1. Modulation of Tumor Cell Metabolism by Laser Photochemotherapy with Cisplatin or Zoledronic Acid In Vitro .
- Author
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Heymann PGB, Henkenius KSE, Ziebart T, Braun A, Hirthammer K, Halling F, Neff A, and Mandic R
- Subjects
- Blotting, Western, Bone Density Conservation Agents pharmacology, Cell Survival drug effects, Cell Survival radiation effects, Glycolysis drug effects, Glycolysis radiation effects, HSP70 Heat-Shock Proteins metabolism, HeLa Cells, Humans, Low-Level Light Therapy methods, Oxidative Phosphorylation drug effects, Oxidative Phosphorylation radiation effects, Photochemotherapy methods, Radiation-Sensitizing Agents pharmacology, Zoledronic Acid, Cisplatin pharmacology, Diphosphonates pharmacology, Imidazoles pharmacology, Lasers
- Abstract
Background/aim: Laser photochemotherapy is a new approach in cancer treatment using low-level laser therapy (LLLT) to enhance the effect of chemotherapy., Materials and Methods: In order to evaluate the effect of LLLT on tumor cells, HeLa cells were treated with cisplatin or zoledronic acid (ZA) followed by LLLT. Cell viability was evaluated with 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide assay. Oxidative phosphorylation and glycolysis were measured using extracellular flux analysis. Immunocytochemistry of heat-shock protein 70 (HSP70) and western blot analysis were performed., Results: LLLT alone increased viability and was associated with lower oxidative phosphorylation but higher glycolysis rates. Cisplatin and ZA alone lowered cell viability, glycolysis and oxidative phosphorylation. This effect was significantly enhanced in conjunction with LLLT and was accompanied by reduced oxidative phosphorylation and collapse of glycolysis., Conclusion: Our observations indicate that LLLT may raise the cytotoxicity of cisplatin and ZA by modulating cellular metabolism, pointing to a possible application in cancer treatment., (Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Published
- 2018
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