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9 results on '"Hawar, Amangul"'

4. ScATG8 Gene Cloned from Desert Moss Syntrichia caninervis Exhibits Multiple Stress Tolerance.

Author

Cao, Ting, Haxim, Yakupjan, Liu, Xiujin, Yang, Qilin, Hawar, Amangul, Waheed, Abdul, Li, Xiaoshuang, and Zhang, Daoyuan

Subjects
MOLECULAR cloning, CRUST vegetation, ABIOTIC stress, DESERTS, PLANT breeding
Abstract

Syntrichia caninervis is the dominant species of biological soil crust in the desert, including the Gurbantunggut Desert in China. It is widely distributed in drylands and considered to be a new model of vegetative desiccation tolerance moss. Here, we cloned an ATG8 gene from S. caninervis and confirmed its function under multiple abiotic stresses, both in situ and in Physcomitrium patens. The results showed that the ScATG8 gene encoded a protein with a highly conserved ATG8 functional domain. ScATG8 gene was increasingly expressed under different abiotic stresses. Under desiccation stress, the overexpression of ScATG8 enhanced the tolerance of S. caninervis and its ability to scavenge ROS. In addition, ScATG8 overexpression promoted the growth of P. patens under multiple stress conditions. Thus, ScATG8 may be a multifunctional gene, and it plays a critical role in the survival of S. caninervis under various abiotic stresses. Our results provide new insights into the function of ATG8 in enabling desiccation tolerance and open up more possibilities for subsequent plant molecular breeding and the mining of the resistance genes of S. caninervis and other moss species. [ABSTRACT FROM AUTHOR]

Published
2024
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5. Capture of regulatory factors via CRISPR–dCas9 for mechanistic analysis of fine-tuned SERRATEexpression in Arabidopsis

Author

Chen, Wei, Wang, Jingyi, Wang, Zijing, Zhu, Tao, Zheng, Yuchen, Hawar, Amangul, Chang, Yongsheng, Wang, Xin, Li, Dongbao, Wang, Guangling, Yang, Wen, Zhao, Yanjie, Chen, Dijun, Yuan, Yuren Adam, and Sun, Bo

Abstract

SERRATE (SE) plays an important role in many biological processes and under biotic stress resistance. However, little about the control of SEhas been clarified. Here we present a method named native chromatin-associated proteome affinity by CRISPR–dCas9 (CASPA–dCas9) to holistically capture native regulators of the SElocus. Several key regulatory factors including PHYTOCHROME RAPIDLY REGULATED 2 (PAR2), WRKY DNA-binding protein 19 (WRKY19) and the MYB-family protein MYB27 of SEare identified. MYB27 recruits the long non-coding RNA–PRC2 (SEAIR–PRC2) complex for H3K27me3 deposition on exon 1 of SEand subsequently represses SEexpression, while PAR2–MYB27 interaction inhibits both the binding of MYB27 on the SEpromoter and the recruitment of SEAIR–PRC2 by MYB27. The interaction between PAR2 and MYB27 fine-tunes the SEexpression level at different developmental stages. In addition, PAR2 and WRKY19 synergistically promote SEexpression for pathogen resistance. Collectively, our results demonstrate an efficient method to capture key regulators of target genes and uncover the precise regulatory mechanism for SE.

Published
2024
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8. SbNAC2 enhances abiotic stress tolerance by upregulating ROS scavenging activities and inducing stress-response genes in sorghum

Author

Jin, Xueying, primary, Long, Yufei, additional, Xiong, Shiqi, additional, Yang, Zhen, additional, Chen, Wei, additional, Hawar, Amangul, additional, Chi, Xiaoyu, additional, Chen, Yaxin, additional, Luo, Huilian, additional, Qi, Jinliang, additional, Lu, Guihua, additional, Dai, Lingyan, additional, Yang, Yonghua, additional, and Sun, Bo, additional

Published
2021
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