1. Cyanines as New Fluorescent Probes for DNA Detection and Two-Photon Excited Bioimaging
- Author
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Man Shing Wong, Kok Wai Cheah, Heidi W C Leung, Frédéric Bolze, Nai Ki Mak, Jean-François Nicoud, King Fai Li, Daniel W. J. Kwong, Po Lam Wu, and Xin Jiang Feng
- Subjects
Photons ,Chemistry ,Carbazole ,Organic Chemistry ,Electron donor ,DNA ,Photochemistry ,Biochemistry ,Fluorescence ,chemistry.chemical_compound ,Microscopy, Fluorescence, Multiphoton ,Two-photon excitation microscopy ,Excited state ,Nitriles ,Microscopy ,Animals ,Cattle ,Physical and Theoretical Chemistry ,Cyanine ,Absorption (electromagnetic radiation) ,Fluorescent Dyes - Abstract
A series of cyanine fluorophores based on fused aromatics as an electron donor for DNA sensing and two-photon bioimaging were synthesized, among which the carbazole-based biscyanine exhibits high sensitivity and efficiency as a fluorescent light-up probe for dsDNA, which shows selective binding toward the AT-rich regions. The synergetic effect of the bischromophoric skeleton gives a several-fold enhancement in a two-photon absorption cross-section as well as a 25- to 100-fold enhancement in two-photon excited fluorescence upon dsDNA binding.
- Published
- 2010