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5. Fluorescent labelling of boar spermatozoa for quantitative studies on competitive sperm-oviduct binding

Author

Henning, Heiko H W, Batz-Schott, Julia, Grünther, Benita, Le Thi, Xuyen, Waberski, Dagmar, Henning, Heiko H W, Batz-Schott, Julia, Grünther, Benita, Le Thi, Xuyen, and Waberski, Dagmar

Abstract

In vitro sperm-oviduct binding assays enable assessment of the capacity of spermatozoa to form a 'reservoir' in the oviduct. Competitive approaches, such as experimental set-ups that test multiple males or semen samples simultaneously on the same tissue explants, are desirable because they reduce the likelihood of bias when using material from different females. Therefore, we established a fluorescent labelling technique that allows tagging and storage of spermatozoa before competitive studies of sperm-oviduct binding invitro. Fluorescent markers were tested for reliability and compatibility with parameters of boar spermatozoa viability. The addition of seminal plasma after density gradient centrifugation was essential to counteract centrifugation stress during the labelling procedure. It was demonstrated that sperm tagged with MitoTracker Green FM or MitoTracker Red FM can be successfully used in competitive sperm-oviduct binding studies. The assay was sensitive enough to indicate subtle effects of semen storage temperature on the ability of the spermatozoa to contribute to the female sperm reservoir.

Published
2019

7. Potential Health and Environmental Risks of Three-Dimensional Engineered Polymers

Author

de Almeida Monteiro Melo Ferraz, Marcia, Henning, Heiko H W, Ferreira da Costa, Pedro, Malda, Jos, Le Gac, Séverine, Bray, Fabrice, van Duursen, Majorie B M, Brouwers, Jos F, van de Lest, Chris H A, Bertijn, Ingeborg, Kraneburg, Lisa, Vos, Peter L A M, Stout, Tom A E, Gadella, Barend M, One Health Toxicologie, LS Klinische Reproductie, dES/dFAH FR, LS Voortplanting Inwendige Ziekten, Sub Reproductie mannelijk, LS Equine Muscoskeletal Biology, dES RMSC, LS Veterinaire biochemie, Sub MS-faciliteit, dB&C FR-RMSC RMSC, dB&C FR-RMSC FR, dFAH AVR, Sub Biologie van de mannelijke gameet, One Health Toxicologie, LS Klinische Reproductie, dES/dFAH FR, LS Voortplanting Inwendige Ziekten, Sub Reproductie mannelijk, LS Equine Muscoskeletal Biology, dES RMSC, LS Veterinaire biochemie, Sub MS-faciliteit, dB&C FR-RMSC RMSC, dB&C FR-RMSC FR, dFAH AVR, Sub Biologie van de mannelijke gameet, Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - USR 3290 (MSAP), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 (MSAP), and Université de Lille-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Letter, Health, Toxicology and Mutagenesis, UT-Hybrid-D, Estrogen receptor, Polyethylene glycol, 010501 environmental sciences, Diethyl phthalate, 01 natural sciences, Polymer engineering, 03 medical and health sciences, chemistry.chemical_compound, Transactivation, [CHIM]Chemical Sciences, Environmental Chemistry, Toxicology and Mutagenesis, Waste Management and Disposal, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, Water Science and Technology, chemistry.chemical_classification, Ecology, Chemistry, Polymer, Pollution, Cell biology, 030104 developmental biology, [CHIM.POLY]Chemical Sciences/Polymers, Health, Toxicity, Oviduct
Abstract

Polymer engineering, such as in three-dimensional (3D) printing, is rapidly gaining popularity, not only in the scientific and medical fields but also in the community in general. However, little is known about the toxicity of engineered materials. Therefore, we assessed the toxicity of 3D-printed and molded parts from five different polymers commonly used for prototyping, fabrication of organ-on-a-chip platforms, and medical devices. Toxic effects of PIC100, E-Shell200, E-Shell300, polydimethylsiloxane, and polystyrene (PS) on early bovine embryo development, on the transactivation of estrogen receptors were assessed, and possible polymer-leached components were identified by mass spectrometry. Embryo development beyond the two-cell stage was inhibited by PIC100, E-Shell200, and E-Shell300 and correlated to the released amount of diethyl phthalate and polyethylene glycol. Furthermore, all polymers (except PS) induced estrogen receptor transactivation. The released materials from PIC100 inhibited embryo cleavage across a confluent monolayer culture of oviduct epithelial cells and also inhibited oocyte maturation. These findings highlight the need for cautious use of engineered polymers for household 3D printing and bioengineering of culture and medical devices and the need for the safe disposal of used devices and associated waste.

Published
2018
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9. An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming

Author

Ferraz, Marcia A M M, Rho, Hoon Suk, Hemerich, Daiane, Henning, Heiko H W, van Tol, Helena T A, Hölker, Michael, Besenfelder, Urban, Mokry, Michal, Vos, Peter L A M, Stout, Tom A E, Le Gac, Séverine, Gadella, Bart M, Ferraz, Marcia A M M, Rho, Hoon Suk, Hemerich, Daiane, Henning, Heiko H W, van Tol, Helena T A, Hölker, Michael, Besenfelder, Urban, Mokry, Michal, Vos, Peter L A M, Stout, Tom A E, Le Gac, Séverine, and Gadella, Bart M

Abstract

Worldwide over 5 million children have been conceived using assisted reproductive technology, and research has concentrated on increasing the likelihood of ongoing pregnancy. However, studies using animal models have indicated undesirable effects of in vitro embryo culture on offspring development and health. In vivo, the oviduct hosts a period in which the early embryo undergoes complete reprogramming of its (epi)genome in preparation for the reacquisition of (epi)genetic marks. We designed an oviduct-on-a-chip platform to better investigate the mechanisms related to (epi)genetic reprogramming and the degree to which they differ between in vitro and in vivo embryos. The device supports more physiological (in vivo-like) zygote genetic reprogramming than conventional IVF. This approach will be instrumental in identifying and investigating factors critical to fertilization and pre-implantation development, which could improve the quality and (epi)genetic integrity of IVF zygotes with likely relevance for early embryonic and later fetal development.

Published
2018

10. Potential Health and Environmental Risks of Three-Dimensional Engineered Polymers

Author

One Health Toxicologie, LS Klinische Reproductie, dES/dFAH FR, LS Voortplanting Inwendige Ziekten, Sub Reproductie mannelijk, LS Equine Muscoskeletal Biology, dES RMSC, LS Veterinaire biochemie, Sub MS-faciliteit, dB&C FR-RMSC RMSC, dB&C FR-RMSC FR, dFAH AVR, Sub Biologie van de mannelijke gameet, de Almeida Monteiro Melo Ferraz, Marcia, Henning, Heiko H W, Ferreira da Costa, Pedro, Malda, Jos, Le Gac, Séverine, Bray, Fabrice, van Duursen, Majorie B M, Brouwers, Jos F, van de Lest, Chris H A, Bertijn, Ingeborg, Kraneburg, Lisa, Vos, Peter L A M, Stout, Tom A E, Gadella, Barend M, One Health Toxicologie, LS Klinische Reproductie, dES/dFAH FR, LS Voortplanting Inwendige Ziekten, Sub Reproductie mannelijk, LS Equine Muscoskeletal Biology, dES RMSC, LS Veterinaire biochemie, Sub MS-faciliteit, dB&C FR-RMSC RMSC, dB&C FR-RMSC FR, dFAH AVR, Sub Biologie van de mannelijke gameet, de Almeida Monteiro Melo Ferraz, Marcia, Henning, Heiko H W, Ferreira da Costa, Pedro, Malda, Jos, Le Gac, Séverine, Bray, Fabrice, van Duursen, Majorie B M, Brouwers, Jos F, van de Lest, Chris H A, Bertijn, Ingeborg, Kraneburg, Lisa, Vos, Peter L A M, Stout, Tom A E, and Gadella, Barend M

Published
2018

11. An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming

Author

LS Voortplanting Inwendige Ziekten, dES/dFAH FR, Sub Celbiologisch lab., dFAH AVR, LS Klinische Reproductie, Sub Biologie van de mannelijke gameet, Sub Reproductie mannelijk, dB&C FR-RMSC FR, Ferraz, Marcia A M M, Rho, Hoon Suk, Hemerich, Daiane, Henning, Heiko H W, van Tol, Helena T A, Hölker, Michael, Besenfelder, Urban, Mokry, Michal, Vos, Peter L A M, Stout, Tom A E, Le Gac, Séverine, Gadella, Bart M, LS Voortplanting Inwendige Ziekten, dES/dFAH FR, Sub Celbiologisch lab., dFAH AVR, LS Klinische Reproductie, Sub Biologie van de mannelijke gameet, Sub Reproductie mannelijk, dB&C FR-RMSC FR, Ferraz, Marcia A M M, Rho, Hoon Suk, Hemerich, Daiane, Henning, Heiko H W, van Tol, Helena T A, Hölker, Michael, Besenfelder, Urban, Mokry, Michal, Vos, Peter L A M, Stout, Tom A E, Le Gac, Séverine, and Gadella, Bart M

Published
2018

12. An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming

Author

Onderzoek Precision medicine, MDL onderzoek 1, Child Health, Onderzoek, Ferraz, Marcia A M M, Rho, Hoon Suk, Hemerich, Daiane, Henning, Heiko H W, van Tol, Helena T A, Hölker, Michael, Besenfelder, Urban, Mokry, Michal, Vos, Peter L A M, Stout, Tom A E, Le Gac, Séverine, Gadella, Bart M, Onderzoek Precision medicine, MDL onderzoek 1, Child Health, Onderzoek, Ferraz, Marcia A M M, Rho, Hoon Suk, Hemerich, Daiane, Henning, Heiko H W, van Tol, Helena T A, Hölker, Michael, Besenfelder, Urban, Mokry, Michal, Vos, Peter L A M, Stout, Tom A E, Le Gac, Séverine, and Gadella, Bart M

Published
2018

16. Potential Health and Environmental Risks of Three-Dimensional Engineered Polymers.

Author

de Almeida Monteiro Melo Ferraz M, Henning HHW, Ferreira da Costa P, Malda J, Le Gac S, Bray F, van Duursen MBM, Brouwers JF, van de Lest CHA, Bertijn I, Kraneburg L, Vos PLAM, Stout TAE, and Gadella BM

Abstract

Polymer engineering, such as in three-dimensional (3D) printing, is rapidly gaining popularity, not only in the scientific and medical fields but also in the community in general. However, little is known about the toxicity of engineered materials. Therefore, we assessed the toxicity of 3D-printed and molded parts from five different polymers commonly used for prototyping, fabrication of organ-on-a-chip platforms, and medical devices. Toxic effects of PIC100, E-Shell200, E-Shell300, polydimethylsiloxane, and polystyrene (PS) on early bovine embryo development, on the transactivation of estrogen receptors were assessed, and possible polymer-leached components were identified by mass spectrometry. Embryo development beyond the two-cell stage was inhibited by PIC100, E-Shell200, and E-Shell300 and correlated to the released amount of diethyl phthalate and polyethylene glycol. Furthermore, all polymers (except PS) induced estrogen receptor transactivation. The released materials from PIC100 inhibited embryo cleavage across a confluent monolayer culture of oviduct epithelial cells and also inhibited oocyte maturation. These findings highlight the need for cautious use of engineered polymers for household 3D printing and bioengineering of culture and medical devices and the need for the safe disposal of used devices and associated waste., Competing Interests: The authors declare no competing financial interest.

Published
2018
Full Text
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