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Shannon J Heine1, Denise Olive1, Ji-Liang Gao2, Philip M Murphy2, Michael I Bukrinsky1, Stephanie L Constant11Department of Microbiology, Immunology and Tropical Medicine, The George Washington University, Washington, DC, USA; 2Laboratory of Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USABackground: Chemokines contribute to inflammatory responses by inducing leukocyte migration and extravasation. In addition, chemoattractants other than classical chemokines can also be present. Many chemokines have been demonstrated to cooperate, leading to an augmentation in leukocyte recruitment and providing a potential role for the presence of multiple chemoattractants. Extracellular cyclophilins are a group of alternative chemotactic factors, which can be highly elevated during various inflammatory responses and, as we have previously shown, can contribute significantly to neutrophil recruitment in an animal model of acute lung inflammation. In the current studies we investigated whether the most abundant extracellular cyclophilin, CypA, has the capacity to function in partnership with 2 classical chemokines known to be secreted in the same model, macrophage inflammatory protein (MIP)-2/CXCL2 and keratinocyte chemoattractant (KC)/CXCL1.Methods: Neutrophil migration in response to combinations of CypA and MIP-2 or CypA and KC was measured by in vitro chemotaxis assays. Biochemical responses of neutrophils incubated with the combinations of chemoattractants were determined by changes in chemokine receptor internalization and actin polymerization measured by flow cytometry, and changes in intracellular calcium mobilization measured with a calcium sensitive fluorochrome.Results: A combination of CypA and MIP-2, but not KC, augmented neutrophil migration. Based on the level of augmentation, the cooperation between CypA and MIP-2 appeared to be synergistic. Evidence that CypA and MIP-2 cooperate at the biochemical level was demonstrated by increases in receptor internalization, calcium mobilization, and actin polymerization.Conclusion: These findings provide evidence for the capacity of extracellular cyclophilins to interact with classical chemokines, resulting in greater and more efficient leukocyte recruitment.Keywords: chemokine, chemotaxis, inflammation

The authors investigated compensatory self-enhancement in Japanese and Canadian university students. Research has revealed that when North Americans publicly discover a weakness in one self-domain, they typically bolster their self-assessments in another unrelated domain. This effect is less commonly found in private settings. Following a private failure experience on a creativity task, Canadians discounted the negative feedback, although they did not exhibit a compensatory self-enhancing response. In contrast, Japanese were highly responsive to the failure feedback and showed evidence of reverse compensatory self-enhancement. This study provides further evidence that self-evaluation maintenance strategies are elusive among Japanese samples. [ABSTRACT FROM AUTHOR]

Economic inequality has been found to be associated with increased unethical behavior and an increased acceptance of unethical behavior. In this paper we explored whether higher amounts of perceived inequality lead to an increase in the expectation of unethical behavior. We tested whether people would say that they themselves would engage in more unethical behavior in a context of high compared to low inequality. We find evidence for this hypothesis in 3 of 4 studies (n = 3,038). An internal meta-analysis shows a small but significant effect. Such increased expectations that oneself will behave unethically likely has consequences for societal trust and functioning., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Schmalor et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Diarrhea caused by Shigella has been associated with high morbidity and mortality in young children worldwide. There are no licensed vaccines, and those clinically advanced have restricted coverage as they elicit serotype-specific immunity while disease is caused by multiple circulating serotypes. Our group had previously reported a close association between serum antibodies to the Shigella virulence factor VirG (or IcsA) and clinical protection in infected individuals. VirG is highly conserved among Shigella strains and appealing as a broad-spectrum vaccine candidate. In this study, we investigated the immunogenicity and protective capacity of VirG as a subunit vaccine in mice. The surface-exposed alpha (α) domain of VirG (VirGα) was produced as a recombinant protein. This region has almost identical immune reactivity to full-length VirG. Administered intramuscularly with alum, VirGα elicited robust immune responses and high protective efficacy against S. flexneri 2a and S. sonnei. Almost complete protection was afforded by VirGα given intranasally with the E. coli double mutant heat-labile toxin (dmLT). VirGα-specific antibodies recognized VirG expressed on live Shigella, and blocked Shigella adhesion and invasion to human colonic cells. These results show for the first time that VirGα is a promising cross-protective vaccine candidate to prevent Shigella infection., (© 2024. The Author(s).)

Objective: Drawing from dual-strategies theory, leader-member exchange theory, and several theories of self-esteem, we develop and test hypotheses about how followers' self-esteem predicts their perceptions of dominant and prestigious leaders' leadership ability., Method: Across four studies (N = 1568), we tested the association between self-esteem and perceptions of leadership ability for dominant and prestigious leaders., Results: Individuals with high self-esteem perceived greater leadership ability in prestigious leaders than did those with low self-esteem and individuals with low self-esteem perceived greater leadership ability in dominant leaders than did those with high self-esteem. These results emerged across ratings of leaders from hypothetical vignettes (Studies 1 and 4), abstract beliefs about what constitutes good leadership (Study 1), past personal experiences with leaders (Study 2) and clips of leaders from reality television (Study 3). In Study 4, we also tested potential mechanisms. Compared with followers with low self-esteem, followers with high self-esteem found prestigious leaders more trustworthy, and they anticipated feeling inauthentic around a dominant leader., Conclusions: Self-esteem is reliably and robustly related to perceived leadership ability of dominant and prestigious leaders, and these differences might stem from differences in trust in prestigious leaders and anticipated authenticity around dominant leaders., (© 2022 Wiley Periodicals LLC.)

In the present research we tested the differential effects of anger versus shame as emotional predictors of ingroup disidentification in one rather collectivistic (Japan) and two rather individualistic societies (Germany, Canada). We tested the idea that individuals cope with socially undesired emotions by disidentifying from their group. Specifically, we predicted that after a group conflict, anger, an undesired emotion in Japan, would elicit disidentification in Japan, whereas shame, an undesired emotion in Canada and Germany, would elicit disidentification in Germany and Canada. Study 1 (N = 378) found that anger, but not shame, was related to disidentification in Japan, whereas shame, but not anger, was related to disidentification in Canada and Germany. Study 2 (N = 171) shows that, after group conflict, Japanese disidentified more when imagining to feel angry, whereas Germans disidentified more when imagining to feel ashamed. Implications for these findings are discussed., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Bierle et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

The uncanny valley is a topic for engineers, animators, and psychologists, yet uncanny emotions are without a clear definition. Across three studies, we developed an 8-item measure of unnerved feelings, finding that it was discriminable from other affective experiences. In Study 1, we conducted an exploratory factor analysis that yielded two factors; an unnerved factor, which connects to emotional reactions to the uncanny, and a disoriented factor, which connects to mental state changes more distally following uncanny experiences. Focusing on the unnerved measure, Study 2 tests the scale's convergent and discriminant validity, concluding that participants who watched an uncanny video were more unnerved than those who watched a disgusting, fearful, or a neutral video. In Study 3, we determined that our scale detects unnerved feelings created during early 2020 by the coronavirus pandemic; a distinct source of uncanniness. These studies contribute to the psychological and interdisciplinary understanding of this strange, eerie phenomenon of being confronted with what looms just beyond our understanding.

Recent years have not only seen growing public distrust in science, but also in the people conducting science. Yet, attitudes toward scientists remain largely unexplored, and the limited body of literature that exists points to an interesting ambivalence. While survey data suggest scientists to be positively evaluated (e.g., respected and trusted), research has found scientists to be perceived as capable of immoral behavior. We report two experiments aimed at identifying what contributes to this ambivalence through systematic investigations of stereotypical perceptions of scientists. In these studies, we particularly focus on two potential sources of inconsistencies in previous work: divergent operationalizations of morality (measurement effects), and different specifications of the broad group of scientists (framing effects). Results show that scientists are generally perceived as more likely to violate binding as opposed to individualizing moral foundations, and that they deviate from control groups more strongly on the latter. The extent to which different morality measures reflect the differentiation between binding and individualizing moral foundations at least partially accounts for previous contradictory findings. Moreover, the results indicate large variation in perceptions of different types of scientists: people hold more positive attitudes toward university-affiliated scientists as compared to industry-affiliated scientists, with perceptions of the 'typical scientist' more closely resembling the latter. Taken together, the findings have important academic ramifications for science skepticism, morality, and stereotyping research as well as valuable practical implications for successful science communication., Competing Interests: The authors have declared that no competing interests exist.

Uchiyama et al. question heritability estimates in a convincing manner. We offer additional arguments to further bolster their claims, highlighting methodological issues in heritability coefficients' derivation, their misuse in various contexts, and their potential contributions to exacerbating common erroneous intuitions that have been shown to lead to deleterious social phenomena. We conclude that science should move away from using them.

Across five studies (three preregistered; N = 2,481), we investigated two effects as follows: (1) Is higher subjective economic inequality associated with a decreased ability to accurately identify emotions (emotional intelligence)? When inequality is high, people are less focused on others and may thus be less motivated to correctly identify their emotions. (2) Is this main effect of subjective inequality qualified by an interaction with socioeconomic status (SES)? Past research suggests that high SES leads to lower emotional intelligence because people of higher SES are less dependent on others and thus less motivated to identify their emotions. When perceiving higher inequality, high SES individuals should feel even more self-reliant, thereby exacerbating the difference in emotional intelligence between people of low and high SES. We provide empirical support in three out of five studies for the first and in four out of five studies for the second hypothesis. An internal meta-analysis supported both hypotheses., Competing Interests: Declaration of Conflicting Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (© The Author(s) 2021.)

Economic inequality has been associated with a host of social ills, but most research has focused on objective measures of inequality. We argue that economic inequality also has a subjective component, and understanding the effects of economic inequality will be deepened by considering the ways that people perceive inequality. In an American sample ( N = 1,014), we find that some of the key variables that past research has found to correlate with objective inequality also correlate with a subjective measure of inequality. Across six countries ( N = 683), we find that the relationship between subjective inequality and different psychological variables varies by country. Subjective inequality shows only modest correlations with objective inequality and varies by sociodemographic background., Competing Interests: Declaration of Conflicting Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (© The Author(s) 2021.)

People are regularly exposed to discussions about the role of genes in their lives, despite often having limited understanding about how they operate. The tendency to oversimplify genetic causes, and ascribe them with undue influence is termed genetic essentialism. Two studies revealed that genetic essentialism is associated with support for eugenic policies and social attitudes based in social inequality, and less acceptance of genetically modified foods. These views about eugenics and genetically-modified foods were especially evident among people who had less knowledge about genes, potentially highlighting the value of education in genetics., Competing Interests: The authors have declared that no competing interests exist.

Sleep is a fundamental biological process that all humans exhibit, and there is much evidence that people suffer adverse health outcomes from insufficient sleep. Despite this evidence, much research demonstrates significant heterogeneity in the amounts that people sleep across cultures. This suggests that despite serving fundamental biological functions, sleep is also subject to cultural influence. Using self-report and actigraphy data we examined sleep among European Canadian, Asian Canadian, and Japanese university students. Significant cultural differences emerged in terms of various parameters of sleep (e.g. sleep time), and beliefs about sleep (e.g. perceived relation between sleep and health). Despite sleeping significantly less than European Canadians, Japanese participants slept less efficiently, yet reported being less tired and having better health. Moreover, relative to European Canadians, Japanese participants perceived a weaker relation between sleep and physical health, and had a significantly shorter ideal amount of sleep. Asian Canadians' sleep behaviors and attitudes were largely similar to European Canadians suggesting that people acculturate to local cultural sleep norms., Competing Interests: No authors have competing interests.

Much research has shown that people tend to view genes in rather deterministic ways-often termed genetic essentialism. We explored how people would view the causes of ethnic stereotypes in contexts where human genetic variability was either emphasized or downplayed. In two studies with over 1600 participants we found that people viewed ethnic stereotypes to be more of a function of underlying genetics after they read an article describing how ancestry can be estimated by geographic distributions of gene frequencies than after reading an article describing how relatively homogeneous the human genome was or after reading a control essay. Moreover, people were more likely to attribute ethnic stereotypes to genes when they scored higher on a measure of genetic essentialism or when they had less knowledge about genes. Our understanding of stereotypes is a function of our understanding of genetics., Competing Interests: No competing interests exist.

Our study was designed to examine whether the pain reliever acetaminophen impacts the normal ebb-and-flow of off-task attentional states, such as captured by the phenomenon of mind wandering. In a placebo-controlled between-groups design, participants performed a sustained attention to response task while event-related potentials (ERPs) to target events were recorded. Participants were queried at random intervals for their attentional reports - either "on-task" or "off-task." The frequency of these reports and the ERPs generated by the preceding target events were assessed. Behaviorally, the frequency of off-task attentional reports was comparable between groups. Electrophysiologically, two findings emerged: first, the amplitude of the P300 ERP component elicited by target events was significantly attenuated during off-task vs. on-task attentional states in both the acetaminophen and placebo groups. Second, the amplitude of the LPP ERP component elicited by target events showed a significant decrease during off-task attentional states that was specific to the acetaminophen group. Taken together, our findings support the conclusion that acetaminophen doesn't impact our relative propensity to drift into off-task attentional states, but it does affect the depth of neurocognitive disengagement during off-task attentional states, and in particular, at the level of post-categorization stimulus evaluations indexed by the LPP.

"Psychological essentialism" refers to our tendency to view the natural world as emerging from the result of deep, hidden, and internal forces called "essences." People tend to believe that genes underlie a person's identity. People encounter information about genetics on a regular basis, as through media such as a New York Times piece "Infidelity Lurks in Your Genes" or a 23andMe commercial showing people acquiring new ethnic identities as the result of their genotyping. How do people make sense of new scientific findings that are inherently complex if they don't have years of specialized training and education at their disposal? Given the substantial overlap between a lay understanding of genetics and lay intuitions about essences, we argue that, when most people are thinking about genes, they are not really thinking about genes in the complex ways that good scientists are. Combating people's essentialist biases can be a formidable challenge. Although we have identified some promising results of trying to reduce people's genetic essentialist tendencies, there is still much to learn about how these essentialist biases can be countered. It is important to help people understand genetic information so they are able make well-informed decisions about their lives., (© 2019 The Hastings Center.)

Yersinia enterocolitica causes a severe enteric infection in infants and young children. There is no vaccine approved for use in humans. We investigated the immunogenicity and protective capacity of Yersinia YopB, a conserved type III secretion system protein, alone or combined with LcrV in adult mice immunized intranasally. YopB or LcrV (5 μg) administered with the Escherichia coli double mutant heat-labile toxin (dmLT) adjuvant afforded modest (10-30%) protection against lethal Y. enterocolitica oral infection. The combination of YopB and LcrV (5 μg each) dramatically improved vaccine efficacy (70-80%). Additionally, it afforded complete protection against Y. pestis pulmonary infection. Immunization with YopB/LcrV+dmLT resulted in Ag-specific serum IgG, systemic and mucosal Ab-secreting cells, as well as IFN-γ, TNF-α, IL-2, IL-6, IL-17A, and KC production by spleen cells. Serum Abs elicited by YopB/LcrV+dmLT had enhanced bactericidal and opsonophagocytic killing activity. After Y. enterocolitica challenge, YopB/LcrV+dmLT-vaccinated mice exhibited intact intestinal tissue, active germinal centers in mesenteric lymph nodes, IgG + and IgA + plasmablasts in the lamina propria, and Abs in intestinal fluid. On the contrary, complete tissue destruction and abscesses were seen in placebo recipients that succumbed to infection. Mice immunized as infants with YopB+dmLT or LcrV+dmLT achieved 60% protection against lethal Y. enterocolitica infection, and vaccine efficacy increased to 90-100% when they received YopB/LcrV+dmLT. YopB+dmLT also afforded substantial (60%) protection when administered intradermally to infant mice. YopB/LcrV+dmLT is a promising subunit vaccine candidate with the potential to elicit broad protection against Yersinia spp., (Copyright © 2019 by The American Association of Immunologists, Inc.)

In this investigation of cultural differences in the experience of obligation, we distinguish between Confucian Role Ethics versus Relative Autonomy lay theories of motivation and illustrate them with data showing relevant cultural differences in both social judgments and intrapersonal experience. First, when judging others, Western European heritage culture (WEHC) participants (relative to Confucian heritage culture [CHC] participants) judged obligation-motivated actors more negatively than those motivated by agency (Study 1, N = 529). Second, in daily diary and situation sampling studies, CHC participants (relative to WEHC participants) perceived more congruency between their own agentic and obligated motivations, and more positive emotional associations with obligated motivations (Study 2, N = 200 and Study 3, N = 244). Agentic motivation, however, was universally associated with positive emotions. More research on a Role Ethics rather than Relative Autonomy conception of agency may improve our understanding of human motivation, especially across cultures.

The Meaning Maintenance Model posits that individuals seek to resolve uncertainty by searching for patterns in the environment, yet little is known about how this is accomplished. Four studies investigated whether uncertainty has an effect on people's cognitive functioning. In particular, we investigated whether meaning threats lead to increased working memory capacity. In each study, we exposed participants to either an uncertain stimulus used to threaten meaning in past studies, or a control stimulus. Participants then completed a working memory measure, where they either had to recall lists of words (Studies 1, 2), or strings of digits (Studies 3, 4). We used both a frequentist approach and Bayesian analysis to evaluate our findings. Across the four studies, we find a small but consistent effect, where participants in the meaning threat condition show improved performance on the working memory tasks. Overall, our findings were consistent with the hypothesis that working memory capacity increases when people experience a meaning threat, which may help to explain improved pattern recognition. Additionally, our results highlight the value of using a Bayesian analytic approach, particularly when studying phenomena with high variance., Competing Interests: The authors have declared that no competing interests exist.

Overconfidence is sometimes assumed to be a human universal, but there remains a dearth of data systematically measuring overconfidence across populations and contexts. Moreover, cross-cultural experiments often fail to distinguish between placement and precision and worse still, often compare population-mean placement estimates rather than individual performance subtracted from placement. Here we introduce a procedure for concurrently capturing both placement and precision at an individual level based on individual performance: The Elicitation of Genuine Overconfidence (EGO) procedure. We conducted experiments using the EGO procedure, manipulating domain, task knowledge, and incentives across four populations-Japanese, Hong Kong Chinese, Euro Canadians, and East Asian Canadians. We find that previous measures of population-level overconfidence may have been misleading; rather than universal, overconfidence is highly context dependent. Our results reveal cross-cultural differences in sensitivity to incentives and differences in overconfidence strategies, with underconfidence, accuracy, and overconfidence. Comparing sexes, we find inconsistent results for overplacement, but that males are consistently more confident in their placement. These findings have implications for our understanding of the adaptive value of overconfidence and its role in explaining population-level and individual-level differences in economic and psychological behavior., Competing Interests: The authors have declared that no competing interests exist.

Shigella is an important cause of diarrheal disease in young children living in developing countries. No approved vaccines are available, and the development of vaccine candidates has been hindered by the lack of firm immunological correlates of protection, among other reasons. To address this gap in knowledge, we established quantitative assays to measure Shigella-specific serum bactericidal antibody (SBA) and opsonophagocytic killing antibody (OPKA) activities and investigated their potential association with protection against disease in humans. SBA, OPKA, and Ipa-, VirG (IscA)-, and Shigella flexneri 2a lipopolysaccharide-specific serum IgG titers were determined in adult volunteers who received Shigella vaccine candidate EcSf2a-2 and in unvaccinated controls, all of whom were challenged with virulent Shigella flexneri 2a. Prechallenge antibody titers were compared with disease severity after challenge. SBA and OPKA, as well as IpaB- and VirG-specific IgG, significantly correlated with reduced illness. SBA and OPKA assays were also used to evaluate the immunogenicity of leading live attenuated vaccine candidates Shigella CVD 1204 and CVD 1208S in humans. A single oral immunization with CVD 1204 or CVD 1208S resulted in SBA seroconversion rates of 71% and 47% and OPKA seroconversion rates of 57% and 35%, respectively. Higher functional antibody responses were induced by CVD 1204, which is consistent with its lower attenuation. This is the first demonstration of SBA, OPKA, and IpaB- and VirG-specific IgG levels as potential serological correlates of protection against shigellosis in humans. These results warrant further studies to establish their capacity to predict protective immunity and vaccine efficacy., (Copyright © 2017 Shimanovich et al.)

Acetaminophen has recently been recognized as having impacts that extend into the affective domain. In particular, double blind placebo controlled trials have revealed that acetaminophen reduces the magnitude of reactivity to social rejection, frustration, dissonance and to both negatively and positively valenced attitude objects. Given this diversity of consequences, it has been proposed that the psychological effects of acetaminophen may reflect a widespread blunting of evaluative processing. We tested this hypothesis using event-related potentials (ERPs). Sixty-two participants received acetaminophen or a placebo in a double-blind protocol and completed the Go/NoGo task. Participants' ERPs were observed following errors on the Go/NoGo task, in particular the error-related negativity (ERN; measured at FCz) and error-related positivity (Pe; measured at Pz and CPz). Results show that acetaminophen inhibits the Pe, but not the ERN, and the magnitude of an individual's Pe correlates positively with omission errors, partially mediating the effects of acetaminophen on the error rate. These results suggest that recently documented affective blunting caused by acetaminophen may best be described as an inhibition of evaluative processing. They also contribute to the growing work suggesting that the Pe is more strongly associated with conscious awareness of errors relative to the ERN., (© The Author (2016). Published by Oxford University Press. For Permissions, please email: journals.permissions@oup.com.)

Do people think that scientists are bad people? Although surveys find that science is a highly respected profession, a growing discourse has emerged regarding how science is often judged negatively. We report ten studies (N = 2328) that investigated morality judgments of scientists and compared those with judgments of various control groups, including atheists. A persistent intuitive association between scientists and disturbing immoral conduct emerged for violations of the binding moral foundations, particularly when this pertained to violations of purity. However, there was no association in the context of the individualizing moral foundations related to fairness and care. Other evidence found that scientists were perceived as similar to others in their concerns with the individualizing moral foundations of fairness and care, yet as departing for all of the binding foundations of loyalty, authority, and purity. Furthermore, participants stereotyped scientists particularly as robot-like and lacking emotions, as well as valuing knowledge over morality and being potentially dangerous. The observed intuitive immorality associations are partially due to these explicit stereotypes but do not correlate with any perceived atheism. We conclude that scientists are perceived not as inherently immoral, but as capable of immoral conduct.

Much debate exists surrounding the applicability of genetic information in the courtroom, making the psychological processes underlying how people consider this information important to explore. This article addresses how people think about different kinds of causal explanations in legal decision-making contexts. Three studies involving a total of 600 Mechanical Turk and university participants found that genetic, versus environmental, explanations of criminal behavior lead people to view the applicability of various defense claims differently, perceive the perpetrator's mental state differently, and draw different causal attributions. Moreover, mediation and path analyses highlight the double-edged nature of genetic attributions-they simultaneously reduce people's perception of the perpetrator's sense of control while increasing people's tendencies to attribute the cause to internal factors and to expect the perpetrator to reoffend. These countervailing relations, in turn, predict sentencing in opposite directions, although no overall differences in sentencing or ultimate verdicts were found., (© 2015 by the Society for Personality and Social Psychology, Inc.)

Shigella spp. are among the enteric pathogens with the highest attributable incidence of moderate-to-severe diarrhea in children under 5 years of age living in endemic areas. There are no vaccines available to prevent this disease. In this work, we investigated a new Shigella vaccine concept consisting of nonliving, self-adjuvanted, Lactococcus lactis bacterium-like particles (BLP) displaying Shigella invasion plasmid antigen (Ipa) B and IpaD and examined its immunogenicity and protective efficacy in adult and newborn/infant mice immunized via the nasal route. Unique advantages of this approach include the potential for broad protection due to the highly conserved structure of the Ipas and the safety and practicality of a probiotic-based mucosal/adjuvant delivery platform. Immunization of adult mice with BLP-IpaB and BLP-IpaD (BLP-IpaB/D) induced high levels of Ipa-specific serum IgG and stool IgA in a dose-dependent manner. Immune responses and protection were enhanced by BLP delivery. Vaccine-induced serum antibodies exhibited opsonophagocytic and cytotoxic neutralizing activity, and IpaB/D IgG titers correlated with increased survival post-challenge. Ipa-specific antibody secreting cells were detected in nasal tissue and lungs, as well as IgG in bronchoalveolar lavage. Bone marrow cells produced IpaB/D-specific antibodies and contributed to protection after adoptive transfer. The BLP-IpaB/D vaccine conferred 90% and 80% protection against S. flexneri and S. sonnei, respectively. Mice immunized with BLP-IpaB/D as newborns also developed IpaB and IpaD serum antibodies; 90% were protected against S. flexneri and 44% against S. sonnei. The BLP-IpaB/D vaccine is a promising candidate for safe, practical and potentially effective immunization of children against shigellosis.

Cognitive dissonance theory shares much in common with other perspectives that address anomalies, uncertainty, and general expectancy violations. This has led some theorists to argue that these theories represent overlapping psychological processes. If responding to dissonance and uncertainty occurs through a common psychological process, one should expect that the behavioral outcomes of feeling uncertain would also apply to feelings of dissonance, and vice versa. One specific prediction from the meaning maintenance model would be that cognitive dissonance, like other expectancy violations, should lead to the affirmation of unrelated beliefs, or the abstraction of unrelated schemas when the dissonant event cannot be easily accommodated. This article presents 4 studies (N = 1124) demonstrating that the classic induced-compliance dissonance paradigm can lead not only to a change of attitudes (dissonance reduction), but also to (a) an increased reported belief in God (Study 2), (b) a desire to punish norm-violators (Study 1 and 3), (c) a motivation to detect patterns amid noise (Study 3), and (d) polarizing support of public policies among those already biased toward a particular side (Study 4). These results are congruent with theories that propose content-general fluid compensation following the experience of anomaly, a finding not predicted by dissonance theory. The results suggest that dissonance reduction behaviors may share psychological processes described by other theories addressing violations of expectations., ((c) 2015 APA, all rights reserved).)

Implicit self-esteem (ISE), which is often defined as automatic self-evaluations, fuses research on unconscious processes with that on self-esteem. As ISE is viewed as immune to explicit control, it affords the testing of theoretical questions such as whether cultures vary in self-enhancement motivations. We provide a critical review and integration of the work on (a) the operationalization of ISE and (b) possible cultural variation in self-enhancement motivations. Although ISE measures do not often vary across cultures, recent meta-analyses and empirical studies question the validity of the most common way of defining ISE. We revive an alternative conceptualization that defines ISE in terms of how positively people evaluate objects that reflect upon themselves. This conceptualization suggests that ISE research should target alternative phenomena (e.g., minimal group effect, similarity-attraction effect, endowment effect) and it allows for a host of previous cross-cultural findings to bear on the question of cultural variability in ISE., (© 2014 by the Society for Personality and Social Psychology, Inc.)

Objective: Our research utilized two popular theoretical conceptualizations of implicit self-esteem: 1) implicit self-esteem as a global automatic reaction to the self; and 2) implicit self-esteem as a context/domain specific construct. Under this framework, we present an extensive search for implicit self-esteem measure validity among different cultural groups (Study 1) and under several experimental manipulations (Study 2)., Method: In Study 1, Euro-Canadians (N = 107), Asian-Canadians (N = 187), and Japanese (N = 112) completed a battery of implicit self-esteem, explicit self-esteem, and criterion measures. Included implicit self-esteem measures were either popular or provided methodological improvements upon older methods. Criterion measures were sampled from previous research on implicit self-esteem and included self-report and independent ratings. In Study 2, Americans (N = 582) completed a shorter battery of these same types of measures under either a control condition, an explicit prime meant to activate the self-concept in a particular context, or prime meant to activate self-competence related implicit attitudes., Results: Across both studies, explicit self-esteem measures far outperformed implicit self-esteem measures in all cultural groups and under all experimental manipulations., Conclusion: Implicit self-esteem measures are not valid for individual or cross-cultural comparisons. We speculate that individuals may not form implicit associations with the self as an attitudinal object., (© 2013 Wiley Periodicals, Inc.)

Public discourse on genetic predispositions for obesity has flourished in recent decades. In three studies, we investigated behaviorally-relevant correlates and consequences of a perceived genetic etiology for obesity. In Study 1, beliefs about etiological explanations for obesity were assessed. Stronger endorsement of genetic etiology was predictive of a belief that obese people have no control over their weight. In Study 2, beliefs about weight and its causes were assessed following a manipulation of the perceived underlying cause. Compared with a genetic attribution, a non-genetic physiological attribution led to increased perception of control over one's weight. In Study 3, participants read a fictional media report presenting either a genetic explanation, a psychosocial explanation, or no explanation (control) for obesity. Results indicated that participants who read the genetic explanation ate significantly more on a follow-up task. Taken together, these studies demonstrate potential effects of genetic attributions for obesity., (Copyright © 2014 Elsevier Ltd. All rights reserved.)

Shigella is one of the leading pathogens contributing to the vast pediatric diarrheal disease burden in low-income countries. No licensed vaccine is available, and the existing candidates are only partially effective and serotype specific. Shigella type III secretion system proteins IpaB and IpaD, which are conserved across Shigella spp., are candidates for a broadly protective, subunit-based vaccine. In this study, we investigated the immunogenicity and protective efficacy of IpaB and IpaD administered intradermally (i.d.) with a double-mutant of the Escherichia coli heat-labile enterotoxin (dmLT) adjuvant using microneedles. Different dosage levels of IpaB and IpaD, with or without dmLT, were tested in mice. Vaccine delivery into the dermis, recruitment of neutrophils, macrophages, dendritic cells, and Langerhans cells, and colocalization of vaccine Ag within skin-activated APC were demonstrated through histology and immunofluorescence microscopy. Ag-loaded neutrophils, macrophages, dendritic cells, and Langerhans cells remained in the tissue at least 1 wk. IpaB, IpaD, and dmLT-specific serum IgG- and IgG-secreting cells were produced following i.d. immunization. The protective efficacy was 70% against Shigella flexneri and 50% against Shigella sonnei. Similar results were obtained when the vaccine was administered intranasally, with the i.d. route requiring 25-40 times lower doses. Distinctively, IgG was detected in mucosal secretions; secretory IgA, as well as mucosal and systemic IgA Ab-secreting cells, were seemingly absent. Vaccine-induced T cells produced IFN-γ, IL-2, TNF-α, IL-17, IL-4, IL-5, and IL-10. These results demonstrate the potential of i.d. vaccination with IpaB and IpaD to prevent Shigella infection and support further studies in humans.

A growing body of research has shown that Western vegetarians report more concern for animal welfare and environmental sustainability, and endorse more liberal values than do Western omnivores. However, despite the prevalence of Indian vegetarianism, its psychological associations and underpinnings remain largely unexamined. In Study 1, we find that Euro-American vegetarians are more concerned than omnivores with the impact of their daily food choices on the environment and animal welfare, show more concern for general animal welfare, and endorse universalistic values more, yet among Indian participants, these differences are not significant. In Study 2, we show that Indian vegetarians more strongly endorse the belief that eating meat is polluting, and show a heightened concern for the conservative ethics of Purity, Authority, and Ingroup relative to their omnivorous peers, whereas these differences are largely absent among Euro-Canadians and Euro-Americans., (Copyright © 2013 Elsevier Ltd. All rights reserved.)

Shigella spp. are food- and water-borne pathogens that cause shigellosis, a severe diarrheal and dysenteric disease that is associated with a high morbidity and mortality in resource-poor countries. No licensed vaccine is available to prevent shigellosis. We have recently demonstrated that Shigella invasion plasmid antigens (Ipas), IpaB and IpaD, which are components of the bacterial type III secretion system (TTSS), can prevent infection in a mouse model of intranasal immunization and lethal pulmonary challenge. Because they are conserved across Shigella spp. and highly immunogenic, these proteins are excellent candidates for a cross-protective vaccine. Ideally, such a vaccine could be administered to humans orally to induce mucosal and systemic immunity. In this study, we investigated the immunogenicity and protective efficacy of Shigella IpaB and IpaD administered orally with a double mutant of the Escherichia coli heat labile toxin (dmLT) as a mucosal adjuvant. We characterized the immune responses induced by oral vs. intranasal immunization and the protective efficacy using a mouse pulmonary infection model. Serum IgG and fecal IgA against IpaB were induced after oral immunization. These responses, however, were lower than those obtained after intranasal immunization despite a 100-fold dosage increase. The level of protection induced by oral immunization with IpaB and IpaD was 40%, while intranasal immunization resulted in 90% protective efficacy. IpaB- and IpaD-specific IgA antibody-secreting cells in the lungs and spleen and T-cell-derived IL-2, IL-5, IL-17 and IL-10 were associated with protection. These results demonstrate the immunogenicity of orally administered IpaB and IpaD and support further studies in humans., (Copyright © 2013 Elsevier Ltd. All rights reserved.)

The meaning-maintenance model posits that any violation of expectations leads to an affective experience that motivates compensatory affirmation. We explore whether the neural mechanism that responds to meaning threats can be inhibited by acetaminophen, in the same way that acetaminophen inhibits physical pain or the distress caused by social rejection. In two studies, participants received either acetaminophen or a placebo and were provided with either an unsettling experience or a control experience. In Study 1, participants wrote about either their death or a control topic. In Study 2, participants watched either a surrealist film clip or a control film clip. In both studies, participants in the meaning-threat condition who had taken a placebo showed typical compensatory affirmations by becoming more punitive toward lawbreakers, whereas those who had taken acetaminophen, and those in the control conditions, did not.

Previous research has revealed differences in how people value and pursue positive affect in individualistic and collectivistic cultural contexts. Whereas Euro-Americans place greater value on high activation positive affect (HAP; e.g., excitement, enthusiasm, elation) than do Asian Americans and Hong Kong Chinese, the opposite is true for low activation positive affect (LAP; e.g., calmness, serenity, tranquility). Although the form of collectivism present in East Asia dictates that individuals control and subdue their emotional expressions so as to maintain harmonious relationships, the opposite norm emerges in Mexico and other Latin American countries, in that the cultural script of simpatía promotes harmony through the open and vibrant expression of positive emotion. Across two studies, we found that Mexicans display a pattern of HAP/LAP preference different from those from East Asian collectivistic cultures, endorsing HAP over LAP., (PsycINFO Database Record (c) 2012 APA, all rights reserved.)

In most societies, meat is valued more highly, yet tabooed more frequently, than any other type of food. Past research suggests that people avoid eating animals they consider similar to themselves, but what specific factors influence which they eat, and which they avoid? Across an array of samples from the USA, Canada, Hong Kong, and India, perceived animal intelligence and appearance emerged as the chief predictors of disgust at the thought of eating them. Furthermore, reflecting on animals' psychological attributes increased reported disgust, especially among Euro-Canadians and Euro-Americans, suggesting that these factors are more influential in shaping disgust in individualistic cultural contexts. Concordant with past research, disgust was a major predictor of willingness to eat animals, but social influence (frequency of consumption by friends and family) also emerged as a strong predictor, especially among Hong Kong Chinese and Indians, providing evidence that one's friends and family have a stronger influence on one's food choices in collectivistic cultural contexts., (Copyright © 2012 Elsevier Ltd. All rights reserved.)

Genetic essentialism is the tendency for people to think in more essentialist ways upon encountering genetic concepts. The current studies assessed whether genetic essentialist biases would also be evident at the automatic level. In two studies, using different versions of the Implicit Association Test, we found that participants were faster to categorize when genes and fate were linked, compared to when these two concepts were kept separate and opposing. In addition to the wealth of past findings of genetic essentialism with explicit and deliberative measures, these biases appear to be also evident with implicit measures.

In the target article (Dar-Nimrod & Heine, 2011), we provided a social-cognitive framework which identified genetic essentialist biases and their implications. In their commentaries, Haslam (2011) and Turkheimer (2011) indicated their general agreement with this framework but highlighted some important points for consideration. Haslam suggested that neuroessentialism is a comparable kind of essentialist bias and identified similarities with the genetic essentialism framework. In response, we acknowledge similarities but also identify qualitative and quantitative differences between genetic essentialism and other kinds of essentialist biases. Turkheimer challenged us to extend our discussion to address the question of how should people respond to genetic etiological information, critiqued the use of heritability coefficients, and identified a new construct (1 - rMZ), which may be termed a free-will coefficient. In response, we emphasize the need to transform interactionist explanations from being empty platitudes to becoming the default conceptual framework; we wholeheartedly accept his critical view of heritability coefficient estimates (but acknowledge a more limited utility for them); and we are intrigued by his conceptual interest in identifying free-will coefficients yet warn against falling into pitfalls similar to those that were stumbled into in the past., ((PsycINFO Database Record (c) 2011 APA, all rights reserved).)

This article introduces the notion of genetic essentialist biases: cognitive biases associated with essentialist thinking that are elicited when people encounter arguments that genes are relevant for a behavior, condition, or social group. Learning about genetic attributions for various human conditions leads to a particular set of thoughts regarding those conditions: they are more likely to be perceived as (a) immutable and determined, (b) having a specific etiology, (c) homogeneous and discrete, and (d) natural, which can lead to the naturalistic fallacy. There are rare cases of "strong genetic explanation" when such responses to genetic attributions may be appropriate; however, people tend to overweigh genetic attributions compared with competing attributions even in cases of "weak genetic explanation," which are far more common. The authors reviewed research on people's understanding of race, gender, sexual orientation, criminality, mental illness, and obesity through a genetic essentialism lens, highlighting attitudinal, cognitive, and behavioral changes that stem from consideration of genetic attributions as bases of these categories. Scientific and media portrayals of genetic discoveries are discussed with respect to genetic essentialism, as is the role that genetic essentialism has played (and continues to play) in various public policies, legislation, scientific endeavors, and ideological movements in recent history. Last, moderating factors and interventions to reduce the magnitude of genetic essentialism, which identify promising directions to explore in order to reduce these biases, are discussed., ((PsycINFO Database Record (c) 2011 APA, all rights reserved).)

Evolutionary psychology accounts of gender differences in sexual behaviors in general and men's sexual aggression, in particular, has been criticized for legitimizing males' sexual misconduct. To empirically assess such critiques, two studies examined how men's judgments of male sex crimes (solicitation of sex from a prostitute; rape) are influenced by exposure to (a) evolutionary psychological theories and (b) social-constructivist theories. Across two studies, a consistent pattern emerged compared with a control condition (a) exposure to evolutionary psychology theories had no observable impact on male judgments of men's criminal sexual behavior, whereas (b) exposure to social-constructivist theories did affect judgments, leading men to evaluate sex crimes more harshly. Additional results (from Study 2) indicate that this effect is mediated by perceptions of male control over sexual urges. These results have implications for journalists, educators, and scientists. Aggr. Behav. 37:440-449, 2011. © 2011 Wiley-Liss, Inc., (© 2011 Wiley-Liss, Inc.)

Much research has demonstrated that people perceive consumers of "good," low-fat foods as more moral, intelligent, and attractive, and perceive consumers of "bad," high-fat foods as less intelligent, less moral, and less attractive. Little research has contrasted perceptions of omnivores and vegetarians, particularly with respect to morality and gender characteristics. In two between-subject studies, we investigated people's perceptions of others who follow omnivorous and vegetarian diets, controlling for the perceived healthiness of the diets in question. In both studies, omnivorous and vegetarian participants rated vegetarian targets as more virtuous and less masculine than omnivorous targets., (Copyright © 2011 Elsevier Ltd. All rights reserved.)

Though recent adult immigrants often seem less acculturated to their new society than people who immigrated as children, it is not clear whether this difference is driven by duration of exposure or exposure during a sensitive developmental period. In a study aimed at disambiguating these influences, community and student samples of Hong Kong immigrants to Vancouver, Canada, completed the Vancouver Index of Acculturation, a measure that assesses respondents' identification with their mainstream and heritage cultures. A longer duration of exposure was found to be associated with greater identification with Canadian culture only at younger ages of immigration, but not at later ages of immigration. Conversely, identification with Chinese culture was unaffected by either age of immigration or length of exposure to Canadian culture. These findings provide evidence for a sensitive period for acculturation: People are better able to identify with a host culture the longer their exposure to it, but only if this exposure occurs when they are relatively young.

The endowment effect--the tendency for owners (potential sellers) to value objects more than potential buyers do--is among the most widely studied judgment and decision-making phenomena. However, the current research is the first to explore whether the effect varies across cultures. Given previously demonstrated cultural differences in self-construals and self-enhancement, we predicted a smaller endowment effect for East Asians compared with Westerners. Two studies involving buyers and sellers of a coffee mug (Study 1a) and a box of chocolates (Study 1b) supported this prediction. Study 2 conceptually replicated this cultural difference by experimentally manipulating independent and interdependent self-construals. Finally, Study 3 provided evidence for an underlying self-enhancement mechanism: Cultural differences emerged when self-object associations were made salient, but disappeared when self-object associations were minimized. Thus, the endowment effect may be influenced by the degree to which independence and self-enhancement (vs. interdependence and self-criticism) are culturally valued or normative.

Behavioral scientists routinely publish broad claims about human psychology and behavior in the world's top journals based on samples drawn entirely from Western, Educated, Industrialized, Rich, and Democratic (WEIRD) societies. Researchers - often implicitly - assume that either there is little variation across human populations, or that these "standard subjects" are as representative of the species as any other population. Are these assumptions justified? Here, our review of the comparative database from across the behavioral sciences suggests both that there is substantial variability in experimental results across populations and that WEIRD subjects are particularly unusual compared with the rest of the species - frequent outliers. The domains reviewed include visual perception, fairness, cooperation, spatial reasoning, categorization and inferential induction, moral reasoning, reasoning styles, self-concepts and related motivations, and the heritability of IQ. The findings suggest that members of WEIRD societies, including young children, are among the least representative populations one could find for generalizing about humans. Many of these findings involve domains that are associated with fundamental aspects of psychology, motivation, and behavior - hence, there are no obvious a priori grounds for claiming that a particular behavioral phenomenon is universal based on sampling from a single subpopulation. Overall, these empirical patterns suggests that we need to be less cavalier in addressing questions of human nature on the basis of data drawn from this particularly thin, and rather unusual, slice of humanity. We close by proposing ways to structurally re-organize the behavioral sciences to best tackle these challenges.

The meaning maintenance model asserts that following a meaning threat, people will affirm any meaning frameworks that are available. Three experiments tested (a) whether people affirm alternative meaning frameworks after reading absurdist literature, (b) what role expectations play in determining whether absurdities are threatening, and (c) whether people have a heightened need for meaning following exposure to absurdist art. In Study 1, participants who read an absurd Kafka parable affirmed an alternative meaning framework more than did those who read a meaningful parable. In Study 2, participants who read an absurd Monty Python parody engaged in compensatory affirmation efforts only if they were led to expect a conventional story. In Study 3, participants who were exposed to absurdist art or reminders of their mortality, compared to participants exposed to representational or abstract art, reported higher scores on the Personal Need for Structure scale, suggesting that they experienced a heightened need for meaning.

Humans are a cultural species, constantly navigating a complex web of culturally bound practices, norms, and worldviews. This article provides a brief overview of the relatively young field of cultural psychology, which investigates the many ways psychology and culture interweave with one another. Highlighting the cultural nature of the human species, it draws upon research on cultural evolution, enculturation, and developmental processes. This review further summarizes a number of cultural differences in how people perceive the self, and the behavioral consequences that follow from these differences, in the domains of internal and external attribution styles, motivations for self-enhancement, approach/avoidance, primary and secondary control, as well as motivations for distinctiveness and conformity. Additionally, the review discusses research on the intersection of culture and emotion, as well as cultural differences in cognition, perception, and reasoning. Copyright © 2010 John Wiley & Sons, Ltd. For further resources related to this article, please visit the WIREs website., (Copyright © 2010 John Wiley & Sons, Ltd.)