Search

Your search keyword '"Haughey, Norman J."' showing total 516 results
Start Over Author "Haughey, Norman J."
516 results on '"Haughey, Norman J."'

3. Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single‐particle analysis platforms

Author

Arab, Tanina, Mallick, Emily R, Huang, Yiyao, Dong, Liang, Liao, Zhaohao, Zhao, Zezhou, Gololobova, Olesia, Smith, Barbara, Haughey, Norman J, Pienta, Kenneth J, Slusher, Barbara S, Tarwater, Patrick M, Tosar, Juan Pablo, Zivkovic, Angela M, Vreeland, Wyatt N, Paulaitis, Michael E, and Witwer, Kenneth W

Subjects
Biochemistry and Cell Biology, Biological Sciences, Bioengineering, Nanotechnology, Biomarkers, Cell Line, Chromatography, Gel, Extracellular Vesicles, Humans, Microfluidics, Microscopy, Electron, Transmission, Nanoparticles, Particle Size, Polystyrenes, Single Molecule Imaging, Ultracentrifugation, Ultrafiltration, ectosomes, exosomes, extracellular vesicles, microvesicles, nanoflow cytometry, nanoparticle tracking analysis, resistive pulse sensing, single particle interferometric reflectance imaging sensing, Biochemistry and cell biology
Abstract

We compared four orthogonal technologies for sizing, counting, and phenotyping of extracellular vesicles (EVs) and synthetic particles. The platforms were: single-particle interferometric reflectance imaging sensing (SP-IRIS) with fluorescence, nanoparticle tracking analysis (NTA) with fluorescence, microfluidic resistive pulse sensing (MRPS), and nanoflow cytometry measurement (NFCM). EVs from the human T lymphocyte line H9 (high CD81, low CD63) and the promonocytic line U937 (low CD81, high CD63) were separated from culture conditioned medium (CCM) by differential ultracentrifugation (dUC) or a combination of ultrafiltration (UF) and size exclusion chromatography (SEC) and characterized by transmission electron microscopy (TEM) and Western blot (WB). Mixtures of synthetic particles (silica and polystyrene spheres) with known sizes and/or concentrations were also tested. MRPS and NFCM returned similar particle counts, while NTA detected counts approximately one order of magnitude lower for EVs, but not for synthetic particles. SP-IRIS events could not be used to estimate particle concentrations. For sizing, SP-IRIS, MRPS, and NFCM returned similar size profiles, with smaller sizes predominating (per power law distribution), but with sensitivity typically dropping off below diameters of 60 nm. NTA detected a population of particles with a mode diameter greater than 100 nm. Additionally, SP-IRIS, MRPS, and NFCM were able to identify at least three of four distinct size populations in a mixture of silica or polystyrene nanoparticles. Finally, for tetraspanin phenotyping, the SP-IRIS platform in fluorescence mode was able to detect at least two markers on the same particle, while NFCM detected either CD81 or CD63. Based on the results of this study, we can draw conclusions about existing single-particle analysis capabilities that may be useful for EV biomarker development and mechanistic studies.

Published
2021

4. Current Challenges and Solutions in Research and Clinical Care of Older Persons Living with HIV: Findings Presented at the 9th International Workshop on HIV and Aging

Author

Sundermann, Erin E, Erlandson, Kristine M, Pope, Caitlin N, Rubtsova, Anna, Montoya, Jessica, Moore, Alison A, Marzolini, Catia, O'Brien, Kelly K, Pahwa, Savita, Payne, Brendan AI, Rubin, Leah H, Walmsley, Sharon, Haughey, Norman J, Montano, Monty, Karris, Maile Y, Margolick, Joseph B, and Moore, David J

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Clinical Sciences, HIV/AIDS, Infectious Diseases, Sexually Transmitted Infections, Neurosciences, Aging, Infection, Good Health and Well Being, Aged, Aged, 80 and over, Comorbidity, Congresses as Topic, Disease Management, Female, HIV Infections, Humans, Inflammation, Life Expectancy, Male, Menopause, Research, HIV, aging, comorbidities, inflammation, polypharmacy, intervention, Virology, Clinical sciences
Abstract

In the era of effective antiretroviral therapy, the number of older people with HIV (PWH) is increasing, and those aging with HIV are experiencing an increasing burden of age-associated comorbidities. Life expectancy among older PWH is approaching that of demographically comparable HIV-uninfected (HIV-) adults. With this changing demographic of PWH come new challenges for researchers and clinicians in how to identify, address, and manage the complex interplay of treated HIV infection and aging-associated factors. In response to these challenges, the annual International Workshop on HIV and Aging was initiated in 2009 as a multidisciplinary platform for scientific discourse on the research and clinical complications arising from the aging population of PWH. The multidisciplinary nature of the workshop has resulted in a wide range of topics addressed over the past 9 years, from basic mechanisms in aging and HIV pathogenesis, to epidemiology of aging within large cohorts, interventions, and implementation of clinical programs. Herein, we summarize the key topics discussed at the 9th Annual International Workshop on HIV and Aging 2018, including "inflammaging," mitochondrial dysfunction, exercise interventions, HIV-associated neurocognitive impairment, metabolic dysfunction, menopause, and polypharmacy. In addition to recent developments in research and clinical care, we discuss open questions and future research directions required to better understand the interaction of HIV and aging.

Published
2019

7. Contributors

Author

Abdulai-Saiku, Samira, primary, Appel, Stanley H., additional, Arnold, Arthur P., additional, Arnold, Lisa M., additional, Arnold, Robert M., additional, Bacha, Alexa, additional, Backonja, Miroslav “Misha”, additional, Bailey, Zinzi D., additional, Bateman, Lucinda, additional, Beers, David R., additional, Berti, Anna, additional, Bhatnagar, Mamta, additional, Binder, Devin K., additional, Boido, Marina, additional, Boldrini, Maura, additional, Borsook, David, additional, Breakefield, Xandra O., additional, Brown, Robert H., additional, Burstein, Rami, additional, Butelman, Eduardo R., additional, Caplan, Louis R., additional, Chen, S., additional, Chesselet, Marie-Françoise, additional, Clemens, Stefan, additional, Clemens, Paula R., additional, Coyle, Joseph T., additional, DeWitt, John C., additional, Dubal, Dena B., additional, Dubljević, Veljko, additional, Feldman, Eva L., additional, Fischer, Beth A., additional, Flux, M.C., additional, Fortin, J.S., additional, Frasca, Angelisa, additional, Garbarini, Francesca, additional, Gasser, Thomas, additional, Gillespie, Charles F., additional, Gold, Michael S., additional, Gold, Stefan M., additional, Hagerman, Randi, additional, Hamel, Regan, additional, Haney, Craig, additional, Harris, James C., additional, Hassani, Sara, additional, Haughey, Norman J., additional, Heng, Vibol, additional, Horn, J., additional, Ionescu, Rosana-Bristena, additional, Iorio, Raffaele, additional, Irwin, David J., additional, Kaminski, Henry J., additional, Khammash, Dalia, additional, Khurana, Vikram, additional, Kilstrup-Nielsen, Charlotte, additional, Kim, Bhumsoo, additional, Kim, Boram, additional, Klein, Marieke, additional, Koen, Nastassja, additional, Konopaske, Glenn T., additional, Korecka, Joanna A., additional, Kornum, Birgitte Rahbek, additional, Kreek, Mary Jeanne, additional, Kristensson, Krister, additional, Krzak, Grzegorz, additional, Kusner, Linda L., additional, Landsberger, Nicoletta, additional, Lee, Edward B., additional, Li, Tong, additional, Liberski, Paweł P., additional, Lochner, Christine, additional, Lowry, Christopher A., additional, Mann, J. John, additional, Marincowitz, Clara, additional, Mayer, E.A., additional, Mayer, E.D., additional, McCall, Iris Coates, additional, McCullough, Louise D., additional, Meaney, Michael J., additional, Melo de Gusmao, Claudio, additional, Menesgere, Abhishek L., additional, Mignot, Emmanuel, additional, Mobley, William C., additional, Montalvo, Mayra, additional, Moreland-Capuia, Alisha R., additional, Neppi-Modona, Marco, additional, Nicaise, Alexandra M., additional, Nishi, Rae, additional, O'Toole, Orna, additional, Overk, Cassia, additional, Ozelius, Laurie, additional, Parsons, Matthew P., additional, Penticoff, H.B., additional, Peruzzotti-Jametti, Luca, additional, Peters, Owen M., additional, Peterson, Allison, additional, Phan, Jessica M., additional, Pittock, Sean J., additional, Pluchino, Stefano, additional, Polk, Thad A., additional, Puwanant, Araya, additional, Rajagopal, Shreya K., additional, Ravindranath, Vijayalakshmi, additional, Raymond, Lynn A., additional, Reed, Brian, additional, Ressler, Kerry J., additional, Ritchie, Diane L., additional, Rubin, Leah H., additional, Sakowski, Stacey A., additional, Saporta, Mario A., additional, Savonenko, Alena V., additional, Scharfman, Helen E., additional, Shapiro, Bruce K., additional, Sharma, Nutan, additional, Shaw, Cayce K., additional, Shy, Michael E., additional, Sikorska, Beata, additional, Silverman, Ethan J., additional, Simon, Roger P., additional, Simonyan, Kristina, additional, Sims-Robinson, Catrina, additional, Smeyne, Richard Jay, additional, Smith, Clay, additional, Smith, Colin, additional, Srinivasan, Sharan R., additional, Stein, Dan J., additional, Stephen, Christopher D., additional, Subramanian, Indu, additional, Szabo, Edina, additional, Thomas, Alissa A., additional, Tovar-y-Romo, Luis B., additional, Vahabzadeh, Arshya, additional, Vercelli, Alessandro, additional, Viera-Ortiz, Ashley, additional, Wallin, Mitchell T., additional, Werling, Donna M., additional, Wichmann, Thomas, additional, Wiley, Clayton A., additional, Williams, David R., additional, Willis, Cory, additional, Wong, Philip C., additional, Yuferov, Vadim, additional, Zhao, Weihua, additional, Zigmond, Michael J., additional, and Živković, Saša A., additional

Published
2023
Full Text
View/download PDF

8. Cognitive Trajectory Phenotypes in Human Immunodeficiency Virus-Infected Patients.

Author

Dastgheyb, Raha M, Sacktor, Ned, Franklin, Donald, Letendre, Scott, Marcotte, Thomas, Heaton, Robert, Grant, Igor, McArthur, Justin C, Rubin, Leah H, and Haughey, Norman J

Subjects
Biomedical and Clinical Sciences, Clinical Sciences, Mental Health, HIV/AIDS, Brain Disorders, Behavioral and Social Science, Neurosciences, Clinical Research, Mind and Body, Acquired Cognitive Impairment, Aetiology, 2.1 Biological and endogenous factors, Neurological, Mental health, Adult, Antirheumatic Agents, Cognition, Cognitive Dysfunction, Cohort Studies, Electronic Data Processing, Executive Function, Female, HIV Infections, Humans, Learning, Male, Memory, Short-Term, Mental Recall, Middle Aged, Neuropsychological Tests, Phenotype, Prevalence, Speech Disorders, Verbal Learning, HIV, AIDS, cognition, HAND, phenotypes, machine learning, Public Health and Health Services, Virology, Clinical sciences, Epidemiology, Public health
Abstract

ObjectiveThe presentation of cognitive impairments in HIV-infected individuals has transformed since the introduction of antiretroviral therapies. Although the overall prevalence of cognitive impairments has not changed considerably, frank dementia is now infrequent, and milder forms of cognitive impairments predominate. Mechanistic insights to the underlying causes of these residual cognitive impairments have been elusive, in part due to the heterogenous etiology of cognitive dysfunction in this population. Here, we sought to categorize longitudinal change in HIV-infected patients based on the performance in specific cognitive domains.DesignThis study consisted of 193 participants from the CHARTER cohort with detailed demographic, clinical, and neuropsychological testing data obtained from 2 study visits interspersed by ∼6 months. Cognitive testing assessed executive function, learning and delayed recall, working memory, verbal fluency, speed of information processing, and motor skills. Change scores were calculated for each domain between the 2 study visits. Dimension reduction and clustering was accomplished by principal component analysis of change scores and k-means clustering to identify cognitive domains that group together and groups of subjects with similar patterns of change.ResultsWe identified 4 distinct cognitive change phenotypes that included declines in: (1) verbal fluency, (2) executive function (3) learning and recall, and (4) motor function, with approximately equal numbers of participants in each phenotype.ConclusionsEach of the 4 cognitive change phenotypes identify deficits that imply perturbations in specific neural networks. Future studies will need to validate if cognitive change phenotypes are associated with alterations in associated neural pathways.

Published
2019

9. Impaired insulin sensitivity is associated with worsening cognition in HIV-infected patients.

Author

Khuder, Saja S, Chen, Suming, Letendre, Scott, Marcotte, Thomas, Grant, Igor, Franklin, Donald, Rubin, Leah H, Margolick, Joseph B, Jacobson, Lisa P, Sacktor, Ned, D'Souza, Gypsyamber, Stosor, Valentina, Lake, Jordan E, Rapocciolo, Giovanna, McArthur, Justin C, Dickens, Alex M, and Haughey, Norman J

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Clinical Research, Diabetes, HIV/AIDS, Aetiology, 2.1 Biological and endogenous factors, Adult, Anti-HIV Agents, C-Peptide, Case-Control Studies, Cognition, Cognitive Dysfunction, Cohort Studies, Female, HIV Infections, Humans, Hyperinsulinism, Insulin Resistance, Lipoproteins, Male, Middle Aged, Clinical Sciences, Neurosciences, Cognitive Sciences, Neurology & Neurosurgery, Clinical sciences
Abstract

ObjectiveTo determine the association of insulin sensitivity and metabolic status with declining cognition in HIV-infected individuals.MethodsWe conducted targeted clinical and metabolic measures in longitudinal plasma samples obtained from HIV-infected patients enrolled in the Central Nervous System HIV Anti-Retroviral Therapy Effects Research Study (CHARTER). Findings were validated with plasma samples from the Multicenter AIDS Cohort Study (MACS). Patients were grouped according to longitudinally and serially assessed cognitive performance as having stably normal or declining cognition.ResultsPatients with declining cognition exhibited baseline hyperinsulinemia and elevated plasma c-peptide levels with normal c-peptide/insulin ratios, suggesting that insulin production was increased, but insulin clearance was normal. The association of hyperinsulinemia with worsening cognition was further supported by low high-density lipoprotein (HDL), high low-density lipoprotein/HDL ratio, and elevated cholesterol/HDL ratio compared to patients with stably normal cognition.ConclusionsThese findings suggest that hyperinsulinemia and impaired insulin sensitivity are associated with cognitive decline in antiretroviral therapy-treated HIV-infected patients.

Published
2019

11. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Author

Celbiologie, Cell Biology, Metabolism & Cancer - Cellbiology, Welsh, Joshua A, Goberdhan, Deborah C I, O'Driscoll, Lorraine, Buzas, Edit I, Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A P, Erdbrügger, Uta, Falcon-Perez, Juan M, Fu, Qing-Ling, Hill, Andrew F, Lenassi, Metka, Lim, Sai Kiang, Mahoney, Mỹ G, Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C, Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M, Brucale, Marco, Burger, Dylan, Carney, Randy P, Cocucci, Emanuele, Colombo, Federico, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L, Haughey, Norman J, Hendrix, An, Ivanov, Alexander R, Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A, Ku'ulei-Lyn Faustino, Vroniqa, Kyburz, Diego, Lässer, Cecilia, Lennon, Kathleen M, Lötvall, Jan, Maddox, Adam L, Martens-Uzunova, Elena S, Mizenko, Rachel R, Newman, Lauren A, Ridolfi, Andrea, Rohde, Eva, Rojalin, Tatu, Rowland, Andrew, Saftics, Andras, Sandau, Ursula S, Saugstad, Julie A, Shekari, Faezeh, Swift, Simon, Ter-Ovanesyan, Dmitry, Tosar, Juan P, Useckaite, Zivile, Valle, Francesco, Varga, Zoltan, van der Pol, Edwin, van Herwijnen, Martijn J C, Wauben, Marca H M, Wehman, Ann M, Williams, Sarah, Zendrini, Andrea, Zimmerman, Alan J, Misev Consortium, Théry, Clotilde, Witwer, Kenneth W, Stoorvogel, Willem, Celbiologie, Cell Biology, Metabolism & Cancer - Cellbiology, Welsh, Joshua A, Goberdhan, Deborah C I, O'Driscoll, Lorraine, Buzas, Edit I, Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A P, Erdbrügger, Uta, Falcon-Perez, Juan M, Fu, Qing-Ling, Hill, Andrew F, Lenassi, Metka, Lim, Sai Kiang, Mahoney, Mỹ G, Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C, Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M, Brucale, Marco, Burger, Dylan, Carney, Randy P, Cocucci, Emanuele, Colombo, Federico, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L, Haughey, Norman J, Hendrix, An, Ivanov, Alexander R, Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A, Ku'ulei-Lyn Faustino, Vroniqa, Kyburz, Diego, Lässer, Cecilia, Lennon, Kathleen M, Lötvall, Jan, Maddox, Adam L, Martens-Uzunova, Elena S, Mizenko, Rachel R, Newman, Lauren A, Ridolfi, Andrea, Rohde, Eva, Rojalin, Tatu, Rowland, Andrew, Saftics, Andras, Sandau, Ursula S, Saugstad, Julie A, Shekari, Faezeh, Swift, Simon, Ter-Ovanesyan, Dmitry, Tosar, Juan P, Useckaite, Zivile, Valle, Francesco, Varga, Zoltan, van der Pol, Edwin, van Herwijnen, Martijn J C, Wauben, Marca H M, Wehman, Ann M, Williams, Sarah, Zendrini, Andrea, Zimmerman, Alan J, Misev Consortium, Théry, Clotilde, Witwer, Kenneth W, and Stoorvogel, Willem

Published
2024

12. Minimal information for studies of extracellular vesicles (MISEV2023) : from basic to advanced approaches

Author

Welsh, Joshua A., Goberdhan, Deborah C. I., O'Driscoll, Lorraine, Buzas, Edit I., Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A. P., Erdbrügger, Uta, Falcon-Perez, Juan M., Fu, Qing-Ling, Hill, Andrew F., Lenassi, Metka, Lim, Sai Kiang, Mahoney, Mỹ G., Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C., Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M., Brucale, Marco, Burger, Dylan, Carney, Randy P., Cocucci, Emanuele, Colombo, Federico, Crescitelli, Rossella, Eichenberger, Ramon, Hanser, Edveena, Harris, Adrian L., Haughey, Norman J., Hendrix, An, Ivanov, Alexander R., Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A., Ku'ulei-Lyn Faustino, Vroniqa, Kyburz, Diego, Lässer, Cecilia, Lennon, Kathleen M., Lötvall, Jan, Maddox, Adam L., Martens-Uzunova, Elena S., Mizenko, Rachel R., Newman, Lauren A., Ridolfi, Andrea, Rohde, Eva, Rojalin, Tatu, Rowland, Andrew, Saftics, Andras, Sandau, Ursula S., Saugstad, Julie A., Shekari, Faezeh, Swift, Simon, Ter-Ovanesyan, Dmitry, Tosar, Juan P., Useckaite, Zivile, Valle, Francesco, Varga, Zoltan, van der Pol, Edwin, van Herwijnen, Martijn J. C., Wauben, Marca H. M., Wehman, Ann M., Williams, Sarah, Zendrini, Andrea, Zimmerman, Alan J, Misev Consortium, Théry, Clotilde, Witwer, Kenneth W., Welsh, Joshua A., Goberdhan, Deborah C. I., O'Driscoll, Lorraine, Buzas, Edit I., Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A. P., Erdbrügger, Uta, Falcon-Perez, Juan M., Fu, Qing-Ling, Hill, Andrew F., Lenassi, Metka, Lim, Sai Kiang, Mahoney, Mỹ G., Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C., Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M., Brucale, Marco, Burger, Dylan, Carney, Randy P., Cocucci, Emanuele, Colombo, Federico, Crescitelli, Rossella, Eichenberger, Ramon, Hanser, Edveena, Harris, Adrian L., Haughey, Norman J., Hendrix, An, Ivanov, Alexander R., Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A., Ku'ulei-Lyn Faustino, Vroniqa, Kyburz, Diego, Lässer, Cecilia, Lennon, Kathleen M., Lötvall, Jan, Maddox, Adam L., Martens-Uzunova, Elena S., Mizenko, Rachel R., Newman, Lauren A., Ridolfi, Andrea, Rohde, Eva, Rojalin, Tatu, Rowland, Andrew, Saftics, Andras, Sandau, Ursula S., Saugstad, Julie A., Shekari, Faezeh, Swift, Simon, Ter-Ovanesyan, Dmitry, Tosar, Juan P., Useckaite, Zivile, Valle, Francesco, Varga, Zoltan, van der Pol, Edwin, van Herwijnen, Martijn J. C., Wauben, Marca H. M., Wehman, Ann M., Williams, Sarah, Zendrini, Andrea, Zimmerman, Alan J, Misev Consortium, Théry, Clotilde, and Witwer, Kenneth W.

Abstract

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

Published
2024

13. Minimal information for studies of extracellular vesicles (MISEV2023):From basic to advanced approaches

Author

Welsh, Joshua A., Goberdhan, Deborah C.I., O’Driscoll, Lorraine, Buzas, Edit I., Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A.P., Erdbrügger, Uta, Falcon-Perez, Juan M., Fu, Qing Ling, Hill, Andrew F., Lenassi, Metka, Lim, Sai Kiang, Mahoney, M. ỹ.G., Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C., Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M., Brucale, Marco, Burger, Dylan, Carney, Randy P., Cocucci, Emanuele, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L., Haughey, Norman J., Benediktsdóttir, Berglind E., Gargiulo, Ernesto, Gori, Alessandro, Guo, Wei, Hufnagel, Antonia, Di Ianni, Emilio, Jacobsen, Soren, Li, Bo, Mellergaard, Maiken, Nejsum, Peter, Nolan, John P., Østergaard, Ole, Yarani, Reza, Yin, Hang, Welsh, Joshua A., Goberdhan, Deborah C.I., O’Driscoll, Lorraine, Buzas, Edit I., Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A.P., Erdbrügger, Uta, Falcon-Perez, Juan M., Fu, Qing Ling, Hill, Andrew F., Lenassi, Metka, Lim, Sai Kiang, Mahoney, M. ỹ.G., Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C., Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M., Brucale, Marco, Burger, Dylan, Carney, Randy P., Cocucci, Emanuele, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L., Haughey, Norman J., Benediktsdóttir, Berglind E., Gargiulo, Ernesto, Gori, Alessandro, Guo, Wei, Hufnagel, Antonia, Di Ianni, Emilio, Jacobsen, Soren, Li, Bo, Mellergaard, Maiken, Nejsum, Peter, Nolan, John P., Østergaard, Ole, Yarani, Reza, and Yin, Hang

Abstract

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly., Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

Published
2024

20. Inhibiting tau-induced elevated nSMase2 activity and ceramides is therapeutic in murine Alzheimer’s disease

Author

Tallon, Carolyn, primary, Bell, Benjamin J, additional, Malvankar, Medhinee M, additional, Deme, Pragney, additional, Nogueras-Ortiz, Carlos, additional, Eren, Erden, additional, Thomas, Ajit G, additional, Hollinger, Kristen R, additional, Pal, Arindom, additional, Mustapic, Maja, additional, Huang, Meixiang, additional, Coleman, Kaleem, additional, Joe, Tawnjerae R, additional, Rais, Rana, additional, Haughey, Norman J, additional, Kapogiannis, Dimitrios, additional, and Slusher, Barbara S, additional

Published
2023
Full Text
View/download PDF

21. Neutral sphingomyelinase 2 is required for HIV-1 maturation

Author

Waheed, Abdul A., primary, Zhu, Yanan, additional, Agostino, Eva, additional, Naing, Lwar, additional, Hikichi, Yuta, additional, Soheilian, Ferri, additional, Yoo, Seung-Wan, additional, Song, Yun, additional, Zhang, Peijun, additional, Slusher, Barbara S., additional, Haughey, Norman J., additional, and Freed, Eric O., additional

Published
2023
Full Text
View/download PDF

22. Inhibition of neutral sphingomyelinase 2 impairs HIV-1 envelope formation and substantially delays or eliminates viral rebound

Author

Yoo, Seung-Wan, primary, Waheed, Abdul A., additional, Deme, Pragney, additional, Tohumeken, Sehmus, additional, Rais, Rana, additional, Smith, Matthew D., additional, DeMarino, Catherine, additional, Calabresi, Peter A., additional, Kashanchi, Fatah, additional, Freed, Eric O., additional, Slusher, Barbara S., additional, and Haughey, Norman J., additional

Published
2023
Full Text
View/download PDF

26. nSMase2 inhibition reduces tau propagation in Alzheimer’s Disease mouse models

Author

Tallon, Carolyn, primary, Bell, Benjamin J, additional, Malvankar, Medhinee, additional, Thomas, Ajit G, additional, Yoo, Seung Wan, additional, Pal, Arindom, additional, Wu, Ying, additional, Coleman, Kaleem, additional, Joe, Tawnjerae R, additional, Sharma, Anjali, additional, Eren, Erden, additional, Zhu, Xiaolei, additional, Kannan, Rangaramanujam M, additional, Kapogiannis, Dimitrios, additional, Haughey, Norman J, additional, Rais, Rana, additional, and Slusher, Barbara S, additional

Published
2022
Full Text
View/download PDF

28. Oxysterol misbalance critically contributes to Wilson disease pathogenesis

Author

Dev, Som, primary, Muchenditsi, Abigael, additional, Gottlieb, Aline, additional, Deme, Pragney, additional, Murphy, Sean, additional, Gabrielson, Kathleen L., additional, Dong, Yixuan, additional, Hughes, Robert, additional, Haughey, Norman J., additional, Hamilton, James P., additional, and Lutsenko, Svetlana, additional

Published
2022
Full Text
View/download PDF

29. Dendrimer-Conjugated nSMase2 Inhibitor Reduces Tau Propagation in Mice

Author

Tallon, Carolyn, primary, Bell, Benjamin J., additional, Sharma, Anjali, additional, Pal, Arindom, additional, Malvankar, Medhinee M., additional, Thomas, Ajit G., additional, Yoo, Seung-Wan, additional, Hollinger, Kristen R., additional, Coleman, Kaleem, additional, Wilkinson, Elizabeth L., additional, Kannan, Sujatha, additional, Haughey, Norman J., additional, Kannan, Rangaramanujam M., additional, Rais, Rana, additional, and Slusher, Barbara S., additional

Published
2022
Full Text
View/download PDF

32. Neutral sphingomyelinase 2 inhibition attenuates extracellular vesicle release and improves neurobehavioral deficits in murine HIV

Author

Zhu, Xiaolei, primary, Hollinger, Kristen R., additional, Huang, Yiyao, additional, Borjabad, Alejandra, additional, Kim, Boe-Hyun, additional, Arab, Tanina, additional, Thomas, Ajit G., additional, Moniruzzaman, Mohammed, additional, Lovell, Lyndah, additional, Turchinovich, Andrey, additional, Witwer, Kenneth W., additional, Volsky, David J., additional, Haughey, Norman J., additional, and Slusher, Barbara S., additional

Published
2022
Full Text
View/download PDF

33. Immunometabolic Reprogramming in Response to HIV Infection Is Not Fully Normalized by Suppressive Antiretroviral Therapy

Author

Deme, Pragney, primary, Rubin, Leah H., additional, Yu, Danyang, additional, Xu, Yanxun, additional, Nakigozi, Gertrude, additional, Nakasujja, Noeline, additional, Anok, Aggrey, additional, Kisakye, Alice, additional, Quinn, Thomas C., additional, Reynolds, Steven J., additional, Mayanja, Richard, additional, Batte, James, additional, Wawer, Maria J., additional, Sacktor, Ned C., additional, Saylor, Deanna, additional, and Haughey, Norman J., additional

Published
2022
Full Text
View/download PDF

40. Inhibition of neutral sphingomyelinase 2 reduces extracellular vesicle release from neurons, oligodendrocytes, and activated microglial cells following acute brain injury

Author

Tallon, Carolyn, primary, Picciolini, Silvia, additional, Yoo, Seung‐Wan, additional, Thomas, Ajit G., additional, Pal, Arindom, additional, Alt, Jesse, additional, Carlomagno, Cristiano, additional, Gualerzi, Alice, additional, Rais, Rana, additional, Haughey, Norman J., additional, Bedoni, Marzia, additional, and Slusher, Barbara S., additional

Published
2021
Full Text
View/download PDF

42. List of Contributors

Author

Appel, Stanley H., primary, Backonja, Miroslav “Misha”, additional, Bailey, Zinzi D., additional, Beers, David R., additional, Begay, Tommy K., additional, Berti, Anna, additional, Boido, Marina, additional, Boldrini, Maura, additional, Borsook, David, additional, Brown, R.H., additional, Burstein, Rami, additional, Burton, Vera Joanna, additional, Butelman, Eduardo R., additional, Caplan, Louis R., additional, Castellanos, F. Xavier, additional, Cattaneo, Elena, additional, Clemens, Paula R., additional, Cortese, Samuele, additional, Cossetti, Chiara, additional, Coyle, Joseph T., additional, Daneshvar, Daniel H., additional, DeLong, Mahlon R., additional, Feldman, Eva L., additional, Garbarini, Francesca, additional, Gasser, Thomas, additional, Gold, Michael S., additional, Grunze, Heinz, additional, Hagerman, Randi, additional, Harris, James C., additional, Haughey, Norman J., additional, Illes, Judy, additional, Iorio, Raffaele, additional, Ironside, James W., additional, Kaminski, Henry J., additional, Kim, Bhumsoo, additional, Koen, Nastassja, additional, Konopaske, Glenn T., additional, Kornum, Birgitte Rahbek, additional, Kreek, Mary Jeanne, additional, Kristensson, Krister, additional, Kurtzke, John F., additional, Kusner, Linda L., additional, Landsberger, Nicoletta, additional, Li, Tong, additional, Liberski, Paweł P., additional, Lyons, Jennifer L., additional, Maleki, Nasim, additional, Mallucci, Giulia, additional, McArthur, Justin C., additional, McEwen, Bruce S., additional, McKee, Ann C., additional, McKhann, Guy, additional, Melnikova, Tatiana, additional, Mignot, Emmanuel, additional, Miller, Andrew H., additional, Mobley, William C., additional, Neppi-Modona, Marco, additional, O’Toole, Orna, additional, Parsons, Matthew P., additional, Peters, O.M., additional, Picetti, Roberto, additional, Pittock, Sean J., additional, Pluchino, Stefano, additional, Pressman, Peter, additional, Price, Donald L., additional, Raison, Charles L., additional, Raymond, Lynn A., additional, Reed, Brian, additional, Reiner, Peter B., additional, Ressler, Kerry J., additional, Rook, Graham W., additional, Rosen, Howard J., additional, Rowland, Lewis P., additional, Ruparelia, Aarti, additional, Saporta, Mario A., additional, Savonenko, Alena V., additional, Schaeffer, Julia, additional, Scharfman, Helen E., additional, Shapiro, Bruce K., additional, Shy, Michael E., additional, Simon, Roger P., additional, Sims-Robinson, Catrina, additional, Stein, Dan J., additional, Summers, Scott M., additional, Thakur, Kiran T., additional, Tovar-y-Romo, Luis B., additional, Vahabzadeh, Arshya, additional, Vercelli, Alessandro, additional, Wallin, Mitchell T., additional, Wichmann, Thomas, additional, Wiley, Clayton A., additional, Williams, David R., additional, Wong, Philip C., additional, Yuferov, Vadim, additional, Zhao, Weihua, additional, Zigmond, Michael J., additional, and Živković, Saša A., additional

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results