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The leaf-roller moth tribe Grapholitini comprises about 1200 described species and contains numerous notorious pests of fruits and seeds. The phylogeny of the tribe has been little studied using contemporary methods, and the monophyly of several genera remains questionable. In order to provide a more robust phylogenetic framework for the group, we conducted a multiple-gene phylogenetic analysis of 104 species representing 27 genera of Grapholitini and 29 outgroup species. Divergence time, ancestral area, and host plant usage were also inferred to explore evolutionary trends in the tribe. Our analyses indicate that Larisa and Corticivora, traditionally assigned to Grapholitini, are best excluded from the tribe. After removal of these two genera, the tribe is found to be monophyletic, represented by two major lineages—a Dichrorampha clade and a Cydia clade, the latter of which can be divided into seven generic groups. The genus Grapholita was found to be polyphyletic, comprising three different clades, and we propose three genera to accommodate these groups: Grapholita (sensu stricto), Aspila (formerly a subgenus of Grapholita) and Ephippiphora (formerly considered a synonym of Grapholita). We summarize each generic group, including related genera not included in our analysis, providing morphological, pheromone and food plant characters that support particular branches within the molecular hypotheses. Biogeographical analyses indicate that Grapholitini probably originated in the Nearctic, Afrotropical and Neotropical regions in the Lutetian of the middle Eocene (ca. 44.3 Ma). Our results also indicate that most groups in Grapholitini originated from Fabaceae-feeding monophagous or oligophagous ancestors, and that host plant shifts probably promoted species diversification within the tribe.

Reliable quantitative vegetation reconstructions for Europe during the Holocene are crucial to improving our understanding of landscape dynamics, making it possible to assess the past effects of environmental variables and land-use change on ecosystems and biodiversity, and mitigating their effects in the future. We present here the most spatially extensive and temporally continuous pollen-based reconstructions of plant cover in Europe (at a spatial resolution of 1° × 1°) over the Holocene (last 11.7 ka BP) using the ‘Regional Estimates of VEgetation Abundance from Large Sites’ (REVEALS) model. This study has three main aims. First, to present the most accurate and reliable generation of REVEALS reconstructions across Europe so far. This has been achieved by including a larger number of pollen records compared to former analyses, in particular from the Mediterranean area. Second, to discuss methodological issues in the quantification of past land cover by using alternative datasets of relative pollen productivities (RPPs), one of the key input parameters of REVEALS, to test model sensitivity. Finally, to validate our reconstructions with the global forest change dataset. The results suggest that the RPPs.st1 (31 taxa) dataset is best suited to producing regional vegetation cover estimates for Europe. These reconstructions offer a long-term perspective providing unique possibilities to explore spatial-temporal changes in past land cover and biodiversity.

Main research and development interest of 5G and beyond systems are focusing on solution for populated and hot spot areas, but public safety authorities need reliable communication solutions in rural and remote areas. Tactical bubbles—ad hoc-type nonpublic communications networks built with the 3rd Generation Partnership Project-based mobile technologies—offer mission critical communications services for public safety authorities in areas with bad mobile network coverage while also providing additional capacity in hot spot areas. In this experimental study, three interconnected bubbles acting on three different frequency bands—2.3 GHz (40), 2.6 GHz (7), and 3.5 GHz (n78)—are trialed. This article provides the analysis of different factors related to performance and user experience of tactical bubbles. Both ground-level and aerial trial measurements, as well as simulations, were utilized to verify our configuration for the tactical bubbles and their fulfillment of the quality requirements. The performance and coverage of the tactical bubbles are evaluated in a trial, which represents authorities’ search operations in a rural environment with hills, forests, and swamps. The achieved coverage range of the bubbles is more than 1000 m with the unmanned aerial system-based measurements, whereas by car, the coverage is less than 600 m. The effect of obstacles (ie, buildings and hills) on the coverage area and performance of bubbles is significant, especially on car-based measurements on the ground level.

The need for high-quality communications networks is urgent in data-based farming. A particular challenge is how to achieve reliable, cost-efficient, secure, and broadband last-mile data transfer to enable agricultural machine control. The trialed ad hoc private communications networks built and interconnected with different alternative wireless technologies, including 4G, 5G, satellite and tactical networks, provide interesting practical solutions for connectivity. A remotely controlled tractor is exemplified as a use case of machine control in the demonstrated private communication network. This paper describes the results of a comparative technology analysis and a field trial in a realistic environment. The study includes the practical implementation of video monitoring and the optimization of the control channel for remote-controlled unmanned agricultural tractors. The findings from this study verify and consolidate the requirements for network technologies and for cybersecurity enablers. They highlight insights into the suitability of different wireless technologies for smart farming and tractor scenarios and identify potential paths for future research.

We studied the evolutionary relationship of two widely distributed parapatric butterfly species, Melitaea athalia and Melitaea celadussa, using the ddRAD sequencing approach, as well as genital morphology and mtDNA data. M. athalia was retrieved as paraphyletic with respect to M. celadussa. Several cases of mito-nuclear discordance and morpho-genetic mismatch were found in the contact zone. A strongly diverged and marginally sympatric clade of M. athalia from the Balkans was revealed. An in-depth analysis of genomic structure detected high levels of admixture between M. athalia and M. celadussa at the contact zone, though not reaching the Balkan clade. The demographic modelling of populations supported the intermediate genetic make-up of European M. athalia populations with regards to M. celadussa and the Balkan clade. However, the dissimilarity matrix of genotype data (PCoA) suggested the Balkan lineage having a genetic component that is unrelated to the athalia-celadussa group. Although narrowly sympatric, almost no signs of gene flow were found between the main M. athalia group and the Balkan clade. We propose two possible scenarios on the historical evolution of our model taxa and the role of the last glacial maximum in shaping their current distribution. Finally, we discuss the complexities regarding the taxonomic delimitation of parapatric taxa.

In the present study, we aimed to specify the key competence domains perceived to be critical for the teaching profession and depict them as a comprehensive teacher competence model. An expert panel that included representatives from seven units providing university-based initial teacher education in Finland carried out this process. To produce an active construction of a shared understanding and an interpretation of the discourse in the field, the experts reviewed literature on teaching. The resulting teacher competence model, the multidimensional adapted process model of teaching (MAP), represents a collective conception of the relevant empirical literature and prevailing discourses on teaching. The MAP is based on Blömeke et al.’s, Zeitschrift für Psychologie, 223, 3–13, (2015) model which distinguishes among teacher competences (referring to effective performance of teachers’ work), competencies (knowledge, skills, and other individual competencies underlying and enabling effective teaching), and situation-specific skills of perceiving, interpreting, and making decisions in situations involving teaching and learning. The implications of the MAP for teacher education and student selection for initial teacher education are discussed.

Hypoxia-inducible factor (HIF), an αβ dimer, is the master regulator of oxygen homeostasis with hundreds of hypoxia-inducible target genes. Three HIF isoforms differing in the oxygen-sensitive α subunit exist in vertebrates. While HIF-1 and HIF-2 are known transcription activators, HIF-3 has been considered a negative regulator of the hypoxia response pathway. However, the human HIF3A mRNA is subject to complex alternative splicing. It was recently shown that the long HIF-3α variants can form αβ dimers that possess transactivation capacity. Here, we show that overexpression of the long HIF-3α2 variant induces the expression of a subset of genes, including the erythropoietin (EPO) gene, while simultaneous downregulation of all HIF-3α variants by siRNA targeting a shared HIF3A region leads to downregulation of EPO and additional genes. EPO mRNA and protein levels correlated with HIF3A silencing and HIF-3α2 overexpression. Chromatin immunoprecipitation analyses showed that HIF-3α2 binding associated with canonical hypoxia response elements in the promoter regions of EPO. Luciferase reporter assays showed that the identified HIF-3α2 chromatin-binding regions were sufficient to promote transcription by all three HIF-α isoforms. Based on these data, HIF-3α2 is a transcription activator that directly regulates EPO expression.

Shock-induced changes in ordinary chondrite meteorites related to impacts or planetary collisions are known to be capable of altering their optical properties. Thus, one can hypothesize that a significant portion of the ordinary chondrite material may be hidden within the observed dark C/X asteroid population. The exact pressure-temperature conditions of the shock-induced darkening are not well constrained. Thus, we experimentally investigate the gradual changes in the chondrite material optical properties as a function of the shock pressure. A spherical shock experiment with Chelyabinsk LL5 was performed in order to study the changes in its optical properties. The spherical shock experiment geometry allows for a gradual increase of shock pressure from $\sim$15 GPa at a rim toward hundreds of gigapascals in the center. Four distinct zones were observed with an increasing shock load. The optical changes are minimal up to $\sim$50 GPa. In the region of $\sim$50--60 GPa, shock darkening occurs due to the troilite melt infusion into silicates. This process abruptly ceases at pressures of $\sim$60 GPa due to an onset of silicate melting. At pressures higher than $\sim$150 GPa, recrystallization occurs and is associated with a second-stage shock darkening due to fine troilite-metal eutectic grains. The shock darkening affects the ultraviolet, visible, and near-infrared (UV, VIS, and NIR) region while changes to the MIR spectrum are minimal. Shock darkening is caused by two distinct mechanisms with characteristic pressure regions, which are separated by an interval where the darkening ceases. This implies a reduced amount of shock-darkened material produced during the asteroid collisions.

Hypoxia inactivates hypoxia‐inducible factor (HIF) prolyl 4‐hydroxylases (HIF‐P4Hs), which stabilize HIF and upregulate genes to restore tissue oxygenation. HIF‐P4Hs can also be inhibited by small molecules studied in clinical trials for renal anemia. Knowledge of systemic long‐term inactivation of HIF‐P4Hs is limited but crucial, since HIF overexpression is associated with cancers. We aimed to determine the effects of systemic genetic inhibition of the most abundant isoenzyme HIF prolyl 4‐hydroxylase‐2 (HIF‐P4H‐2)/PHD2/EglN1 on life span and tissue homeostasis in aged mice. Our data showed no difference between wild‐type and HIF‐P4H‐2‐deficient mice in the average age reached. There were several differences, however, in the primary causes of death and comorbidities, the HIF‐P4H‐2‐deficient mice having less inflammation, liver diseases, including cancer, and myocardial infarctions, and not developing anemia. No increased cancer incidence was observed due to HIF‐P4H‐2‐deficiency. These data suggest that chronic inactivation of HIF‐P4H‐2 is not harmful but rather improves the quality of life in senescence.

This work presents a multiple‐gene phylogenetic analysis of 70 species representing 24 genera of Cochylina and eight species representing eight genera of Euliina, and a maximum‐likelihood analysis based on 293 barcodes representing over 220 species of Cochylina. The results confirm the hypothesis that Cochylina is a monophyletic group embedded within a paraphyletic Euliina. Six major monophyletic lineages are recognized and defined within Cochylina: a Phtheochroa Group, a Henricus Group, an Aethes Group, a Saphenista Group, a Phalonidia Group and a Cochylis Group. The work summarizes the groups (including related genera not included in our analysis), provides morphological characters that support the molecular data, and compares results to previous phylogenies of Cochylina. The following nomenclatural changes are proposed: Brevicornutia, rev.stat.; Neocochylis, rev.stat.; Paracochylis, rev.stat.; Pontoturania, rev.stat.; and Platphalonidia, rev.stat.

The unmanned aircraft systems (UAS) are commonly used for aerial photography, express delivery for packets or video monitoring. The UAS technology can help responding to the growing demands of the frequency monitoring by national radiocommunication authorities (NRAs). This article presents a practical usage trial of an unmanned aircraft for monitoring the spectrum usage and finding the interference location. The monitoring of spectrum usage is one part of the NRAs’ process for guarantee user compliance with radio license conditions. Finding illegal or malfunctioning transmitters is a challenging task especially in urban environment, due to multi-path propagation conditions. This study focuses on practical assessment of the method for determining accurate location of radio frequency interference sources from the buildings using the UAS technology. The practicability of the UAS technology usage in spectrum monitoring activities was under the evaluation. This study showed promising results that UAS with the measurement equipment setup suits NRAs’ as a tool for mobile monitoring on the air.

Ustyurtiidae Kaila, Heikkilä & Nupponen, a new family of Urodoidea is introduced. The family is based on the genus Ustyurtia Kaila, Heikkilä & Nupponen, gen. n. The genus includes the type species U. zygophyllivora Kaila, Heikkilä & Nupponen, sp. n. and U. charynica Kaila, Heikkilä & Nupponen, sp. n., both from Kazakhstan. These two species, in particular the immature stages, have morphological attributes apomorphic of Urodoidea. The close affinity is also supported by DNA data based on several markers. We consider this new family warranted due to its sister group position to the remaining Urodoidea and a number of significant morphological differences in wing venation, male genitalia and the structure of the cocoon, apomorphic for Ustyurtiidae on the basis of an earlier published phylogeny. All other recognized genera of Urodoidea belong to the family Urodidae. The closest relatives and phylogenetic position of Urodoidea are not firmly established, but Urodoidea and Schreckensteinioidea have morphological similarities which, in the light of genetic analyses appear synapomorphic and possibly uniting these groups, rather than homoplasious as assumed earlier. The affinities of these superfamilies are discussed.

The internet-of-things (IoT) can change not only products but also companies’ whole business model. The goal of this article is to inform companies about the creation of business intelligence via utilisation of the industrial IoT. This review article assumes that combining business excellence, service design and knowledge gathering via data engineering into cognitive infocommunication (CogInfoCom) opens new possibilities to develop business ideas. These new possibilities are examined through a literature review on deployments of industrial IoT solutions. The survey responds to two research questions: what methods of knowledge gathering and business excellence are used in industrial IoT-based product, service and process innovations? How can knowledge gathering, business excellence and CogInfoCom methods be utilised to create added value in industrial IoT solutions? This survey reveals the richness and delicacy of these exploitation opportunities.

Technological development and increasing personal data collection and utilization raise the importance of understanding individuals’ privacy behavior. Privacy behavior denotes the willingness to disclose personal data for services utilizing these data. The literature presents various privacy behavior models (PBMs). However, the research is incoherent, with inconsistencies among models. Therefore, the application and subsequent development of PBMs are challenging. Different background theories are used for model construction, and studies have been conducted in distinct application domains. We studied whether the models’ inconsistencies could be explained by these differences. Our in-depth analysis of PBMs was based on a systematic literature review of the most often cited key studies. Our findings indicate that the choice of theories and the application domains do not explain inconsistencies; instead, the models are often of an ad hoc type and constructed in an eclectic way. These results imply the need for more consistent research on privacy behavior.

Commercial mobile operators’ networks will be used for public safety communications due to demand for wireless broadband services, new applications, and smart devices. Existing dedicated professional mobile radio networks, such as terrestrial trunked radio, Tetrapol, and project 25, are based on narrowband technologies and hence their data bandwidth is limited. This paper studies how critical communications needed, e.g., by ambulance personnel, rescue squads, and law enforcement agencies can be implemented over a 5G network. The most important technology enablers are described and test network architectures used in our project given. We focus on two different use cases. First, how to enable priority communications over a commercial mobile network. Second, how to create rapidly deployable networks for emergency and tactical operations. Tests done with the implemented systems in real networks show that both approaches are very promising for future critical users. Techniques such as network slicing and licensed shared access provide means to support mission critical applications in any environment.

Coptoformica Müller, 1923 is a subgenus of Formica Linnaeus, 1758 that consists of c. a dozen species of ants that typically inhabit open grassy habitats and build small nest mounds. The most recent addition to the group is Formica fennica Seifert, 2000. The description was based on morphological characters, but the species status has not been confirmed by molecular methods. In this study, we use thirteen DNA microsatellite markers and a partial mitochondrial COI gene sequence to assess the species status of F. fennica, by comparing the genetic variation among samples identified as F. fennica and six other boreal Formica (Coptoformica) species. Most of the species studied form separate, discontinuous clusters in phylogenetic and spatial analyses with only little intraspecific genetic variation. However, both nuclear and mitochondrial markers fail to separate the species pair F. exsecta Nylander, 1846 and F. fennica despite established morphological differences. The genetic variation within the F. exsecta/fennica group is extensive, but reflects spatial rather than morphological differences. Finnish F. fennica populations studied so far should not be considered a separate species, but merely a morph of F. exsecta.

This study presents the usage of unmanned aircraft for site survey and antenna pattern measurements in-situ in mobile networks. The site survey is one part of the verification and optimization process of the network operator. Mobile network operators’ responsibility is to ensure the broadly available, reliable network series that are essential for customer experience. Technically that means mobile network coverage of the desired area without holes and with good capacity. The operator performs network planning in order to optimize coverage and capacity. It is essential to know the antenna pattern to plan and design an effective and efficient mobile network. Antenna manufacturers provide antenna radiation patterns measured in the standard anechoic chamber. Understanding how real site implementation differs from the laboratory measurements is also crucial. This study focuses on developing in-situ antenna measurements for the antenna patterns recorded in the laboratory. The evaluation of antenna radiation patterns in a real environment will provide additional information about what kind of effect the antenna site mast, installation, and antenna performance parameters will have on the theoretical radiation pattern.

This paper has two main objectives. First, it describes the practical challenges of field trials and proposes a developed test method. Second, the test method is used to compare the uplink (UL) performance with different antenna technologies when user equipment (UE) does not have a line of sight (LOS) to the evolved node B. Both passive and active antenna configurations were used in the performance evaluation. Modern cellular networks have high demands for capacity, reliability, and availability. The verification of a network’s configuration and technological features is essential to guarantee network performance, and the performance of a network must be verified by the laboratory testing or field trials; such trials produce the experimental knowledge of technology features and configurations. Technological and environmental factors must also be considered before performing mobile network field testing. This paper showed that moving UE produces more reliable and repeatable results than measurements with stationary UE. Our antenna configuration comparison study revealed that in the UL direction, active antenna system beam control could significantly increase the UL capacity in non-LOS conditions.

Electroblowing was used to prepare ZnO and aluminum doped zinc oxide (AZO, 1–3 cation-% of Al) fibers. The as-blown fibers were calcined at 500 °C to obtain the target material. The average fiber diameters ranged from 240 ± 60 nm for ZnO fibers to 330 ± 80 nm for AZO with 3% Al. Smaller crystallite size was measured with x-ray diffraction for the Al doped fibers. Electroblowing was found out be an effective method to increase the fiber productivity over electrospinning and other methods reported in literature to prepare AZO fibers as a high production rate of 0.32 g/h was achieved. The ZnO and AZO fibers could be converted to zeolitic imidazole framework-8 [ZIF-8, zinc(2-methylimidazolate)₂] by a solvent free thermal treatment in an autoclave under 2-methylimidazole (HmIM) vapor at 150 and 200 °C while preserving the fibrous structure. The conversion process to ZIF-8 occurred faster at higher temperatures and on fibers with smaller crystallite size. Depending on the conversion treatment time either ZnO/ZIF-8 and AZO/ZIF-8 core/shell fibers or ZIF-8 fibers could be obtained. At best the prepared ZIF-8 fibers had a very high BET specific surface area of 1340 m²/g.

In this paper, we describe a spectrum access system (SAS) based Citizens Broadband Radio Service (CBRS) field trial using a live LTE network in the 3.5 GHz band. The latest WInnForum specification guided the implementation of the relevant protocols for SAS operation. Here, we evaluate the performance of a CBRS field trial by using one of the most important performance indicators in a spectrum sharing scenario — the evacuation time. It indicates how rapidly the secondary user relinquishes the shared spectrum band to the primary user. Following the applied protocols, we measure and analyze the time scales for the evacuation and frequency change procedures in a field trial environment. Our work shows that the set time limits for the protection of primary users against interference are realistic when using commercially available mobile networks and equipment. Finally, utilizing knowledge of the latest base station models, we propose ways to reduce the evacuation and reconfiguration time by up to 70%.

The aim of this paper is to introduce the measurement method for challenging Non-Line of Sight (NLOS) conditions in mobile networks. The need to develop the measurement method appeared LTE (Long Term Evolution) uplink (UL) performance with different antenna technologies when receiving NLOS signal in field tests. Many challenges appeared during the study. This paper introduces the challenges and presents solution.