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A heavy-chain antibody (VHH) library against procymidone (PRM) was constructed via immunizing Bactrian camels. Through careful biopanning, seven nanobodies (Nbs) with different sequences were obtained. The variability in their performance was primarily attributed to the amino acid differences in complementarity-determining region 3 (CDR3), as analyzed by molecular docking. The Nb exhibiting the highest sensitivity, named NbFM5, was biotinylated and conjugated to streptavidin-labeled gold nanoparticles to preserve the epitope's activity and prevent a decrease in sensitivity due to traditional random electrostatic adsorption. Subsequently, a simple and sensitive immunochromatographic assay (ICA) was developed for rapid detection of PRM based on biotinylated Nb (btNb). The developed btNb-ICA showed a cut-off value of 200 ng/mL for visual judgment and a half-inhibitory concentration (IC50) of 6.04 ng/mL for quantitative detection. The limit of detection (LOD) was as low as 0.88 ng/mL. The recoveries in actual samples of crops ranged from 82.2 to 117.3%, aligning well with the results obtained from GC-MS/MS (R2 = 0.995). In summary, the developed btNb-ICA demonstrated high specificity and good accuracy for the rapid detection of PRM residues in vegetables. The total analysis time from preparing the sample to obtaining the result was less than 25 min.

In this paper, the previously proposed shaft-loaded blister test technique for the synchronous characterization of the surface and interface mechanical properties of a thin-film/substrate system is further studied theoretically. The large deflection problem of the steady shaft-loaded blistering thin film is reformulated by surrendering the small-rotation-angle assumption of the membrane, which was previously adopted in the out-of-plane and in-plane equilibrium and radial geometric equations. A new and more accurate analytical solution to this large deflection problem is presented and is used to improve the previously presented synchronous characterization theory. The new analytical solution is numerically compared with the previous analytical solution to confirm the superiority of the new analytical solution over the previous analytical solution. An experiment is conducted to verify the beneficial effect of the improved synchronous characterization theory on improving the characterization accuracy. [ABSTRACT FROM AUTHOR]

A circular capacitive rainfall sensor can operate from non-touch mode to touch mode; that is, under the action of enough rainwater, its movable electrode plate can form a circular contact area with its fixed electrode plate. Therefore, the weight of rainwater is borne by only its movable electrode plate in non-touch mode operation but by both its movable and fixed electrode plates in touch mode operation, and the total capacitance of its touch mode operation is much larger than that of its non-touch mode operation. Essential to its numerical design and calibration is the ability to predict the deflection shape of its moveable electrode plate to determine its total capacitance. This requires the analytical solution to the fluid–structure interaction problem of its movable electrode plate under rainwater. In our previous work, only the analytical solution for the fluid–structure interaction problem before its movable electrode plate touches its fixed electrode plate was obtained, and how to numerically design and calibrate a circular non-touch mode capacitive rainfall sensor was illustrated. In this paper, the analytical solution for the fluid–structure interaction problem after its movable electrode plate touches its fixed electrode plate is obtained, and how to numerically design and calibrate a circular touch mode capacitive rainfall sensor is illustrated for the first time. The numerical results show that the total capacitance and rainwater volume when the circular capacitive rainfall sensor operates in touch mode is indeed much larger than that when the same circular capacitive rainfall sensor operates in non-touch mode, and that the average increase in the maximum membrane stress per unit rainwater volume when the circular capacitive rainfall sensor operates in touch mode can be about 20 times smaller than that when the same circular capacitive rainfall sensor operates in non-touch mode. This is where the circular touch mode capacitive rainfall sensor excels. [ABSTRACT FROM AUTHOR]

As an important analytical method, the perturbation‐variation method combines the advantages of perturbation method and variational method, and is widely used for the solution of nonlinear plate and shell problems. In this study, the perturbation‐variation method is used to solve the large deformation problem of a flexible hyperbolic thin shallow shells with different moduli in tension and compression (bimodular effect). First, we establish the governing equations expressed in terms of three displacement components, in which the bimodular effect of materials is considered in physical equations and the large deformation of shell is considered in geometrical equations. Next, we use the perturbation‐variation method to solve the governing equations established, obtaining the perturbation‐variation solution up to third‐order. During the application of the method, the displacements are expressed into perturbation formulas of all levels first, and the unknown constants and functions in the perturbation formulas are determined by the extreme value conditions of the functional established (variational principle), hence the perturbation‐variation method gets its name from this practice. The numerical results from FEM basically agree with the perturbation‐variation solution obtained. The method proposed may be extended into the solution of similar partial differential equations established in applied fields. [ABSTRACT FROM AUTHOR]

A circular non-touch mode capacitive pressure sensor can operate in both transverse and normal uniform loading modes, but the elastic behavior of its movable electrode plate is different under the two different loading modes, making its input–output analytical relationships between pressure and capacitance different. This suggests that when such a sensor operates, respectively, in transverse and normal uniform loading modes, the theory of its numerical design and calibration is different, in other words, the theory for the transverse uniform loading mode (available in the literature) cannot be used as the theory for the normal uniform loading mode (not yet available in the literature). In this paper, a circular non-touch mode capacitive pressure sensor operating in normal uniform loading mode is considered. The elastic behavior of the movable electrode plate of the sensor under normal uniform loading is analytically solved with the improved governing equations, and the improved analytical solution obtained can be used to mathematically describe the movable electrode plate with larger elastic deflections, in comparison with the existing two analytical solutions in the literature. This provides a larger technical space for developing the circular non-touch mode capacitive pressure sensors used for measuring the static gas pressure (belonging to normal uniform loading). [ABSTRACT FROM AUTHOR]

In this study, the variational method and numerical simulation technique were used to solve the problem of bimodular functionally graded thin plates under large deformation. During the application of the variational method, the functional was established on the elastic strain energy of the plate while the variation in the functional was realized by changing undetermined coefficients in the functional. As a result, the classical Ritz method was adopted to obtain the important relationship between load and maximum deflection that is of great concern in engineering design. At the same time, the numerical simulation technique was also utilized by applying the software ABAQUS6.14.4, in which the bimodular effect and functionally graded properties of the materials were simulated by subareas in tension and compression, as well as the layering along the direction of plate thickness, respectively. This study indicates that the numerical simulation results agree with those from the variational solution, by comparing the maximum deflection of the plate, which verifies the validity of the variational solution obtained. The results presented in this study are helpful for the refined analysis and optimization design of flexible structures, which are composed of bimodular functionally graded materials, while the structure is under large deformation. [ABSTRACT FROM AUTHOR]

This study is concerned with the free vibration analysis of semi-closed shells of revolution with variable curvature, with a focus on the analytical solution to the natural frequency of such shells. A semi-closed shell of revolution with variable curvature is firstly divided into some narrow small shell segments, then the first small shell segment with a closed shell apex is approximated with a semi-closed conical shell, while the remaining small shell segments are approximated with a series of progressively larger truncated conical shells. Since each small conical shell is part of the large semi-closed shell of revolution with variable curvature and has to vibrate in sync with the large shell, the analytical solution to the axisymmetric free vibration of the large shell can be transformed into the analytical solutions to the synchronous free vibration of these small conical shells. The general solutions for the natural frequencies of all small conical shells are analytically derived under the condition that conical shells have zero meridional curvature. The undetermined constants in the general solutions are determined by letting all natural frequencies of these small conical shells be equal. Finally, the analytical solution for the axisymmetric free vibration of semi-closed shells of revolution with variable curvature is presented and is proved to be basically reliable by conducting a confirmatory experiment based on polymer 3D-printing technique. [ABSTRACT FROM AUTHOR]