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1. Data from Chk1 Targeting Reactivates PP2A Tumor Suppressor Activity in Cancer Cells

Author

Khanna, Anchit, primary, Kauko, Otto, primary, Böckelman, Camilla, primary, Laine, Anni, primary, Schreck, Ilona, primary, Partanen, Johanna I., primary, Szwajda, Agnieszka, primary, Bormann, Stefanie, primary, Bilgen, Turker, primary, Helenius, Merja, primary, Pokharel, Yuba R., primary, Pimanda, John, primary, Russel, Mike R., primary, Haglund, Caj, primary, Cole, Kristina A., primary, Klefström, Juha, primary, Aittokallio, Tero, primary, Weiss, Carsten, primary, Ristimäki, Ari, primary, Visakorpi, Tapio, primary, and Westermarck, Jukka, primary

Published
2023
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4. Supplementary Figure 5 from Chk1 Targeting Reactivates PP2A Tumor Suppressor Activity in Cancer Cells

Author

Khanna, Anchit, primary, Kauko, Otto, primary, Böckelman, Camilla, primary, Laine, Anni, primary, Schreck, Ilona, primary, Partanen, Johanna I., primary, Szwajda, Agnieszka, primary, Bormann, Stefanie, primary, Bilgen, Turker, primary, Helenius, Merja, primary, Pokharel, Yuba R., primary, Pimanda, John, primary, Russel, Mike R., primary, Haglund, Caj, primary, Cole, Kristina A., primary, Klefström, Juha, primary, Aittokallio, Tero, primary, Weiss, Carsten, primary, Ristimäki, Ari, primary, Visakorpi, Tapio, primary, and Westermarck, Jukka, primary

Published
2023
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5. Supplementary Methods, Figure Legend from Chk1 Targeting Reactivates PP2A Tumor Suppressor Activity in Cancer Cells

Author

Khanna, Anchit, primary, Kauko, Otto, primary, Böckelman, Camilla, primary, Laine, Anni, primary, Schreck, Ilona, primary, Partanen, Johanna I., primary, Szwajda, Agnieszka, primary, Bormann, Stefanie, primary, Bilgen, Turker, primary, Helenius, Merja, primary, Pokharel, Yuba R., primary, Pimanda, John, primary, Russel, Mike R., primary, Haglund, Caj, primary, Cole, Kristina A., primary, Klefström, Juha, primary, Aittokallio, Tero, primary, Weiss, Carsten, primary, Ristimäki, Ari, primary, Visakorpi, Tapio, primary, and Westermarck, Jukka, primary

Published
2023
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6. Supplementary Figure 1 from Chk1 Targeting Reactivates PP2A Tumor Suppressor Activity in Cancer Cells

Author

Khanna, Anchit, primary, Kauko, Otto, primary, Böckelman, Camilla, primary, Laine, Anni, primary, Schreck, Ilona, primary, Partanen, Johanna I., primary, Szwajda, Agnieszka, primary, Bormann, Stefanie, primary, Bilgen, Turker, primary, Helenius, Merja, primary, Pokharel, Yuba R., primary, Pimanda, John, primary, Russel, Mike R., primary, Haglund, Caj, primary, Cole, Kristina A., primary, Klefström, Juha, primary, Aittokallio, Tero, primary, Weiss, Carsten, primary, Ristimäki, Ari, primary, Visakorpi, Tapio, primary, and Westermarck, Jukka, primary

Published
2023
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8. Supplementary Table 2 from Chk1 Targeting Reactivates PP2A Tumor Suppressor Activity in Cancer Cells

Author

Khanna, Anchit, primary, Kauko, Otto, primary, Böckelman, Camilla, primary, Laine, Anni, primary, Schreck, Ilona, primary, Partanen, Johanna I., primary, Szwajda, Agnieszka, primary, Bormann, Stefanie, primary, Bilgen, Turker, primary, Helenius, Merja, primary, Pokharel, Yuba R., primary, Pimanda, John, primary, Russel, Mike R., primary, Haglund, Caj, primary, Cole, Kristina A., primary, Klefström, Juha, primary, Aittokallio, Tero, primary, Weiss, Carsten, primary, Ristimäki, Ari, primary, Visakorpi, Tapio, primary, and Westermarck, Jukka, primary

Published
2023
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9. Supplementary Figure 4 from Chk1 Targeting Reactivates PP2A Tumor Suppressor Activity in Cancer Cells

Author

Khanna, Anchit, primary, Kauko, Otto, primary, Böckelman, Camilla, primary, Laine, Anni, primary, Schreck, Ilona, primary, Partanen, Johanna I., primary, Szwajda, Agnieszka, primary, Bormann, Stefanie, primary, Bilgen, Turker, primary, Helenius, Merja, primary, Pokharel, Yuba R., primary, Pimanda, John, primary, Russel, Mike R., primary, Haglund, Caj, primary, Cole, Kristina A., primary, Klefström, Juha, primary, Aittokallio, Tero, primary, Weiss, Carsten, primary, Ristimäki, Ari, primary, Visakorpi, Tapio, primary, and Westermarck, Jukka, primary

Published
2023
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10. Supplementary Figure 2 from Chk1 Targeting Reactivates PP2A Tumor Suppressor Activity in Cancer Cells

Author

Khanna, Anchit, primary, Kauko, Otto, primary, Böckelman, Camilla, primary, Laine, Anni, primary, Schreck, Ilona, primary, Partanen, Johanna I., primary, Szwajda, Agnieszka, primary, Bormann, Stefanie, primary, Bilgen, Turker, primary, Helenius, Merja, primary, Pokharel, Yuba R., primary, Pimanda, John, primary, Russel, Mike R., primary, Haglund, Caj, primary, Cole, Kristina A., primary, Klefström, Juha, primary, Aittokallio, Tero, primary, Weiss, Carsten, primary, Ristimäki, Ari, primary, Visakorpi, Tapio, primary, and Westermarck, Jukka, primary

Published
2023
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11. Supplementary Figure 3 from Chk1 Targeting Reactivates PP2A Tumor Suppressor Activity in Cancer Cells

Author

Khanna, Anchit, primary, Kauko, Otto, primary, Böckelman, Camilla, primary, Laine, Anni, primary, Schreck, Ilona, primary, Partanen, Johanna I., primary, Szwajda, Agnieszka, primary, Bormann, Stefanie, primary, Bilgen, Turker, primary, Helenius, Merja, primary, Pokharel, Yuba R., primary, Pimanda, John, primary, Russel, Mike R., primary, Haglund, Caj, primary, Cole, Kristina A., primary, Klefström, Juha, primary, Aittokallio, Tero, primary, Weiss, Carsten, primary, Ristimäki, Ari, primary, Visakorpi, Tapio, primary, and Westermarck, Jukka, primary

Published
2023
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12. Supplementary Table 1 from Chk1 Targeting Reactivates PP2A Tumor Suppressor Activity in Cancer Cells

Author

Khanna, Anchit, primary, Kauko, Otto, primary, Böckelman, Camilla, primary, Laine, Anni, primary, Schreck, Ilona, primary, Partanen, Johanna I., primary, Szwajda, Agnieszka, primary, Bormann, Stefanie, primary, Bilgen, Turker, primary, Helenius, Merja, primary, Pokharel, Yuba R., primary, Pimanda, John, primary, Russel, Mike R., primary, Haglund, Caj, primary, Cole, Kristina A., primary, Klefström, Juha, primary, Aittokallio, Tero, primary, Weiss, Carsten, primary, Ristimäki, Ari, primary, Visakorpi, Tapio, primary, and Westermarck, Jukka, primary

Published
2023
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18. Androgen regulation of the androgen receptor coregulators

Author

Helenius Merja A, Suikki Hanna E, Waltering Kati K, Urbanucci Alfonso, and Visakorpi Tapio

Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Abstract Background The critical role of the androgen receptor (AR) in the development of prostate cancer is well recognized. The transcriptional activity of AR is partly regulated by coregulatory proteins. It has been suggested that these coregulators could also be important in the progression of prostate cancer. The aim of this study was to identify coregulators whose expression is regulated by either the androgens and/or by the expression level of AR. Methods We used empty vector and AR cDNA-transfected LNCaP cells (LNCaP-pcDNA3.1, and LNCaP-ARhi, respectively), and grew them for 4 and 24 hours in the presence of dihydrotestosterone (DHT) at various concentrations. The expression of 25 AR coregulators (SRC1, TIF2, PIAS1, PIASx, ARIP4, BRCA1, β-catenin, AIB3, AIB1, CBP, STAT1, NCoR1, AES, cyclin D1, p300, ARA24, LSD1, BAG1L, gelsolin, prohibitin, JMJD2C, JMJD1A, MAK, PAK6 and MAGE11) was then measured by using real-time quantitative RT-PCR (Q-RT-PCR). Results Five of the coregulators (AIB1, CBP, MAK, BRCA1 and β-catenin) showed more than 2-fold induction and 5 others (cyclin D1, gelsolin, prohibitin, JMJD1A, and JMJD2C) less than 2-fold induction. Overexpression of AR did not affect the expression of the coregulators alone. However, overexpression of AR enhanced the DHT-stimulated expression of MAK, BRCA1, AIB1 and CBP and reduced the level of expression of β-catenin, cyclinD1 and gelsolin. Conclusion In conclusion, we identified 5 coactivators whose expression was induced by androgens suggesting that they could potentiate AR signaling. Overexpression of AR seems to sensitize cells for low levels of androgens.

Published
2008
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21. CIP2A is a candidate therapeutic target in clinically challenging prostate cancer cell populations

Author

Khanna, Anchit, Kane, Rane Jayant, Kivinummi, Kati, Urbanucci, Alfonso, Helenius, Merja, Tolonen, Teemu, Saramäki, Outi, Latonen, Leena, Manni, Visa, Pimanda, John, Maitland, Norman, Westermarck, Jukka, Visakorpi, Tapio, BioMediTech - BioMediTech, and University of Tampere

Subjects
Biolääketieteet - Biomedicine, Syöpätaudit - Cancers
Abstract

Residual androgen receptor (AR)-signaling and presence of cancer stem-like cells (SCs) are the two emerging paradigms for clinically challenging castration-resistant prostate cancer (CRPC). Therefore, identification of AR-target proteins that are also overexpressed in the cancer SC population would be an attractive therapeutic approach. Our analysis of over three hundred clinical samples and patient-derived prostate epithelial cultures (PPECs), revealed Cancerous inhibitor of protein phosphatase 2A (CIP2A) as one such target. CIP2A is significantly overexpressed in both hormone-naïve prostate cancer (HN-PC) and CRPC patients . CIP2A is also overexpressed, by 3- and 30-fold, in HN-PC and CRPC SCs respectively. In vivo binding of the AR to the intronic region of CIP2A and its functionality in the AR-moderate and AR-high expressing LNCaP cell-model systems is also demonstrated. Further, we show that AR positively regulates CIP2A expression, both at the mRNA and protein level. Finally, CIP2A depletion reduced cell viability and colony forming efficiency of AR-independent PPECs as well as AR-responsive LNCaP cells, in which anchorage-independent growth is also impaired. These findings identify CIP2A as a common denominator for AR-signaling and cancer SC functionality, highlighting its potential therapeutic significance in the most clinically challenging prostate pathology: castration-resistant prostate cancer. This article has supplementary files, which can be found here:http://dx.doi.org/10.18632/oncotarget.3875

Published
2015

22. CIP2A is a candidate therapeutic target in clinically challenging prostate cancer cell populations

Author

Khanna, Anchit, primary, Rane, Jayant K., additional, Kivinummi, Kati K., additional, Urbanucci, Alfonso, additional, Helenius, Merja A., additional, Tolonen, Teemu T., additional, Saramäki, Outi R., additional, Latonen, Leena, additional, Manni, Visa, additional, Pimanda, John E., additional, Maitland, Norman J., additional, Westermarck, Jukka, additional, and Visakorpi, Tapio, additional

Published
2015
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24. Chk1 Targeting Reactivates PP2A Tumor Suppressor Activity in Cancer Cells

Author

Khanna, Anchit, primary, Kauko, Otto, additional, Böckelman, Camilla, additional, Laine, Anni, additional, Schreck, Ilona, additional, Partanen, Johanna I., additional, Szwajda, Agnieszka, additional, Bormann, Stefanie, additional, Bilgen, Turker, additional, Helenius, Merja, additional, Pokharel, Yuba R., additional, Pimanda, John, additional, Russel, Mike R., additional, Haglund, Caj, additional, Cole, Kristina A., additional, Klefström, Juha, additional, Aittokallio, Tero, additional, Weiss, Carsten, additional, Ristimäki, Ari, additional, Visakorpi, Tapio, additional, and Westermarck, Jukka, additional

Published
2013
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25. Detection of smell print differences between nonmalignant and malignant prostate cells with an electronic nose

Author

Roine, Antti, primary, Tolvanen, Mikko, additional, Sipiläinen, Miki, additional, Kumpulainen, Pekka, additional, Helenius, Merja A, additional, Lehtimäki, Terho, additional, Vepsäläinen, Jouko, additional, Keinänen, Tuomo A, additional, Häkkinen, Merja R, additional, Koskimäki, Juha, additional, Veskimäe, Erik, additional, Tuokko, Antti, additional, Visakorpi, Tapio, additional, Tammela, Teuvo L, additional, Sioris, Thanos, additional, Paavonen, Timo, additional, Lekkala, Jukka, additional, Helle, Hannu, additional, and Oksala, Niku KJ, additional

Published
2012
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35. Molecular cloning nucleotide sequence of chicken avidin-related genes 1-5.

Author

Keinanen, Riitta A., Wallén, Mika J., Kristo, Paula A., Laukkanen, Mikko 0., Toimela, Tarja A., Helenius, Merja A., and Kulomaa, Markku S.

Subjects
AVIDIN, MOLECULAR cloning, NUCLEOTIDE sequence, ALBUMINS
Abstract

Using avidin eDNA as a hybridisation probe, we detected a gene family whose putative products are related lo the chicken egg-white avidin. Two overlapping genomic clones were found to contain five genes favidin-relaled genes I-5. avrl-avr5), which have been cloned, characterized and sequenced. All of the genes have a four-exon structure with an overall identity with the avidin eDNA of 88-92%. The genes appear to have no pseudogenic features and. in fact. two of these genes have been shown to be transcribed. The putative proteins share a sequence identity of 68-78ck with avidin. The amino acid residues responsible for the biotin-binding activity of avidin and the bacterial biotin-binding protein, streptavidin, are highly conserved. Since avidin is induced in both a progesterone-specific manner and in connection with inflammation, these genes offer a valuable tool to study complex gene regulation m vivo. [ABSTRACT FROM AUTHOR]

Published
1994
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36. Aging-induced Up-regulation of Nuclear Binding Activities of Oxidative Stress Responsive NF-kB Transcription Factor in Mouse Cardiac Muscle

Author

Helenius, Merja, Ha¨nninen, Maarit, Lehtinen, Sanna K., and Salminen, Antero

Abstract

The accumulation of lipofuscin to cardiomyocytes is a classical parameter of aging and is believed to reflect oxidative stress. NF-kB transcription factor complex is one of the cellular sensors which responds to oxidative stress and regulates gene expression. Our purpose was to study whether aging affects the level and distribution of DNA binding activities of NF-kB transcription factors both in cardiac sarcoplasm and nuclear extracts. We used electrophoretic mobility shift assays (EMSA) to characterize the DNA binding activities of NF-kB and two other transcription factors, AP-1 and Sp-1, in the myocardium of 4 months and 24 months old male and female NMRI-mice. The protein levels of p50, p52, and p65 components of NF-kB-complex and an inhibitory IkB-α/MAD-3 were assayed with Western blots. Surprisingly, aging up-regulated by 123% the nuclear NF-kB binding activity in the male and female mice. The sarcoplasmic NF-kB activity, activated by deoxycholate, did not show any change during aging. Aging-induced increase in nuclear NF-kB protein-DNA binding activity was observed both by gel retardation and UV-crosslinking assays. In immunoblotting, the level of p52 component but not those of p50 and p65 components of NF-kB-complex was slightly increased in nuclear fractions. Aging did not affect the sarcoplasmic levels of p50, p52, and p65 proteins. Supershift EMSA assays showed that the nuclear NF-kB complex contained p50, p52, and p65 components. The level of inhibitory IkB-α/MAD-3 protein was unaffected by aging both in nuclear and sarcoplasmic fractions. Aging down-regulated the nuclear Sp-1 binding activities but did not affect AP-1 binding activities. Statistically significant sex-related differences did not appear in the aging responses of transcription factors. These results indicate that NF-kB transcription factor pathway is activated during aging in cardiac muscle and could be the signaling route regulating gene expression. However, the activation mechanism of NF-kB during aging whether oxidative stress responsive or notin vivoneeds further studies.

Published
1996
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