan Province and Javanese ethnicity contributed to the highest number of it. The number of local names is expected to increase as more investigations are conducted in the near future. [ABSTRACT FROM AUTHOR]

Phallus is known as stinkhorn mushroom. Previous reports have shown that this genus is recognized by the size and color of the basidioma, which is a highly plastic morphological character that can potentially lead to misidentification. In Indonesia, no study has combined morphological and molecular analyses to identify Phallus. This study aimed to determine the identity of Phallus that has the orange color of an indusium found in Bekasi, West Java, Indonesia. The morphological characteristics were described by observing the macroscopic and microscopic features of fresh specimens. Molecular analyses were performed based on the internal transcribed spacer 4/5 region sequence. The results showed that specimen BO24430 was identified as Phallus multicolor with 100% similarity in the BLAST results and a 98% bootstrap value on the phylogenetic tree. This taxonomic placement was supported by morphological features, such as a campanulate pileus, yellowish to white pseudostipe, brown to whitish eggs, and ellipsoid spores. This study is the only record of P. multicolor in Indonesia with a herbarium voucher since Boedijn in 1932. In addition, the current study assessed the diversity of Phallus in Indonesia using morphological and molecular evidence. [ABSTRACT FROM AUTHOR]