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Your search keyword '"Hennager, Steven G"' showing total 16 results
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16 results on '"Hennager, Steven G"'

2. Equine piroplasmosis associated with Amblyomma cajennense ticks, Texas, USA

Author

Scoles, Glen A., Hutcheson, H. Joel, Schlater, Jack L., Hennager, Steven G., Pelzel, Angela M., and Knowles, Donald P.

Subjects
United States. Department of Agriculture, Disease transmission -- Health aspects, Babesiosis -- Health aspects, Horses -- Health aspects, Health
Abstract

Theileria equi (incertae sedis; Piroplasma equi Laveran, 1901) is one of the etiologic agents of equine piroplasmosis. This parasite infects equids worldwide, but a few countries (Australia, Great Britain, Japan, [...]

Published
2011
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3. Vaccination of Elk (Cervus canadensis) with Brucella abortus Strain RB51 Overexpressing Superoxide Dismutase and Glycosyltransferase Genes Does Not Induce Adequate Protection against Experimental Brucella abortus Challenge

Author

Nol, Pauline, Olsen, Steven C., Rhyan, Jack C., Sriranganathan, Nammalwar, McCollum, Matthew P., Hennager, Steven G., Pavuk, Alana A., Sprino, Phillip J., Boyle, Stephen M., Berrier, Randall J., and Salman, Mo D.

Subjects
wildlife vaccination, fluids and secretions, animal diseases, Brucella abortus, RB51, superoxide dismutase, glycosyltransferase, elk, O-side chain
Abstract

In recent years, elk (Cervus canadensis) have been implicated as the source of Brucella abortus infection for numerous cattle herds in the Greater Yellowstone Area. In the face of environmental and ecological changes on the landscape, the range of infected elk is expanding. Consequently, the development of effective disease management strategies for wild elk herds is of utmost importance, not only for the prevention of reintroduction of brucellosis to cattle, but also for the overall health of the Greater Yellowstone Area elk populations. In two studies, we evaluated the efficacy of B. abortus strain RB51 over-expressing superoxide dismutase and glycosyltransferase for protecting elk from infection and disease caused by B. abortus after experimental infection with a virulent B. abortus strain. Our data indicate that the recombinant vaccine does not protect elk against brucellosis. Further, work is needed for development of an effective brucellosis vaccine for use in elk. United States Department of AgricultureUnited States Department of Agriculture (USDA) This research was funded by the United States Department of Agriculture.

Published
2016

4. Vaccination of Elk (Cervus canadensis) with Brucella abortus Strain RB51 Overexpressing Superoxide Dismutase and Glycosyltransferase Genes Does Not Induce Adequate Protection against Experimental Brucella abortus Challenge

Author

Nol, Pauline, primary, Olsen, Steven C., additional, Rhyan, Jack C., additional, Sriranganathan, Nammalwar, additional, McCollum, Matthew P., additional, Hennager, Steven G., additional, Pavuk, Alana A., additional, Sprino, Phillip J., additional, Boyle, Stephen M., additional, Berrier, Randall J., additional, and Salman, Mo D., additional

Published
2016
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5. Comparison of Buffered, Acidified Plate Antigen to Standard Serologic Tests for the Detection of Serum Antibodies to Brucella abortus in Elk (Cervus canadensis)

Author

Clarke, P. Ryan, primary, Edwards, William H., additional, Hennager, Steven G., additional, Block, Jean F., additional, Yates, Angela M., additional, Ebel, Eric, additional, Knopp, Douglas J., additional, Fuentes-Sanchez, Antonio, additional, Jennings-Gaines, Jessica, additional, Kientz, Rebecca L., additional, and Simunich, Marilyn, additional

Published
2015
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7. Outbreak of equine piroplasmosis in Florida

Author

Short, Michael A., primary, Clark, Carol K., additional, Harvey, John W., additional, Wenzlow, Nanny, additional, Hawkins, Ian K., additional, Allred, David R., additional, Knowles, Donald P., additional, Corn, Joseph L., additional, Grause, Juanita F., additional, Hennager, Steven G., additional, Kitchen, Diane L., additional, and Traub-Dargatz, Josie L., additional

Published
2012
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15. Seroconversion and Abortion in Cattle Experimentally Infected with Brucellasp. Isolated from a Pacific Harbor Seal (Phoca Vitulina Richardsi)

Author

Rhyan, Jack C., Gidlewski, Thomas, Ewalt, Darla R., Hennager, Steven G., Lambourne, Dyanna M., and Olsen, Steven C.

Abstract

Previously unrecognized Brucellaspecies have been isolated from a number of marine mammals, including harbor seals (Phoca vitulina richardsi) in the Puget Sound area of the state of Washington. Because of the presence of dairy herds in proximity to the harbor seal populations, a study was conducted to determine the effects of the harbor seal Brucellaisolate in experimentally inoculated cattle. Six pregnant cattle were exposed by intravenous injection (n= 3) or intraconjunctival inoculation (n= 3). Two pregnant cows were intravenously injected with saline and served as controls. All of the cows receiving the Brucellaseroconverted on 1 or more tests commonly used for the detection of Brucella abortusinfection. Two of the cattle receiving the intravenous inoculation aborted, and brucellae were demonstrated in the fetuses and dams immediately following abortion. The remaining 4 Brucella-inoculated animals and their fetuses were culture negative for the organism at 14 weeks postinoculation. Results of this study indicate the marine mammal Brucellais capable of producing seroconversion and abortion in cattle but is less pathogenic in that species than B. abortus.

Published
2001
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16. Detection of Equine Antibodies to Babesia caballiby Recombinant B. caballiRhoptry-Associated Protein 1 in a Competitive-Inhibition Enzyme-Linked Immunosorbent Assay

Author

Kappmeyer, Lowell S., Perryman, Lance E., Hines, Stephen A., Baszler, Timothy V., Katz, Jonathan B., Hennager, Steven G., and Knowles, Donald P.

Abstract

ABSTRACTA competitive-inhibition enzyme-linked immunosorbent assay (cELISA) was developed for detection of equine antibodies specific forBabesia caballi. The assay used recombinant B. caballirhoptry-associated protein 1 (RAP-1) and monoclonal antibody (MAb) 79/17.18.5, which is reactive with a peptide epitope of a native 60-kDa B. caballiantigen. The gene encoding the recombinant antigen was sequenced, and database analysis revealed that the gene product is a rhoptry-associated protein. Cloning and expression of a truncated copy of the gene demonstrated that MAb 79/17.18.5 reacts with the C-terminal repeat region of the protein. The cELISA was used to evaluate 302 equine serum samples previously tested for antibodies to B. caballiby a standardized complement fixation test (CFT). The results of cELISA and CFT were 73% concordant. Seventy-two of the 77 serum samples with discordant results were CFT negative and cELISA positive. Further evaluation of the serum samples with discordant results by indirect immunofluorescence assay (IFA) demonstrated that at a serum dilution of 1:200, 48 of the CFT-negative and cELISA-positive serum samples contained antibodies reactive with B. caballiRAP-1. Four of five CFT-positive and cELISA-negative serum samples contained antibodies reactive withB. caballiwhen they were tested by IFA. These data indicate that following infection with B. caballi, horses consistently produce antibody to the RAP-1 epitope defined by MAb 79/17.18.5, and when used in the cELISA format, recombinant RAP-1 is a useful antigen for the serologic detection of anti-B. caballiantibodies.

Published
1999
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