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During a hospital-wide prospective point prevalence survey of faecal carriage and environmental colonisation of vancomycin-resistant enterococci in a tertiary care university hospital in Athens (Greece), five clinical and one environmental isolate from a light switch (all in the haematology ward) were identified as vancomycin- and linezolid-resistant vanA-positive Enterococcus faecium (VLRE). The studied isolates exhibited a linezolid minimum inhibitory concentration of 12microg/mL and carried at least one mutated copy of the 23S rRNA gene, as shown by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis to detect the G2576T mutation. The enterococcal surface protein (esp) gene was detected by PCR in all isolates. Molecular typing with pulsed field gel electrophoresis (PFGE) showed that the environmental and four of the five clinical isolates were genetically related. None of the colonised patients were previously exposed to linezolid, although heavy linezolid use was noted in the institution. A case-control study was performed to assess risk factors for VLRE colonisation. In univariate analysis, immunodeficiency, underlying haematological malignancy, duration of any antimicrobial treatment before VLRE isolation, and hospitalisation in the haematology ward were pointed out as possible risk factors. A multidisciplinary approach including intensified hand hygiene, patient contact isolation, disinfection of the inanimate environment and antibiotic restriction resulted in early containment of the VLRE colonisation outbreak.

Hand hygiene is the cornerstone of prevention of nosocomial infections and a challenge for infection-control teams.Bed-rail dispensers of alcohol-based hand rub antiseptic (AHRA) were first initiated only in department A (period I), followed by department B (period II). Opportunities for hand hygiene (OHH) were recorded during periods I, II, and III (post-trial follow-up). Only application of AHRA before and after contacting each patient was considered as appropriate. Comparative evaluation between periods I and II, as well as II and III, was performed. Predictors of AHRA compliance were also assessed by regression analysis.HCWs' compliance was improved after the initiation of the bed-rail approach in department B (51.5%, vs 36.4% P = .005). In department A, where this system already existed, no changes were observed. The bed-rail AHRA system (P = .007 [OR 1.8(1.2-3.0)]) and nurses (P.0001[OR 5.6 (3.1-9.9)]) were predictors of hand hygiene in department B. HCWs' compliance declined in department B (26.5% vs 51.5%, P.0001) and department A (27.5% vs 35.9%, P = .1) during period III.The bed-rail approach initially improved HCWs' compliance with AHRA but did not radically influence behavior in internal medicine settings. Multidisciplinary strategies are required to establish hand hygiene recommendations.