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Belimbing wuluh (Averrhoa bilimbi L.) is well known in Indonesia. This plant has wide medicinal properties, ranging from lowering cholesterol, uric acid, anti diabetic, antihypertensive, antioxidant, anti-inflammatory, cough medicine, thrush, and digestive disorders to treating acne. A number of studies have been conducted on the content of its fruit and leaves, but there is still a gap in the literature on the content of the flowers. This study was conducted to determine the effectiveness of the methanol extract of A. bilimbi flower as a natural antioxidant, to determine the chemical compounds contained in it by using GC-MS and to determine its potential utilization. Methanol extract of A. bilimbi crown and petals has the ability as an antioxidant with a very low category. The antioxidant activity of the crown flower is higher than that of the petals. The methanol extract of the crown and petals was dominated by Hexanedioic acid, γ-Sitosterol and Hexadecanoic acid compounds. Based on the compounds it contains, A. bilimbi extract can be used as an antimicrobial, anti diabetic, anticancer, lowering cholesterol levels and for maintaining skin health.

Pelestarian jenis-jenis tumbuhan yang memiliki khasiat obat atau sebagai sarana upacara agama sangat penting untuk dilakukan mengingat makin minimnya pengetahuan masyarakat tentang manfaat dan fungsi dari tumbuhan tersebut. Kebun Raya Gianyar adalah tempat konservasi tumbuhan dengan tujuan spesifik yang bertema “Taru Pramana dan Usada, Banten Bali Pulina (Tanaman upakara dan tanaman obat, yang digunakan dari zaman Bali kuno)”. Selain sebagai lahan konservasi ex-situ, Kebun Raya Gianyar diharapkan dapat menjadi perpustakaan hidup dan tempat belajar tentang pemanfaatan tumbuhan di Bali. Tujuan dari kegiatan ini adalah untuk mengkoleksi jenis-jenis tumbuhan yang memiliki manfaat obat maupun upacara, serta melihat peran serta masyarakat dalam konservasi tumbuhan khususnya tumbuhan yang bermanfaat sebagai bahan obat maupun upacara. Kegiatan eksplorasi berhasil mengkoleksi sebanyak 128 nomor koleksi yang terdiri dari 45 suku, 99 marga dan 119 jenis. Tanaman koleksi terbanyak diperoleh dari famili zingiberaceae. Dari kegiatan ini diketahui bahwa masyarakat di Kabupaten Gianyar berperan aktif dalam konservasi tumbuhan khususnya tumbuhan yang bermanfaat sebagai bahan obat maupun upakara.

Penelitian ini bertujuan untuk mengungkap aspek-aspek pengajaran keterampilan berbahasa dalam lomba drama Arab di Pondok Modern Darussalam Gontor. Penelitian ini merupakan penelitian kualitatif dengan metode analisis deskriptif. Metode pengumpulan data adalah observasi, wawancara, dan telaah dokumentasi. Metode analisis data yang digunakan adalah model Miles & Huberman. Hasil dari penelitian ini adalah: Kompetisi drama Arab di PMDG setidaknya memiliki tiga elemen utama yang mendukung pengajaran bahasa. Pertama, proses penyusunan naskah drama yang mendukung pengajaran keterampilan membaca dan menulis. Kedua, pelaksanaan latihan drama yang mendukung pengajaran keterampilan membaca dalam bentuk membaca dalam hati dan membaca nyaring, serta keterampilan berbicara dalam bentuk tanya jawab, menghafal dialog, dan bercerita. Ketiga, proses menonton drama yang mendukung pengajaran keterampilan menyimak berupa menyimak untuk mengetahui dan menyimak untuk menikmati dan menilai.

The use of fungicides plays an important role in controlling plant diseases caused by Fusarium. However, the development of environment friendly fungicides is considered more effective, which used the plant secondary metabolites. This study aims to find the latest alternative fungicides using Psidium Cattleianum Afzel plant extract, ex Sabine from the collection of the "Eka Karya" LIPI-Bali Botanical Garden, which can inhibit the growth of F. solani and to find the minimum dose of the plant extract in inhibiting growth of F. solani. The method used was early screening of plant and fungi, medium preparation for fusarium and minimum dose analyze. This study found that based on 19 plants tested, Psidium cattleianum proved effective in inhibiting the F. solani.  Further testing of psidium plants was carried out by looking at the minimum dose of inhibition against fusarium. The inhibition zone contained in the 1% treatment was greater than the concentrations of 0.75%, 0.50% and 0.25%. The research was continued at a concentration reduction of 0.20-0.005% with the results showing that a concentration of 0.20% to a concentration of 0.05% resulted in a moderate inhibition zone of 8 mm to 9.2 mm. Therefore, it can be concluded that the concentration of 1% is the concentration with the minimum dose of Psidium cattleianum in inhibiting the growth of the fungus Fusarium solani.

During 2022, outbreaks of foot-and-mouth disease (FMD) were reported across the islands of Indonesia, a country that had previously maintained an FMD-free (without vaccination) status since 1990. This report describes the near-complete genome sequence of a representative FMD virus collected from these cases belonging to the O/ME-SA/Ind-2001e lineage.

The outcome of SARS-CoV-2 infection is determined by multiple factors, including the viral, host genetics, age, and comorbidities. This study investigated the association between prognostic factors and disease outcomes of patients infected by SARS-CoV-2 with multiple S protein mutations. Fifty-one COVID-19 patients were recruited in this study. Whole-genome sequencing of 170 full-genomes of SARS-CoV-2 was conducted with the Illumina MiSeq sequencer. Most patients (47%) had mild symptoms of COVID-19 followed by moderate (19.6%), no symptoms (13.7%), severe (4%), and critical (2%). Mortality was found in 13.7% of the COVID-19 patients. There was a significant difference between the age of hospitalized patients (53.4 ± 18 years) and the age of non-hospitalized patients (34.6 ± 19) (p = 0.001). The patients’ hospitalization was strongly associated with hypertension, diabetes, and anticoagulant and were strongly significant with the OR of 17 (95% CI 2–144; p = 0.001), 4.47 (95% CI 1.07–18.58; p = 0.039), and 27.97 (95% CI 1.54–507.13; p = 0.02), respectively; while the patients’ mortality was significantly correlated with patients’ age, anticoagulant, steroid, and diabetes, with OR of 8.44 (95% CI 1.5–47.49; p = 0.016), 46.8 (95% CI 4.63–472.77; p = 0.001), 15.75 (95% CI 2–123.86; p = 0.009), and 8.5 (95% CI 1.43–50.66; p = 0.019), respectively. This study found the clade: L (2%), GH (84.3%), GR (11.7%), and O (2%). Besides the D614G mutation, we found L5F (18.8%), V213A (18.8%), and S689R (8.3%). No significant association between multiple S protein mutations and the patients’ hospitalization or mortality. Multivariate analysis revealed that hypertension and anticoagulant were the significant factors influencing the hospitalization and mortality of patients with COVID-19 with an OR of 17.06 (95% CI 2.02–144.36; p = 0.009) and 46.8 (95% CI 4.63–472.77; p = 0.001), respectively. Moreover, the multiple S protein mutations almost reached a strong association with patients’ hospitalization (p = 0.07). We concluded that hypertension and anticoagulant therapy have a significant impact on COVID-19 outcomes. This study also suggests that multiple S protein mutations may impact the COVID-19 outcomes. This further emphasized the significance of monitoring SARS-CoV-2 variants through genomic surveillance, particularly those that may impact the COVID-19 outcomes.

Radikal bebas adalah senyawa atau molekul yang menjadi salah satu penyebab utama berbagai macam penyakit degeneratif seperti hipertensi, jantung, diabetes, dan lainnya. Beberapa tahun terakhir, aktivitas antioksidan dari senyawa fenolik tumbuhan menjadi perhatian khusus bagi industri obat dan pangan sebagai pengganti antioksidan sintetik. Senyawa antosianin adalah salah satu senyawa tumbuhan yang dilaporkan memiliki aktivitas antioksidan yang tinggi. Antosianin adalah pigmen larut air yang secara alami terdapat pada berbagai jenis tumbuhan, pigmen ini memberikan warna pada bunga, buah, dan daun tumbuhan. Warna yang ditimbulkan oleh antosianin dapat bermacam-macam mulai dari merah, biru kemerahan, ungu, biru, hijau, hingga kuning tergantung dari tingkat keasaman (pH) lingkungan sekitar. Diduga tumbuhan yang memiliki warna merah memiliki kemampuan sebagai antioksidan. Kajian ini dilakukan untuk mengetahui aktivitas antioksidan dari beberapa tanaman yang memiliki warna merah seperti jahe merah, bayam hias merah dan azolla merah. Hasil penelitian menunjukkan bahwa dari ketiga ekstrak tumbuhan, ekstrak azolla merah memiliki kemampuan sebagai antioksidan paling tinggi, diikuti oleh ekstrak bayam hias merah dan azolla merah. Ketiga ekstrak memiliki kemampuan sebagai antioksidan dengan kategori sangat lemah.

Tebu ireng ( Saccharum officinarum L.) merupakan jenis tebu lokal yang memiliki ciri khusus yaitu warna batangnya yang hitam.Secara tradisional tebu ireng dimanfaatkan sebagai obat penyakit diabetes.Selain dapat dimanfaatkan secara tradisional sebagai obat diabetes, diyakini tebu ireng masih banyak menyimpan manfaat lain yang belum banyak diketahui. Studi ini dilakukan untuk mengetahui efektivitas ekstrak tebu ireng sebagai antioksidan dan antimikroba, ditinjau dari beberapa bagian tanamannya. Proses ekstraksi menggunakan metode maserasi dengan pelarut methanol, uji aktivitas antioksidan dilakukan dengan metode DPPH free radical scavenger menggunakan spektrofotometer UV-Vis. Pengujian aktivitas antimikroba dilakukan dengan menggunakan metode difusi agar (Kirby-Bauer disc diffusion method ). Hasil studi menunjukkan bahwa tebu ireng memiliki kemampuan sebagai antiokidan.Tingkat kepekatan warna dari tebu ireng berkorelasi dengan aktifitas antiokidannya.Ekstrak dari keseluruhan bagian tanaman tebu ireng efektif untuk menghambat pertumbuhan bakteri Staphylococcus aureus , Salmonella typhimurium , Pseudomonas aeruginosa dan Streptococcus mutans , namun tidak efektif menghambat pertumbuhan jamur Candida albicans .Tebu ireng mengandung pigmen Antosianin serta kaya akan serat pangan .

Dillenia serrata Thunb.is a member of the Dillenia clan which is endemic to Sulawesi. D. serrata fruit is a seasonal fruit and can be used as a cooking ingredient as a sour taste, raw material for several food products such as candied fruit and syrup, and has the potential to be processed into chips and jam. Traditionally, the leaves and bark of D. serrata are also used by the local community as a sprue medicine, fever, wound medicine, treating swelling or inflammation and treating vomiting of blood. It is believed that there are still many untapped benefits from this plant. This study was conducted to determine the effectiveness of D. serrata fruit extract as an antimicrobial that causes disease in humans, and to determine its effectiveness as an antioxidant. The antioxidant test was carried out using the DPPH method while the antimicrobial test was carried out by the agar diffusion method (Kirby-Bauer). The ripe Dillenia fruit was extracted using methanol, the extract was then diluted in various concentrations for the DPPH test. The results showed that the D. serrata extract had the ability as an antioxidant. The antioxidant activity of fresh fruit extracts was higher than that of dried fruit extracts. D. serrata extract is effective in inhibiting the growth of Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus mutans bacteria.

Background and Aim: Classical swine fever (CSF) is one of the primary diseases in animals in Indonesia, particularly areas that supply pig meat to the country, such as Karanganyar district, Central Java. The government has tried to prevent and control the disease by vaccination, but it has not yet given effective results. Therefore, another attempt to prevent the recurrence of CSF cases is to apply biosecurity in pig farms by looking for risk factors associated with on-farm and off-farm contact. This study aims to determine the contact rate and investigate the risk factors associated with on-farm and off-farm contact in commercial and smallholder pig farms in Karanganyar, Central Java, Indonesia, in the context of controlling CSF disease. Materials and Methods: This study used a cross-sectional study design in which the pig farm was designed as the observed epidemiological unit. The contact structure data were conducted by sampling using a two-stage random method. We selected Karanganyar district because it is the center of a pig farm in the Central Java Province and has many CSF cases in several years before. The study was conducted for more or less 1 month from August to September 2019. The contact data were collected from 37 smallholder farms and 27 commercial farms within interviews. Risk factors for contact with pigs were analyzed using logistic regression using the Statistix Program version 8.0.(www.statistix.com). Results: In comparison to smallholder farms, commercial farms had 2.38 and 3.32 times higher contact rate in outside farms and inside farms, respectively. Two factors increased the risk for on-farm contacts including commercials type farm (p=0.0012; odds ratio [OR]=8.32) with contact rate of 1.24 times/day and the time interval of CSF vaccination for 1-3 months (p=0.0013; OR=8.43) with contact rate of 0.98 times/day, and three factors increased the risk for off-farm contacts including the commercial farm type (p=0.012; OR=4.88) with 1.50 contact/day, the time interval of CSF vaccination for 1-3 months (p=0.036; OR=3.83) with 1.30 contact/day, and farmers with experience in pig husbandry

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a newly emerging virus well known as the major cause of the worldwide pandemic due to Coronavirus Disease 2019 (COVID-19). Major breakthroughs in the Next Generation Sequencing (NGS) field were elucidated following the first release of a full-length SARS-CoV-2 genome on the 10 January 2020, with the hope of turning the table against the worsening pandemic situation. Previous studies in respiratory virus characterization require mapping of raw sequences to the human genome in the downstream bioinformatics pipeline as part of metagenomic principles. Illumina, as the major player in the NGS arena, took action by releasing guidelines for improved enrichment kits called the Respiratory Virus Oligo Panel (RVOP) based on a hybridization capture method capable of capturing targeted respiratory viruses, including SARS-CoV-2; therefore, allowing a direct map of raw sequences data to SARS-CoV-2 genome in downstream bioinformatics pipeline. Consequently, two bioinformatics pipelines emerged with no previous studies benchmarking the pipelines. This study focuses on gaining insight and understanding of target enrichment workflow by Illumina through the utilization of different bioinformatics pipelines named as ‘Fast Pipeline’ and ‘Normal Pipeline’ to SARS-CoV-2 strains isolated from Yogyakarta and Central Java, Indonesia. Overall, both pipelines work well in the characterization of SARS-CoV-2 samples, including in the identification of major studied nucleotide substitutions and amino acid mutations. A higher number of reads mapped to the SARS-CoV-2 genome in Fast Pipeline and merely were discovered as a contributing factor in a higher number of coverage depth and identified variations (SNPs, insertion, and deletion). Fast Pipeline ultimately works well in a situation where time is a critical factor. On the other hand, Normal Pipeline would require a longer time as it mapped reads to the human genome. Certain limitations were identified in terms of pipeline algorithm, whereas it is highly recommended in future studies to design a pipeline in an integrated framework, for instance, by using NextFlow, a workflow framework to combine all scripts into one fully integrated pipeline.

BackgroundThe SARS-CoV-2 Omicron variant has replaced the previously dominant Delta variant because of high transmissibility. It is responsible for the current increase in the COVID-19 infectivity rate worldwide. However, studies on the impact of the Omicron variant on the severity of COVID-19 are still limited in developing countries. Here, we compared the outcomes of patients infected with SARS-CoV-2 Omicron and Delta variants and associated with prognostic factors, including age, sex, comorbidities, and smoking.MethodsWe involved 352 patients, 139 with the Omicron variant and 213 with the Delta variant. The whole-genome sequences of SARS-CoV-2 were conducted using the Illumina MiSeq next-generation sequencer.ResultsCt value and mean age of COVID-19 patients were not significantly different between both groups (Delta: 20.35 ± 4.07 vs. Omicron: 20.62 ± 3.75; p=0.540; and Delta: 36.52 ± 21.24 vs. Omicron: 39.10 ± 21.24; p=0.266, respectively). Patients infected with Omicron and Delta variants showed similar hospitalization (p=0.433) and mortality rates (p=0.565). Multivariate analysis showed that older age (≥65 years) had higher risk for hospitalization (OR=3.67 [95% CI=1.22-10.94]; p=0.019) and fatalities (OR=3.93 [95% CI=1.35-11.42]; p=0.012). In addition, patients with cardiovascular disease had higher risk for hospitalization (OR=5.27 [95% CI=1.07-25.97]; p=0.041), whereas patients with diabetes revealed higher risk for fatalities (OR=9.39 [95% CI=3.30-26.72]; p=ConclusionsOur study shows that patients infected with Omicron and Delta variants reveal similar clinical outcomes, including hospitalization and mortality. In addition, our findings further confirm that older age, cardiovascular disease, and diabetes are strong prognostic factors for the outcomes of COVID-19 patients.

Begonia adalah salah satu marga besar dalam kelompok Angiospermae yang memiliki kekhasan karakter daun asimetris. Keistimewaan Begonia terutama terletak pada daunnya yang indah, menjadikannya sangat cocok dijadikan tanaman hias. Perbanyakan Begonia dapat dilakukan dengan cara generatif dan vegetatif. Perbanyakan secara vegetatif adalah cara perbanyakan tanaman dengan menggunakan bagian dari tanaman seperti daun, batang, cabang dan akar. Keberhasilan kegiatan penyetekan pada tumbuhan dipengaruhi oleh berbagai faktor, yaitu jenis setek dan faktor lingkungan termasuk kelembaban temperatur dan cahaya serta nutrisi dari media tanam. Kualitas media tanam dapat ditingkatkan dengan penambahan pupuk. Penelitian ini bertujuan untuk mengetahui pengaruh beberapa jenis pupuk cair, serta cara perlakuannya terhadap pertumbuhan stek daun B. glabra. Hasil penelitian menunjukkan bahwa perlakuan POC/R berpengaruh nyata terhadap kecepatan tumbuhnya tunas dan tinggi tanaman. Perlakuan POC/R menunjukkan pertumbuhan yang lebih baik, yang berbeda nyata dengan perlakuan lainnya. Semua perlakuan pupuk berpengaruh terhadap kecepatan tumbuhnya tunas, namun tidak berpengaruh nyata terhadap parameter panjang akar. Perbedaan jenis pupuk cair dan cara perlakuannya memberikan hasil yang berbeda pula.

Leucorrhoea is one of common disease in Indonesia that caused by infections of fungus Candida albicans. In dealing with this fungi, the synthetic and even an natural compound had used. Based on pre-screening data of the plants from botanical garden of Eka Karya Lipi Bali, there found apotential plant. This study aims to find the latest alternative treatment using the extract of Montanoa hiscifolia Benth plant from the collection of the "Eka Karya" LIPI Bali Botanical Garden which can inhibit fungal growth C. albicans. This research method begins with the literature skrining process using T.K Liem books and direct plant selection. After that proceed with the extraction process of 10 plant samples dose using the evaporator. The plant extracts were then tested directly on 4 types of fungi, from Eka Karya Lipi Bali’s collected.The dose used in ths study were 3%, 2%, 1% and 0.5% respectively. The final minimum dosage test was carried out using various concentrations of M. hiscifolia Benth plant extract which is the only type of best plant extract that can inhibit growth of C. albicans. Therefore, the minimum dosage test was carried out by reducing the concentration from 3 % to 0,0001 % to determine the minimum dose of extract M. hiscifolia Benth inhibiting the fungus C. albicans. This study found that at a concentration of 3 % was showed the best results for inhibition zone. On the contrary, at the concentration of 0,0001%, whereas the area of the inhibition zone was 7,2 mm and categorized as a medium inhibitor. At the last, we were compared the dose of commercial product that oftenly used as Leucorrhoea treatment with M. hibiscifolia benth’s bioactive and it proven the M. hibiscifolia benth having a hight potential in order to cope the C. albicans. Further research might as well to check it bioactive compound that specifically play important role against the C. Albican. Beside, eventually develop this plant extract into a real product in order to be used as easy as for the people who need it.

Background and Aim: In Indonesia, highly pathogenic avian influenza (HPAI) H5N1 outbreaks in poultry are still reported. The disease causes a decrease in egg production and an increase in mortality; this has an impact on the economic losses of farmers. Several studies have considered that ducks play a role in the HPAI endemicity in the country; however, little is known about whether or not the type of duck farming is associated with HPAI H5 virus infection, particularly within clade 2.3.2.1c, which has been predominantly found in poultry since 2014. A cross-sectional study was conducted to determine the HPAI seroprevalence for H5 subtype clade 2.3.2.1c in laying ducks that are kept intensively and nomadically and to determine the associated risk factors. Materials and Methods: Forty-nine duck farmers were randomly selected from ten sub-districts in Purbalingga District, Central Java, Indonesia; a cross-sectional study was implemented to collect field data. Based on an expected HPAI prevalence level of 10%, estimated accuracy of ± 5%, and 95% confidence interval (CI), the total sample size was calculated at 36 individuals. Samples must be multiplied by 7 to reduce bias; thus, 252 ducks were taken as samples in this study. Considering that the maintenance and duck handling were uniform and farmers complained that the effect of activity to take duck samples would reduce egg production, this study only took samples from 245 ducks (oropharyngeal swabs and serum). Those samples were taken from five birds on each farm. Hemagglutination inhibition tests examined the serum samples for HPAI H5 Clade 2.3.2.1c, and pool swab samples (five swabs in one viral media transport) were examined by real-time reverse transcription-polymerase chain reaction (qRT-PCR) test for influenza Type A and H5 subtype virus. Information regarding farm management was obtained using a questionnaire; face-to-face interviews were conducted with the duck farmers using native Javanese language. Results: Serum and swabs from 245 ducks were collected in total. For individual birds, 54.69% (134/245) of serum samples were H5 seropositive. Seroprevalence among nomadic ducks was 59.28% (95% CI: 0.48-0.61), which was higher than among intensively farmed ducks (48.57%, 95% CI: 0.38-0.58). Farm-level seroprevalence was 50% (95% CI: 0.30-0.69) for nomadic ducks but only 28.57% (95% CI: 0.11-0.51) for intensively farmed ducks. The farm-level virus prevalence (proportion of flocks with at least one bird positive for influenza Type A) was 17.85% (95% CI: 0.07-0.35) for nomadic ducks and 4.76% (1/21) for intensively farmed ducks (95% CI: 0.008-0.23). All influenza Type A positive samples were negative for the H5 subtype, indicating that another HA subtype AI viruses might have been circulating in ducks in the study area. A relationship between duck farms that were H5 seropositive and their maintenance system was present; however, this relationship was not significant, the nomadic duck system detected 2 times higher H5-seropositive ducks than the intensive farming system (OR: 2.16, 95% CI: 0.33-14.31). Conclusion: This study found that the seroprevalence of HPAI in the duck population level in Purbalingga was 54.69% and demonstrated that the nomadic duck farming system was more likely to acquire HPAI H5 infection than the intensive farming duck system. Other risk factors should be further investigated as the diversity of the farming system is partially related to HPAI H5 infection.

Dioscorea hispida atau yang lebih dikenal dengan nama Gadung adalah salah satu jenis tumbuhan dari suku Dioscoreaceae. Umbi Dioscorea memiliki peran yang unik dalam masyarakat baik sebagai bahan pangan, maupun obat tradisional. Kandungan karbohidrat dan protein yang tinggi dari Dioscorea menjadikannya salah satu bahan pangan alternatif. Umbi D. hispida dapat dikonsumsi, dijadikan keripik atau makanan olahan pengganti nasi setelah diolah terlebih dahulu. Umbi dari D. hispida diketahui beracun karena mengandung alkaloid, karena itu sering dimanfaatkan oleh masyarakat sebagai bahan insektisida dan rodentisida alami. Selain itu umbi Dioscorea dapat pula dimanfaatkan sebagai obat salah satunya karena memiliki kandungan steroidal sapogenin. Tujuan dari penelitian ini adalah untuk mengetahui variasi kandungan gizi D. hispida yang berasal dari beberapa wilayah di Pulau Bali dan Lombok, serta untuk mengetahui apakah terdapat variasi genetika pada D. hispida yang mungkin dipengaruhi oleh adanya perbedaan tempat tumbuh pada kedua pulau tersebut. Dari hasil penelitian yang dilakukan dapat disimpulkan bahwa perbedaan tempat tumbuh tidak berpengaruh nyata terhadap kualitas kandungan gizi D. hispida. Perbedaan tempat tumbuh hanya berpengaruh terhadap kandungan kalsium oksalat, dimana kandungan kalsium oksalat D. hispida yang berasal dari Bali nyata lebih rendah dibandingkan dengan yang berasal dari Lombok. Hasil uji lanjutan pada level Provinsi menunjukkan bahwa kadar kalsium oksalat dari Bali Timur lebih rendah dari Bali Barat dan Utara. DNA kloroplast trnL-trnF dan DNA inti pgiC yang diuji tidak menunjukkan adanya variasi sekuensnya.

Boenninghausenia albiflora is a well-known plant for being used in various traditional treatments. The plant is also reported to contain an essential oil which has a distinctive and pungent aroma. The present study aimed at investigating extraction yields, phytochemical compounds and antioxidants, and potential uses of B. albiflora leaves extracted with acetone solvent. Bioactive compounds of the leaf extract of B. albiflora were identified by GC-MS analysis while the antioxidant assay was measured by DPPH method. The extract obtained from acetone contained 30 compounds including coumarin group, 2-Isoprenyl-2,3-dihydro-furo [3,2-G] chromen-7-one (22.89% w/v) as the highest percentage compound. Other coumarins such as suberosin and dehydrogeijerin were also identified in this study. The IC50 values of the leaves extract of B. albiflora was 194.3 ppm which was higher than propyl gallate. Thus indicating B. albiflora as potential sources of anti-cancer, anti-inflammatory and antioxidant.

Background: Severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) Delta variant (B.1.617.2) has been responsible for the current increase in Coronavirus disease 2019 (COVID-19) infectivity rate worldwide. We compared the impact of the Delta variant and non-Delta variant on the COVID-19 outcomes in patients from Yogyakarta and Central Java provinces, Indonesia.Methods: In this cross-sectional study, we ascertained 161 patients, 69 with the Delta variant and 92 with the non-Delta variant. The Illumina MiSeq next-generation sequencer was used to perform the whole-genome sequences of SARS-CoV-2.Results: The mean age of patients with the Delta variant and the non-Delta variant was 27.3 ± 20.0 and 43.0 ± 20.9 (p = 3 × 10−6). The patients with Delta variant consisted of 23 males and 46 females, while the patients with the non-Delta variant involved 56 males and 36 females (p = 0.001). The Ct value of the Delta variant (18.4 ± 2.9) was significantly lower than that of the non-Delta variant (19.5 ± 3.8) (p = 0.043). There was no significant difference in the hospitalization and mortality of patients with Delta and non-Delta variants (p = 0.80 and 0.29, respectively). None of the prognostic factors were associated with the hospitalization, except diabetes with an OR of 3.6 (95% CI = 1.02–12.5; p = 0.036). Moreover, the patients with the following factors have been associated with higher mortality rate than the patients without the factors: age ≥65 years, obesity, diabetes, hypertension, and cardiovascular disease with the OR of 11 (95% CI = 3.4–36; p = 8 × 10−5), 27 (95% CI = 6.1–118; p = 1 × 10−5), 15.6 (95% CI = 5.3–46; p = 6 × 10−7), 12 (95% CI = 4–35.3; p = 1.2 × 10−5), and 6.8 (95% CI = 2.1–22.1; p = 0.003), respectively. Multivariate analysis showed that age ≥65 years, obesity, diabetes, and hypertension were the strong prognostic factors for the mortality of COVID-19 patients with the OR of 3.6 (95% CI = 0.58–21.9; p = 0.028), 16.6 (95% CI = 2.5–107.1; p = 0.003), 5.5 (95% CI = 1.3–23.7; p = 0.021), and 5.8 (95% CI = 1.02–32.8; p = 0.047), respectively.Conclusions: We show that the patients infected by the SARS-CoV-2 Delta variant have a lower Ct value than the patients infected by the non-Delta variant, implying that the Delta variant has a higher viral load, which might cause a more transmissible virus among humans. However, the Delta variant does not affect the COVID-19 outcomes in our patients. Our study also confirms that older age and comorbidity increase the mortality rate of patients with COVID-19.

Melaleuca trichostachya Lindl. merupakan tumbuhan yang termasuk famili Myrtaceae yang berasal dari Benua Australia. Daun dari tumbuhan ini mengandung minyak atsiri. Salah satu manfaat dari minyak atsiri yaitu sebagai anti jamur. Penelitian bertujuan mengetahui rendemen minyak atsiri daun M. trichostachya, kualitas, kandungan senyawa, serta daya hambatnya terhadap jamur Candida albicans. Daun M trichostachya segar dan kering sebanyak 200 g diekstraksi dengan metode destilasi uap dengan 3 kali ulangan. Rendemen minyak atsiri dihitung, kualitas diuji, senyawa penyusun dianalisis dengan Gas Chromatography Mass Spectrometry (GC-MS). Daya hambat minyak atsiri terhadap C. albicans diuji dengan kertas cakram pada media PDA dengan konsentrasi 25% b/b. Hasil penelitian menunjukkan bahwa rendemen minyak atsiri M. trichostachya daun segar adalah 1,00±0,058% sedangkan rendemen daun kering lebih rendah, yaitu 0,77±0,038%. Kualitas minyak atsiri yang dihasilkan termasuk kategori baik dari segi organoleptik, kemurnian, dan bilangan asam. Senyawa utama terdiri dari eucalyptol dan alpha terpinolene serta memiliki daya hambat terhadap jamur C. albicans.

Meliaceae are popularly used to produce terpenoid and limonoid compounds. These compounds have the potential as antioxidants, antimicrobials, insecticides, antipyretics, and anthelmintics. This research aims to determine the antioxidant and antimicrobial activity of Meliaceae leaves extract of Eka Karya Bali Botanic Garden plants collection. The dried leaves of 15 species of Meliaceae were extracted by methanol. In vitro antimicrobial tests were carried out on agar media inoculated by selected microbe. On the other hand, the antioxidant activity was assayed by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging. As a result, Aphanamixis polystachya against Aspergillus niger (35.21 mm) and Cladosporium sp. (46.21), Toona hexandra against Fusarium solani (37.31 mm), Dysoxylum cauliflorum against Candida albicans (38.19 mm), Chisocheton pentandrus against Salmonella typhimurium (39.53 mm), Sandoricum koetjape against Staphylococcus aureus (50.07 mm), and Toona hexandra against Streptococcus nutans (42.25 mm) exhibited the strongest inhibitory effect. Furthermore, the antioxidant screening showed that Cipadessa baccifera, C. pentandrus, and Sandoricum koetjape exhibited strong antioxidant activity, while T. ciliata showed a very strong antioxidant activity with IC50 33.64 μg/mL. This study reveals an initial screening of the potential of various types of Meliaceae as a source of antioxidants and antibacterials. Furthermore, this information can be used as a new alternative for pharmaceutical companies and the industrial sector in the development of new products.

Background: Recent studies focusing on the association of SARS-CoV-2 variants of concern (VOC) on COVID-19 outcomes have been reported. However, studies of the impact of multiple mutations within the spike (S) protein of SARS-CoV-2 on COVID-19 illness are limited. This study determined the association between multiple mutations within the S protein, prognosis factors, and the disease outcomes of SARS-CoV-2 infection. Methods: We included 51 COVID-19 patients from Yogyakarta and Central Java, Indonesia. Whole genome sequences of SARS-CoV-2 were determined by the Illumina MiSeq next-generation sequencer, followed by the phylogenetic analysis of 170 full-genomes of SARS-CoV-2 from different regions. We analyzed characteristics of COVID-19 patients and multiple mutations in association with different outcomes.Results: Among 51 patients, the clinical manifestations of COVID-19 were as follows: without any symptoms (13.7%), mild (47%), moderate (19.6%), severe (4%), critical (2%), and died (13.7%). The age of hospitalized patients (53.4 ± 18 years) was higher than non-hospitalized patients (34.6 ± 19) (p=0.001). A significant association between diabetes, hypertension, and anticoagulant and the hospitalization of patients was noted with p-value of 0.039 (OR=4.47 [95% CI=1.07-18.58]), 0.001 (OR=17 [95% CI=2-144]), and 0.02 (OR=27.97 [95% CI=1.54-507.13]), respectively; whereas a strong association between patients’ age, diabetes, anticoagulant, and steroid with the mortality of patients was revealed with p-value of 0.016 (OR=8.44 [95% CI=1.5-47.49]), 0.019 (OR=8.5 [95% CI=1.43-50.66]), 0.001 (46.8 [95% CI=4.63-472.77]), and 0.009 (OR=15.75 [95% CI=2-123.86]), respectively. All viruses contained the D614G variant, except one case. Accordingly, the samples were classified as the following clade: L (2%), GH (84.3%), GR (11.7%), and O (2%). Besides the D614G, the most common variants in the S protein were L5F (18.8%), V213A (18.8%), and S689R (8.3%). There was no significant association between multiple S protein variants with either hospitalization or mortality of COVID-19 (p=0.11 and 0.69, respectively). Multivariate analysis showed that hypertension and anticoagulant were the strong factors affecting the hospitalization and mortality of patients with COVID-19 with a p-value of 0.009 (OR=17.06 [95% CI=2.02-144.36]) and 0.001 (OR=46.8 (95% CI=4.63-472.77), respectively. Interestingly, the multiple S protein variants almost reached a significant level affecting the hospitalization of patients (p=0.07). Phylogenetic analysis showed that although most of the viruses from this study belonged to clade GH, none were detected as the variant of concern (VOC) and the variant of interest (VOI) of SARS-CoV-2.Conclusions: Here, we show for the first time the association between SARS-CoV-2 mutations within the S protein besides the VOC with the COVID-19 outcomes. Our findings suggest that multiple mutations in the S protein might affect the severity of COVID-19. Our study further suggests the importance of genomic surveillance to monitor SARS-CoV-2 variants, particularly those that might influence the outcomes of COVID-19 patients.

In July 2016, an avian influenza outbreak in duck farms in Yogyakarta province was reported to Disease Investigation Center (DIC), Wates, Indonesia, with approximately 1,000 ducks died or culled. In this study, two avian influenza (AI) virus subtypes, A/duck/Bantul/04161291-OR/2016 (H5N1) and A/duck/Bantul/04161291-OP/2016 (H9N2) isolated from ducks in the same farm during an AI outbreak in Bantul district, Yogyakarta province, were sequenced and characterized. Our results showed that H5N1 virus was closely related to the highly pathogenic AI (HPAI) H5N1 of clade 2.3.2.1c, while the H9N2 virus was clustered with LPAI viruses from China, Vietnam and Indonesia H9N2 (CVI lineage). Genetic analysis revealed virulence characteristics for both in avian and in mammalian species. In summary, co-circulation of HPAI-H5N1 of clade 2.3.2.1c and LPAI-H9N2 was identified in a duck farm during an AI outbreak in Yogyakarta province, Indonesia. Our findings raise a concern of the potential risk of the viruses, which could increase viral transmission and/or threat to human health. Routine surveillance of avian influenza viruses should be continuously conducted to understand the dynamic and diversity of the viruses for influenza prevention and control in Indonesia and SEA region.

Background and Aim: A previous study divided Indonesian bovine viral diarrhea virus (BVDV)-1 into subgenotypes BVDV-1a to BVDV-1d based on the partial NS5B gene using strain Bega as reference for BVDV-1a. In fact, it is clustered into BVDV-1c with strain Bega-like Australia. BVDV genotyping has been done on isolates from Jakarta, West and Central Java, but East Java isolates have not been genotyped. This study aimed to analyze genetic variability and amino acid residues in the nucleotide-binding pocket of the NS5B gene from infected cattle. Materials and Methods: Samples were obtained from the Sera Bank originating from active and passive surveillance of cattle that had been tested for BVDV antigen from 2013 to 2017. Detection of the p80 antibody and BVDV genotyping was carried out using ELISA and nested-multiplex-polymerase chain reaction (PCR), respectively. We defined 15 nested PCR products for partial sequencing of NS5B. Those field samples were selected from each location and year using proportional calculation as a representative sample. Homological and phylogenetic analyses of the partial NS5B gene were performed using BLAST and MEGA version 6. Results: Based on the phylogenetic tree analysis using 360 nucleotides as the partial NS5B gene, Indonesian BVDV-1 isolates from Central and East Java were subdivided to BVDV-1a (n=9), BVDV-1b (n=1), and BVDV-1c (n=5). In the present study, the homology of BVDV subgenotype -1a, -1b, and -1c was compared to the BVDV GenBank data and found 90-93%, 93%, and 92-95% respectively with the average pairwise distance of 0.207. A point mutation was shown at R283K of all BVDV isolates based on the sequence of three amino acid residues R283, R285, and I287 in the nucleotide-binding pocket as a part of the encoded RNA-dependent RNA polymerase. Conclusion: This study revealed the genetic variability of BVDV infecting cattle in Central Java and East Java, Indonesia, the subtypes BVDV-1a, BVDV-1b, BVDV-1c, and a point mutation at the R283K residue.

Alstonia macrophylla Wall. ex G.Don is a native plant to South East Asia, belonging to Alstonia genus. This species has been reported to have numerous natural chemical compound which perfom multiple pharmacological and biological activities. The aim of this study was to investigated the phytochemical properties of the acetone extract of the flower of lstonia macrophylla Wall. ex G.Don. This is very interesting because phytochemical properties of its flower had been never reported yet. Alstonia macrophylla was harvested from the Natural Forest of Punik, Batu Dulang Village, Batulanteh Subdistrict, Sumbawa Regency, West Nusa Tenggara, Indonesia on Mei 2015. Acetone flower extract of A. macrophylla was analyzed with a GC-MS method to determine the chemical components. Result of GC–MS chromatogram revealed that there were 30 identified components in this extract. The major compounds were Cycloartenol acetate (17.11 %); 5H-1-Pyrindine (12.44 %); Lupeyl acetate (10.12%); Oleic acid (6.08 %); Benzenesulfonic acid, 4-hydroxy- (4.25 %); p-n-Amylphenol (4.23 %); and 4-Methylindole (4.22 %). Here, We reported the first study of phytochemical properties of A. macrophylla. This study help to understand further detail the potential of bioactive compound of A. macrophylla.

The study is aimed to provide a comprehensive account on authentication of herbal drug named as Tukhm-e-balango (Lallemantia royleana Benth.) from the seeds of Ocimum basilicum by using microscopic, pharmacognostic, and phytochemical characterization. The crude medicinal plants and their parts are often adulterated or substituted in market due to improper identification by the consumers while among herbal plant sellers, taxonomic confusion is caused due to morphological similarities of the plant parts and lack of a standard identification system.In microscopy, both herbarium and fresh specimens were studied using qualitative and quantitative morphological characteristics of leaves, seeds, and pollen. For pharmacognosy, solubility, fluorescence, and physicochemical characterizers were analyzed whereas a total phenolic and flavonoids contents was determined in addition to DPPH radical scavenging activity. In current study, microscopic, pharmacognostic, and phytochemical characterization clearly differentiated L. royleana from O. basilicum. The major problem in herbal drug industry is caused due to confusion and controversy of certain synonyms used for more than one or two drugs. Sometimes, under the same common or local name, entirely different taxa are being sold in herbal markets. It is concluded that correct and proper identification of medicinal plants is very crucial to ensure the safety and efficacy of herbal medicines, as many medicinal plants are intentionally or unintentionally adulterated with similar species or varieties. In herbal market, the seeds of L. royleana are adulterated with seeds of O. basilicum due to their similar morphology.

Pterospermum javanicum Jungh. (Bayur) is a species belongs to the Pterospermum genera (Malvaceae). Several species of Pterospermum had been reported for their ethnobotanical usage, but the studies about ethnobotany information of Bayur and its secondary metabolite compounds were still limited which have been published. This study aimed to observe the ethnobotanical usage of Bayur and to examine the phytochemical contents of the acetone extract of Bayur flower from West Lombok. The ethnobotany information of Bayur was obtained through interviews with local people and more information on the ethnobotanical records of P. javanicum (Bayur) was conducted by reviewing the scientific literature. The chemical compounds of Bayur flower were analysed by the GC-MS method. The results revealed that Bayur was used by the Sasak community around Gunung Rinjani National Park West Lombok for various purposes, such as traditional medicines, beverages, rigging, and building material. The phytochemical analysis showed that the acetone extract of Bayur flower from West Lombok contained 38 identified chemical components, representing 93.78% of the total compounds. The major contents of them were Lupeyl acetate (10.68%), p-n-Amylphenol (8.16%), Lauric acid (7.31%), N-(Methyl-d2)- Aniline (5,82%), and Pentanal (5.07%). This report was the first publication about the phytochemical contents of Bayur flower. It is expected that this study gives further information on the potentials of Bayur, especially about its secondary metabolite to support and prove the truth of the cultural concept of society in utilizing Bayur as a medicinal plant.

Sutomo, Iryadi R, Darma ID, Wibawa IPAH, Rahayu A, Hanum SF, Rizal S, Novamizanti L, Raharjo J. 2021. Short Communication: Plant diversity utilization, and land cover composition in the Subak Jatiluwih, Bali, Indonesia. Biodiversitas 22: 1424-1432. Subak is water management or irrigation system for paddy fields in Bali Island and it has been assigned as a UNESCO’s World Heritage Site. At a landscape level, it comprises several components which are forests, terraced paddy landscape, rice fields, villages and temples. Subak in Jatiluwih Village, Tabanan Regency depicts an area characterized by its natural appearance in the form of a vast rice valley with a dike in stratum following its natural contours (frequent terraces). This paper aimed to explore plant diversity in various vegetations around Subak Jatiluwih as well as their usage in the daily living of the local community. We also explore the potential application of drone for classifying the landscape patterns of the Subak.Vegetation sampling to record plant diversity was done using purposive sampling, and drone or Unmanned Aerial Vehicle (UAV) was used to map the Subak Jatiluwih landscape. The potential usage of each species was obtained through interview with key respondents, and the level of usage of each species was analyzed using the BIV (Benefit Index Value). Tegalan area shows the highest number plant diversity in Subak Jatiluwih area. Furthermore, there are four species of plants that have the highest BIV namely: Cocos nucifera L., Psidium guajava L., Areca catechu L. and Musa × paradisiaca L.. Various plant uses by the locals include for animal feed, building, ceremony, craft, and food and medicinal purposes. The landscape in Subak Jatiluwih is dominated the vast valley of rice fields that has strata following its natural contours. These conditions provide opportunities to applied the conservation strategy based on cultural and custom values.

Minyak atsiri, atau yang juga popular dengan nama essential oil, sangat terkenal dengan ciri khas aromatik yang kuat. Manfaatnya pun juga sangat banyak, mulai dari antiseptik, analgesik, antidepresi, hingga antijamur. Oleh karena itu, tidak mengherankan jika minyak yang bersifat volatil ini umum digunakan di berbagai bidang industri dan telah menjadi komoditas yang bernilai ekonomi tinggi. Pada tahun 2014–2015, tim khusus dari Kebun Raya “Eka Karya” Bali telah melakukan eksplorasi ke Gunung Batulanteh di Kabupaten Sumbawa dan Desa Sembalun di Kabupaten Lombok Timur. Melalui eksplorasi tersebut, ratusan aksesi tumbuhan berpotensi penghasil minyak atsiri berhasil dikoleksi. Buku ini merangkum sekaligus mendokumentasikan data-data ilmiah terkait tumbuhan tersebut. Berbeda dengan terbitan lain yang sejenis, buku ini memiliki keunggulan dalam menyajikan informasi ilmiah yang komprehensif, mulai dari deskripsi botani, kandungan senyawa kimia tumbuhan, proses penyulingan, hingga pengembangannya. Buku ini sangat cocok untuk dibaca berbagai kalangan, seperti peneliti, dosen, mahasiswa, serta praktisi dan penghobi minyak atsiri.