Search

Your search keyword '"Hatori K"' showing total 300 results
Start Over Author "Hatori K"
300 results on '"Hatori K"'

2. Effect of Strain in Channel on Electron Transport Properties of Ga1- xInxSb HEMT Structures with Strained-Al0.40In0.60Sb/Al0.25In0.75Sb Stepped Buffer

Author

Hatori, K., primary, Kishimoto, N., additional, Hiraoka, M., additional, Endoh, Y., additional, Osawa, K., additional, Hayashi, T., additional, Kemmochi, Y., additional, Machida, R., additional, Watanabe, I., additional, Yamashita, Y., additional, Hara, S., additional, Kasamatsu, A., additional, Endoh, A., additional, and Fujishiro, H. I., additional

Published
2022
Full Text
View/download PDF

13. Epstein–Barr virus reactivation by persistent apical periodontal pathogens.

Author

Himi, K., Takeichi, O., Imai, K., Hatori, K., Tamura, T., and Ogiso, B.

Subjects
EPSTEIN-Barr virus diseases, VIRUS reactivation, IN vitro studies, IN vivo studies, POLYMERASE chain reaction, GENE expression, PERIODONTITIS
Abstract

Aim: To assess whether Epstein–Barr virus (EBV) reactivation is triggered by persistent apical periodontitis‐related microbes using in vitro and ex vivo methodologies. Methodology: Surgically removed human periapical granulomas (n = 50) and healthy gingival tissues (n = 10) were analysed to determine the presence of EBV and seven persistent apical periodontitis‐related microbes. In addition, real‐time polymerase chain reaction was used to detect the mRNA expression of BZLF‐1, an immediate–early gene of EBV. Expression of latent membrane protein (LMP)‐1 and ZEBRA, an early lytic protein of EBV encoded by BZLF‐1, was also examined using triple‐colour immunofluorescence staining. n‐Butyric acid produced by the microbes was quantified, and luciferase assays were performed in association with bacterial lysates. In addition, Daudi cells were cultured with bacterial lysates, and the expression levels of BZLF‐1 mRNA and ZEBRA protein were determined. Results: EBV DNA and BZLF‐1 mRNA were detected in 47 out of 50 periapical granulomas, but not in healthy gingival tissues. The EBV DNA copy number and the number of Fusobacterium nucleatum were significantly positively correlated with BZLF‐1 expression in periapical granulomas. The number of Prevotella intermedia was slightly correlated with BZLF‐1 expression; however, the other microbes were not. CD79a‐positive B cells in periapical granulomas, but not those in healthy gingival tissues, expressed both LMP‐1 and ZEBRA. n‐Butyric acid production was the highest in F. nucleatum and the lowest in P. intermedia. Enterococcus faecalis, Candida albicans and the other tested microbes did not produce n‐butyric acid. An F. nucleatum lysate exhibited significantly increased BZLF‐1‐luciferase activity in the same manner of commercial butyric acid, whereas P. intermedia did not. F. nucleatum also induced the expression of BZLF‐1 mRNA and ZEBRA protein by Daudi cells, indicating that EBV reactivation was induced. Conclusion: Among the persistent apical periodontitis‐related bacteria that were tested, F. nucleatum most strongly reactivated latent EBV, whereas E. faecalis and C. albicans as well as the other microbes did not. [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
View/download PDF

19. Porphyromonas endodontalis reactivates latent Epstein–Barr virus.

Author

Makino, K., Takeichi, O., Imai, K., Inoue, H., Hatori, K., Himi, K., Saito, I., Ochiai, K., and Ogiso, B.

Subjects
PORPHYROMONAS gingivalis infections, EPSTEIN-Barr virus, SHORT-chain fatty acids, ENDODONTICS, TRANSCRIPTION factors, LUCIFERASES, HISTONE acetylation, PERIAPICAL granuloma
Abstract

Aim: To determine whether Porphyromonas endodontalis can reactivate latent Epstein–Barr virus (EBV). Methodology: The concentrations of short‐chain fatty acids (SCFAs) in P. endodontalis culture supernatants were determined using high‐performance liquid chromatography. A promoter region of BamHI fragment Z leftward open reading frame 1 (BZLF‐1), which is a transcription factor that controls the EBV lytic cycle, was cloned into luciferase expression vectors. Then, the luciferase assay was performed using P. endodontalis culture supernatants. Histone acetylation using Daudi cells treated with P. endodontalis culture supernatants was examined using Western blotting. BZLF‐1 mRNA and BamHI fragment Z EB replication activator (ZEBRA) protein were also detected quantitatively using real‐time polymerase chain reaction (PCR) and Western blotting. Surgically removed periapical granulomas were examined to detect P. endodontalis, EBV DNA, and BZLF‐1 mRNA expression using quantitative real‐time PCR. Statistical analysis using Steel tests was performed. Results: The concentrations of n‐butyric acid in P. endodontalis culture supernatants were significantly higher than those of other SCFAs (P = 0.0173). Using B‐95‐8‐221 Luc cells treated with P. endodontalis culture supernatants, the luciferase assay demonstrated that P. endodontalis induced BZLF‐1 expression. Hyperacetylation of histones was also observed with the culture supernatants. BZLF‐1 mRNA and ZEBRA protein were expressed by Daudi cells in a dose‐dependent manner after the treatment with P. endodontalis culture supernatants. P. endodontalis and BZLF‐1 in periapical granulomas were also detected. The expression levels of BZLF‐1 mRNA were similar to the numbers of P. endodontalis cells in each specimen. Conclusions: n‐butyric acid produced by P. endodontalis reactivated latent EBV. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
View/download PDF

20. EXCITATION OF ICRF WAVES IN RFC-XX DEVICE

Author

Adati, K., primary, Aoki, T., additional, Hidekuma, S., additional, Hatori, K., additional, Hiroe, S., additional, Ichimura, M., additional, Kawamoto, T., additional, Kumazawa, R., additional, Okubo, Y., additional, Okamura, S., additional, Sato, T., additional, Watari, T., additional, and Singh, C.M., additional

Published
1982
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results