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A method has been developed for the determination of [C.sub.70] fullerene in animal organs and tissues using reversed-phase high-performance liquid chromatography with ultraviolet detection combined with sample preparation consisting in the homogenization of samples, treatment of the homogenates with acetic acid, and extraction of fullerene with toluene. The recovery of [C.sub.70] fullerene from various organs was ~89-93%, the limit of detection is 75 ng/mL. Keywords: fullerenes, extraction, high-performance liquid chromatography DOI: 10.1134/S1061934815120138, [C.sub.70] fullerene together with other fullerenes is a representative of a new allotropic form of carbon. Along with [C.sub.60] fullerene, it forms a group of so-called light fullerenes, which are [...]

This review is devoted to the analytical application of carbohydrate-binding proteins called lectins. The nature of lectins and the regularities of their specificity with respect to simple sugars and complex carbohydrate-containing biomolecules are discussed. The main areas of the modern analytical application of lectins are described. Lectin-affinity chromatography, histo- and cytochemical approaches, lectin blotting, microarray, and biosensor technologies as well as microplate analysis are considered in detail. Data on the use of lectins for the detection of cells and microorganisms as well as the study of protein glycosylation are summarized. The large potential of lectins as components of analytical systems used for the identification of glycans and the characteristics of their structure are substantiated. [ABSTRACT FROM AUTHOR]

Background: Ciprofloxacin (CIP) and chloramphenicol (CAP) are relevant antibiotics of the fluoroquinolone (FQ) and amphenicol (AP) groups, respectively, widely used in veterinary practice and they contaminate agricultural products. In this study, a rapid and sensitive immunochromatographic assay (ICA) was developed for simultaneous detection of CIP and CAP in dairy products. The ICA was carried out in a direct competitive format using gold nanoparticles as a label., Results: The ICA developed here allowed for the detection of CIP and CAP in Triton X-100-containing buffered saline (PBST) within 15 min with instrumental detection limits of 20 pg mL -1 and 0.5 ng mL -1 , respectively, and with a visual detection limit of 5 ng mL -1 for both antibiotics. The ICA showed cross-reactivity (69-160%) to 19 antibiotics in the FQ group and no cross-reactivity (<0.1%) to 2 antibiotics of the AP group. The ICA allowed detection of CIP and CAP in a panel of dairy products by employing a simple procedure of preliminary sample preparation. The detection limits for the two antibiotics were the same as in PBST. The analytical recoveries of CIP and CAP in dairy products ranged from 83% to 120%., Conclusion: The analytical characteristics of the test system allow its use for the detection of antibiotics in milk and dairy products during all steps of production. © 2019 Society of Chemical Industry., (© 2019 Society of Chemical Industry.)