22 results on '"Hedi Mighri"'
Search Results
2. Chemical Composition of Essential Oils from Eight Tunisian Eucalyptus Species and Their Antifungal and Herbicidal Activities
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Amira Ayed, Flavio Polito, Hedi Mighri, Mouna Souihi, Lucia Caputo, Lamia Hamrouni, Ismail Amri, Filomena Nazzaro, Vincenzo De Feo, Ann M. Hirsch, and Yassine Mabrouk
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Eucalyptus ,essential oils ,antifungal activity ,herbicidal activity ,Botany ,QK1-989 - Abstract
Eucalyptus species are known to produce metabolites such as essential oils (EOs) that play an important role in the control of weeds, pests and phytopathogenic fungi. The aims of this study were as follows: (i) to determine the chemical composition of the EOs derived from eight Eucalyptus species growing in Tunisia, and (ii) to study their possible antifungal and herbicidal activities. EOs were obtained by hydrodistillation from the dried leaves of eight Eucalyptus species, namely, E. angulosa, E. cladocalyx, E. diversicolor, E. microcoryx, E. ovata, E. resinifera, E. saligna and E. sargentii, and the determination of their composition was achieved by GC and GC-MS. The EOs’ antifungal activities were tested against four Fusarium strains, and the EOs’ herbicidal properties were evaluated on the germination and seedling growth of three annual weeds (Trifolium campestre, Lolium rigidum and Sinapis arvensis) and three cultivated crop species (Lepidium sativum, Raphanus sativus and Triticum durum). The EO yields ranged between 0.12 and 1.32%. The most abundant components found were eucalyptol, α-pinene, p-cymene, trans-pinocarveol, α-terpineol and globulol. All EOs showed significant antifungal activity against the four phytopathogenic Fusarium strains. E. cladocalyx EO exhibited the highest level of antifungal activity, and the greatest inhibition of seed germination was obtained even at lowest concentrations used. These findings suggested that E. resinifera, E. ovata and E. cladocalyx EOs could have applications in agriculture as possible biopesticides, as Fusarium antagonists and as bioherbicides.
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- 2023
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3. HPLC-MS Profiling, Antioxidant, Antimicrobial, Antidiabetic, and Cytotoxicity Activities of Arthrocnemum indicum (Willd.) Moq. Extracts
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Hafedh Hajlaoui, Soumaya Arraouadi, Hedi Mighri, Siwar Ghannay, Kaïss Aouadi, Mohd Adnan, Abdelbaset Mohamed Elasbali, Emira Noumi, Mejdi Snoussi, and Adel Kadri
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Arthrocnemum indicum extracts ,halophytes ,phytochemicals ,antimicrobial ,antioxidant ,antidiabetic ,Botany ,QK1-989 - Abstract
The purpose of this study was to evaluate for the first time the phytochemical constituents and biological properties of three (ethanol, acetone, and hexane) Arthrocnemum indicum (Willd.) Moq. (A. indicum) extracts. Quantitative analysis revealed the significantly (p < 0.05) dominance of ethanolic extract on total polyphenol (TPC; 303.67 ± 4.16 mg GAE/g DR) and flavonoid (TFC; 55.33 ± 2.52 mg CE/g DR) contents than the other extracts, also displaying high and equipotent condensed tannin (TCTC) contents as the acetone extract. The qualitative HPLC-MS analysis elucidates 19 and 18 compounds in ethanolic and acetonic extracts, respectively, belonging to the phenolics and flavonoids chemical classes. The extracts were also screened for their in vitro antioxidant activities using 1,1-diphenyl-2-picrylhydrazyl, superoxide anion, and ferric ion (Fe3+) reducing antioxidant power (FRAP), demonstrating the potent antioxidant activity of ethanolic extract, due to its stronger scavenging DPPH• (IC50 = 7.17 ± 1.26 μg/mL) which is not significantly (p > 0.05) different from the positive control, BHT (IC50 = 10.70 ± 0.61 μg/mL), however moderate activity through FRAP and superoxide anion radicals have been observed. Four Gram-positive, four Gram-negative bacteria, and four pathogenic fungi were used for the antimicrobial activity. In addition, S. epidermidis, M. luteus, E. faecalis, C. glabrata, C. parapsilosis, C. krusei were found to be the most susceptible strains towards ethanolic extract. Cytotoxicity values against human colon adenocarcinoma cells (HT29) and human epidermoid cancer cells (Hep2), and one continuous cell lineage control (Vero) revealed that the HT29 cancer cell line was the most responsive to A. indicum shoot extract treatment and significantly (p < 0.05) different from the other cancer cells. Moreover, when tested for their antidiabetic inhibitory effect, ethanol extract recorded the highest antidiabetic effect with IC50 = 13.17 ± 1.04 mg/mL, which is 8.4-fold higher than acetone extract. Therefore, the present study provides new findings on the use of A. indicum shoot ethanolic extract to cure many incurable diseases.
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- 2022
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4. Phytochemical Constituents and Antioxidant Activity of Oudneya Africana L. Leaves Extracts: Evaluation Effects on Fatty Acids and Proteins Oxidation of Beef Burger during Refrigerated Storage
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Hafedh Hajlaoui, Soumaya Arraouadi, Hedi Mighri, Mouna Chaaibia, Néji Gharsallah, Gaspar Ros, Gema Nieto, and Adel Kadri
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oudneya africana ,antioxidant activity ,hplc-ms phenolic analysis ,beef burgers ,tbars ,protein thiols groups ,sensory analysis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Five Oudneya Africana (OA) leaves extracts were screened for their total phenolic (TPC), total flavonoid (TFC), condensed tannins (CTC) content, as well as their antioxidant capacity. The highest amount of TPC (661.66 ± 0.08 mg GAE/g), TFC (344.68 ± 0.44 mg QE/g) and TCT (90.18 ± 0.49 mg CE/g) was recorded to ethanol, acetone, and dichloromethane extracts, respectively. For 2,2-diphenyl-1-picrylhydrazyl (DPPH) (22.00 ± 0.03 µg/mL) and Reducing Power Assay (FRAP) (269.00 ± 0.01µg/mL) assays, ethanol extract showed the potent activity, while with ABTS test, acetone extract was the most active (761.15 ± 0.09 µg/mL). HPLC-MS analysis of acetonic and ethanolic extracts reveals the predominance of quinic acid, chlorogenic acid, 4-O-caffeoylquinic acid, and rutin compounds. The addition effect evaluation of OA extracts in beef burger preservation demonstrates the powerful effect (p < 0.05) of acetonic and ethanolic ones (0.03%) to inhibit lipids oxidation during storage for 10 days, given by the lowest increase in Thiobarbituric Acid-reactive Substances (TBARS) values as compared to the (−) control with a significant difference between free thiols values. In addition, these two extracts appear to be effective (p < 0.05) for pH stability, color, and sensory parameters as compared to (+) and (−) controls and aqueous extract. Hamburger odour was considered as a dependent variable in multiple linear regression analysis, where the models results showed that physicochemical parameters determine more burger odour than sensorial ones.
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- 2019
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5. Artemisia campestris dried leaf extracts: Effects of different extraction methods and solvents on phenolic composition and biological activities
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Rafika Metoui, Hedi Mighri, Jalloul Bouajila, Mansour Znati, Hajer El-Jani, and Ahmed Akrout
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Plant Science - Published
- 2022
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6. Metabolite profiling and potential antioxidant activity of sixteen fennel (Foeniculum vulgare Mill.) populations growing wild in Tunisia
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Marwa Khammassi, Hedi Mighri, Manel Ben Mansour, Ismail Amri, Bassem Jamoussi, and Abdelhamid Khaldi
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Plant Science - Published
- 2022
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7. Phytochemical Screening of Essential Oils and Methanol Extract Constituents of Wild Foeniculum vulgare Mill.: a Potential Natural Source for Bioactive Molecules
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Marwa Khammassi, Kouki Habiba, Hedi Mighri, Souihi Mouna, Kochti Oumayma, Emine Seçer, Amri Ismail, Bassem Jamoussi, and Mabrouk Yassine
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Chemistry (miscellaneous) ,Environmental Chemistry ,Physical and Theoretical Chemistry ,Catalysis - Published
- 2023
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8. Antioxidant and Antimicrobial Activities of Thymus vulgaris L. Essential Oil Growing Wild in Tunisia
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Adel Kadri, Emira Noumi, Mejdi Snoussi, Kaïss Aouadi, Hedi Mighri, Soumaya Arraouadi, and Hafedh Hajlaoui
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The aim of this work is to investigate the chemical composition, antioxidant, and antimicrobial activities of Tunisian Thymus vulgaris essential oil (TVEO). DPPH, superoxide anion, reducing power, chelating effect on ferrous ions and β-Carotene assays have been employed to determine the antioxidant potential of TVEO. In contrast, 24 reference bacterial strains and 16 fungal strains have been used for the assessment of the antimicrobial activity. Results revealed that TVEO has as carvacrol (67.33%) chemotype, it was equipped with an important antioxidant capacity that is better (P
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- 2021
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9. Moringa oleifera leaves: could solvent and extraction method affect phenolic composition and bioactivities?
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Hedi Mighri, Mahmoud Mabrouk, Talel Bouhamda, Ozfer Yesilada, Naima Bennour, Ahmed Akrout, Elif Apohan, and Hasan Küçükbay
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0106 biological sciences ,Chromatography ,010405 organic chemistry ,Butanol ,Ethyl acetate ,General Medicine ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Solvent ,Moringa ,chemistry.chemical_compound ,chemistry ,Liquid chromatography–mass spectrometry ,010608 biotechnology ,Maceration (wine) ,Composition (visual arts) ,Biotechnology ,Dichloromethane - Abstract
This study was carried out to evaluate the effect of extraction methods (direct maceration (DM) and successive maceration (SM)) and solvents (dichloromethane (DCM), ethyl acetate (EAc), butanol (Bu...
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- 2021
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10. Characterization of Silybum marianum and Silybum eburneum seed oils: Phytochemical profiles and antioxidant properties supporting important nutritional interests.
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Samah Maaloul, Imen Ghzaiel, Maher Mahmoudi, Hédi Mighri, Vivien Pires, Anne Vejux, Lucie Martine, Jean-Paul Pais de Barros, Emmanuelle Prost-Camus, Fayçal Boughalleb, Gérard Lizard, and Raoudha Abdellaoui
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Medicine ,Science - Abstract
Milk thistle seed oil is still not a well-known edible oil. Silybum marianum (milk thistle), is present in several countries and is the only known representative of the genus Silybum. However, Silybum eburneum, which is an endemic plant in Spain, Kenya, Morocco, Algeria, and Tunisia, is considered a marginalized species. The present work is the first report that gives information on the lipid and phenolic profiles of Tunisian S. eburneum seed oil compared to those of Tunisian S. marianum seed oil. In addition, the antioxidant properties of these oils were determined with DPPH, FRAP, and KRL assays, and their ability to prevent oxidative stress was determined on human monocytic THP-1 cells. These oils are characterized by high amounts of unsaturated fatty acids; linoleic acid and oleic acid are the most abundant. Campesterol, sitosterol, stigmasterol, and β-amyrin were the major phytosterols identified. α-tocopherol was the predominant tocopherol found. These oils also contain significant amounts of phenolic compounds. The diversity and richness of Silybum marianum and Silybum eburneum seed oils in unsaturated fatty acids, phenolic compounds, and tocopherols are associated with high antioxidant activities revealed by the DPPH, FRAP, and KRL assays. In addition, on THP-1 cells, these oils powerfully reduced the oxidative stress induced by 7-ketocholesterol and 7β-hydroxycholesterol, two strongly pro-oxidant oxysterols often present at increased levels in patients with age-related diseases. Silybum marianum and Silybum eburneum seed oils are therefore important sources of bioactive molecules with nutritional interest that prevent age-related diseases, the frequency of which is increasing in all countries due to the length of life expectancy.
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- 2024
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11. Phytochemicals, antioxidant and antimicrobial potentials and LC-MS analysis of hydroalcoholic extracts of leaves and flowers of Erodium glaucophyllum collected from Tunisian Sahara
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José Maria Ross-Garcia, Adel Kadri, Amal Daoud, Hafedh Hajlaoui, Hedi Mighri, Neji Gharsallah, and Sana Bakari
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Antioxidant ,medicine.medical_treatment ,Hyperoside ,lcsh:TX341-641 ,antioxidant and antimicrobial activities ,01 natural sciences ,chemistry.chemical_compound ,Rutin ,0404 agricultural biotechnology ,lcsh:Technology (General) ,medicine ,Gallic acid ,Erodium glaucophyllum hydroalcoholic extracts ,Naringin ,Traditional medicine ,010401 analytical chemistry ,food and beverages ,04 agricultural and veterinary sciences ,Quinic acid ,Kill-time ,Antimicrobial ,040401 food science ,LC/MS analysis ,0104 chemical sciences ,chemistry ,Phytochemical ,lcsh:T1-995 ,lcsh:Nutrition. Foods and food supply ,Food Science ,Biotechnology - Abstract
Erodium glaucophyllum collected from Tunisian Sahara was never presented in the relevant literature yet. In this study, the phytochemical profiles, chemical compositions, antioxidant and antimicrobial activities, and Kill-time assay of the hydroalcoholic extract of flowers and leaves were investigated. The highest content in phenols, flavonoids and tannins was shown in flowers extract (350 ± 5 mg GAE/g, 34 ± 1 mg QE/g and 221.8 ± 0.0 mg β-carotene E/g, respectively). The carotenoids amount in leaves was about 6-fold higher than it’s in flowers. Conducted LC/MS analysis revealed the presence of 17 compounds in flowers and leaves extract with the main were quinic acid and naringin followed by rutin, hyperoside, gallic acid and Apegenin-7-o-glucoside. Flowers and leaves extracts showed a powerful antimicrobial effect against all strains and the most observed effect was shown with Gram negative bacteria. Time kill kinetics endorses the effectiveness of both extracts to possess bactericidal effect at a concentration less than 2 MIC. Results of both organs open a new promising way for the development of antioxidant and antimicrobial agents for food preservatives against food deterioration.
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- 2018
12. Chemical Composition and Antimicrobial Activity of Pituranthos chloranthus (Benth.) Hook and Pituranthos tortu-osus (Coss.) Maire Essential Oils from Southern Tunisia
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Ahmed Akrout, Khawla Sabri, Hajer El-jeni, Mohamed Neffati, and Hedi Mighri
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Limonene ,food.ingredient ,Sabinene ,Antimicrobial ,law.invention ,chemistry.chemical_compound ,food ,chemistry ,law ,Myrcene ,Herb ,Botany ,Pharmacology (medical) ,Antibacterial activity ,Chemical composition ,Essential oil - Abstract
Essential oils (EO) from fresh and dry aerial parts of Pituranthos chloranthus (Benth.) Hook and Pituranthos tortuosus (Coss.) Maire were isolated by hydrodistillation and analyzed by GC/MS. The main constituents of the EO obtained from fresh herb of P. chloranthus were found to be α-pinene, sabinene, cis-ocimene and myrcene. In dry biomass, a significant increase of the content of some compounds such as α-phellandrene, △,3-carene and β-phellandrene characterized the oil. Minor changes in the chemical composition of the P. tortuosus EOs obtained from fresh or dry herbs and the major constituents were found to be sabinene and myrcene with equilibrate amounts of α-pinene, p-cymene, cis-ocimene, limonene, trans-β-ocimene, γ-terpinene and cis-verbenol. The paper disc diffusion method was used to evaluate the antibacterial activity and results showed an important inhibitory effect of oils obtained from fresh herb against most tested bacteria.
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- 2015
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13. Tunisian Silybum Species: Important Sources of Polyphenols, Organic Acids, Minerals, and Proteins across Various Plant Organs
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Samah Maaloul, Maher Mahmoudi, Hédi Mighri, Imen Ghzaiel, Talel Bouhamda, Fayçal Boughalleb, Adil El Midaoui, Anne Vejux, Gérard Lizard, and Raoudha Abdellaoui
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Silybum marianum ,Silybum eburneum ,minerals ,polyphenols ,organic acids ,storage proteins ,Botany ,QK1-989 - Abstract
Silybum marianum and Silybum eburneum are wild edible Mediterranean plants used in the human diet. This study presents the initial findings on the phytochemical characterization of Tunisian S. marianum and S. eburneum organs. It examined their mineral, sugar, organic acid, polyphenolic, and seed storage protein contents, as well as their antioxidant potential. In S. marianum, stems had high sodium and potassium contents, while the immature and mature seeds were rich in calcium and magnesium. However, S. eburneum had high potassium levels in stems and high sodium and calcium levels in the flowers. S. marianum showed substantial fructose variation among its organs. Conversely, S. eburneum exhibited significant heterogeneity in glucose, sucrose, and maltose levels across its organs, with maltose exclusively detected in the immature seeds. A notable organ-dependent distribution of organic acids was observed among the two species. Higher levels of phenolic contents were detected in both mature and immature seeds in both species compared to the other plant parts. The seeds possessed higher antioxidant activities than other plant organs. In both S. marianum and S. eburneum seeds, albumins and globulins were the predominant protein fractions. This study brings evidence supporting the important potential of Silybum organs as sources of nutrients with antioxidant properties for producing functional food.
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- 2024
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14. Chemical composition and in vitro evaluation of antioxidant, antimicrobial, cytotoxicity and anti-acetylcholinesterase properties of Tunisian Origanum majorana L. essential oil
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Adel Kadri, Mahjoub Aouni, Hedi Mighri, Neji Gharsallah, and Hafedh Hajlaoui
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Antioxidant ,Cell Survival ,medicine.medical_treatment ,Phytochemicals ,Antineoplastic Agents ,Microbial Sensitivity Tests ,01 natural sciences ,Microbiology ,Antioxidants ,law.invention ,Inhibitory Concentration 50 ,Anti-Infective Agents ,beta-Carotene ,law ,Cell Line, Tumor ,Origanum ,medicine ,Oils, Volatile ,Animals ,Humans ,Food science ,Cytotoxicity ,Chemical composition ,Essential oil ,Microbial Viability ,biology ,Bacteria ,010405 organic chemistry ,Chemistry ,Fungi ,biology.organism_classification ,Antimicrobial ,beta Carotene ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Infectious Diseases ,Acetylcholinesterase ,Oxidation-Reduction - Abstract
This study investigated the chemical composition and evaluated the antioxidant, antimicrobial, cytotoxic and anti-acetylcholinesterase properties of Tunisian Origanum majorana essential oil. The findings showed that the oil exhibited high activity, particularly in terms of reducing power and β-Carotene bleaching, inducing higher IC50 values than BHT. The oil showed an important antimicrobial activity against 25 bacterial and fungal strains. In fact, the IZ, MIC and MBC values recorded for the bacterial strains were in the range of 8 ± 0-18.33 ± 0.57 mm, 0.097-3.125 and 0.39-6.25 mg/mL, respectively. The IZ, MIC and MFC values of the fungal strains varied between 11±0-28 ± 0 mm, 0.058-0.468 mg/mL and 0.234-1.875 mg/mL, respectively. A low cytotoxic effect was observed against cancer (Hep-2 and HT29) and continuous cell lineage (Vero), with CC50 values ranging from 13.73 to 85.63 mg/mL. The oil was also evaluated for anti-acetylcholinesterase effects, which showed that it exhibited significant activity with IC50 values reaching 150.33 ± 2.02 μg/mL.
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- 2016
15. Chemical Composition and Antioxidant Activity of Aqueous Extracts of Some Medicinal Plants Wild Growing in Southern Tunisia
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Hamida Turki, Ahmed Akrout, Mabrouka Krid, Hajer El-Jani, Hedi Mighri, Fatma Thabet, and Mohammed Neffati
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Aqueous solution ,Antioxidant ,Traditional medicine ,medicine.medical_treatment ,Botany ,medicine ,Biology ,Medicinal plants ,Chemical composition - Published
- 2012
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16. Antimicrobial and antioxidant activities of Artemisia herba-alba essential oil cultivated in Tunisian arid zone
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Hanen Najjaa, Mohamed Neffati, Hafedh Hajlaoui, Ahmed Akrout, and Hedi Mighri
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Antioxidant ,Traditional medicine ,Artemisia herba-alba ,General Chemical Engineering ,medicine.medical_treatment ,General Chemistry ,Biology ,Asteraceae ,Antimicrobial ,biology.organism_classification ,law.invention ,Camphor ,chemistry.chemical_compound ,chemistry ,law ,medicine ,Artemisia ,Essential oil ,Arid zone - Abstract
This study was conceived to examine the antimicrobial and antioxidant activities of four essential oil types extracted by hydrodistillation from the aerial parts of Artemisia herba-alba cultivated in southern Tunisia. The chemical composition was investigated using both capillary GC and GC/MS techniques. β-thujone, α-thujone, α-thujone/β-thujone and 1,8-cineole/camphor/α-thujone/β-thujone were respectively, the major components of these oil types. The antimicrobial activity of different oils was tested using the diffusion method and by determining the inhibition zone. The results showed that all examined oil types had great potential of antimicrobial activity against strains. In addition, antioxidant capacity was assessed by different in vitro tests and weak activity was found for these A. herba-alba oils.
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- 2010
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17. Chemical and Biological Characteristics of Essential Oil ofRosmarinus officinalisCultivated in Djerba
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Hanene Najjaa, Hedi Mighri, Slah Zaidi, Mohamed Neffati, Hafedh Hajlaoui, Hajer El Jani, and Ahmed Akrout
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alpha-Pinene ,biology ,Chemotype ,Organic Chemistry ,biology.organism_classification ,Biochemistry ,Rosmarinus ,Analytical Chemistry ,Borneol ,law.invention ,chemistry.chemical_compound ,Camphor ,chemistry ,law ,Officinalis ,Botany ,Camphene ,Food science ,Essential oil - Abstract
The essential oils extracted by Clavenger apparatus from leaves of Rosmarinus officinalis cultivated in different areas in Djerba (Island in the southern of Tunisia) were evaluated for their chemical composition (GC and GC-MS methods), antioxidant (DPPH method) and antibacterial activities (agar-well diffusion method). GC-MS analysis revealed the presence of three chemotypes of oils: 1,8-cineole, 1,8-cineole/camphor/α-pinene/camphene and 1,8-cineole/camphor/α-pinene/ verbenone/borneol which was not previously detected in Tunisian Rosmarinus officinalis. The three chemtypes exhibited moderate antioxidant activity with an IC50 ranged from 4.186 mg/ml for chemotype I to 7.298 for chemotype II and showed strong to moderate antibacterial activity against the six bacterial strains tested with a MIC ranged from 0.156 to 1.25 mg/mL (Chemotype I and chemotype II) and from 1.25 to 5 mg/mL (chemotype III). This study showed that the essential oil extracted from Rosmarinus officinalis cultivated in Djerba ha...
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- 2010
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18. The Essential Oil FromArtemisia herba-albaAsso Cultivated in Arid Land (South Tunisia)
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Hedi Mighri, Félix Tomi, Mohamed Neffati, Ahmed Akrout, and Joseph Casanova
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biology ,Artemisia herba-alba ,Vegetable material ,General Chemistry ,Asteraceae ,biology.organism_classification ,Arid ,law.invention ,Camphor ,chemistry.chemical_compound ,Agronomy ,chemistry ,law ,Artemisia ,Chemical composition ,Essential oil - Abstract
Seedlings of Artemisia herba-alba Asso collected from Kirchaou area were transplanted in an experimental garden near the Institut des Regions Arides of Medenine (Tunisia). During three years, the aerial parts were harvested (three levels of cutting, 25%, 50%, and 75% of the plant), at full blossom and during the vegetative stage. The essential oil was isolated by hydrodistillation and its chemical composition was determined by GC (RI) and 13C-NMR. With respect to the quantity of vegetable material and the yield of hydrodistillation, it appears that the best results were obtained for plants cut at 50% of their height and during the full blossom. The chemical composition of the essential oil was dominated by b-thujone, a-thujone, 1,8-cineole, camphor and trans-sabinyl acetate, irrespective of the level of cutting and the period of harvest. It remains similar to htat of plants growing wild in the same area.
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- 2009
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19. IMPACT OF SEASON AND HARVEST FREQUENCY ON BIOMASS AND ESSENTIAL OIL YIELDS OF ARTEMISIA HERBA-ALBA CULTIVATED IN SOUTHERN TUNISIA
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Joseph Casanova, Mohamed Neffati, Hedi Mighri, Ahmed Akrout, and Félix Tomi
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Agronomy ,biology ,law ,Artemisia herba-alba ,fungi ,Botany ,food and beverages ,Biomass ,biology.organism_classification ,Agronomy and Crop Science ,Essential oil ,law.invention - Abstract
SUMMARYArtemisia herba-alba Asso has been successfully cultivated in the Tunisian arid zone. However, information regarding the effects of the harvest frequency on its biomass and essential oil yields is very limited. In this study, the effects of three different frequencies of harvesting the upper half of the A. herba-alba plant tuft were compared. The harvest treatments were: harvesting the same individual plants at the flowering stage annually; harvesting the same individual plants at the full vegetative growth stage annually and harvesting the same individual plants every six months. Statistical analyses indicated that all properties studied were affected by the harvest frequency. Essential oil yield, depended both on the dry biomass and its essential oil content, and was significantly higher from plants harvested annually at the flowering stage than the other two treatments. The composition of the β- and α-thujone-rich oils did not vary throughout the experimental period.
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- 2009
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20. Composition and intraspecific chemical variability of the essential oil from Artemisia herba-alba growing wild in a Tunisian arid zone
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Mohamed Neffati, Hedi Mighri, Félix Tomi, Slah Zaidi, Hajer El-jeni, Joseph Casanova, and Ahmed Akrout
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Tunisia ,Bioengineering ,Biochemistry ,Gas Chromatography-Mass Spectrometry ,Borneol ,law.invention ,chemistry.chemical_compound ,Camphor ,law ,Botany ,Oils, Volatile ,Molecular Biology ,Essential oil ,Bicyclic Monoterpenes ,Principal Component Analysis ,biology ,Chemotype ,Chemistry ,Artemisia herba-alba ,General Chemistry ,General Medicine ,Plant Components, Aerial ,biology.organism_classification ,Arid ,Artemisia ,Monoterpenes ,Molecular Medicine ,Chrysanthenone - Abstract
The intraspecific chemical variability of essential oils (50 samples) isolated from the aerial parts of Artemisia herba-alba Asso growing wild in the arid zone of Southeastern Tunisia was investigated. Analysis by GC (RI) and GC/MS allowed the identification of 54 essential oil components. The main compounds were β-thujone and α-thujone, followed by 1,8-cineole, camphor, chrysanthenone, trans-sabinyl acetate, trans-pinocarveol, and borneol. Chemometric analysis (k-means clustering and PCA) led to the partitioning into three groups. The composition of two thirds of the samples was dominated by α-thujone or β-thujone. Therefore, it could be expected that wild plants of A. herba-alba randomly harvested in the area of Kirchaou and transplanted by local farmers for the cultivation in arid zones of Southern Tunisia produce an essential oil belonging to the α-thujone/β-thujone chemotype and containing also 1,8-cineole, camphor, and trans-sabinyl acetate at appreciable amounts.
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- 2010
21. Chemical composition and biological activities of Tunisian Cuminum cyminum L. essential oil: a high effectiveness against Vibrio spp. strains
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Hafedh Hajlaoui, Emira Noumi, Mejdi Snoussi, Riadh Ksouri, Najla Trabelsi, Hedi Mighri, and Amina Bakhrouf
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Cuminum ,Tunisia ,Microbial Sensitivity Tests ,Biology ,Toxicology ,Cymenes ,Antioxidants ,Gas Chromatography-Mass Spectrometry ,law.invention ,Linoleic Acid ,chemistry.chemical_compound ,Nutraceutical ,Picrates ,beta-Carotene ,law ,Botany ,Oils, Volatile ,Animals ,Humans ,Seawater ,Food science ,Essential oil ,Vibrio ,beta-Pinene ,Biphenyl Compounds ,Food preservation ,Fishes ,General Medicine ,biology.organism_classification ,Antimicrobial ,Oxidants ,beta Carotene ,Anti-Bacterial Agents ,chemistry ,Seeds ,Food Microbiology ,Oxidation-Reduction ,Food Science - Abstract
Essential oil extracted by hydrodistillation from Tunisian variety of Cuminumcyminum was characterized by means of GC and GC-MS. Twenty-one components were identified and C. cyminum contained cuminlaldehyde (39.48%), gamma-terpinene (15.21%), O-cymene (11.82%), beta-pinene (11.13%), 2-caren-10-al (7.93%), trans-carveol (4.49%) and myrtenal (3.5%) as a major components. Moreover, C. cyminum oil exhibited higher antibacterial and antifungal activities with a high effectiveness against Vibrio spp. strains with a diameter of inhibition zones growth ranging from 11 to 23 mm and MIC and MBC values ranging from (0.078-0.31 mg/ml) to (0.31-1.25mg/ml), respectively. On the other hand, the cumin oil was investigated for its antioxidant activities using four different tests then compared with BHT. Results showed that cumin oil exhibit a higher activity in each antioxidant system with a special attention for beta-carotene bleaching test (IC(50): 20 microg/ml) and reducing power (EC(50): 11 microg/ml). In the light of these findings, we suggested that C. cyminum essential oil may be considered as an interesting source of antibacterial, antifungal and antioxidants components used as potent agents in food preservation and for therapeutic or nutraceutical industries.
- Published
- 2010
22. Influence of drying time and process on Artemisia herba-alba Asso essential oil yield and composition
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Félix Tomi, Mohamed Neffati, Ahmed Akrout, Hedi Mighri, Joseph Casanova, Ressources naturelles, Sciences pour l'environnement (SPE), Centre National de la Recherche Scientifique (CNRS)-Université Pascal Paoli (UPP)-Centre National de la Recherche Scientifique (CNRS)-Université Pascal Paoli (UPP), and Lugrezi, Cathy
- Subjects
[CHIM.ANAL] Chemical Sciences/Analytical chemistry ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,law.invention ,Borneol ,chemistry.chemical_compound ,Camphor ,0404 agricultural biotechnology ,law ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,Food science ,Chemical composition ,Essential oil ,ComputingMilieux_MISCELLANEOUS ,Chromatography ,biology ,Artemisia herba-alba ,010401 analytical chemistry ,Organic Chemistry ,04 agricultural and veterinary sciences ,biology.organism_classification ,040401 food science ,0104 chemical sciences ,chemistry ,Artemisia ,Chrysanthenone ,Composition (visual arts) - Abstract
The essential oil content of Artemisia herba-alba Asso decreased along the drying period from 2.5 % to 1.8 %. Conversely, the composition of the essential oil was not qualitatively affected by the drying process. The same principle components were found in all essential analyzed such as α-thujone (13.0 – 22.7 %), β-thujone (18.0 – 25.0 %), camphor (8.6 - 13 %), 1,8-cineole (7.1 – 9.4 %), chrysanthenone (6.7 – 10.9 %), terpinen-4-ol (3.4 – 4.7 %). Quantitatively, during the air-drying process, the content of some components decreased slightly such as α-thujone (from 22.7 to 15.9 %) and 1,8-cineole (from 9.4 to 7.1 %), while the amount of other compounds increased such as chrysanthenone (from 6.7 to 10.9 %), borneol (from 0.8 to 1.5 %), germacrene-D (from 1.0 to 2.4 %) and spathulenol (from 0.8 to 1.5 %). The chemical composition of the oil was more affected by oven-drying the plant material at 35°C. α-Thujone and β-thujone decreased to 13.0 %and 18.0 %respectively, while the percentage of camphor,...
- Published
- 2009
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