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8. Podocalyxin is a key negative regulator of human endometrial epithelial receptivity for embryo implantation

Author

Paule, Sarah G, primary, Heng, Sophea, additional, Samarajeewa, Nirukshi, additional, Li, Ying, additional, Mansilla, Mary, additional, Webb, Andrew I, additional, Nebl, Thomas, additional, Young, Steven L, additional, Lessey, Bruce A, additional, Hull, M Louise, additional, Scelwyn, Maxine, additional, Lim, Rebecca, additional, Vollenhoven, Beverley, additional, Rombauts, Luk J, additional, and Nie, Guiying, additional

Published
2021
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12. Embryo implantation is closely associated with dynamic expression of proprotein convertase 5/6 in the rabbit uterus

Author

Li Ying, Wang Jian, Heng Sophea, Sun Zhaogui, Nicholls Peter K, and Nie Guiying

Subjects
Gynecology and obstetrics, RG1-991, Reproduction, QH471-489
Abstract

Abstract Background Proprotein convertase 5/6 (PC5/6) is critical for embryo implantation in women, regulating both uterine epithelial receptivity and stromal cell decidualization. PC5/6 is likewise essential for implantation in mice, but involved only in decidualization. An alternative animal model is required to address the function of PC5/6 in the uterine epithelium. This study aimed to establish whether PC5/6 is associated with embryo implantation in rabbits. Methods Virgin New-Zealand white rabbits aged 3-4 moths were mated with males of the same strain, or pseudo-pregnancy induced. After mating, uterine tissues were collected over a 10 day (d) period (n = 3 per time point) for RNA, protein and histological analyses to determine the temporal and spatial uterine expression pattern of PC5/6 during the initial stages of pregnancy or induced pseudo-pregnancy. Results PC5/6 mRNA was up-regulated just prior to embryo attachment on d6, and the elevated expression was maintained throughout implantation on d6.5-10. Western analysis revealed a preferential up-regulation of PC5/6 in the implantation sites. Immunohistochemical analysis identified that both the amount and cellular localization of PC5/6 changed with increasing pregnancy stages. Before embryo attachment, PC5/6 was low and localised in the luminal and glandular epithelium. It increased on d6.5 in the basal glands and mucosal folds, and then strongly intensified on d7-10 in the multinucleated luminal symplasma and decidual cells at the site of embryo implantation. In contrast, the pseudo-pregnant uterus displayed relatively low and static PC6 mRNA expression throughout the 10 days, with no obvious changes in either PC5/6 level or cellular localization. Conclusions These findings demonstrate that embryo implantation in the rabbit is closely associated with dynamic expression of uterine PC5/6, and that the rabbit may be an appropriate model to investigate the function of PC5/6 in the uterine epithelium during embryo attachment.

Published
2011
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