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8 results on '"Heizmann, Ulrike"'

1. Cytokine Hemoadsorption During Cardiac Surgery Versus Standard Surgical Care for Infective Endocarditis (REMOVE): Results From a Multicenter Randomized Controlled Trial

Author

Diab, Mahmoud, primary, Lehmann, Thomas, additional, Bothe, Wolfgang, additional, Akhyari, Payam, additional, Platzer, Stephanie, additional, Wendt, Daniel, additional, Deppe, Antje-Christin, additional, Strauch, Justus, additional, Hagel, Stefan, additional, Günther, Albrecht, additional, Faerber, Gloria, additional, Sponholz, Christoph, additional, Franz, Marcus, additional, Scherag, André, additional, Velichkov, Ilia, additional, Silaschi, Miriam, additional, Fassl, Jens, additional, Hofmann, Britt, additional, Lehmann, Sven, additional, Schramm, Rene, additional, Fritz, Georg, additional, Szabo, Gabor, additional, Wahlers, Thorsten, additional, Matschke, Klaus, additional, Lichtenberg, Artur, additional, Pletz, Mathias W., additional, Gummert, Jan F., additional, Beyersdorf, Friedhelm, additional, Hagl, Christian, additional, Borger, Michael A., additional, Bauer, Michael, additional, Brunkhorst, Frank M., additional, Doenst, Torsten, additional, Diab, Mahmoud, additional, Gummert, Jan F, additional, Schiller, Isabella, additional, Winter, Marcus, additional, Eichhorn, Cornelia, additional, Helbig, Aicko, additional, Rißner, Florian, additional, Abdyvasiev, Kubanychbek, additional, Moschovas, Alexandros, additional, Freiburger, Sebastian, additional, Safarov, Rauf, additional, Bargenda, Steffen, additional, Kirov, Hristo, additional, Richter, Markus, additional, Sandhaus, Tim, additional, Zeynalov, Natig, additional, Kaluza, Mirko, additional, Bösemann, Denis, additional, Boog, Swen, additional, Ostermann, Romanus, additional, Christian Schulze, P., additional, Hamadanchi, Ali, additional, Musleh, Rita, additional, Witte, Otto W., additional, Bloos, Petra, additional, Schwope, Katrin, additional, Kolanos, Steffi, additional, Knuhr-Kohlberg, Karina, additional, Haucke, Anja, additional, Lehmann-Pohl, Katja, additional, Weber, Carolyn, additional, Cizmic, Amila, additional, Grathwohl, Corinna, additional, Sindhu, Dirk, additional, Schaschek, Markus, additional, Baier, Axel, additional, Schaub, Sebastian, additional, Großkurth, Uwe, additional, Sone Herré, Eranya, additional, Vlasov, Andrey, additional, Podanev, Dimitry, additional, Plettenberg, Tobias, additional, Mühlbauer, Thomas, additional, Tunggal, Irawati, additional, Eichler, Madlen, additional, Cosic, Jasmina, additional, Wolf, Vera, additional, Krause, Petra, additional, Krischer, Stephanie, additional, Thielmann, Matthias, additional, Wiese, Ingo, additional, Hillmer, Tobias, additional, von Manstein, Jörg, additional, Deus, Markus, additional, Ljesic, Dusko, additional, Roloff, Daniel, additional, Alabowicz Wolfgang Ristau, Tim, additional, Spangel, Gina, additional, Kroll, Johannes, additional, Ballazhi, Fatos, additional, Kondov, Stoyan, additional, Siepe, Matthias, additional, Rylski, Bartosz, additional, Scheumann, Johannes, additional, D’Inka, Matthias, additional, Eschenhagen, Matthias, additional, Al-Hamami, Yasir, additional, Bauer, Nils, additional, Thoma, Martin, additional, Rambach, Albena, additional, Ziemann, Christian, additional, Fernandez Baca Garcia, Gustavo, additional, Morlock, Julia, additional, Benk, Christoph, additional, Klink, Alina, additional, Maltes, Christoph, additional, Lechner, Gabriele, additional, Blümel, Veronika, additional, Heizmann, Ulrike, additional, Kotzjan, Priscilla, additional, Haldenwang, Peter, additional, Elghannam, Mahmoud, additional, Useini, Dritan, additional, Buchwald, Dirk, additional, Buchwald, Bärbel, additional, Schröter, Thomas, additional, Binner, Christian, additional, Hartung, Philipp, additional, Holzhey, David, additional, Misfeld, Martin, additional, Etz, Christian, additional, Davierwala, Piroze, additional, Leontyev, Sergey, additional, Pfannmüller, Bettina, additional, Garbade, Jens, additional, von Aspern, Konstantin, additional, Saeed, Diyar, additional, Aydin, Muhammed, additional, Herzfeld, Jonathan, additional, Feder, Stefan, additional, Kiefer, Philipp, additional, Meyer, Anna, additional, Seeburger, Joerg, additional, Münch, Philipp, additional, Prehl, Jörg, additional, Ginther, René, additional, Koch, Josephine, additional, Hecht, Salome, additional, Winkler, Hannes, additional, Fritzsche, Berit, additional, Winata, Johann, additional, Schmidt, Julia, additional, Labus, Jakob, additional, Frei, Isabell, additional, Schmidt, Volker, additional, Plötze, Katrin, additional, Schal, Susanne, additional, Immohr, Moritz, additional, Sugimura, Yukiharu, additional, Gietmann, Anne, additional, Simm, Andreas, additional, Höpfner, Florian, additional, Stiller, Markus, additional, Ludwig, Kathrin, additional, Helms, Sven, additional, Sunavsky, Jakub, additional, Götte, Julia, additional, Rudloff, Markus, additional, Schönbrodt, Andrea, additional, Fink, Swetlana, additional, Albrecht, Ina-Maria, additional, Minko Nnanga, Alice Huguette, additional, Schneider, Carola, additional, Schilling, Heike, additional, Maier, Tanja, additional, Kühnel, Ralf-Uwe, additional, Ioannou, Stelios, additional, Necaev, Anna-Maria, additional, Müller, Torsten, additional, Bienek, Ralph, additional, Treede, Hendrik, additional, Kohistani, Zaki, additional, Ahmadpour, Touraj, additional, Friese, Sonja, additional, Oberbach, Andreas, additional, Luehr, Maximilian, additional, Joskowiak, Dominik, additional, and Büch, Joscha, additional

Published
2022
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3. Distinct cytokine pattern in aqueous humor during immune reactions following penetrating keratoplasty

Author

Maier, Philip, Heizmann, Ulrike, Böhringer, Daniel, Kern, Yvonne, and Reinhard, Thomas

Subjects
Aqueous Humor, Male, Cytokines, Humans, Female, Middle Aged, Keratoplasty, Penetrating, Research Article, Aged
Abstract

Purpose Cytokine patterns in the aqueous humor before penetrating keratoplasty might permit us to predict the development of immune reactions. We therefore analyzed ten different cytokines in the aqueous humor of patients before and following penetrating keratoplasty. Methods We collected aqueous humor from patients undergoing cataract extraction or penetrating keratoplasty (baseline, n=197), from patients undergoing cataract extraction after penetrating keratoplasty without signs of an endothelial immune reaction (acceptors, n=33), and from patients who underwent irrigation of the anterior chamber due to endothelial immune reactions (rejectors, n=25). We determined interleukin (IL)-1b, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, interferon (INF)-γ, and tumor necrosis factor-α in the aqueous humor, using cytometric bead arrays. Results We found statistically significant differences for IL-10 and INF-γ among the study groups. We observed no significant differences concerning all other cytokines evaluated in this study. Conclusions Elevated levels of INF-γ emphasize its important role in immune reactions following penetrating keratoplasty. Antagonizing INF-γ might be an option when treating immune reactions. High levels of IL-10 during endothelial immune reactions may reflect a downregulation of the delayed-type hypersensitivity. Perhaps this is a donor-specific activation of the immune privilege in the anterior chamber as an attempt to prevent damage during immune reactions. We plan to investigate, in upcoming prospective trials, whether the analysis of cytokine patterns in the aqueous humor before penetrating keratoplasty will enable us to predict the occurrence of immune reactions.

Published
2010

4. Proteomics highlights decrease of matricellular proteins in left ventricular assist device therapy†

Author

Shahinian, Jasmin Hasmik, primary, Mayer, Bettina, additional, Tholen, Stefan, additional, Brehm, Kerstin, additional, Biniossek, Martin L., additional, Füllgraf, Hannah, additional, Kiefer, Selina, additional, Heizmann, Ulrike, additional, Heilmann, Claudia, additional, Rüter, Florian, additional, Grapow, Martin, additional, Reuthebuch, Oliver Thomas, additional, Eckstein, Friedrich, additional, Beyersdorf, Friedhelm, additional, Schilling, Oliver, additional, and Siepe, Matthias, additional

Published
2017
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6. Predicting the risk for corneal graft rejection by aqueous humor analysis.

Author

Maier P, Heizmann U, Böhringer D, Kern Y, and Reinhard T

Subjects
Adult, Age Factors, Aged, Aged, 80 and over, Aqueous Humor chemistry, Cornea pathology, Cornea surgery, Corneal Diseases pathology, Corneal Diseases surgery, Female, Graft Rejection immunology, Graft Rejection metabolism, Humans, Immunity, Cellular, Interferon-gamma analysis, Interferon-gamma immunology, Interleukin-2 analysis, Interleukin-4 analysis, Interleukin-4 immunology, Interleukin-5 analysis, Keratoplasty, Penetrating, Longitudinal Studies, Male, Middle Aged, Predictive Value of Tests, Proportional Hazards Models, Risk Factors, Aqueous Humor immunology, Graft Rejection diagnosis, Interferon-gamma adverse effects, Interleukin-2 immunology, Interleukin-4 adverse effects, Interleukin-5 immunology
Abstract

Purpose: Cytokine patterns determined in the aqueous humor before penetrating keratoplasty (PK) may enable us to predict immune reactions (IR). We therefore analyzed 6 cytokines in the aqueous humor of patients before PK. By prospective clinical follow-up, we tested whether patients who developed an IR would present different preoperative cytokine patterns compared to patients without IR., Methods: We analyzed 18 samples of aqueous humor from 18 patients undergoing PK. The following cytokines were analyzed by cytometric bead array: interleukin 2 (IL-2), interleukin 4 (IL-4), interleukin 5 (IL-5), interleukin 10 (IL-10), tumor-necrosis-factor α (TNF-α), and interferon γ (INF-γ). Seven patients presented with signs of IR during follow up. We performed Cox proportional hazards analysis to determine significant predictors for IR. We iteratively eliminated all co-variates with p values over 0.1 from the survival model (backward selection)., Results: Our final Cox model included the hazardous factors IL-4 (p=0.043) and INF-γ (p=0.059), protective factors IL-2 (p=0.081), IL-5 (p=0.028), and age at time of surgery (p=0.029). We performed a linear discriminant analysis based on these coefficients. The resulting function was: (-9.979*IL5) + (9.262*IL4) + (-3.928*IL2) + (1.709*IFN-γ) + (-0.183*age). A median of -4.97 separated patients with and without IR with no classification error., Conclusions: We demonstrate that cytokine levels in the aqueous humor can be predictive for IR. Our method allowed an almost 100% separation between patients with and without IR. This finding has the potential to improve the aftercare of PK fundamentally. However, our results need to be confirmed in a larger prospective cohort.

Published
2011

7. Active transforming growth factor-beta2 is increased in the aqueous humor of keratoconus patients.

Author

Maier P, Broszinski A, Heizmann U, Böhringer D, and Reinhardau T

Subjects
Adult, Aged, Female, Humans, Male, Middle Aged, Aqueous Humor metabolism, Keratoconus metabolism, Transforming Growth Factor beta2 metabolism
Abstract

Purpose: The secretion of transforming growth factor-beta2 (TGF-beta2) into the aqueous humor was identified to be important for the maintenance of the immunological privilege in the anterior chamber of the eye. Therefore, prognosis of penetrating corneal grafts might depend on intraocular levels of TGF-beta2. In this study, we determined active TGF-beta2 levels in eyes with pathological corneas prior to penetrating keratoplasty (PK)., Methods: Anterior chamber puncture was performed in 38 cataract patients without PK (group I), in 15 keratoconus eyes undergoing PK (group II), and in 66 eyes with various indications for PK (group III). About 0.05-0.1 ml of aqueous humor were harvested from each patient. Analysis of active TGF-beta2 was started via ELISA within three h following puncture., Results: Average active TGF-beta2 concentration was 39.9+/-24.3 pg/ml (median 41.1 pg/ml) in group I, 78.9+/-53.8 pg/ml (median 85.8 pg/ml) in group II, and 31.5+/-37.7 pg/ml (median 23.6 pg/ml) in group III. The differences between group II and groups I and III were statistically significant (p<0.001)., Conclusions: The highest levels of active TGF-beta2 were found in keratoconus eyes. This finding might partly explain the excellent prognosis of keratoconus eyes regarding PK. Further (prospective) studies are being carried out to discover whether active TGF-beta2 might serve as predictive parameter for immune reactions following PK.

Published
2007

8. Determination of active TGF-beta2 in aqueous humor prior to and following cryopreservation.

Author

Maier P, Broszinski A, Heizmann U, Boehringer D, and Reinhard T

Subjects
Aged, Cataract complications, Cataract metabolism, Cataract Extraction, Corneal Diseases metabolism, Enzyme-Linked Immunosorbent Assay, Female, Glaucoma complications, Glaucoma metabolism, Glaucoma surgery, Humans, Lasers, Excimer, Male, Middle Aged, Osmolar Concentration, Photorefractive Keratectomy, Aqueous Humor metabolism, Cryopreservation, Eye Diseases metabolism, Transforming Growth Factor beta2 metabolism
Abstract

Transforming growth factor-beta2 (TGF-beta2) is one of the most important immunosuppressive cytokines in the anterior chamber of the eye. It is secreted as a complex with latency-associated peptide as an inactive precursor. Only the activated form of TGF-beta2 can bind to its receptor and induce signaling. To date, the concentration of active TGF-beta2 in aqueous humor was exclusively determined using samples that had been preserved at -80 degrees C. Quantitative measurements of the activated form directly after sampling have not yet been taken. The aim of this study was to investigate the effect of cryopreservation on the concentration of active TGF-beta2 in the aqueous humor. Samples of aqueous humor were drawn from patients with either cataract or a corneal disorder for determination of TGF-beta2 using a Sandwich-ELISA. In group I (n=30, patients with corneal disorders or cataract), one part of each sample was tested for active TGF-beta2 directly after sampling, whereas the remaining material was stored at -80 degrees C for later analysis. Group II consisted of patients undergoing a simple cataract extraction (n=38), and active TGF-beta2 levels were determined within 3 h after sampling. In Group III (n=34, patients with corneal disorders or cataract), active TGF-beta2 was determined within 3 h after puncture, as were total TGF-beta2 levels after acidic activation for each sample. The average level of active TGF-beta2 in the aqueous humor of group I analyzed directly after sampling was 35+/-31 (median 37) pg/ml. In contrast, frozen samples from the same patients showed an average concentration of 155+/-103 (median 152) pg/ml. The average level of active TGF-beta2 in aqueous humor of 38 cataract (group II) eyes was 40+/-24 (median 41) pg/ml determined within 3 h after puncture. The average level of total TGF-beta2 in group III was 1,654+/-631 (median 1,542) pg/ml compared to 33+/-39 (median 28) pg/ml of active TGF-beta2 determined directly after sampling, yielding a ratio of 2% of active to total TGF-beta2. Levels of active TGF-beta2 in aqueous humor determined directly after sampling were 4.4 fold lower than those measured in frozen samples. Thus, samples meant for determining active TGF-beta2 levels should not be kept frozen.

Published
2006

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