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1. Histopathology and SARS-CoV-2 Cellular Localization in Eye Tissues of COVID-19 Autopsies

Author

Stein, Sydney R., Ramelli, Sabrina C., Ramos-Benitez, Marcos J., Platt, Andrew P., Dickey, James M., Curran, Shelly J., Babyak, Ashley L., Valencia, Luis Perez, Richert, Mary E., Vannella, Kevin M., Chertow, Daniel S., Kleiner, David E., Hewitt, Stephen M., Young, Willie J., Young, Sarah P., Gasmi, Billel, De Melo, Michelly Sampaio, Desar, Sabina, Tadros, Saber, Nasir, Nadia, Jin, Xueting, Rajan, Sharika, Dikoglu, Esra, Ozkaya, Neval, Ylaya, Kris, Chung, Joon-Yong, Pittaluga, Stefania, Smith, Grace, Emanuel, Elizabeth R., Kelsall, Brian, Olivera, Justin A., Blawas, Megan, Grazioli, Alison, Hays, Nicole, Purcell, Madeleine, Singireddy, Shreya, Wu, Jocelyn, Raja, Katherine, Curto, Ryan, Chung, Jean, Borth, Amy, Bowers, Kimberly, Weichold, Anne, Minor, Paula, Moshref, Mirahmad, Kelly, Emily, Sajadi, Mohammad M., Scalea, Thomas M., Tran, Douglas, Madathil, Ronson J., Dahi, Siamak, Deatrick, Kristopher B., Krause, Eric M., Rabin, Joseph, Herrold, Joseph A., Tabatabai, Ali, Hochberg, Eric, Cornachione, Christopher, Levine, Andrea R., Saharia, Kapil K., Richards, Justin E., Elder, John, Burke, Allen, Mazzeffi, Michael A., Christenson, Robert, Chancer, Zackary, Abdulmahdi, Mustafa, Sopha, Sabrina, Goldberg, Tyler, Soherwardi, Shahabuddin, Sangwan, Yashvir, McCurdy, Michael T., Sudano, Kristen, Blume, Diane, Radin, Bethany, Arnouk, Madhat, Eagan, James W., Jr., Herr, Daniel L., Zerbe, Christa, Sen, H. Nida, Wang, Yujuan, Kodati, Shilpa, Lee, Jung Wha, Perez, Paola, Singh, Manmeet, Yinda, Kwe Claude, Platt, Andrew, Munster, Vincent J., de Wit, Emmie, Warner, Blake M., and Chan, Chi-Chao

Published
2023
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3. 433. Thromboinflammation in COVID-19 and association with death in patients on ECMO

Author

Platt, Andrew, primary, Callier, Viviane, additional, Hu, Zonghui, additional, Warner, Seth, additional, Stein, Syndey R, additional, Anders, Megan, additional, Ricotta, Emily, additional, Stantliff, Trevor M, additional, Wu, Jocelyn, additional, Hays, Nicole, additional, Raja, Katherine, additional, Curto, Ryan, additional, Purcell, Madeleine, additional, Ramelli, Sabrina, additional, Ramos-Benitez, Marcos J, additional, Lach, Izabella, additional, Dickey, James, additional, Strich, Jeffrey R, additional, Vannella, Kevin M, additional, Gibbons, Miranda, additional, Tabatabai, Ali, additional, Saharia, Kapil K, additional, Madathil, Ronson, additional, Rabin, Joseph, additional, Grazioli, Alison, additional, Follmann, Dean, additional, and Chertow, Daniel S, additional

Published
2023
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4. Histopathology and SARS-CoV-2 cellular localization in eye tissues of COVID-19 autopsies

Author

Sen, H. Nida, primary, Vannella, Kevin M., additional, Wang, Yujuan, additional, Chung, Joon-Yong, additional, Kodati, Shilpa, additional, Ramelli, Sabrina C., additional, Lee, Jung Wha, additional, Perez, Paola, additional, Stein, Sydney R., additional, Grazioli, Alison, additional, Dickey, James M., additional, Ylaya, Kris, additional, Singh, Manmeet, additional, Yinda, Kwe Claude, additional, Platt, Andrew, additional, Ramos-Benitez, Marcos J., additional, Zerbe, Christa, additional, Munster, Vincent J., additional, de Wit, Emmie, additional, Warner, Blake M, additional, Herr, Daniel L., additional, Rabin, Joseph, additional, Saharia, Kapil K, additional, Kleiner, David E., additional, Hewitt, Stephen M., additional, Chan, Chi-Chao, additional, Chertow, Daniel S., additional, Platt, Andrew P., additional, Curran, Shelly J., additional, Babyak, Ashley L., additional, Valencia, Luis Perez, additional, Richert, Mary E., additional, Young, Willie J., additional, Young, Sarah P., additional, Gasmi, Billel, additional, De Melo, Michelly Sampaio, additional, Desar, Sabina, additional, Tadros, Saber, additional, Nasir, Nadia, additional, Jin, Xueting, additional, Rajan, Sharika, additional, Dikoglu, Esra, additional, Ozkaya, Neval, additional, Pittaluga, Stefania, additional, Smith, Grace, additional, Emanuel, Elizabeth R., additional, Kelsall, Brian, additional, Olivera, Justin A., additional, Blawas, Megan, additional, Hays, Nicole, additional, Purcell, Madeleine, additional, Singireddy, Shreya, additional, Wu, Jocelyn, additional, Raja, Katherine, additional, Curto, Ryan, additional, Chung, Jean, additional, Borth, Amy, additional, Bowers, Kimberly, additional, Weichold, Anne, additional, Minor, Paula, additional, Moshref, Mirahmad, additional, Kelly, Emily, additional, Sajadi, Mohammad M., additional, Scalea, Thomas M., additional, Tran, Douglas, additional, Madathil, Ronson J., additional, Dahi, Siamak, additional, Deatrick, Kristopher B., additional, Krause, Eric M., additional, Herrold, Joseph A., additional, Tabatabai, Ali, additional, Hochberg, Eric, additional, Cornachione, Christopher, additional, Levine, Andrea R., additional, Saharia, Kapil K., additional, Richards, Justin E., additional, Elder, John, additional, Burke, Allen, additional, Mazzeffi, Michael A., additional, Christenson, Robert, additional, Chancer, Zackary, additional, Abdulmahdi, Mustafa, additional, Sopha, Sabrina, additional, Goldberg, Tyler, additional, Soherwardi, Shahabuddin, additional, Sangwan, Yashvir, additional, McCurdy, Michael T., additional, Sudano, Kristen, additional, Blume, Diane, additional, Radin, Bethany, additional, Arnouk, Madhat, additional, and Eagan, James W., additional

Published
2023
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5. Pulmonary Co-Infections Detected Premortem Underestimate Postmortem Findings in a COVID-19 Autopsy Case Series.

Author

Platt, Andrew P., Bradley, Benjamin T., Nasir, Nadia, Stein, Sydney R., Ramelli, Sabrina C., Ramos-Benitez, Marcos J., Dickey, James M., Purcell, Madeleine, Singireddy, Shreya, Hays, Nicole, Wu, Jocelyn, Raja, Katherine, Curto, Ryan, Salipante, Stephen J., Chisholm, Claire, Carnes, Stephanie, Marshall, Desiree A., Cookson, Brad T., Vannella, Kevin M., and Madathil, Ronson J.

Subjects
COVID-19 pandemic, AUTOPSY, COVID-19, MYCOSES, INTENSIVE care units, POLYMERASE chain reaction
Abstract

Bacterial and fungal co-infections are reported complications of coronavirus disease 2019 (COVID-19) in critically ill patients but may go unrecognized premortem due to diagnostic limitations. We compared the premortem with the postmortem detection of pulmonary co-infections in 55 fatal COVID-19 cases from March 2020 to March 2021. The concordance in the premortem versus the postmortem diagnoses and the pathogen identification were evaluated. Premortem pulmonary co-infections were extracted from medical charts while applying standard diagnostic definitions. Postmortem co-infection was defined by compatible lung histopathology with or without the detection of an organism in tissue by bacterial or fungal staining, or polymerase chain reaction (PCR) with broad-range bacterial and fungal primers. Pulmonary co-infection was detected premortem in significantly fewer cases (15/55, 27%) than were detected postmortem (36/55, 65%; p < 0.0001). Among cases in which co-infection was detected postmortem by histopathology, an organism was identified in 27/36 (75%) of cases. Pseudomonas, Enterobacterales, and Staphylococcus aureus were the most frequently identified bacteria both premortem and postmortem. Invasive pulmonary fungal infection was detected in five cases postmortem, but in no cases premortem. According to the univariate analyses, the patients with undiagnosed pulmonary co-infection had significantly shorter hospital (p = 0.0012) and intensive care unit (p = 0.0006) stays and significantly fewer extra-pulmonary infections (p = 0.0021). Bacterial and fungal pulmonary co-infection are under-recognized complications in critically ill patients with COVID-19. [ABSTRACT FROM AUTHOR]

Published
2023
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7. A Retrospective Study of Infection in Patients Requiring Extracorporeal Membrane Oxygenation Support

Author

Quintana, Megan T., primary, Mazzeffi, Michael, additional, Galvagno, Samuel M., additional, Herrera, Danny, additional, Boyajian, Gregory P., additional, Hays, Nicole M., additional, Kaczorowski, David, additional, Madathil, Ronson J., additional, Rector, Raymond P., additional, Tabatabai, Ali, additional, Herr, Daniel, additional, and Rabin, Joseph, additional

Published
2021
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8. Ultrasound‐assisted catheter directed thrombolysis for pulmonary embolus during extracorporeal membrane oxygenation

Author

Tran, Douglas, primary, Hays, Nicole, additional, Shah, Aakash, additional, Pasrija, Chetan, additional, Cires‐Drouet, Rafael S., additional, Toursavadkohi, Shahab A., additional, Mazzeffi, Michael A., additional, Herr, Daniel L., additional, Madathil, Ronson J., additional, Gammie, James S., additional, Griffith, Bartley P., additional, Deatrick, Kristopher B., additional, and Kaczorowski, David J., additional

Published
2021
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11. A porous collagen‐GAG scaffold promotes muscle regeneration following volumetric muscle loss injury.

Author

Panayi, Adriana C., Smit, Lucindi, Hays, Nicole, Udeh, Kodi, Endo, Yori, Li, Bin, Sakthivel, Dharaniya, Tamayol, Ali, Neppl, Ronald L., Orgill, Dennis P., Nuutila, Kristo, and Sinha, Indranil

Subjects
SKELETAL muscle injuries, ANABOLIC steroids, ANIMAL experimentation, BIOCHEMISTRY, COLLAGEN, CONVALESCENCE, GENE expression, HYPERTROPHY, LIFE skills, PHENOMENOLOGY, MICE, MUSCLES, MUSCLE diseases, REGENERATION (Biology), WOUND healing, QUADRICEPS muscle, SARCOPENIA, SKELETAL muscle
Abstract

Volumetric muscle loss (VML) is a segmental loss of skeletal muscle which commonly heals with fibrosis, minimal muscle regeneration, and loss of muscle strength. Treatment options for these wounds which promote functional recovery are currently lacking. This study was designed to investigate whether the collagen‐GAG scaffold (CGS) promotes functional muscle recovery following VML. A total of 66 C57/Bl6 mice were used in a three‐stage experiment. First, 24 animals were split into three groups which underwent sham injury or unilateral quadriceps VML injury with or without CGS implantation. Two weeks post‐surgery, muscle was harvested for histological and gene expression analysis. In the second stage, 18 mice underwent bilateral quadriceps VML injury, followed by weekly functional testing using a treadmill. In the third stage, 24 mice underwent sham or bilateral quadriceps VML injury with or without CGS implantation, with tissue harvested six weeks post‐surgery for histological and gene expression analysis. VML mice treated with CGS demonstrated increased remnant fiber hypertrophy versus both the VML with no CGS and uninjured groups. Both VML groups showed greater muscle fiber hypertrophy than non‐injured muscle. This phenomenon was still evident in the longer‐term experiment. The gene array indicated that the CGS promoted upregulation of factors involved in promoting wound healing and regeneration. In terms of functional improvement, the VML mice treated with CGS ran at higher maximum speeds than VML without CGS. A CGS was shown to enhance muscle hypertrophy in response to VML injury with a resultant improvement in functional performance. A gene array highlighted increased gene expression of multiple growth factors following CGS implantation. This suggests that implantation of a CGS could be a promising treatment for VML wounds. [ABSTRACT FROM AUTHOR]

Published
2020
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14. Dynamic nuclear protein interactions investigated using fluorescence lifetime and fluorescence fluctuation spectroscopy

Author

Siegel, Amanda P., Hays, Nicole M., and Day, Richard N.

Abstract

The discovery and engineering of novel fluorescent proteins (FPs) from diverse organisms is yielding fluorophores with exceptional characteristics for live-cell imaging. In particular, the development of FPs for Förster resonance energy transfer (FRET) microscopy and fluorescence fluctuation spectroscopy (FFS) provide important tools for monitoring dynamic protein interactions inside living cells. Fluorescence lifetime imaging microscopy (FLIM) quantitatively maps changes in the spatial distribution of donor FP lifetimes that result from FRET with acceptor FPs. FFS probes dynamic protein associations through its capacity to monitor localized protein diffusion. Here, we use FRET-FLIM combined with FFS in living cells to investigate changes in protein mobility due to protein-protein interactions involving transcription factors and chromatin modifying proteins that function in anterior pituitary gene regulation. The heterochromatin protein 1 alpha (HP1) plays a key role in the establishment and maintenance of heterochromatin through its interactions with histone methyltransferases. Recent studies, however, also highlight the importance of HP1 as a positive regulator of active transcription in euchromatin. Intriguingly, we observed that the transcription factor CCAAT/enhancer-binding protein alpha (C/EBP) interacts with HP1 in regions of pericentromeric heterochromatin in mouse pituitary cells. These observations prompted us to investigate the relationship between HP1 dynamic interactions in pituitary specific gene regulation.

Published
2012
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15. Monitoring protein interactions in living cells with fluorescence lifetime imaging microscopy.

Author

Sun Y, Hays NM, Periasamy A, Davidson MW, and Day RN

Subjects
Animals, Calibration, Luminescent Proteins analysis, Mice, Protein Binding, Signal Transduction, CCAAT-Enhancer-Binding Protein-alpha analysis, Fluorescence Resonance Energy Transfer methods, Fluorescent Dyes chemistry, Microscopy, Fluorescence methods, Protein Interaction Mapping
Abstract

Fluorescence lifetime imaging microscopy (FLIM) is now routinely used for dynamic measurements of signaling events inside single living cells, such as monitoring changes in intracellular ions and detecting protein-protein interactions. Here, we describe the digital frequency domain FLIM data acquisition and analysis. We describe the methods necessary to calibrate the FLIM system and demonstrate how they are used to measure the quenched donor fluorescence lifetime that results from Förster Resonance Energy Transfer (FRET). We show how the "FRET-standard" fusion proteins are used to validate the FLIM system for FRET measurements. We then show how FLIM-FRET can be used to detect the dimerization of the basic leucine zipper (B Zip) domain of the transcription factor CCAAT/enhancer binding protein α in the nuclei of living mouse pituitary cells. Importantly, the factors required for the accurate determination and reproducibility of lifetime measurements are described in detail., (Copyright © 2012 Elsevier Inc. All rights reserved.)

Published
2012
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