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Your search keyword '"Hau, Samantha J."' showing total 31 results
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31 results on '"Hau, Samantha J."'

7. Evaluation of the recombinant proteins RlpB and VacJ as a vaccine for protection against Glaesserella parasuis in pigs

Author

Hau, Samantha J., Luan, Shi-Lu, Loving, Crystal L., Nicholson, Tracy L., Wang, Jinhong, Peters, Sarah E., Seilly, David, Weinert, Lucy A., Langford, Paul R., Rycroft, Andrew N., Wren, Brendan W., Maskell, Duncan J., Tucker, Alexander W., Brockmeier, Susan L., Brockmeier, Susan L. [0000-0001-8636-3280], and Apollo - University of Cambridge Repository

Subjects
Glaesserella parasuis, Glässer’s disease, Bacteriology, Subunit vaccine, humanities, Research Article
Abstract

Funder: U.S. Department of Agriculture; doi: http://dx.doi.org/10.13039/100000199, Funder: Oak Ridge Institute for Science and Education; doi: http://dx.doi.org/10.13039/100006229, Funder: Department for Environment, Food and Rural Affairs; doi: http://dx.doi.org/10.13039/501100000277, Background: Glaesserella parasuis, the causative agent of Glӓsser’s disease, is widespread in swine globally resulting in significant economic losses to the swine industry. Prevention of Glӓsser’s disease in pigs has been plagued with an inability to design broadly protective vaccines, as many bacterin based platforms generate serovar or strain specific immunity. Subunit vaccines are of interest to provide protective immunity to multiple strains of G. parasuis. Selected proteins for subunit vaccination should be widespread, highly conserved, and surface exposed. Results: Two candidate proteins for subunit vaccination (RlpB and VacJ) against G. parasuis were identified using random mutagenesis and an in vitro organ culture system. Pigs were vaccinated with recombinant RlpB and VacJ, outer membrane proteins with important contributions to cellular function and viability. Though high antibody titers to the recombinant proteins and increased interferon-γ producing cells were found in subunit vaccinated animals, the pigs were not protected from developing systemic disease. Conclusions: It appears there may be insufficient RlpB and VacJ exposed on the bacterial surface for antibody to bind, preventing high RlpB and VacJ specific antibody titers from protecting animals from G. parasuis. Additionally, this work confirms the importance of utilizing the natural host species when assessing the efficacy of vaccine candidates.

Published
2020

9. Generation and Evaluation of a Glaesserella (Haemophilus) parasuis Capsular Mutant

Author

Eberle, Kirsten C., primary, Hau, Samantha J., additional, Luan, Shi-Lu, additional, Weinert, Lucy A., additional, Stasko, Judith A., additional, Wang, Jinhong, additional, Peters, Sarah E., additional, Langford, Paul R., additional, Rycroft, Andrew N., additional, Wren, Brendan W., additional, Maskell, Duncan J., additional, Tucker, Alexander W., additional, and Brockmeier, Susan L., additional

Published
2020
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14. Single Nucleotide Polymorphism Analysis Indicates Genetic Distinction and Reduced Diversity of Swine-Associated Methicillin Resistant Staphylococcus aureus (MRSA) ST5 Isolates Compared to Clinical MRSA ST5 Isolates

Author

Hau, Samantha J., primary, Allué-Guardia, Anna, additional, Rusconi, Brigida, additional, Haan, Jisun S., additional, Davies, Peter R., additional, Frana, Timothy S., additional, Eppinger, Mark, additional, and Nicholson, Tracy L., additional

Published
2018
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17. Interferon alpha inhibits replication of a live-attenuated porcine reproductive and respiratory syndrome virus vaccine preventing development of an adaptive immune response in swine

Author

Brockmeier, Susan L., primary, Loving, Crystal L., additional, Eberle, Kirsten C., additional, Hau, Samantha J., additional, Buckley, Alexandra, additional, Van Geelen, Albert, additional, Montiel, Nestor A., additional, Nicholson, Tracy, additional, and Lager, Kelly M., additional

Published
2017
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31. Generation and Evaluation of a Glaesserella(Haemophilus) parasuisCapsular Mutant

Author

Eberle, Kirsten C., Hau, Samantha J., Luan, Shi-Lu, Weinert, Lucy A., Stasko, Judith A., Wang, Jinhong, Peters, Sarah E., Langford, Paul R., Rycroft, Andrew N., Wren, Brendan W., Maskell, Duncan J., Tucker, Alexander W., and Brockmeier, Susan L.

Abstract

Glaesserella(Haemophilus) parasuisis a commensal bacterium of the upper respiratory tract in pigs and also the causative agent of Glässer’s disease, which causes significant morbidity and mortality in pigs worldwide. Isolates are characterized into 15 serovars by their capsular polysaccharide, which has shown a correlation with isolate pathogenicity. To investigate the role the capsule plays in G. parasuisvirulence and host interaction, a capsule mutant of the serovar 5 strain HS069 was generated (HS069?cap) through allelic exchange following natural transformation.

Published
2020
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