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The construction industry plays a crucial role in the global economy, but its heavy reliance on forest resources has led to significant environmental concerns, such as deforestation and climate change. The growing demand for sustainable and affordable building materials has driven researchers to explore the potential of agro-industrial wastes as alternative raw materials for particleboard (PB) production. This study aims to contribute to the growing body of knowledge on sustainability in the built environment by examining the trends, key factors, and environmental implications of utilizing agro waste in particleboard manufacturing through a comprehensive review of recent research. Utilizing the PRISMA approach, this study selects and systematically reviews 50 journal articles and conference papers from Scopus database, published between 2000 and 2024. Research findings reveals a diverse range of agro waste such as sugarcane bagasse, rice husks, corn stover, peanut shells, wheat straws and coconut fibers etc, been successfully used as raw materials for particleboards manufacturing. The bibliometric analysis highlights present focus on production processes, adhesive systems, particle properties, and material optimization. Also, the performance of agro based particleboards is influenced by the type and proportion of agro waste, particle size and geometry, adhesive type and content, and processing conditions. While the use of synthetic resins currently dominates the research landscape as binders, there is growing appetite for bio-based and natural adhesives. However, challenges such as seasonal availability and the need for additional processing must be addressed for large-scale adoption. The study concludes with recommendations for collaborative research, eco-friendly adhesive development, and supportive policies to promote the use of agro waste particleboards in sustainable construction.

Introduction: The process of green synthesis of metal nanoparticles is considered to be eco-friendly and cost-effective. Methods: In this study, bimetallic Ag@Se-P and Ag@Se-S nanoparticles were synthesized successfully using Parkinsonia aculeata aerial parts and seed extracts. The phytochemical contents in P. aculeata aerial parts and seed aqueous extract serve as reducing and stabilizing capping agents without the need for any chemical stabilization additive in the synthesis of bimetallic nanoparticles. Result and Discussion: The obtained results from UV-vis spectrophotometry, scanning electron microscopy (SEM), X-ray powder diffraction (XRD), energy-dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FT-IR) confirmed the successful synthesis of bimetallic nanoparticles with cluster irregular spherical morphology, crystalline nature, and average particle sizes of 17.65 and 24.36 nm for Ag@Se-S and Ag@Se-P, respectively. The cytotoxicity assessment of greenly synthesized nanomaterials using seed and plant extracts showed cell inhibition >50 μg/mL. Ag@Se-S and Ag@Se-P seed and plant extracts significantly reduced LPS-induced inflammation, which was assessed by NO and cytokines IL-1β, IL-6, and TNF-α. The mRNA and protein expression levels of phosphoinositide 3 kinase (PI3K) and nuclear factor kappa B (NFkB) were significantly overexpressed in LPS-induced RAW 264.7 cell lines. Ag@Se-S and Ag@Se-P downregulated the expression of PI3K and NFkB in LPS-induced cell models., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Hassanin, Taha, Ibrahim, Ahmed, Mohamed and Ahmed.)

In the present work, the facile eco-friendly synthesis and evaluation of the anti-tumor activity of Ni(OH) 2 @Mn 3 O 4 nanocomposite were carried out. The synthesis of Ni(OH) 2 @Mn 3 O 4 nanocomposite from chia-seed extract was mediated by sonication. The obtained materials were characterized by different spectroscopic techniques such as transmission electron microscopy (TEM), scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), ultraviolet-visible (UV-Vis), and Fourier transform infrared (FT-IR) spectroscopies. The results of XRD, SEM, EDS, TEM, FT-IR, and UV-Vis analysis indicate the successful manufacturing of a crystalline, cactus-type Ni(OH) 2 @Mn 3 O 4 nanocomposite of 10.10 nm average particle size. XPS analysis confirms that the synthesized materials consist mainly of Ni 2+ , Mn 2+ , and Mn 3+ . The antitumor activity of the nanocomposite was tested against a breast cancer (MCF-7) cell line. The results showed Ni(OH) 2 @Mn 3 O 4 nanocomposite possesses insignificant cytotoxicity. The cell-death percentage was 34% at a 100 ppm concentration of Ni(OH) 2 @Mn 3 O 4 nanocomposite. The obtained results imply that the synthesized nanocomposite could be suitable and safe for drug delivery and water treatment., Competing Interests: The authors declare no conflict of interest.

Theophrasite β-Ni(OH) 2 nanocluster were fabricated via the sonochemical-assisted biogenic method using chia seeds extract as a reducing and stabilizing agent. The optical and morphological feature of the synthesized nanocluster was characterized using UV-Vis, FTIR, FE-SEM-EDS, HR-TEM, DLS, XPS, and XRD analysis. According to FE-SEM and HR-TEM images of the synthesized materials, β-Ni(OH) 2 nanocluster illustrates the hexagonal particle shape with an average size of 5.8 nm, while the EDS results confirm the high purity of the synthesized nanocluster. Moreover, the XRD pattern of the synthesized materials shows typical peaks that match the reference pattern of the Theophrasite form of β-Ni(OH) 2 with a hexagonal crystal system. The XPS analysis illustrates that the prepared samples exhibit both Ni 2+ and Ni 3+ with the predominance of Ni 2+ species. Additionally the in-vitro cytotoxic activity of β-Ni(OH) 2 nanocluster is tested against the MCF7 cell lines (breast cancer cells). The MTT assay results proved that the synthesized β-Ni(OH) 2 nanocluster has potent cytotoxic activity against breast cancer cell lines (IC 50 : 62.7 μg/mL).

Phosphorylase is a type of enzyme-producing sugar phosphates through the reversible phosphorolysis reactions of glycosides, which makes it an important starting enzyme in multi-enzyme systems for rare sugar biomanufacturing. To investigate its application in D-tagatose biosynthesis from maltodextrin using in vitro multi-enzyme cascade biosystem, the α-glucan phosphorylase (αGP; EC 2.4.1.1) from the thermophile D. turgidum DSM 6724 was prepared and characterized. It exhibited the specific activity of 30.28 U/mg at its optimal temperature of 70 °C. Thermostability results revealed that DituαGP could maintain more than 25% of initial activity for 4 h, even at 90 °C. The highest activity was observed at pH 5.5, and most divalent metal ions deactivated the enzyme. DituαGP exhibited great application potential in the multi-enzyme system that about 3.919 g/L of D-tagatose was produced from 150 g/L of maltodextrin within 36 h. DituαGP has played an important role in this biosystem and will also be applied in the synthesis of other rare sugars from maltodextrin., (© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Background: Gracilibacillus alcaliphilus SK51.001, a strain that produces β-CGTase (β-cyclodextrin glucanotransferase) (EC 2.4.1.19), was screened and isolated from Sudanese soil. The objective of this study was to sequence and characterize the β-CGTase gene from G. alcaliphilus SK51.001., Results: According to 16S rRNA analysis of the strain and its morphological shape, it was identified as G. alcaliphilus. The β-CGTase gene was successfully cloned, sequenced, and expressed in Escherichia coli BL21. This gene showed 706 amino acid residues including 33 amino acids as a signal peptide. The active site residues of G. alcaliphilus SK51.001CGTase were described using enzyme modeling and docking with the products. The estimated molecular mass of G. alcaliphilus SK51.001CGTase was approximately 74 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the evaluation of the gel filtration showed approximately 85 kDa, which means G. alcaliphilus SK51.001CGTase is a monomer. The optimum temperature and pH of G. alcaliphilus SK51.001CGTase were 60 °C and 7.0 respectively. Gracilibacillus alcaliphilus SK51.001CGTase was comparatively stable at a pH levels between 6.0 and 9.0 and temperatures of 30-50 °C. The activity of G. alcaliphilus SK51.001CGTase was increased by Ni 2+ , and Co 2+ but inhibited by Al 3+ and Fe 3+ . The kinetic parameters of K m and V max were 2068.52 μg mL -1 and 0.13 μmol mL -1  min -1 , respectively., Conclusion: Gracilibacillus alcaliphilus SK51.001CGTase could hydrolyze soluble starch into α-, β-, and γ-cyclodextrin in a ratio of 2: 83: 15% respectively. This high ratio production of β-CD could allow the enzyme to be used in β-CD production. © 2020 Society of Chemical Industry., (© 2020 Society of Chemical Industry.)

Tagatose is a rare hexoketose with potential health benefits. Here, an enzyme, GatZ subunit ofd-tagatose-1,6-bisphosphate aldolase, was characterized. GatZ is involved in a multi-enzyme cascade reaction system that can produce tagatose from maltodextrin. It showed maximum activity at 70 °C and a pH 8.0, and required supplementation with 5 mM Mg 2+ to achieve the highest catalytic activity. The K m and V max values of GatZ using fructose 6-phosphate as substrate were 5.66 mM and 0.0329 mmol/L min, respectively. An in vitro multi-enzyme system containing GatZ was constructed, and 1.75 g/L tagatose was produced from 5 g/L maltodextrin after 10 h. This biosystem could potentially enrich the application of C4 epimerases in rare sugar bioproduction., (Copyright © 2020 Elsevier Inc. All rights reserved.)

Background: Lactulose has various uses in the food and pharmaceutical fields. Thermostable enzymes have many advantages for industrial exploitation, including high substrate solubilities as well as reduced risk of process contamination., Results: Enzymatic synthesis of lactulose employing a transgalactosylation reaction by a recombinant thermostable glycoside hydrolase (GH1) from the hyperthermophilic archaeon Caldivirga maquilingensis IC-167 was investigated. The optimal pH for lactulose production was found to be 4.5, while the optimal temperature was 85 °C, before it dropped moderately to 83% at 90 °C. However, the relative activity for lactulose synthesis dropped sharply to 35% at 95 °C. At optimal reaction conditions of 70% (w/w) initial sugar substrates with molar ratio of lactose to fructose of 1:4, 15 U mL -1 enzyme concentration and 85 °C, the time course reaction produced a maximum lactulose concentration of 108 g L -1 at 4 h, corresponding to a lactulose yield of 14% and 27 g L -1  h -1 productivity with 84% lactose conversion. The transgalactosylation reaction for lactulose synthesis was greatly influenced by the ratio of galactose donor to acceptor., Conclusion: This novel GH1 may be useful for process applications owing to its high activity in very concentrated substrate reaction media and promising thermostability. © 2017 Society of Chemical Industry., (© 2017 Society of Chemical Industry.)

Background: Hyperthermophilic archaea capable of functioning optimally at very high temperatures are a good source of unique and industrially important thermostable enzymes., Results: A glycoside hydrolase family 1 β-galactosidase gene (BglB) from a hyperthermophilic archaeon Caldivirga maquilingensis IC-167 was cloned and expressed in Escherichia coli. The recombinant enzyme (CMbg0408) displayed optimum activity at 110 °C and pH 5.0. It also retained 92% and 70% of its maximal activity at 115 and 120 °C, respectively. The enzyme was completely thermostable and active after 120 min of incubation at 80 and 90 °C. It also showed broad substrate specificity with activities of 8876 ± 185 U mg -1 for p-nitrophenyl-β-d-galactopyranoside, 4464 ± 172 U mg -1 for p-nitrophenyl-β-d-glucopyranoside, 1486 ± 68 U mg -1 for o-nitrophenyl-β-d-galactopyranoside, 2250 ± 86 U mg -1 for o-nitrophenyl-β-d-xylopyranoside and 175 ± 4 U mg -1 for lactose. A catalytic efficiency (k cat /K m ) of 3059 ± 122 mmol L -1  s -1 and K m value of 8.1 ± 0.08 mmol L -1 were displayed towards p-nitrophenyl-β-d-galactopyranoside., Conclusion: As a result of its remarkable thermostability and high activity at high temperatures, this novel β-galactosidase may be useful for food and pharmaceutical applications. © 2016 Society of Chemical Industry., (© 2016 Society of Chemical Industry.)

Background: A new ribitol dehydrogenase gene was cloned from Providencia alcalifaciens RIMD 1656011 and expressed in Escherichia coli BL21. This study aimed to purify and characterize the ribitol dehydrogenase from P. alcalifaciens RIMD 1656011 and investigate its substrate specificity for potential use as an industrial enzyme., Results: The protein was purified by nickel affinity chromatography. The molecular mass of the purified enzyme was determined as ∼25 000 and 26 650 Da through sodium dodecyl sulfate polyacrylamide gel electrophoresis and liquid chromatography/mass spectrometry respectively. The result for native molecular mass (104 kDa) suggested that the enzyme functions as a tetramer. Optimum activity of the enzyme was determined at pH 10.0 and a temperature of 35 °C. Regarding its thermal stability, the enzyme retained 72, 72, 48 and 0% of its initial activity after 4 h at 25, 30, 40 and 50 °C respectively. The Km , kcat and kcat /Km values of the enzyme for the substrate ribitol were determined as 13.9 mmol L(-1) , 10.0 s(-1) and 0.71 L mmol(-1) s(-1) respectively. The Km of NAD(+) was 0.042 mmol L(-1) ., Conclusion: The substrate specificity indicated that the ribitol dehydrogenase from P. alcalifaciens RIMD 1656011 can be used for direct production of allitol from d-fructose without any by-product formation. © 2015 Society of Chemical Industry., (© 2015 Society of Chemical Industry.)

The efficiency of singlet oxygen photosensitized by some ruthenium(ii) bipyridyl complex ions in aqueous media is reported in this study. Measurements were carried out in H2O and D2O. The effect of the deuterium isotope on the lifetime of (3)MLCT excited states of these complexes is studied in H2O and D2O. The deuterium isotope effect was discussed in terms of the vibronic coupling to the solvent in addition to the charge transfer to the solvent mechanism due to their dependence on the oxidation potential of the sensitizer. Quenching rate constants, kq, for quenching of the (3)MLCT states of these ruthenium complex ions by molecular oxygen were found to be in the range of (2.08-3.84) × 10(9) M(-1) s(-1) in H2O and (1.69-3.48) × 10(9) M(-1) s(-1) in D2O. The efficiency of singlet oxygen, O2((1)Δg), production as a result of the (3)MLCT quenching by oxygen, f, is reported in D2O and found to be in the range 0.25-0.56. It has been found that the lifetime of the excited state is longer in D2O, τ, than in H2O, τ, which was related to partial charge transfer to the solvent in addition to the vibronic coupling mechanism. Mechanisms by which the excited states of these ruthenium complexes are quenched by molecular oxygen that shows the competition between charge transfer, non-charge transfer deactivation channels or energy transfer assisted charge transfer deactivation mechanisms are reported.

We report the first studies on the reaction between an HNO donor compound and vitamin B12 complexes. Kinetic and mechanistic studies have been carried out on the reaction between the vitamin B12 derivative aquacobalamin (H2OCbl(+)/HOCbl; pKa = 7.8) and the HNO donor Angeli's salt. Studies were carried out with aquacobalamin in excess, since nitrite also reacts with aquacobalamin to form nitrocobalamin (NO2Cbl). At pH <9.90 aquacobalamin reacts directly with the monoprotonated form of Angeli's salt, HN2O3(-), to form nitroxylcobalamin (NO(-)-Cbl(III); NOCbl) and nitrite. At pH >10.80 the reaction instead switches predominantly to a mechanism in which spontaneous decomposition of Angeli's salt to give HNO and nitrite becomes the rate-determining step, followed by the rapid reaction between aquacobalamin and HNO/NO(-) to again give NOCbl. Both reactions proceed with a 1:1 stoichiometry and formation of nitrite is confirmed using the Griess assay.

The X-ray structures of three new crystals of nitroxylcobalamin (NOCbl) have been determined. Unlike our earlier reported structure in which NOCbl was partially oxidized (L. Hannibal, C. A. Smith, D. W. Jacobsen and N. E. Brasch, Angew. Chem., Int. Ed. 2007, 46, 5140), the O atom of the nitroxyl ligand is located in a single position with a N=O bond distance of 1.12-1.14 Å, consistent with a double bond. The Co-N-O angle is in the 118.9-120.3 Å range. The α-axial Co-N(dimethylbenzimidazole) (Co-NB3) bond distance is a remarkable 2.32-2.35 Å in length, ~0.1 Å longer than that reported for all other cobalamin structures. The change in the Gibbs free energy for the base-on/base-off equilibrium now correlates extremely well with the Co-NB3 bond distance, as observed for other cobalamins.

The structure of nitrosylcobalamin (NOCbl) in solution has been studied by NMR spectroscopy and the 1H and 13C NMR spectra have been assigned. 13C and 31P NMR chemical shifts, the UV-vis spectrum of NOCbl and the observed pKbase-off value of approximately 5.1 for NOCbl provide evidence that a significant fraction of NOCbl is present in the base-off, 5,6-dimethylbenzimidazole (DMB) deprotonated, form in solution. NOE-restrained molecular mechanics modelling of base-on NOCbl gave annealed structures with minor conformational differences in the flexible side chains and the nucleotide loop position compared with the X-ray structure. A molecular dynamics simulation at 300 K showed that DMB remains in close proximity to the alpha face of the corrin in the base-off form of NOCbl. Simulated annealing calculations produced two major conformations of base-off NOCbl. In the first, the DMB is perpendicular to the corrin and its B3 nitrogen is about 3.1 A away from and pointing directly at the metal ion; in the second the DMB is parallel to and tucked beneath the D ring of the corrin.

Accurate determination of vanadium (V) in industrial waste water is of great importance in environmental, biological and toxicological studies. Most of kinetic spectrophotometric methods based on the catalytic effect of vanadium (V), when applied to real samples for determination of trace levels of vanadium (V) lack the satisfactory sensitivity and selectivity. This may be attributed to the serious interferences of various anions which are common pollutants in industrial waste water. The oxidation of gallic acid by ammonium persulphate, catalysed by vanadium (V) was chosen for our study. The effect of the serious interferences of various anions such as chloride, bromate, bromide, chromate, iodide, iodate, molybdate, carbonate and sulphate on the net absorbance given by 4 microg l(-1) of vanadium (V) solution were studied. The minimum concentrations of citric acid, EDTA, ascorbic acid and oxalic acid as leveling off agents required to level off interfering effects due to the aforementioned anions in the kinetic determination of vanadium (V) were 50, 70, 80 and 120 microg ml(-1), respectively. In the presence of optimum concentrations of effective leveling off agents, the dynamic range can be extended and sensitivity increased as compared with the proposed method without levelling off agents. The proposed method is a rapid, sensitive and selective method for the determination of ultra trace amounts of vanadium (V) in real samples with satisfactory results.