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Polymers, macromolecules in nature and bio-polymers like proteins and antibodies are present in our every-day life and play an important role in our economy. They are obviously present as plastic products (e.g., containers, clothing, car tires) or components, e.g., as expedients in pharmaceutical formulations, additives in food and feed, stabilizers in cosmetics, used in construction materials, ceramics and are key in microchip production. Size-exclusion chromatography is the most important characterization technique for macromolecules. This review covers applications, instrumental setup, step-by-step guides for performing experiments and covers theoretical background as well as troubleshooting and tips and tricks.

Validation is a time consuming process, consisting of many steps. OQ/PV for GPC/SEC systems can be efficiently done with PSS EasyValid, a tool that ensures fast and accurate validation and provides typical GPC/SEC results. It is suitable for all GPC/SEC systems, independent on instrument model, detector type and system manufacturer. [ABSTRACT FROM AUTHOR]

Describes the use of size exclusion chromatography for the characterization of molecules differing in size and molar mass. Determination of molar mass distributions and molar mass averages; Comparison of the chromatograms of a stock solution; Measurement of filtrate on a conventional gas chromatography system/gel permeation chromatography.