Search

Your search keyword '"He, Kaize"' showing total 40 results
Start Over Author "He, Kaize"
40 results on '"He, Kaize"'

7. Proteomics in Duckweeds

Author

Fang, Yang, Du, Anping, Tan, Li, He, Kaize, Jin, Yanling, Tian, Xueping, Xu, Yaliang, Zhao, Hai, Kole, Chittaranjan, Series Editor, Cao, Xuan Hieu, editor, Fourounjian, Paul, editor, and Wang, Wenqin, editor

Published
2020
Full Text
View/download PDF

8. The Transcriptome in Landoltia punctata

Author

Fang, Yang, Du, Anping, Tan, Li, He, Kaize, Jin, Yanling, Ding, Yanqiang, Guo, Lin, Zhao, Hai, Kole, Chittaranjan, Series Editor, Cao, Xuan Hieu, editor, Fourounjian, Paul, editor, and Wang, Wenqin, editor

Published
2020
Full Text
View/download PDF

12. The Transcriptome in Landoltia punctata

Author

Fang, Yang, primary, Du, Anping, additional, Tan, Li, additional, He, Kaize, additional, Jin, Yanling, additional, Ding, Yanqiang, additional, Guo, Lin, additional, and Zhao, Hai, additional

Published
2020
Full Text
View/download PDF

13. Proteomics in Duckweeds

Author

Fang, Yang, primary, Du, Anping, additional, Tan, Li, additional, He, Kaize, additional, Jin, Yanling, additional, Tian, Xueping, additional, Xu, Yaliang, additional, and Zhao, Hai, additional

Published
2020
Full Text
View/download PDF

17. Additional file 1 of Insight into broad substrate specificity and synergistic contribution of a fungal α-glucosidase in Chinese Nong-flavor daqu

Author

Yi, Zhuolin, Chen, Lanchai, Jin, Yanling, Shen, Yi, Liu, Nian, Fang, Yang, Xiao, Yao, Wang, Xi, Peng, Kui, He, Kaize, and Zhao, Hai

Abstract

Supplementary Material 1: Table S1. Accession numbers of all those selected enzymes for Phylogenetic tree analysis in four subgroups of GH31 family. Table S2. The top 30 highest expression of genes related to carbohydrate-active enzymes at the highest temperature stage (N3) of NF daqu. Table S3. The relative high expression of alpha-glucosidase (GH31) genes at the high temperature stage (N3), and their relative expressions at the beginning stage (N1), the increasing temperature stage (N2) and mature stage (N4) of NF daqu. Table S4. Effect of metal ions and chemicals on the activity of NFAg31A. Table S5. The synergistic hydrolysis of soluble starch by different molar ratios and combinations of NFAg31A and two alpha-amylases (NFAmy13A and NFAmy13B). Table S6. The end products of synergistically hydrolyzing soluble starch by NFAg31A and two alpha-amylases (NFAmy13A and NFAmy13B) at molar ratio of 3:7:10. Figure S1. Schematic representation and purification of NFAg31A. Figure S2. Sequence alignment among NFAg31A and its homologous ?-glucosidases. Figure S3. Thermostability of NFEg31A at 39 ℃, 45 ℃ and 50 ℃

Published
2023
Full Text
View/download PDF

19. Insight into broad substrate specificity and synergistic contribution of a fungal α-glucosidase in Chinese Nong-flavor daqu.

Author

Yi, Zhuolin, Chen, Lanchai, Jin, Yanling, Shen, Yi, Liu, Nian, Fang, Yang, Xiao, Yao, Wang, Xi, Peng, Kui, He, Kaize, and Zhao, Hai

Subjects
GLUCOSIDASES, SOLID-state fermentation, BIOMACROMOLECULES, BIOLOGICAL evolution, MALTOSE, STARCH, CORNSTARCH, METAL ions
Abstract

Background: Chinese Nong-favor daqu, the presentative liquor starter of Baijiu, has been enriched with huge amounts of enzymes in degrading various biological macromolecules by openly man-made process for thousand years. According to previous metatranscriptomics analysis, plenty of α-glucosidases were identified to be active in NF daqu and played the key role in degrading starch under solid-state fermentation. However, none of α-glucosidases was characterized from NF daqu, and their actual functions in NF daqu were still unknown. Results: An α-glucosidase (NFAg31A, GH31-1 subfamily), the second highest expressed α-glucosidases in starch degradation of NF daqu, was directly obtained by heterologous expression in Escherichia coli BL21 (DE3). NFAg31A exhibited the highest sequence identities of 65.8% with α-glucosidase II from Chaetomium thermophilum, indicating its origin of fungal species, and it showed some similar features with homologous α-glucosidase IIs, i.e., optimal activity at pH ~ 7.0 and litter higher temperature of 45 ℃, well stability at 41.3 ℃ and a broad pH range of pH 6.0 to pH 10.0, and preference on hydrolyzing Glc-α1,3-Glc. Besides this preference, NFAg31A showed comparable activities on Glc-α1,2-Glc and Glc-α1,4-Glc, and low activity on Glc-α1,6-Glc, indicating its broad specificities on α-glycosidic substrates. Additionally, its activity was not stimulated by any of those detected metal ions and chemicals, and could be largely inhibited by glucose under solid-state fermentation. Most importantly, it exhibited competent and synergistic effects with two characterized α-amylases of NF daqu on hydrolyzing starch, i.e., all of them could efficiently degrade starch and malto-saccharides, two α-amylases showed advantage in degrading starch and long-chain malto-saccharides, and NFAg31A played the competent role with α-amylases in degrading short-chain malto-saccharides and the irreplaceable contribution in hydrolyzing maltose into glucose, thus alleviating the product inhibitions of α-amylases. Conclusions: This study provides not only a suitable α-glucosidase in strengthening the quality of daqu, but also an efficient way to reveal roles of the complicated enzyme system in traditional solid-state fermentation. This study would further stimulate more enzyme mining from NF daqu, and promote their actual applications in solid-state fermentation of NF liquor brewing, as well as in other solid-state fermentation of starchy industry in the future. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

28. Unraveling the Contribution of High Temperature Stage to Jiang-Flavor Daqu, a Liquor Starter for Production of Chinese Jiang-Flavor Baijiu, With Special Reference to Metatranscriptomics

Author

Yi, Zhuolin, primary, Jin, Yanling, additional, Xiao, Yao, additional, Chen, Lanchai, additional, Tan, Li, additional, Du, Anping, additional, He, Kaize, additional, Liu, Dayu, additional, Luo, Huibo, additional, Fang, Yang, additional, and Zhao, Hai, additional

Published
2019
Full Text
View/download PDF

30. Duckweed systems for eutrophic water purification through converting wastewater nutrients to high-starch biomass: comparative evaluation of three different genera (Spirodela polyrhiza, Lemna minor and Landoltia punctata) in monoculture or polyculture

Author

Chen, Guoke, primary, Fang, Yang, additional, Huang, Jun, additional, Zhao, Yonggui, additional, Li, Qi, additional, Lai, Fan, additional, Xu, Yaliang, additional, Tian, Xueping, additional, He, Kaize, additional, Jin, Yanling, additional, Tan, Li, additional, and Zhao, Hai, additional

Published
2018
Full Text
View/download PDF

36. Uniconazole-induced starch accumulation in the bioenergy crop duckweed (Landoltia punctata) II: transcriptome alterations of pathways involved in carbohydrate metabolism and endogenous hormone crosstalk

Author

Liu, Yang, primary, Fang, Yang, additional, Huang, Mengjun, additional, Jin, Yanling, additional, Sun, Jiaolong, additional, Tao, Xiang, additional, Zhang, Guohua, additional, He, Kaize, additional, Zhao, Yun, additional, and Zhao, Hai, additional

Published
2015
Full Text
View/download PDF

37. Uniconazole-induced starch accumulation in the bioenergy crop duckweed (Landoltia punctata) I: transcriptome analysis of the effects of uniconazole on chlorophyll and endogenous hormone biosynthesis

Author

Liu, Yang, primary, Fang, Yang, additional, Huang, Mengjun, additional, Jin, Yanling, additional, Sun, Jiaolong, additional, Tao, Xiang, additional, Zhang, Guohua, additional, He, Kaize, additional, Zhao, Yun, additional, and Zhao, Hai, additional

Published
2015
Full Text
View/download PDF

40. Structural identification and UPLC-ESI-QTOF-MS2 analysis of flavonoids in the aquatic plant Landoltia punctata and their in vitro and in vivo antioxidant activities.

Author

Tsolmon, Bolor, Fang, Yang, Yang, Tao, Guo, Ling, He, Kaize, Li, Guo-You, and Zhao, Hai

Subjects
*ANALYTICAL chemistry, *REACTIVE oxygen species, *CAENORHABDITIS elegans, *APIGENIN, *FLAVONOIDS, *ANTIOXIDANTS
Abstract

• Apigenin 6- C- [ β- D-apiofuranosyl-(1 → 2)]- β -D-glucopyranoside was isolated from L. punctata. • In total, 24 flavone glycosides were identified in L. punctata by UPLC-ESI-QTOF-MS2. • Apigenin 6- C- glycoside exhibited significant in vivo antioxidant activity in C. elegans. Duckweeds have long been consumed as vegetables in several South Asian countries. In this study of the chemical constituents of duckweed Landoltia punctata , a new compound, apigenin 6- C- [ β- D-apiofuranosyl-(1 → 2)]- β -D-glucopyranoside (1), and a previously LC–MS identified compound, quercetin 3- O - β -D-apiofuranoside (3), as well as three known compounds, luteolin 6- C -[ β -D-apiofuranosyl-(1 → 2)]- β -D-glucopyranoside (2), apigenin 6- C-β- D-glucopyranoside (4), and luteolin 7- O -neohespirodise (5), were isolated and identified on the basis of MS and NMR spectroscopic analyses and chemical derivations. In total, 24 flavonoids were identified in L. punctata 0001 by UPLC-ESI-QTOF-MS2. In DPPH and ABTS assays, 3 exhibited significant antioxidant activity with IC 50 values of 4.03 ± 1.31 µg/mL and 14.9 ± 2.28 µg/mL, respectively. In in vivo antioxidant activity assays, 1 significantly increased the survival rate of juglone-exposed Caenorhabditis elegans by 2 to 3-fold, and by 75% following thermal damage. Compounds 1 – 5 exhibited moderate scavenging capacities of intracellular reactive oxygen species in C. elegans exposed to H 2 O 2. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results