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Objective To establish a three-dimensional coordinate system that can accurately measure the recurrence rate after orthognathic surgery, and evaluate and analyze the degree of recurrence. Methods Data from patients who underwent orthognathic surgery in a hospital were selected to reconstruct three-dimensional images with spiral CT. The two researchers used the multiplane assisted positioning method to fix the points three times and screened them using intra-andintreclass correlation coefficients (ICC). Reproducible and accurate landmark points were drawn to establish different coordinate systems and calculate the facial asymmetry index (AI) to determine the coordinate system with the best mid-sagittal plane symmetry. This coordinate system and lateral radiographs were used to separately measure the recurrence rate, and evaluate and analyze the three-dimensional recurrence degree of orthognathic surgery. Results Ten landmark points that may be repeatedly fixed were obtained, including N (nasion), K (K point), ANS(anterior nasal spine), PNS (posterior nasal spine), Ptm(pterygomaxillary fissure), Gn (gnathion), IZ(IZ point), MZ (MZ point), Ms (mastoideale), Cor (coronion) and and Go (gonion). Three coordinate systems were established, and the most suitable coordinate system was the upper edge point of the left infraorbital foramen. The inner upper edge of the right infraorbital foramen and both sides of the midpoint of the ear points constituted the horizontal plane (HP), which passed through the outermost point of the left zygomatic frontal suture and the outermost point of the right zygomatic frontal suture and was perpendicular to the horizontal plane to constitute the coronal plane (CP). It was perpendicular to the two planes to form a sagittal plane (SP). Two-dimensional and three-dimensional measurements of recurrence were performed on 112 patients, and new three-dimensional recurrence evaluation results were obtained. Less than 40% had low recurrence, 40% to 61% had moderate recurrence, and greater than 61% had high recurrence. Conclusion This study established a three-dimensional coordinate system suitable for measuring the recurrence rate after orthognathic surgery, obtained a new three-dimensional recurrence evaluation result, and provided a clinical experimental basis for evaluating the effect of orthognathic surgery and improving stability.

Increasing evidence has shown that homologous recombination (HR) and metabolic reprogramming are essential for cellular homeostasis. These two processes are independent as well as closely intertwined. Nevertheless, they have rarely been reported in lung adenocarcinoma (LUAD). We analysed the genomic, immune microenvironment and metabolic microenvironment features under different HR activity states. Using cell cycle, EDU and cell invasion assays, we determined the impacts of si‐SHFM1 on the LUAD cell cycle, proliferation and invasion. The levels of isocitrate dehydrogenase (IDH) and α‐ketoglutarate dehydrogenase (α‐KGDH) were determined by ELISA in the NC and si‐SHFM1 groups of A549 cells. Finally, cell samples were used to extract metabolites for HPIC‐MS/MS to analyse central carbon metabolism. We found that high HR activity was associated with a poor prognosis in LUAD, and HR was an independent prognostic factor for TCGA‐LUAD patients. Moreover, LUAD samples with a high HR activity presented low immune infiltration levels, a high degree of genomic instability, a good response status to immune checkpoint blockade therapy and a high degree of drug sensitivity. The si‐SHFM1 group presented a significantly higher proportion of cells in the G0/G1 phase, lower levels of DNA replication, and significantly lower levels of cell migration and both TCA enzymes. Our current results indicated that there is a strong correlation between HR and the TCA cycle in LUAD. The TCA cycle can promote SHFM1‐mediated HR in LUAD, raising their activities, which can finally result in a poor prognosis and impair immunotherapeutic efficacy. [ABSTRACT FROM AUTHOR]

In this study, a three-dimensional electrochemical oxidation system was constructed to treat ammonia nitrogen wastewater generated from the tail gas absorption of a methionine producer by using a homemade MAC mixed with a GAC at a mass ratio of 1:2 as the particle electrode, with a RuO2-IrO2/Ti polar plate as the anode and a stainless steel plate as the cathode. The effects of current density, initial pH value of wastewater, plate spacing, NaCl concentration and particle filling amount on CODCr and NH4+-N removal were investigated through single-factor experiments, and the removal pathways of CODCr and NH4+-N under the system were initially explored via cyclic voltammetry curves, scanning electron microscopy and tertiary butanol quenching experiments. The experimental results showed that the average removal rate of CODCr was 91.03% and that of NH4+-N was 98.89% after electrolysis for 5 h under the conditions of a current density of 40 mA/cm2, no pH adjustment, the spacing of the electrode plates of 8 cm, the NaCl dosing concentration of 1 g/L, and the particle filling amount of 400 g/L. Under this experimental condition, the removal of CODCr occurred mainly through the indirect oxidation of active chlorine and ·OH, and the removal of NH4+-N mainly through the indirect oxidation of active chlorine. [ABSTRACT FROM AUTHOR]

Author(s): Isao Matsuura [1, 2, 3]; Natalia G. Denissova [1, 2, 3, 4]; Guannan Wang [1, 2, 3, 4]; Dongming He [1, 2, 3]; Jianyin Long [1, 2, 3]; Fang [...]

SMAD proteins can mediate transforming growth factor [Beta] (TGF-[Beta])-inducible transcriptional responses. Whereas SMAD can recognize specific DNA sequences, it is usually recruited to a promoter through interaction with a DNA-binding partner. In an effort to search for TGF-[Beta]-inducible genes, we used a subtractive screening method and identified human Smad7, which can antagonize TGF-[Beta] signaling and is rapidly up-regulated by TGF-[Beta]. In this report, we show that TGF-[Beta] can stabilize Smad7 mRNA and activate Smad7 transcription. The Smad7 promoter is the first TGF-[Beta] responsive promoter identified in vertebrates that contains the 8-bp palindromic SMAD-binding element (SBE), an optimal binding site previously identified by a PCR-based selection from random oligonucleotides by using recombinant Smad3 and Smad4. We demonstrate that on TGF-[Beta] treatment, endogenous SMAD complex can bind to a Smad7 promoter DNA as short as 14 or 16 bp that contains the 8-bp SBE in gel mobility shift and supershift assays. Our studies provide strong evidence that SMAD proteins can bind to a natural TGF-[Beta] responsive promoter independent of other sequence-specific transcription factors. We further show that, whereas recombinant Smad3 binds to the SBE, endogenous or even transfected Smad3 cannot bind to the SBE in the absence of Smad4. These findings have important implications in the identification of target genes of the TGF-[Beta]/SMAD signaling pathways.

The aim of this study was to evaluate the effect of SEMA3A released from matrigel on implant fixation in ovariectomized (OVX) rats. Sixty female rats were subjected to bilateral ovariectomy. Twelve weeks later, rats were randomly divided into three groups according to implants they accepted: (1) Control, implants with distilled water; (2) Matrigel, implants with matrigel coating; (3) Matrigel + SEMA3A, implants with coating of SEMA3A suspended in matrigel. Implants were inserted in metaphysis of proximal tibiae in all animas bilaterally. In vitro release of SEMA3A was tested using enzyme linked immunosorbent assay. In vitro release of SEMA3A was detectable during the first 10 days, and a burst release of was observed during the first 3 days. No significant difference was observed between Control and Matrigel group. The protective effects of SEMA3A in matrigel on peri-implant bone, implant osseointegration and fixation was confirmed. Compared to matrigel alone, SEMA3A suspended in matrigel increased percent bone volume by 88.7% and 83.3% ( p < 0.01), bone-to-implant contact ratio by 148.9% ( p < 0.01), and 24.8% ( p < 0.05), the maximal push-out force by 149.3% and 209.2% ( p < 0.01) at 4 and 8 weeks after implant insertion, respectively. Surface modification with SEMA3A suspended in matrigel improved implant osseointegration and fixation in the proximal tibiae of OVX rats. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2060-2065, 2017. [ABSTRACT FROM AUTHOR]