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860 results on '"Hellman, Ulf"'

1. Barley as a green factory for the production of functional Flt3 ligand.

Author

Erlendsson, Lýdur S, Muench, Marcus O, Hellman, Ulf, Hrafnkelsdóttir, Soffía M, Jonsson, Anders, Balmer, Yves, Mäntylä, Einar, and Orvar, Björn L

Subjects
Bioresource and Agricultural Engineering, Medical Biotechnology, Other Plant Sciences, Cell Growth Processes, Cell Line, Tumor, Cloning, Molecular, Flow Cytometry, Hordeum, Humans, Membrane Proteins, Plants, Genetically Modified, Recombinant Proteins
Abstract

Biologically active recombinant human Flt3 ligand was expressed and isolated from transgenic barley seeds. Its expression is controlled by a tissue specific promoter that confines accumulation of the recombinant protein to the endosperm tissue of the seed. The recombinant Flt3 ligand variant expressed in the seeds contains an HQ-tag for affinity purification on immobilized metal ion affinity chromatography (IMAC) resin. The tagged protein was purified from seed extracts to near homogeneity using sequential chromatography on IMAC affinity resin and cation exchange resin. We also show that the recombinant Flt3 ligand protein undergoes posttranslational modifications: it is a glycoprotein containing alpha-1,3-fucose and alpha-1,2-xylose. The HQ-tagged Flt3 ligand variant exhibits comparable biological activity to commercial Flt3 ligand. This is the first report showing expression and accumulation of recombinant human growth factor in barley seeds with a yield of active protein similar to a bacterial expression system. The present results demonstrate that plant molecular farming is a viable approach for the bioproduction of human-derived growth factors.

Published
2010

6. Data from RNA Helicase A Is a Downstream Mediator of KIF1Bβ Tumor-Suppressor Function in Neuroblastoma

Author

Chen, Zhi Xiong, primary, Wallis, Karin, primary, Fell, Stuart M., primary, Sobrado, Veronica R., primary, Hemmer, Marie C., primary, Ramsköld, Daniel, primary, Hellman, Ulf, primary, Sandberg, Rickard, primary, Kenchappa, Rajappa S., primary, Martinson, Tommy, primary, Johnsen, John I., primary, Kogner, Per, primary, and Schlisio, Susanne, primary

Published
2023
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7. Supplementary Figure 6 from RNA Helicase A Is a Downstream Mediator of KIF1Bβ Tumor-Suppressor Function in Neuroblastoma

Author

Chen, Zhi Xiong, primary, Wallis, Karin, primary, Fell, Stuart M., primary, Sobrado, Veronica R., primary, Hemmer, Marie C., primary, Ramsköld, Daniel, primary, Hellman, Ulf, primary, Sandberg, Rickard, primary, Kenchappa, Rajappa S., primary, Martinson, Tommy, primary, Johnsen, John I., primary, Kogner, Per, primary, and Schlisio, Susanne, primary

Published
2023
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8. Supplementary Figure 4 from RNA Helicase A Is a Downstream Mediator of KIF1Bβ Tumor-Suppressor Function in Neuroblastoma

Author

Chen, Zhi Xiong, primary, Wallis, Karin, primary, Fell, Stuart M., primary, Sobrado, Veronica R., primary, Hemmer, Marie C., primary, Ramsköld, Daniel, primary, Hellman, Ulf, primary, Sandberg, Rickard, primary, Kenchappa, Rajappa S., primary, Martinson, Tommy, primary, Johnsen, John I., primary, Kogner, Per, primary, and Schlisio, Susanne, primary

Published
2023
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9. Supplementary Table S3 from RNA Helicase A Is a Downstream Mediator of KIF1Bβ Tumor-Suppressor Function in Neuroblastoma

Author

Chen, Zhi Xiong, primary, Wallis, Karin, primary, Fell, Stuart M., primary, Sobrado, Veronica R., primary, Hemmer, Marie C., primary, Ramsköld, Daniel, primary, Hellman, Ulf, primary, Sandberg, Rickard, primary, Kenchappa, Rajappa S., primary, Martinson, Tommy, primary, Johnsen, John I., primary, Kogner, Per, primary, and Schlisio, Susanne, primary

Published
2023
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10. Supplementary Figure 7 from RNA Helicase A Is a Downstream Mediator of KIF1Bβ Tumor-Suppressor Function in Neuroblastoma

Author

Chen, Zhi Xiong, primary, Wallis, Karin, primary, Fell, Stuart M., primary, Sobrado, Veronica R., primary, Hemmer, Marie C., primary, Ramsköld, Daniel, primary, Hellman, Ulf, primary, Sandberg, Rickard, primary, Kenchappa, Rajappa S., primary, Martinson, Tommy, primary, Johnsen, John I., primary, Kogner, Per, primary, and Schlisio, Susanne, primary

Published
2023
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11. Supplementary Methods from RNA Helicase A Is a Downstream Mediator of KIF1Bβ Tumor-Suppressor Function in Neuroblastoma

Author

Chen, Zhi Xiong, primary, Wallis, Karin, primary, Fell, Stuart M., primary, Sobrado, Veronica R., primary, Hemmer, Marie C., primary, Ramsköld, Daniel, primary, Hellman, Ulf, primary, Sandberg, Rickard, primary, Kenchappa, Rajappa S., primary, Martinson, Tommy, primary, Johnsen, John I., primary, Kogner, Per, primary, and Schlisio, Susanne, primary

Published
2023
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12. Supplementary Figure Legends from RNA Helicase A Is a Downstream Mediator of KIF1Bβ Tumor-Suppressor Function in Neuroblastoma

Author

Chen, Zhi Xiong, primary, Wallis, Karin, primary, Fell, Stuart M., primary, Sobrado, Veronica R., primary, Hemmer, Marie C., primary, Ramsköld, Daniel, primary, Hellman, Ulf, primary, Sandberg, Rickard, primary, Kenchappa, Rajappa S., primary, Martinson, Tommy, primary, Johnsen, John I., primary, Kogner, Per, primary, and Schlisio, Susanne, primary

Published
2023
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13. Supplementary Figure 3 from RNA Helicase A Is a Downstream Mediator of KIF1Bβ Tumor-Suppressor Function in Neuroblastoma

Author

Chen, Zhi Xiong, primary, Wallis, Karin, primary, Fell, Stuart M., primary, Sobrado, Veronica R., primary, Hemmer, Marie C., primary, Ramsköld, Daniel, primary, Hellman, Ulf, primary, Sandberg, Rickard, primary, Kenchappa, Rajappa S., primary, Martinson, Tommy, primary, Johnsen, John I., primary, Kogner, Per, primary, and Schlisio, Susanne, primary

Published
2023
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14. Supplementary Figure 1 from RNA Helicase A Is a Downstream Mediator of KIF1Bβ Tumor-Suppressor Function in Neuroblastoma

Author

Chen, Zhi Xiong, primary, Wallis, Karin, primary, Fell, Stuart M., primary, Sobrado, Veronica R., primary, Hemmer, Marie C., primary, Ramsköld, Daniel, primary, Hellman, Ulf, primary, Sandberg, Rickard, primary, Kenchappa, Rajappa S., primary, Martinson, Tommy, primary, Johnsen, John I., primary, Kogner, Per, primary, and Schlisio, Susanne, primary

Published
2023
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15. Supplementary Figure 5 from RNA Helicase A Is a Downstream Mediator of KIF1Bβ Tumor-Suppressor Function in Neuroblastoma

Author

Chen, Zhi Xiong, primary, Wallis, Karin, primary, Fell, Stuart M., primary, Sobrado, Veronica R., primary, Hemmer, Marie C., primary, Ramsköld, Daniel, primary, Hellman, Ulf, primary, Sandberg, Rickard, primary, Kenchappa, Rajappa S., primary, Martinson, Tommy, primary, Johnsen, John I., primary, Kogner, Per, primary, and Schlisio, Susanne, primary

Published
2023
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16. Supplementary Figure 2 from RNA Helicase A Is a Downstream Mediator of KIF1Bβ Tumor-Suppressor Function in Neuroblastoma

Author

Chen, Zhi Xiong, primary, Wallis, Karin, primary, Fell, Stuart M., primary, Sobrado, Veronica R., primary, Hemmer, Marie C., primary, Ramsköld, Daniel, primary, Hellman, Ulf, primary, Sandberg, Rickard, primary, Kenchappa, Rajappa S., primary, Martinson, Tommy, primary, Johnsen, John I., primary, Kogner, Per, primary, and Schlisio, Susanne, primary

Published
2023
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26. Pancreatic Islet Amyloid in the Degu Is Derived from Insulin

Author

O’Brien, Timothy D., Hellman, Ulf, Westermark, Per, Wernstedt, Christer, Rathbun, William B., Johnson, Kenneth H., Natvig, Jacob B., editor, Førre, Øystein, editor, Husby, Gunnar, editor, Husebekk, Anne, editor, Skogen, Bjørn, editor, Sletten, Knut, editor, and Westermark, Per, editor

Published
1991
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30. Protein profiling of genomic instability in endometrial cancer

Author

Gemoll, Timo, Habermann, Jens K., Lahmann, Johanna, Szymczak, Silke, Lundgren, Caroline, Bündgen, Nana K., Jungbluth, Thomas, Nordström, Britta, Becker, Susanne, Lomnytska, Marta I., Bruch, Hans-Peter, Ziegler, Andreas, Hellman, Ulf, Auer, Gert, Roblick, Uwe J., and Jörnvall, Hans

Published
2012
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40. Preferential oxidation of the second phosphatase domain of receptor-like PTP-[alpha] revealed by an antibody against oxidized protein tyrosine phosphatases

Author

Persson, Camilla, Sjoblom, Tobias, Groen, Arnoud, Kappert, Kai, Engstrom, Ulla, Hellman, Ulf, Heldin, Carl-Henrik, den Hertog, Jeroen, and Ostman, Arne

Subjects
Phosphatases -- Research, Science and technology
Abstract

Protein tyrosine phosphatases (PTPs) constitute a large enzyme family with important biological functions. Inhibition of PTP activity through reversible oxidation of the active-site cysteine residue is emerging as a general, yet poorly characterized, regulatory mechanism. In this study, we describe a generic antibody-based method for detection of oxidation-inactivated PePs. Previous observations of oxidation of receptor-like PTP (RPTP) [alpha] after treatment of cells with [H.sub.2][O.sub.2] were confirmed. Platelet-derived growth factor (PDGF)-induced oxidation of endogenous SHP-2, sensitive to treatment with the phosphatidylinositol 3-kinase inhibitor LY294002, was demonstrated. Furthermore, oxidation of RPTP[alpha] was shown after UV-irradiation. Interestingly, the catalytically inactive second PTP domain of RPTP[alpha] demonstrated higher susceptibility to oxidation. The experiments thus demonstrate previously unrecognized intrinsic differences between PTP domains to susceptibility to oxidation and suggest mechanisms for regulation of RPTPs with tandem PTP domains. The antibody strategy for detection of reversible oxidation is likely to facilitate further studies on regulation of PTPs and might be applicable to analysis of redox regulation of other enzyme families with active-site cysteine residues.

Published
2004

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