Law Sui-Lin., Thesis (M.Phil.)--Chinese University of Hong Kong, 2003., Includes bibliographical references (leaves 99-109)., s in English and Chinese., CONTENTS --- p.i, p.v, 撮要 --- p.vii, ACKNOWLEDGEMENTS --- p.ix, LIST OF FIGURES & TABLES --- p.xi, ABBREVIATIONS --- p.xv, Chapter Chapter 1 --- INTRODUCTION --- p.1, Chapter 1.1 --- The Transition of Hyperplastic to Hypertrophic Growth During Heart Development --- p.1, Chapter 1.2 --- The Controversial Capability of Heart Regeneration --- p.3, Chapter 1.3 --- Challenges in Treating Heart Diseases --- p.5, Chapter 1.4 --- A New Insight Behind Traditional Chinese Medicine (TCM) for Treating Heart Diseases --- p.7, Chapter 1.5 --- The Potential Mitogenic TCMs on Cardiomyocytes --- p.10, Chapter 1.5.1 --- Radix Astragali --- p.11, Chapter 1.5.2 --- Radix Ophiopogonis --- p.12, Chapter Chapter 2 --- MATERIALS & METHODS --- p.14, Chapter 2.1 --- Materials --- p.14, Chapter 2.2 --- Cell Culture --- p.16, Chapter 2.2.1 --- Primary neonatal rat cardiomyocytes cell culture --- p.16, Chapter 2.2.1.1 --- Mayer's hemalum-eosin staining --- p.17, Chapter 2.2.2 --- Primary rat fibroblasts cell culture --- p.18, Chapter 2.2.3 --- H9C2 cardiac cell culture --- p.18, Chapter 2.3 --- TCMs Preparation and Treatment --- p.19, Chapter 2.3.1 --- Preparation of TCMs powder from aqueous extracts --- p.19, Chapter 2.3.2 --- Preparation of culture medium with TCMs powder --- p.19, Chapter 2.3.3 --- Pre-treatment of undifferentiated and differentiated H9C2 cardiac cells with TCMs --- p.20, Chapter 2.3.4 --- Post-treatment of differentiated H9C2 cardiac cells with TCMs --- p.20, Chapter 2.4 --- Assessment of DNA Synthesis and Proliferation --- p.21, Chapter 2.4.1 --- Tritiated thymidine incorporation assay --- p.21, Chapter 2.4.2 --- 5-Bromo-2'-deoxy-uridine (BrdU) assay --- p.22, Chapter 2.4.3 --- Cell counting --- p.23, Chapter 2.4.4 --- Statistical analysis --- p.23, Chapter 2.5 --- Screening of Differentially Expressed Genes in H9C2 Cells after TCM Treatment by cDNA Microarray --- p.25, Chapter 2.5.1 --- Total RNA extraction --- p.25, Chapter 2.5.2 --- RNA labeling --- p.26, Chapter 2.5.2.1 --- Synthesis of fluorescence labeled probe --- p.26, Chapter 2.5.2.2 --- Purification of fluorescence labeled probe --- p.27, Chapter 2.5.3 --- Microarray hybridization --- p.28, Chapter 2.5.3.1 --- Concentration of fluorescence labeled probe --- p.28, Chapter 2.5.3.2 --- Hybridization --- p.28, Chapter 2.5.3.3 --- Post-hybridization treatment --- p.29, Chapter 2.5.4 --- Data collection --- p.29, Chapter 2.5.4.1 --- Scanning of slide --- p.29, Chapter 2.5.4.2 --- Image processing: spots finding and quantification --- p.30, Chapter 2.5.5 --- Data normalization and analysis --- p.30, Chapter 2.6 --- Confirmation of Differentially Expressed Genes in H9C2 Cells after TCM Treatment by RT-PCR --- p.32, Chapter 2.6.1 --- DNase I digestion of total RNA sample --- p.32, Chapter 2.6.2 --- First-strand cDNA synthesis --- p.32, Chapter 2.6.3 --- RT-PCR of the candidate genes --- p.33, Chapter Chapter 3 --- RESULTS --- p.36, Chapter 3.1 --- Neonatal Primary Rat Cardiomyocytes --- p.36, Chapter 3.1.1 --- Preparation of high-purity neonatal primary rat cardiomyocytes --- p.36, Chapter 3.1.2 --- Neonatal primary rat cardiomyocytes ceased to undergo DNA replication after 6-day in vitro culturing --- p.38, Chapter 3.1.3 --- Both MD and HQ promoted the growth of day 1 primary rat cardiomyocytes in dose- and time-dependent manners --- p.40, Chapter 3.1.4 --- HQ is more potent than MD in promoting the growth of day 7 primary rat cardiomyocytes --- p.43, Chapter 3.2 --- H9C2 Cardiac cells --- p.45, Chapter 3.2.1 --- Proliferative effect of MD and HQ on undifferentiated H9C2 cardiac cells --- p.45, Chapter 3.2.2 --- Pre-treatment of HQ on H9C2 cardiac cells during differentiation --- p.50, Chapter 3.2.3 --- Pre-treatment of MD and HQ on differentiated H9C2 cardiac cells --- p.52, Chapter 3.2.4 --- Post-treatment of MD on differentiated H9C2 cardiac cells…… --- p.55, Chapter 3.3 --- Primary Rat Fibroblasts --- p.57, Chapter 3.3.1 --- Proliferative effect of MD and HQ on primary rat fibroblasts --- p.58, Chapter 3.4 --- Screening of Differentially Expressed Genes in H9C2 Cells after HQ Treatment by cDNA Microarray --- p.60, Chapter 3.4.1 --- Differentially expressed genes in undifferentiated H9C2 cardiac cells after HQ treatment --- p.60, Chapter 3.4.2 --- Differentially expressed genes in differentiated H9C2 cardiac cells after HQ treatment --- p.66, Chapter 3.4.3 --- Comparison of differentially expressed genes in both undifferentiated and differentiated H9C2 cardiac cells after HQ treatment --- p.72, Chapter 3.5 --- Confirmation of Differentially Expressed Genes in H9C2 Cells after HQ Treatment by RT-PCR --- p.73, Chapter 3.5.1 --- "Preferential up-regulation of N-G, N-G-dimethylarginine dimethylaminohydrolase mRNA expression level in undifferentiated H9C2 cardiac cells after HQ treatment " --- p.74, Chapter 3.5.2 --- Preferential up-regulation of heme oxygenase-3 mRNA expression level in undifferentiated H9C2 cardiac cells after HQ treatment --- p.75, Chapter 3.5.3 --- Preferential up-regulation of cyclin B mRNA expression level in differentiated H9C2 cardiac cells after HQ treatment --- p.76, Chapter Chapter 4 --- DISCUSSION --- p.77, Chapter 4.1 --- HQ Being a More Effective Mitogenic TCM than MD on Cardiomyocytes Exerted its Effect in Dose- and Time Dependent --- p.79, Chapter 4.2 --- Mitogenic Effect of Both MD and HQ might Possibly Due to the Regulation of Intrinsic Factors --- p.82, Chapter 4.3 --- HQ Rather Than MD Showed a Higher Specificity in Promoting DNA Synthesis in Cardiomyocytes --- p.83, Chapter 4.4 --- The Differentially Expressed Genes were Supported by The Clinical Functions of HQ --- p.85, Chapter 4.5 --- Relating the Differentially Expressed Genes with Cardiac Growth and Development --- p.87, Chapter 4.6 --- The Hypothetic Mechanisms of Action that HQ Exerted on Cardiac Growth and Development --- p.92, Chapter 4.7 --- Future Prospect --- p.94, Chapter 4.7.1 --- In vivo study of HQ on the proliferation of rat cardiomyocytes from neonatal to postnatal development --- p.94, Chapter 4.7.2 --- The study of transgenic mice carrying the target gene regulated by HQ on cardiac growth and development --- p.96, Chapter 4.7.3 --- The determination of active component of HQ on cardiac growth and development --- p.97, REFERENCES --- p.99, APPENDIX --- p.110, http://library.cuhk.edu.hk/record=b5891630, Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)