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32 results on '"Hassink, Gerco"'

7. Neuroprotective effect of hypoxic preconditioning and neuronal activation in a in vitro human model of the ischemic penumbra

Author

Pires Monteiro, Sara, primary, Voogd, Eva, additional, Muzzi, Lorenzo, additional, De Vecchis, Gianmarco, additional, Mossink, Britt, additional, Levers, Marloes, additional, Hassink, Gerco, additional, Van Putten, Michel, additional, Le Feber, Joost, additional, Hofmeijer, Jeannette, additional, and Frega, Monica, additional

Published
2021
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13. Ischemic Stroke: Treatments to Improve Neuronal Functional Recovery in vitro

Author

Pires Monteiro, Sara, Covelo, Joana, Levers, Marloes, Hassink, Gerco, le Feber, Joost, Frega, Monica, Blobel, Bernd, Giacomini, Mauro, and Clinical Neurophysiology

Subjects
Ischemic stroke, rodent, iPSCs, neuronal network, penumbra, electrical stimulation
Abstract

The understanding of the neurophysiological processes that occur in the areas that surround the core of a brain infarct is crucial for the creation of new therapies and treatments to improve neuronal recovery. The present study aims to demonstrate that both rodent and human neuronal networks lose their activity under low oxygen conditions and that electrical stimulation can increase the probability of recovery. Hypoxia was induced in rodent and human neurons and the effects of electrical stimulation were assessed in the rat cultures. The results obtained show that neuronal activation, in the form of electrical stimulation, has the potential to maintain the networks at higher levels of activity and, therefore, to improve cell survival. This study will open the way for new treatment strategies based on brain-stimulation to enhance neuronal recovery and will be of large relevance for patients, families, and society.

Published
2019

21. Functionally different α-synuclein inclusions yield insight into Parkinson’s disease pathology

Author

Raiss, Christian C., primary, Braun, Theresa S., additional, Konings, Irene B. M., additional, Grabmayr, Heinrich, additional, Hassink, Gerco C., additional, Sidhu, Arshdeep, additional, le Feber, Joost, additional, Bausch, Andreas R., additional, Jansen, Casper, additional, Subramaniam, Vinod, additional, and Claessens, Mireille M. A. E., additional

Published
2016
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24. Epstein-Barr Virus Encodes Three Bona Fide Ubiquitin-Specific Proteases

Author

Sompallae, Ramakrishna, Gastadello, Stefano, Hildebrand, Sebastian, Zinin, Nikolay, Hassink, Gerco, Lindsten, Kristina, Haas, Jürgen, Masucci, Maria G, Persson, Bengt, Sompallae, Ramakrishna, Gastadello, Stefano, Hildebrand, Sebastian, Zinin, Nikolay, Hassink, Gerco, Lindsten, Kristina, Haas, Jürgen, Masucci, Maria G, and Persson, Bengt

Published
2008
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29. Nanoscaffold's stiffness affects primary cortical cell network formation.

Author

Sijia Xie, Schurink, Bart, Wolbers, Floor, Luttge, Regina, and Hassink, Gerco

Subjects
BRAIN physiology, NANOPATTERNING, EXTRACELLULAR matrix, POLYDIMETHYLSILOXANE, CELL culture, ASTROCYTES
Abstract

Networks of neurons cultured on-chip can provide insights into both normal and disease-state brain function. The ability to guide neuronal growth in specific, artificially designed patterns allows us to study how brain function follows form. Primary cortical cells cultured on nanograting scaffolds, in particular astrocytes, showed highly ordered regions of dendritic outgrowth. Usually, materials suitable for nanopatterning have a stiffness far above that of the extracellular matrix. In this paper, the authors studied two materials with large differences in stiffness, polydimethylsiloxane (PDMS) and silicon. Our results show that both nanopatterned silicon and PDMS guide the outgrowth of astrocytes in cortical cell culture, but the growth of the astrocyte is affected by the stiffness of the substrate, as revealed by differences in the cell soma size and the organization of the outgrowth. [ABSTRACT FROM AUTHOR]

Published
2014
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30. Rat Cytomegalovirus Induces a Temporal Downregulation of Major Histocompatibility Complex Class I Cell Surface Expression

Author

Hassink, Gerco C., Duijvestijn-Van Dam, Joanne G., Koppers-Lalic, Danijela, Den Brink, Jacqueline Van Gaans-Van, Leeuwen, Daphne Van, Vink, Cornelis, Bruggeman, Cathrien A., and Wiertz, Emmanuel J.H.J.

Abstract

Herpesviruses are known to influence expression of major histocompatibility complex (MHC) class I molecules on the surface of infected cells using a variety of mechanisms. Downregulation of MHC class I expression prohibits detection and elimination of infected cells by cytotoxic T lymphocytes. To investigate the effect of rat cyomegalovirus (RCMV) infection on MHC class I expression, we infected immortalized and primary rat fibroblasts with RCMV and monitored surface expression of MHC class I molecules at various time-points postinfection. These experiments revealed a downregulation of MHC class I surface expression by RCMV, a phenomenon that has also been reported for human and murine CMV. However, in contrast to the other cytomegaloviruses, RCMV causes only a temporal downregulation of MHC class I, with a maximal decrease at 12 h postinfection. Unlike murine and human CMV, RCMV does not induce proteolytic degradation of MHC class I molecules. In RCMV-infected cells, the MHC class I molecules are stable, but their exit from the ER is delayed.

Published
2005
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31. SCFTrCP acts in endosomal sorting of the GH receptor

Author

van Kerkhof, Peter, Westgeest, Marijn, Hassink, Gerco, and Strous, Ger J.

Subjects
*ENDOSOMES, *HORMONE receptors, *UBIQUITIN, *LIGASES, *EPIDERMAL growth factor, *ERYTHROPOIETIN, *SMALL interfering RNA, *PROTEIN-tyrosine kinases
Abstract

Abstract: The ubiquitin ligase SCFTrCP is required for internalisation of the growth hormone receptor (GHR) and acts via a direct interaction with the ubiquitin-dependent endocytosis motif. Details of how the ligase communicates its information to the clathrin-mediated internalisation machinery are unknown. For the EGF receptor, c-Cbl acts both at the cell surface and in endosomes. We hypothesised that SCFTrCP is required for GHR degradation at both sites. This was tested by truncating GHR after a di-leucine-based internalisation motif (GHR349). This receptor enters the cells via the adapter complex AP2. We show that TrCP acts in an early stage of cargo selection: both TrCP silencing and mutation of the ubiquitin-dependent endocytosis motif force the GHR to recycle between endosomes and the plasma membrane, together with the transferrin receptor. Depletion of Tsg101 (ESCRT-I) has the same effect, while silencing of Hrs (ESCRT-0) prevents GH recycling. GH passes through late endosomal vesicles, marked by Lamp1. Coexpressing GHR and EGFR demonstrates that both receptors use the same route to the lysosomes. We show for the first time that SCFTrCP is involved in cargo-specific sorting at endosomes and that Tsg101 rather than Hrs might direct the cargo into the ESCRT machinery. [Copyright &y& Elsevier]

Published
2011
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32. Ischemic Stroke: Treatments to Improve Neuronal Functional Recovery in vitro.

Author

Pires Monteiro S, Covelo J, Levers M, Hassink G, le Feber J, and Frega M

Subjects
Animals, Humans, Hypoxia, Neurons, Rats, Brain Ischemia rehabilitation, Recovery of Function, Stroke
Abstract

The understanding of the neurophysiological processes that occur in the areas that surround the core of a brain infarct is crucial for the creation of new therapies and treatments to improve neuronal recovery. The present study aims to demonstrate that both rodent and human neuronal networks lose their activity under low oxygen conditions and that electrical stimulation can increase the probability of recovery. Hypoxia was induced in rodent and human neurons and the effects of electrical stimulation were assessed in the rat cultures. The results obtained show that neuronal activation, in the form of electrical stimulation, has the potential to maintain the networks at higher levels of activity and, therefore, to improve cell survival. This study will open the way for new treatment strategies based on brain-stimulation to enhance neuronal recovery and will be of large relevance for patients, families, and society.

Published
2019

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