1,741 results on '"Helms J"'
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2. Climate, geography, and the mating phenology of ants
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Helms, J. A.
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- 2023
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3. Retinyl Ester Analysis by Orbitrap Mass Spectrometry
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Jansen, Jeroen W. A., primary, Haaker, Maya W., additional, Zaal, Esther A., additional, and Helms, J. Bernd, additional
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- 2023
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4. A critical analysis on quality‐of‐life in women with visible facial disfigurements.
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Okine, E., Helms, J. A., and Luhrmann, T.
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Aims: This study aimed to examine whether surgical treatment for a facial disfigurement influenced an individual's quality of life. Methods and Results: One‐on‐one interviews were conducted with the aim of synthesizing participant's medical experiences into common themes. Additionally, participants completed the World Health Organization's Quality of Life Brief Version (WHOQOL‐BREF) questionnaire. The WHOQL‐BREF is a standardized testing instrument with four domains of 26 questions, meant to analyze participants' overall quality of health, physical health, psychological status, social relationships, and environmental health. Our study revealed that women with visible facial differences experienced a quality of life below the average of the general population. However, in those who reported above‐average quality of life, a key theme emerged: active participation in the choice to undergo surgical treatment. Participants who felt this sense of agency in the decision‐making process also reported a more positive healthcare experience. They felt more respected by others, indicating a strong connection between personal agency, surgical choices, and overall well‐being. Conclusions: These findings reveal that personal agency plays an important role in the decision‐making process for patients undergoing surgical treatment for facial differences, as it improves quality of life and has a positive impact on overall healthcare experience and well‐being. [ABSTRACT FROM AUTHOR]
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- 2024
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5. FOREST RESTORATION PROGRESS AND LESSONS LEARNED IN GUNUNG PALUNG NATIONAL PARK, INDONESIA
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Fawzi, NI, Helms, J, Emerson, A, and Wibawanto, MA
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- 2020
6. Histopathological features in fatal COVID-19 acute respiratory distress syndrome
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Merdji, H., Mayeur, S., Schenck, M., Oulehri, W., Clere-Jehl, R., Cunat, S., Herbrecht, J.-E., Janssen-Langenstein, R., Nicolae, A., Helms, J., Meziani, F., and Chenard, M.-P.
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- 2021
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7. Early activation of hepatic stellate cells induces rapid initiation of retinyl ester breakdown while maintaining lecithin: Retinol acyltransferase (LRAT) activity
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Haaker, Maya W, Goossens, Vera, Hoogland, Nina A N, van Doorne, Hidde, Wang, Ziqiong, Jansen, Jeroen W A, Kaloyanova, Dora V, van de Lest, Chris H A, Houweling, Martin, Vaandrager, A Bas, Helms, J Bernd, Haaker, Maya W, Goossens, Vera, Hoogland, Nina A N, van Doorne, Hidde, Wang, Ziqiong, Jansen, Jeroen W A, Kaloyanova, Dora V, van de Lest, Chris H A, Houweling, Martin, Vaandrager, A Bas, and Helms, J Bernd
- Abstract
Lechthin:retinol acyltrasnferase (LRAT) is the main enzyme producing retinyl esters (REs) in quiescent hepatic stellate cells (HSCs). When cultured on stiff plastic culture plates, quiescent HSCs activate and lose their RE stores in a process similar to that in the liver following tissue damage, leading to fibrosis. Here we validated HSC cultures in soft gels to study RE metabolism in stable quiescent HSCs and investigated RE synthesis and breakdown in activating HSCs. HSCs cultured in a soft gel maintained characteristics of quiescent HSCs, including the size, amount and composition of their characteristic large lipid droplets. Quiescent gel-cultured HSCs maintained high expression levels of Lrat and a RE storing phenotype with low levels of RE breakdown. Newly formed REs are highly enriched in retinyl palmitate (RP), similar to freshly isolated quiescent HSCs, which is associated with high LRAT activity. Comparison of these quiescent gel-cultured HSCs with activated plastic-cultured HSCs showed that although during early activation the total RE levels and RP-enrichment are reduced, levels of RE formation are maintained and mediated by LRAT. Loss of REs was caused by enhanced RE breakdown in activating HSCs. Upon prolonged culturing, activated HSCs have lost their LRAT activity and produce small amounts of REs by DGAT1. This study reveals unexpected dynamics in RE metabolism during early HSC activation, which might be important in liver disease as early stages are reversible. Soft gel cultures provide a promising model to study RE metabolism in quiescent HSCs, allowing detailed molecular investigations on the mechanisms for storage and release.
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- 2024
8. The less conserved metal-binding site in human CRISP1 remains sensitive to zinc ions to permit protein oligomerization
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Sheng, Jie, Gadella, Bart M., Olrichs, Nick K., Kaloyanova, Dora V., and Helms, J. Bernd
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- 2021
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9. California Energy Systems for the 21st Century (CES-21) Program, CRADA TC02200 (Final Report)
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Helms, J., primary, Van Randwyk, J., additional, Taymaz, S., additional, Weed, J., additional, and McClelland, C., additional
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- 2022
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10. Combining Researchers' and Practitioners' Intelligences for STEM Improvement: A Study of the Local Labs of the Research and Practice Collaboratory. Executive Summary. Research+Practice Partnerships
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Inverness Research, Stokes, L., Carroll, B., Helms, J. V., Mitchell, H., Phillips, M., St. John, M., and Tambe, P.
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This is the executive summary of the report, "Combining Researchers' and Practitioners' Intelligences for STEM Improvement: A Study of the Local Labs of the Research and Practice Collaboratory." The Research and Practice Collaboratory (RPC) supported projects in three states--California, Maine, and Washington--that had the function of serving as "Local Labs" for the RPC. While these labs worked independently on specific, local STEM improvement problems, RPC leaders held themselves to shared conjectures (cite) about their work and its outcomes, convened multi-lab inquiry groups to probe cross-cutting issues, and met regularly to envision and plan RPC strategies and products. Each Local Lab was formed explicitly as a research-practice partnership (RPP), with dual purposes: (1) Address local problems of practice and producing usable, evidence-based knowledge for STEM improvement; and (2) Serve as existence-proofs of research-practice partnerships that thrive in a variety of institutional configurations. The Local Labs individually and the RPC as a whole also aimed to produce knowledge products--e.g., tools, slide decks, webinars, articles, and so on--that would contribute to the field, both to support STEM improvement and also to promote and support research-practice partnerships as an effective way to make research more valuable to the improvement of practice. This paper documents the formation of the Local Labs for the RPC and produced a detailed case study of each site. The case studies aim to portray distinctive features of the design and work of the Labs as research-practice partnerships, as well as the multiple benefits of the partnerships. It also looks across the cases to distill key findings and reflections. [For the full report, see ED598125.]
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- 2018
11. Chronic dietary changes in n-6/n-3 polyunsaturated fatty acid ratios cause developmental delay and reduce social interest in mice
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van Elst, Kim, Brouwers, Jos F., Merkens, Jessica E., Broekhoven, Mark H., Birtoli, Barbara, Helms, J. Bernd, and Kas, Martien J.H.
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- 2019
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12. Interpreting the lipidome: bioinformatic approaches to embrace the complexity
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Kyle, Jennifer E., Aimo, Lucila, Bridge, Alan J., Clair, Geremy, Fedorova, Maria, Helms, J. Bernd, Molenaar, Martijn R., Ni, Zhixu, Orešič, Matej, Slenter, Denise, Willighagen, Egon, and Webb-Robertson, Bobbie-Jo M.
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- 2021
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13. The Origins of Species-Specific Facial Morphology: The Proof Is in the Pigeon
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Helms, J. A.
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- 2007
14. Place de l’épuration extracorporelle de CO2 (ECCO2R) dans la prise en charge des pathologies respiratoires
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Diehl, J.L., Boisramé-Helms, J., Chardon-Couteau, A., Commereuc, M., Augy, J.-L., Sokoloff, A., Rivet, N., Gaussem, P., Smadja, D.M., and Aissaoui, N.
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- 2017
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15. Close Encounter With a Carolina Bay. Jones Lake State Park: An Environmental Education Learning Experience Designed for Grades 6-8.
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North Carolina State Dept. of Environment, Health, and Natural Resources, Raleigh. Div. of Parks and Recreation., Helms, J. Christopher, and Corbett, Robert J.
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This activity guide, developed to provide hands-on environmental education activities geared to Jones Lake State Park in North Carolina, is targeted for grades 6, 7, and 8 and meets curriculum objectives in the standard course of study established by the North Carolina Department of Public Instruction. Three types of activities are included: pre-visit, on-site, and post-visit. The on-site activity is conducted at the park, while pre- and post-visit activities are designed for the classroom. Major concepts included are: water quality, data collection, pH, animal adaptations, and geomorphology of a Carolina bay lake. Includes an introduction to the water cycle, a vocabulary list, scheduling worksheet, parental permission form, North Carolina Parks and Recreation program evaluation, sources of information on water resources, and information about Jones Lake State Park and Carolina bays. (MKR)
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- 1994
16. Wayne David Rasmussen: A Memorial (February 5, 1915 - April 30, 2004)
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Helms, J. Douglas
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- 2004
17. The Cellular and Molecular Origins of Beak Morphology
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Schneider, R. A. and Helms, J. A.
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- 2003
18. Inhibition of polyploidization in Pten-deficient livers reduces steatosis
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Moreno, Eva, Matondo, Augustine B, Bongiovanni, Laura, van de Lest, Chris H A, Molenaar, Martijn R, Toussaint, Mathilda J M, van Essen, Saskia C, Houweling, Martin, Helms, J Bernd, Westendorp, Bart, de Bruin, Alain, Cell Biology, Metabolism and Cancer, Pathobiologie, CS_Locomotion, Veterinaire biochemie, Equine Musculoskeletal Biology, Dep Biomolecular Health Sciences, Cell Biology, Metabolism and Cancer, Pathobiologie, CS_Locomotion, Veterinaire biochemie, Equine Musculoskeletal Biology, and Dep Biomolecular Health Sciences
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EXPRESSION ,PTEN ,GENES ,Hepatology ,Liver Neoplasms ,Peroxisome Proliferator-Activated Receptors ,PTEN Phosphohydrolase ,non-alcoholic fatty liver disease ,FATTY LIVER ,hepatocellular carcinoma ,polyploidization ,Lipids ,HEPATOCYTE PLOIDY ,atypical E2Fs ,Mice ,Phosphatidylinositol 3-Kinases ,FUSION ,Liver ,conditional knockout mice ,Hepatocytes ,steatosis ,Animals ,E2F8 ,Proto-Oncogene Proteins c-akt ,SUPPRESSION - Abstract
The tumour suppressor PTEN is a negative regulator of the PI3K/AKT signalling pathway. Liver-specific deletion of Pten in mice results in the hyper-activation PI3K/AKT signalling accompanied by enhanced genome duplication (polyploidization), marked lipid accumulation (steatosis) and formation of hepatocellular carcinomas. However, it is unknown whether polyploidization in this model has an impact on the development of steatosis and the progression towards liver cancer. Here, we used a liver-specific conditional knockout approach to delete Pten in combination with deletion of E2f7/8, known key inducers of polyploidization. As expected, Pten deletion caused severe steatosis and liver tumours accompanied by enhanced polyploidization. Additional deletion of E2f7/8 inhibited polyploidization, alleviated Pten-induced steatosis without affecting lipid species composition and accelerated liver tumour progression. Global transcriptomic analysis showed that inhibition of polyploidization in Pten-deficient livers resulted in reduced expression of genes involved in energy metabolism, including PPAR-gamma signalling. However, we find no evidence that deregulated genes in Pten-deficient livers are direct transcriptional targets of E2F7/8, supporting that reduction in steatosis and progression towards liver cancer are likely consequences of inhibiting polyploidization. Lastly, flow cytometry and image analysis on isolated primary wildtype mouse hepatocytes provided further support that polyploid cells can accumulate more lipid droplets than diploid hepatocytes. Collectively, we show that polyploidization promotes steatosis and function as an important barrier against liver tumour progression in Pten-deficient livers.
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- 2022
19. Evidence for Segregation of Sphingomyelin and Cholesterol during Formation of COPI-Coated Vesicles
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Brügger, Britta, Sandhoff, Roger, Wegehingel, Sabine, Gorgas, Karin, Malsam, Jörg, Helms, J. Bernd, Lehmann, Wolf-Dieter, Nickel, Walter, and Wieland, Felix T.
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- 2000
20. Receptor-Induced Polymerization of Coatomer
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Reinhard, Constanze, Harter, Cordula, Bremser, Martina, Brugger, Britta, Sohn, Kai, Helms, J. Bernd, and Wieland, Felix
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- 1999
21. Infections sévères à SARS-CoV-2
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Taghboulit, N., Voiriot, G., Demoule, A., and Helms, J.
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- 2021
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22. Blindness as an uncommon complication of Streptococcus pneumoniae systemic infection
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Clere-Jehl, R., Merdji, H., Derhy, D., and Helms, J.
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- 2019
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23. Acid Rain and Ozone Effects on Gas Exchange of Pinus ponderosa: A Comparison between Trees and Seedlings
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Momen, B., Anderson, P. D., Helms, J. A., and Houpis, J. L. J.
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- 1997
24. Characterization of acrosin and acrosin binding protein as novel CRISP2 interacting proteins in boar spermatozoa
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Zhang, Min, primary, Chiozzi, Riccardo Zenezini, additional, Bromfield, Elizabeth G, additional, Heck, Albert JR, additional, Helms, J Bernd, additional, and Gadella, Bart M, additional
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- 2023
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25. Hyperlipidemia elicits an atypical, Th1 like CD4+ T cell response: a key role for VLDL
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van Os, Bram W, primary, Vos, Winnie G, additional, Bosmans, Laura A, additional, van Tiel, Claudia M, additional, Lith, Sanne C, additional, den Toom, Myrthe, additional, Beckers, Linda, additional, Levels, Johannes H M, additional, van Wouw, Suzanne A E, additional, Zelcer, Noam, additional, Zaal, Esther A, additional, Berkers, Celia R, additional, van de Lest, Chris H A, additional, Helms, J Bernd, additional, Weber, Christian, additional, Atzler, Dorothee, additional, de Winther, Menno P J, additional, Baardman, Jeroen, additional, and Lutgens, Esther, additional
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- 2023
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26. Direct and GTP-Dependent Interaction of ADP Ribosylation Factor 1 with Coatomer Subunit β
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Zhao, Liyun, Helms, J. Bernd, Brugger, Britta, Harter, Cordula, Martoglio, Bruno, Graf, Roland, Brunner, Josef, and Wieland, Felix T.
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- 1997
27. A Major Transmembrane Protein of Golgi-Derived COPI-Coated Vesicles Involved in Coatomer Binding
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Sohn, Kai, Orci, Lelio, Ravazzola, Mariella, Amherdt, Mylène, Bremser, Martina, Lottspeich, Friedrich, Fiedler, Klaus, Helms, J. Bernd, and Wieland, Felix T.
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- 1996
28. National Soil Conservation Policies: A Historical Case Study of the Driftless Area
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Helms, J. Douglas, Pavelis, George A., Argabright, Scott, Cronshey, Roger G., and Sinclair, H. Raymond
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- 1996
29. Retinyl Ester Analysis by Orbitrap Mass Spectrometry
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Jansen, Jeroen W A, Haaker, Maya W, Zaal, Esther A, Helms, J Bernd, Jansen, Jeroen W A, Haaker, Maya W, Zaal, Esther A, and Helms, J Bernd
- Abstract
Retinoids are light-sensitive molecules that are normally detected by UV absorption techniques. Here we describe the identification and quantification of retinyl ester species by high-resolution mass spectrometry. Retinyl esters are extracted by the method of Bligh and Dyer and subsequently separated by HPLC in runs of 40 min. The retinyl esters are identified and quantified by mass spectrometry analysis. This procedure enables the highly sensitive detection and characterization of retinyl esters in biological samples such as hepatic stellate cells.
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- 2023
30. Characterization of acrosin and acrosin binding protein as novel CRISP2 interacting proteins in boar spermatozoa
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Zhang, Min, Chiozzi, Riccardo Zenezini, Bromfield, Elizabeth G, Heck, Albert Jr, Helms, J Bernd, Gadella, Bart M, Zhang, Min, Chiozzi, Riccardo Zenezini, Bromfield, Elizabeth G, Heck, Albert Jr, Helms, J Bernd, and Gadella, Bart M
- Abstract
Background: Previously, we reported that cysteine-rich secretory protein 2 is involved in high molecular weight complexes in boar spermatozoa. These cysteine-rich secretory protein 2protein complexes are formed at the last phase of sperm formation in the testis and play a role in sperm shaping and functioning. Objectives: This study aimed to identify cysteine-rich secretory protein 2 interacting partners. These binding partner interactions were investigated under different conditions, namely, non-capacitating conditions, after the induction of in vitro sperm capacitation and subsequently during an ionophore A23187-induced acrosome reaction. Materials and Methods: The incubated pig sperm samples were subjected to protein extraction. Extracted proteins were subjected to blue native gel electrophoresis and native immunoblots. Immunoreactive gel bands were excised and subjected to liquid chromatography–mass spectrometry (LC-MS) analysis for protein identification. Protein extracts were also subjected to CRISP2 immunoprecipitation and analyzed by LC-MS for protein identification. The most prominent cystein-rich secretory protein 2 interacting proteins that appeared in both independent LC-MS analyses were studied with a functional in situ proximity interaction assay to validate their property to interact with cystein-rich secretory protein 2 in pig sperm. Results: Blue native gel electrophoresis and native immunoblots revealed that cystein-rich secretory protein 2 was present within a ∼150 kDa protein complex under all three conditions. Interrogation of cystein-rich secretory-protein 2-immunoreactive bands from blue native gels as well as cystein-rich secretory protein 2 immunoprecipitated products using mass spectrometry consistently revealed that, beyond cystein-rich secretory protein 2, acrosin and acrosin binding protein were among the most abundant interacting proteins and did interact under all three conditions. Co-immunoprecipitation and immunoblotting indicated
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- 2023
31. Lipidomic profiling of rat hepatic stellate cells during activation reveals a two-stage process accompanied by increased levels of lysosomal lipids
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Molenaar, Martijn R, Haaker, Maya W, Vaandrager, A Bas, Houweling, Martin, Helms, J Bernd, Molenaar, Martijn R, Haaker, Maya W, Vaandrager, A Bas, Houweling, Martin, and Helms, J Bernd
- Abstract
Hepatic stellate cells (HSCs) are liver-resident cells best known for their role in vitamin A storage under physiological conditions. Upon liver injury, HSCs activate into myofibroblast-like cells, a key process in the onset of liver fibrosis. Lipids play an important role during HSC activation. Here, we provide a comprehensive characterization of the lipidomes of primary rat HSCs during 17 days of activation in vitro. For lipidomic data interpretation, we expanded our previously described Lipid Ontology (LION) and associated web application (LION/Web) with the LION-PCA heatmap module, which generates heatmaps of the most typical LION-signatures in lipidomic datasets. Furthermore, we used LION to perform pathway analysis to determine the significant metabolic conversions in lipid pathways. Together, we identify two distinct stages of HSC activation. In the first stage, we observe a decrease of saturated phosphatidylcholine, sphingomyelin, and phosphatidic acid and an increase in phosphatidylserine and polyunsaturated bis(monoacylglycero)phosphate (BMP), a lipid class typically localized at endosomes and lysosomes. In the second activation stage, BMPs, hexosylceramides, and ether-linked phosphatidylcholines are elevated, resembling a lysosomal lipid storage disease profile. The presence of isomeric structures of BMP in HSCs was confirmed ex vivo in MS-imaging datasets of steatosed liver sections. Finally, treatment with pharmaceuticals targeting the lysosomal integrity led to cell death in primary HSCs but not in HeLa cells. In summary, our combined data suggest that lysosomes play a critical role during a two-stage activation process of HSCs.
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- 2023
32. Thromboprophylaxis in critical care.
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Helms, J., Middeldorp, S., Spyropoulos, A.C., Helms, J., Middeldorp, S., and Spyropoulos, A.C.
- Abstract
01 januari 2023, Item does not contain fulltext
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- 2023
33. Retinyl Ester Analysis by Orbitrap Mass Spectrometry
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IOV MS, Cell Biology, Metabolism and Cancer, Veterinaire biochemie, Cell Biology, Metabolism & Cancer - Metabolomics, Jansen, Jeroen W A, Haaker, Maya W, Zaal, Esther A, Helms, J Bernd, IOV MS, Cell Biology, Metabolism and Cancer, Veterinaire biochemie, Cell Biology, Metabolism & Cancer - Metabolomics, Jansen, Jeroen W A, Haaker, Maya W, Zaal, Esther A, and Helms, J Bernd
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- 2023
34. Hyperlipidaemia elicits an atypical, T helper 1-like CD4 + T-cell response: a key role for very low-density lipoprotein.
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Veterinaire biochemie, Sub Biomol.Mass Spectrometry & Proteom., Cell Biology, Metabolism & Cancer - Metabolomics, van Os, Bram W, Vos, Winnie G, Bosmans, Laura A, van Tiel, Claudia M, Lith, Sanne C, den Toom, Myrthe S, Beckers, Linda, Levels, Johannes H M, van Wouw, Suzanne A E, Zelcer, Noam, Zaal, Esther A, Berkers, Celia R, van der Lest, Chris H A, Helms, J Bernd, Weber, Christian, Atzler, Dorothee, de Winther, Menno P J, Baardman, Jeroen, Lutgens, Esther, Veterinaire biochemie, Sub Biomol.Mass Spectrometry & Proteom., Cell Biology, Metabolism & Cancer - Metabolomics, van Os, Bram W, Vos, Winnie G, Bosmans, Laura A, van Tiel, Claudia M, Lith, Sanne C, den Toom, Myrthe S, Beckers, Linda, Levels, Johannes H M, van Wouw, Suzanne A E, Zelcer, Noam, Zaal, Esther A, Berkers, Celia R, van der Lest, Chris H A, Helms, J Bernd, Weber, Christian, Atzler, Dorothee, de Winther, Menno P J, Baardman, Jeroen, and Lutgens, Esther
- Published
- 2023
35. Lipidomic profiling of rat hepatic stellate cells during activation reveals a two-stage process accompanied by increased levels of lysosomal lipids
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Veterinaire biochemie, Cell Biology, Metabolism & Cancer - Metabolomics, Molenaar, Martijn R, Haaker, Maya W, Vaandrager, A Bas, Houweling, Martin, Helms, J Bernd, Veterinaire biochemie, Cell Biology, Metabolism & Cancer - Metabolomics, Molenaar, Martijn R, Haaker, Maya W, Vaandrager, A Bas, Houweling, Martin, and Helms, J Bernd
- Published
- 2023
36. Characterization of acrosin and acrosin binding protein as novel CRISP2 interacting proteins in boar spermatozoa
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Veterinaire biochemie, Sub Biomol.Mass Spectrometry & Proteom., Afd Biomol.Mass Spect. and Proteomics, FAH klinische reproductie, Cell Biology, Metabolism & Cancer - Metabolomics, FAH – Sustainable Ruminant Health, Zhang, Min, Chiozzi, Riccardo Zenezini, Bromfield, Elizabeth G, Heck, Albert Jr, Helms, J Bernd, Gadella, Bart M, Veterinaire biochemie, Sub Biomol.Mass Spectrometry & Proteom., Afd Biomol.Mass Spect. and Proteomics, FAH klinische reproductie, Cell Biology, Metabolism & Cancer - Metabolomics, FAH – Sustainable Ruminant Health, Zhang, Min, Chiozzi, Riccardo Zenezini, Bromfield, Elizabeth G, Heck, Albert Jr, Helms, J Bernd, and Gadella, Bart M
- Published
- 2023
37. Metal ions and redox balance regulate distinct amyloid-like aggregation pathways of GAPR-1
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Sheng, Jie, Olrichs, Nick K., Geerts, Willie J., Kaloyanova, Dora V., and Helms, J. Bernd
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- 2019
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38. Lipidomic profiling of rat hepatic stellate cells during activation reveals a two-stage process accompanied by increased levels of lysosomal lipids
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Molenaar, Martijn R., primary, Haaker, Maya W., additional, Vaandrager, A. Bas, additional, Houweling, Martin, additional, and Helms, J. Bernd, additional
- Published
- 2023
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39. Long-term (180-day) outcomes in critically ill patients with COVID-19 in the REMAP-CAP randomized clinical trial
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Florescu, S, Stanciu, D, Zaharia, M, Kosa, A, Codreanu, D, Kidwai, A, Masood, S, Kaye, C, Coutts, A, MacKay, L, Summers, C, Polgarova, P, Farahi, N, Fox, E, McWilliam, S, Hawcutt, D, Rad, L, O’Malley, L, Whitbread, J, Jones, D, Dore, R, Saunderson, P, Kelsall, O, Cowley, N, Wild, L, Thrush, J, Wood, H, Austin, K, Bélteczki, J, Magyar, I, Fazekas, Á, Kovács, S, Szőke, V, Donnelly, A, Kelly, M, Smyth, N, O’Kane, S, McClintock, D, Warnock, M, Campbell, R, McCallion, E, Azaiz, A, Charron, C, Godement, M, Geri, G, Vieillard-Baron, A, Johnson, P, McKenna, S, Hanley, J, Currie, A, Allen, B, McGoldrick, C, McMaster, M, Mani, A, Mathew, M, Kandeepan, R, Vignesh, C, TV, B, Ramakrishnan, N, James, A, Elvira, E, Jayakumar, D, Pratheema, R, Babu, S, Ebenezer, R, Krishnaoorthy, S, Ranganathan, L, Ganesan, M, Shree, M, Guilder, E, Butler, M, Cowdrey, K-A, Robertson, M, Ali, F, McMahon, E, Duffy, E, Chen, Y, Simmonds, C, McConnochie, R, O’Connor, C, El-Khawas, K, Richardson, A, Hill, D, Commons, R, Abdelkharim, H, Saxena, M, Muteithia, M, Dobell-Brown, K, Jha, R, Kalogirou, M, Ellis, C, Krishnamurthy, V, O’Connor, A, Thurairatnam, S, Mukherjee, D, Kaliappan, A, Vertue, M, Nicholson, A, Riches, J, Maloney, G, Kittridge, L, Solesbury, A, Ramos, A, Collins, D, Brickell, K, Reid, L, Smyth, M, Breen, P, Spain, S, Curley, G, McEvoy, N, Geoghegan, P, Clarke, J, Silversides, J, McGuigan, P, Ward, K, O’Neill, A, Finn, S, Wright, C, Green, J, Collins, É, Knott, C, Smith, J, Boschert, C, Slieker, K, Ewalds, E, Sanders, A, Wittenberg, W, Geurts, H, Poojara, L, Sara, T, Nand, K, Reeve, B, Dechert, W, Phillips, B, Oritz-Ruiz de Gordoa, L, Affleck, J, Shaikh, A, Murray, A, Ramanan, M, Frakking, T, Pinnell, J, Robinson, M, Gledhill, L, Wood, T, Sanghavi, R, Bhonagiri, D, Ford, M, Parikh, HG, Avard, B, Nourse, M, McDonald, B, Edmunds, N, Hoiting, O, Peters, M, Rengers, E, Evers, M, Prinssen, A, Morgan, M, Cole, J, Hill, H, Davies, M, Williams, A, Thomas, E, Davies, R, Wise, M, Grimm, P, Soukup, 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S, Wetherill, B, Brajković, A, Babel, J, Sever, H, Dragija, L, Kušan, I, Dushianthan, A, Cusack, R, De Courcy-Golder, K, Salmon, K, Burnish, R, Smith, S, Ruiz, W, Duke, Z, Johns, M, Male, M, Gladas, K, Virdee, S, Swabe, J, Tomlinson, H, Rohde, G, Grünewaldt, A, Bojunga, J, Petros, S, Kunz, K, Schütze, B, Weismann, D, Frey, A, Drayss, M, Goebeler, ME, Flor, T, Fragner, G, Wahl, N, Totzke, J, Sayehli, C, Hakak, S, Altaf, W, O'Sullivan, M, Murphy, A, Walsh, L, Rega La Valle, A, Bewley, J, Sweet, K, Grimmer, L, Johnson, R, Wyatt, R, Morgan, K, Varghese, S, Willis, J, Stratton, E, Kyle, L, Putensen, D, Drury, K, Skorko, A, Bremmer, P, Ward, G, Bassford, C, Sligl, W, Baig, N, Rewa, O, Bagshaw, S, Basile, K, Stavor, D, Burbee, D, McNamara, A, Wunderley, R, Bensen, N, Adams, P, Vita, T, Buhay, M, Scholl, D, Gilliam, M, Winters, J, Doherty, K, Berryman, E, Ghaffari, M, Marroquin, O, Quinn, K, Garrard, W, Kalchthaler, K, Beard, G, Skrtich, A, Bagavathy, K, Drapola, D, Bryan-Morris, K, Arnold, J, Reynolds, B, Hussain, M, Dunsavage, J, Saiyed, S, Hernandez, E, Goldman, J, Brown, C, Comp, S, Raczek, J, Morris, J, Vargas Jr., J, Weiss, D, Hensley, J, Kochert, E, Wnuk, C, Nemeth, C, Mowery, B, Hutchinson, C, Winters, L, McAdams, D, Walker, G, Minnier, T, Wisniewski, M, Mayak, K, McCreary, E, Bariola, R, Viehman, A, Daley, J, Lopus, A, Schmidhofer, M, Ambrosino, R, Keen, S, Toffalo, S, Stambaugh, M, Trimmer, K, Perri, R, Casali, S, Medva, R, Massar, B, Beyerl, A, Burkey, J, Keeler, S, Lowery, M, Oncea, L, Daugherty, J, Sevilla, C, Woelke, A, Dice, J, Weber, L, Roth, J, Ferringer, C, Beer, D, Fesz, J, Carpio, L, Colin, G, Zinzoni, V, Maquigneau, N, Henri-Lagarrigue, M, Pouplet, C, Reill, L, Distler, M, Maselli, A, Martynoga, R, Trask, K, Butler, A, Attwood, B, Parsons, P, Campbell, B, Smith, A, Page, V, Zhao, X, Oza, D, Abrahamson, G, Sheath, B, Young, P, Young, C, Lesona, E, Navarra, L, Cruz, R, Delaney, K, Aguilar-Dano, A, Gojanovic, M, Rhodes, J, Anderson, T, Morris, S, Nayyar, V, Bowen, D, Kong, J, Joy, J, Fuchs, R, Lambert, B, Tai, C, Thomas, A, Keen, A, Tierney, C, Omer, N, Bacon, G, Tridente, A, Shuker, K, Anders, J, Greer, S, Scott, P, Millington, A, Buchanan, P, Binnie, A, Powell, E, McMillan, A, Luk, T, Aref, N, Denmade, C, Sadera, G, Jacob, R, Hughes, D, Sterba, M, Geng, W, Digby, S, Southern, D, Reddy, H, Hulse, S, Campbell, A, Garton, M, Watkins, C, Smuts, S, Quinn, A, Simpson, B, McMillan, C, Finch, C, Hill, C, Cooper, J, Budd, J, Small, C, O’Leary, R, Collins, E, Holland, A, Alexander, P, Felton, T, Ferguson, S, Sellers, K, Ward, L, Yates, D, Birkinshaw, I, Kell, K, Scott, Z, Pearson, H, Hashmi, M, Hassan, N, Panjwani, A, Umrani, Z, Shaikh, M, Ain, Q, Kanwal, D, Van Bree, S, Bouw-Ruiter, M, Osinga, M, Van Zanten, A, McEldrew, R, Rashan, S, Singh, V, Azergui, N, Bari, S, Beltran, M, Brugman, C, Groeneveld, E, Jafarzadeh, M, Keijzer-Timmers, N, Kester, E, Koelink, M, Kwakkenbos-Craanen, M, Okundaye, C, Parker, L, Peters, S, Post, S, Rietveld, I, Scheepstra-Beukers, I, Schreuder, G, Smit, A, Brillinger, N, Markgraf, R, Eichinger, F, Doran, P, Anjum, A, Best-Lane, J, Barton, F, Miller, L, Richards-Belle, A, Saull, M, Sprinckmoller, S, Wiley, D, Darnell, R, Au, C, Lindstrum, K, Cheng, A, Forbes, A, Heritier, S, Trapani, T, Cuthbertson, B, Manoharan, V, Dondrop, A, Tolppa, T, Ehrmann, S, Hullegie, S, Povoa, P, Beasley, R, Daneman, N, McGloughlin, S, Paterson, D, Venkatesh, B, De Jong, M, Uyeki, T, Baillie, K, Netea, M, Orr, K, Patanwala, A, Tong, S, Cooper, N, Galea, J, Leavis, H, Ogungbenro, K, Patawala, A, Rademaker, E, Youngstein, T, Carrier, M, Fergusson, D, Hunt, B, Kumar, A, Laffan, M, Lother, S, Middeldorp, S, Stanworth, S, De Man, A, Masse, M-H, Abraham, J, Arnold, D, Begin, P, Charlewood, R, Chasse, M, Coyne, M, Daly, J, Gosbell, I, Harvala-Simmonds, H, MacLennan, S, McDyer, J, Menon, D, Pridee, N, Roberts, D, Thomas, H, Tinmouth, A, Triulzi, D, Walsh, T, Wood, E, Calfee, C, O’Kane, C, Shyamsundar, M, Sinha, P, Thompson, T, Young, I, Burrell, A, Ferguson, N, Hodgson, C, Orford, N, Phua, J, Baron, R, Epelman, S, Frankfurter, C, Gommans, F, Kim, E, Leaf, D, Vaduganathan, M, Van Kimmenade, R, Sanil, A, Van Beurden, M, Effelaar, E, Schotsman, J, Boyd, C, Harland, C, Shearer, A, Wren, J, Attanayaka, U, Darshana, S, Ishani, P, Udayanga, I, Higgins, AM, Berry, LR, Lorenzi, E, Murthy, S, McQuilten, Z, Mouncey, PR, Al-Beidh, F, Annane, D, Arabi, YM, Beane, A, Van Bentum-Puijk, W, Bhimani, Z, Bonten, MJM, Bradbury, CA, Brunkhorst, FM, Buzgau, A, Buxton, M, Charles, WN, Cove, M, Detry, MA, Estcourt, LJ, Fagbodun, EO, Fitzgerald, M, Girard, TD, Goligher, EC, Goossens, H, Haniffa, R, Hills, T, Horvat, CM, Huang, DT, Ichihara, N, Lamontagne, F, Marshall, JC, McAuley, DF, McGlothlin, A, McGuinness, SP, McVerry, BJ, Neal, MD, Nichol, AD, Parke, RL, Parker, JC, Parry-Billings, K, Peters, SEC, Reyes, LF, Rowan, KM, Saito, H, Santos, MS, Saunders, CT, Serpa-Neto, A, Seymour, CW, Shankar-Hari, M, Stronach, LM, Turgeon, AF, Turner, AM, Van de Veerdonk, FL, Zarychanski, R, Green, C, Lewis, RJ, Angus, DC, McArthur, CJ, Berry, S, Derde, LPG, Gordon, AC, Webb, SA, Lawler, PR, Comm REMAP-CAP Investigators, Apollo - University of Cambridge Repository, Intensive Care Medicine, Université de Versailles Saint-Quentin-en-Yvelines - UFR Sciences de la santé Simone Veil (UVSQ Santé), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Hôpital Raymond Poincaré [Garches], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Pittsburgh Foundation, PF, Amgen, Health Research Board, HRB: CTN 2014-012, Horizon 2020 Framework Programme, H2020: 101003589, Translational Breast Cancer Research Consortium, TBCRC, Canadian Institutes of Health Research, IRSC: 158584, Heart and Stroke Foundation of Canada, HSF, National Institute for Health and Care Research, NIHR, European Commission, EC, National Health and Medical Research Council, NHMRC: 1101719, APP194811, CS-2016-16-011, GNT2008447, RP-2015-06-18, Office of Health and Medical Research, OHMR, Health Research Council of New Zealand, HRC: 16/631, Eisai, Ministère des Affaires Sociales et de la Santé: PHRC-20-0147, Université Pierre et Marie Curie, UPMC, NIHR Imperial Biomedical Research Centre, BRC, Minderoo Foundation, Funding/Support : The Platform for European Preparedness Against (Re-) emerging Epidemics (PREPARE) consortium by the European Union, FP7-HEALTH-2013-INNOVATION-1 (#602525), the Rapid European COVID-19 Emergency Research response (RECOVER) consortium by the European Union’s Horizon 2020 research and innovation programme (#101003589), the Australian National Health and Medical Research Council (#APP1101719), the Australian Medical Research Future Fund (#APP2002132), the Health Research Council of New Zealand (#16/631), the Canadian Institutes of Health Research Strategy for Patient-Oriented Research Innovative Clinical Trials Program Grant (#158584) and the Canadian Institute of Health Research COVID-19 Rapid Research Funding (#447335), the UK National Institute for Health Research (NIHR) and the NIHR Imperial Biomedical Research Centre, the Health Research Board of Ireland (CTN 2014-012), the UPMC Learning While Doing Program, the Translational Breast Cancer Research Consortium, the French Ministry of Health (PHRC-20-0147), the Wellcome Trust Innovations Project (215522), the Minderoo Foundation, the EU Programme Emergency Support Instrument, the NHS Blood and Transplant Research and Development Programme, the Translational Breast Cancer Research Consortium, the NSW Office of Health and Medical Research, Amgen, Eisai, and the Pittsburgh Foundation. Dr Higgins is funded by an NHMRC Emerging Leadership Fellowship (GNT2008447). Dr McQuilten is funded by an NHMRC Emerging Leadership Fellowship (APP194811). Dr Gordon is funded by an NIHR Research Professorship (RP-2015-06-18) and Dr Shankar-Hari by an NIHR Clinician Scientist Fellowship (CS-2016-16-011). Dr Turgeon is the Chairholder of the Canada Research Chair in Critical Care Neurology and Trauma. Dr Lawler is supported by a career award from the Heart and Stroke Foundation of Canada., and European Project: 602525,EC:FP7:HEALTH,FP7-HEALTH-2013-INNOVATION-1,PREPARE(2014)
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Adult ,Male ,corticosteroid ,[SDV]Life Sciences [q-bio] ,Critical Illness ,lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4] ,antiplatelet ,Lopinavir ,Adaptive platform trial randomized controlled trial intensive care, pneumonia COVID-19 antiplatelet immunoglobulin antiviral corticosteroid immune modulation anticoagulation ,All institutes and research themes of the Radboud University Medical Center ,Adrenal Cortex Hormones ,Humans ,anticoagulation ,intensive care, pneumonia ,COVID-19 Serotherapy ,Original Investigation ,Medicine(all) ,immune modulation ,Ritonavir ,SARS-CoV-2 ,COVID-19 ,Anticoagulants ,Bayes Theorem ,General Medicine ,Middle Aged ,antiviral ,Receptors, Interleukin-6 ,Adaptive platform trial ,randomized controlled trial ,Female ,Human medicine ,immunoglobulin ,Follow-Up Studies ,Hydroxychloroquine - Abstract
ImportanceThe longer-term effects of therapies for the treatment of critically ill patients with COVID-19 are unknown.ObjectiveTo determine the effect of multiple interventions for critically ill adults with COVID-19 on longer-term outcomes.Design, Setting, and ParticipantsPrespecified secondary analysis of an ongoing adaptive platform trial (REMAP-CAP) testing interventions within multiple therapeutic domains in which 4869 critically ill adult patients with COVID-19 were enrolled between March 9, 2020, and June 22, 2021, from 197 sites in 14 countries. The final 180-day follow-up was completed on March 2, 2022.InterventionsPatients were randomized to receive 1 or more interventions within 6 treatment domains: immune modulators (n = 2274), convalescent plasma (n = 2011), antiplatelet therapy (n = 1557), anticoagulation (n = 1033), antivirals (n = 726), and corticosteroids (n = 401).Main Outcomes and MeasuresThe main outcome was survival through day 180, analyzed using a bayesian piecewise exponential model. A hazard ratio (HR) less than 1 represented improved survival (superiority), while an HR greater than 1 represented worsened survival (harm); futility was represented by a relative improvement less than 20% in outcome, shown by an HR greater than 0.83.ResultsAmong 4869 randomized patients (mean age, 59.3 years; 1537 [32.1%] women), 4107 (84.3%) had known vital status and 2590 (63.1%) were alive at day 180. IL-6 receptor antagonists had a greater than 99.9% probability of improving 6-month survival (adjusted HR, 0.74 [95% credible interval {CrI}, 0.61-0.90]) and antiplatelet agents had a 95% probability of improving 6-month survival (adjusted HR, 0.85 [95% CrI, 0.71-1.03]) compared with the control, while the probability of trial-defined statistical futility (HR >0.83) was high for therapeutic anticoagulation (99.9%; HR, 1.13 [95% CrI, 0.93-1.42]), convalescent plasma (99.2%; HR, 0.99 [95% CrI, 0.86-1.14]), and lopinavir-ritonavir (96.6%; HR, 1.06 [95% CrI, 0.82-1.38]) and the probabilities of harm from hydroxychloroquine (96.9%; HR, 1.51 [95% CrI, 0.98-2.29]) and the combination of lopinavir-ritonavir and hydroxychloroquine (96.8%; HR, 1.61 [95% CrI, 0.97-2.67]) were high. The corticosteroid domain was stopped early prior to reaching a predefined statistical trigger; there was a 57.1% to 61.6% probability of improving 6-month survival across varying hydrocortisone dosing strategies.Conclusions and RelevanceAmong critically ill patients with COVID-19 randomized to receive 1 or more therapeutic interventions, treatment with an IL-6 receptor antagonist had a greater than 99.9% probability of improved 180-day mortality compared with patients randomized to the control, and treatment with an antiplatelet had a 95.0% probability of improved 180-day mortality compared with patients randomized to the control. Overall, when considered with previously reported short-term results, the findings indicate that initial in-hospital treatment effects were consistent for most therapies through 6 months.
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- 2023
40. The fate of porcine sperm CRISP2 from the perinuclear theca before and after in vitro fertilization
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Zhang, M, Bromfield, E G, Helms, J B, Gadella, B M, Veterinaire biochemie, and FAH klinische reproductie
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Male ,Swine ,Acrosome Reaction ,Cell Biology ,General Medicine ,Fertilization in Vitro ,perinuclear theca ,porcine ,decondensation ,Spermatozoa ,sperm ,Reproductive Medicine ,Semen ,fertilization ,CRISP2 ,Animals ,Cysteine ,oocyte - Abstract
In a previous study, we reported that porcine sperm cysteine-rich secretory protein 2 (CRISP2) is localized in the post-acrosomal sheath-perinuclear theca (PT) as reduction-sensitive oligomers. In the current study, the decondensation and removal of CRISP2 was investigated during in vitro sperm capacitation, after both the induction of the acrosome reaction and in vitro fertilization. Confocal immunofluorescent imaging revealed that additional CRISP2 fluorescence appeared on the apical ridge and on the equatorial segment (EqS) of the sperm head following capacitation, likely due to cholesterol removal. After an ionophore A23187-induced acrosome reaction, CRISP2 immunofluorescence disappeared from the apical ridge and the EqS area partly not only owing to the removal of the acrosomal shroud vesicles, but to its presence in a subdomain of EqS. The fate of sperm head CRISP2 was further examined post-fertilization. In vitro matured porcine oocytes were co-incubated with boar sperm cells for 6–8 h and the zygotes were processed for CRISP2 immunofluorescent staining. Notably, decondensation of CRISP2, and thus of the sperm PT, occurred while the sperm nucleus was still fully condensed. CRISP2 was no longer detectable in fertilized oocytes in which sperm nuclear decondensation and paternal pronucleus formation were apparent. This rapid dispersal of CRISP2 in the PT is likely regulated by redox reactions for which its cysteine-rich domain is sensitive. Reduction of disulfide bridges within CRISP2 oligomers may be instrumental for PT dispersal and elimination.
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- 2022
41. Climate, geography, and the mating phenology of ants
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Helms, J. A., primary
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- 2022
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42. Normothermic Ex Vivo Liver Platform Using Porcine Slaughterhouse Livers for Disease Modeling
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Krüger, Melanie, primary, Ruppelt, Alicia, additional, Kappler, Benjamin, additional, Van Soest, Elke, additional, Samsom, Roos Anne, additional, Grinwis, Guy C. M., additional, Geijsen, Niels, additional, Helms, J. Bernd, additional, Stijnen, Marco, additional, Kock, Linda M., additional, Rasponi, Marco, additional, Kooistra, Hans S., additional, and Spee, Bart, additional
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- 2022
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43. Lipid analysis of Eimeria sporozoites reveals exclusive phospholipids, a phylogenetic mosaic of endogenous synthesis, and a host-independent lifestyle
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Kong, Pengfei, Lehmann, Maik J., Helms, J. Bernd, Brouwers, Jos F., and Gupta, Nishith
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- 2018
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44. Ultrastructure and lipid composition of detergent-resistant membranes derived from mammalian sperm and two types of epithelial cells
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van Gestel, Renske A., Brouwers, Jos F., Ultee, Anton, Helms, J. Bernd, and Gadella, Bart M.
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- 2016
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45. Funktionserhaltende Chirurgie des Akustikusneurinoms — Ergebnisse interdisziplinärer Kooperation
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Behr, R., Schlake, H. P., Michel, O., Wedekind, C., Helms, J., Stennert, E., Roosen, K., Klug, N., Bootz, F., and Strauss, G.
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- 2002
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46. Normothermic Ex Vivo Liver Platform Using Porcine Slaughterhouse Livers for Disease Modeling
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Krüger, Melanie, Ruppelt, Alicia, Kappler, Benjamin, Van Soest, Elke, Samsom, Roos Anne, Grinwis, Guy C M, Geijsen, Niels, Helms, J Bernd, Stijnen, Marco, Kock, Linda M, Rasponi, Marco, Kooistra, Hans S, Spee, Bart, Krüger, Melanie, Ruppelt, Alicia, Kappler, Benjamin, Van Soest, Elke, Samsom, Roos Anne, Grinwis, Guy C M, Geijsen, Niels, Helms, J Bernd, Stijnen, Marco, Kock, Linda M, Rasponi, Marco, Kooistra, Hans S, and Spee, Bart
- Abstract
Metabolic and toxic liver disorders, such as fatty liver disease (steatosis) and drug-induced liver injury, are highly prevalent and potentially life-threatening. To allow for the study of these disorders from the early stages onward, without using experimental animals, we collected porcine livers in a slaughterhouse and perfused these livers normothermically. With our simplified protocol, the perfused slaughterhouse livers remained viable and functional over five hours of perfusion, as shown by hemodynamics, bile production, indocyanine green clearance, ammonia metabolism, gene expression and histology. As a proof-of-concept to study liver disorders, we show that an infusion of free fatty acids and acetaminophen results in early biochemical signs of liver damage, including reduced functionality. In conclusion, the present platform offers an accessible system to perform research in a functional, relevant large animal model while avoiding using experimental animals. With further improvements to the model, prolonged exposure could make this model a versatile tool for studying liver diseases and potential treatments.
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- 2022
47. High Resolution Proteomic Analysis of Subcellular Fractionated Boar Spermatozoa Provides Comprehensive Insights Into Perinuclear Theca-Residing Proteins
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Veterinaire biochemie, Sub Biomol.Mass Spectrometry & Proteom., Afd Biomol.Mass Spect. and Proteomics, dB&C FR-RMSC FR, dB&C FR-RMSC RMSC, FAH klinische reproductie, dES/dFAH FR, Zhang, Min, Chiozzi, Riccardo Zenezini, Skerrett-Byrne, David A, Veenendaal, Tineke, University, University Medical Center Utrecht, Heck, Albert J R, Nixon, Brett, Helms, J Bernd, Gadella, Bart M, Bromfield, Elizabeth G, Veterinaire biochemie, Sub Biomol.Mass Spectrometry & Proteom., Afd Biomol.Mass Spect. and Proteomics, dB&C FR-RMSC FR, dB&C FR-RMSC RMSC, FAH klinische reproductie, dES/dFAH FR, Zhang, Min, Chiozzi, Riccardo Zenezini, Skerrett-Byrne, David A, Veenendaal, Tineke, University, University Medical Center Utrecht, Heck, Albert J R, Nixon, Brett, Helms, J Bernd, Gadella, Bart M, and Bromfield, Elizabeth G
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- 2022
48. The fate of porcine sperm CRISP2 from the perinuclear theca before and after in vitro fertilization
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Veterinaire biochemie, FAH klinische reproductie, Zhang, M, Bromfield, E G, Helms, J B, Gadella, B M, Veterinaire biochemie, FAH klinische reproductie, Zhang, M, Bromfield, E G, Helms, J B, and Gadella, B M
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- 2022
49. Inhibition of polyploidization in Pten-deficient livers reduces steatosis
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Cell Biology, Metabolism and Cancer, Pathobiologie, CS_Locomotion, Veterinaire biochemie, Equine Musculoskeletal Biology, Dep Biomolecular Health Sciences, Moreno, Eva, Matondo, Augustine B, Bongiovanni, Laura, van de Lest, Chris H A, Molenaar, Martijn R, Toussaint, Mathilda J M, van Essen, Saskia C, Houweling, Martin, Helms, J Bernd, Westendorp, Bart, de Bruin, Alain, Cell Biology, Metabolism and Cancer, Pathobiologie, CS_Locomotion, Veterinaire biochemie, Equine Musculoskeletal Biology, Dep Biomolecular Health Sciences, Moreno, Eva, Matondo, Augustine B, Bongiovanni, Laura, van de Lest, Chris H A, Molenaar, Martijn R, Toussaint, Mathilda J M, van Essen, Saskia C, Houweling, Martin, Helms, J Bernd, Westendorp, Bart, and de Bruin, Alain
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- 2022
50. Normothermic Ex Vivo Liver Platform Using Porcine Slaughterhouse Livers for Disease Modeling
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Interne geneeskunde GD, VPDC pathologie, dPB CR, VP pathologie, Veterinaire biochemie, CS_Welfare & emerging diseases, CS_STEM, Krüger, Melanie, Ruppelt, Alicia, Kappler, Benjamin, Van Soest, Elke, Samsom, Roos Anne, Grinwis, Guy C M, Geijsen, Niels, Helms, J Bernd, Stijnen, Marco, Kock, Linda M, Rasponi, Marco, Kooistra, Hans S, Spee, Bart, Interne geneeskunde GD, VPDC pathologie, dPB CR, VP pathologie, Veterinaire biochemie, CS_Welfare & emerging diseases, CS_STEM, Krüger, Melanie, Ruppelt, Alicia, Kappler, Benjamin, Van Soest, Elke, Samsom, Roos Anne, Grinwis, Guy C M, Geijsen, Niels, Helms, J Bernd, Stijnen, Marco, Kock, Linda M, Rasponi, Marco, Kooistra, Hans S, and Spee, Bart
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- 2022
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