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425 results on '"Hasegawa, Tomomi"'

4. Genetic identification and antimicrobial susceptibility of clinically isolated anaerobic bacteria: A prospective multicenter surveillance study in Japan

Author

Fujita, Naohisa, Komori, Toshiaki, Yamada, Yukiji, Shimizu, Tsunehiro, Hayashi, Akihiko, Ono, Tamotsu, Fukuda, Osamu, Watanabe, Harumi, Ichiyama, Satoshi, Higuchi, Takeshi, Takakura, Shunji, Nagao, Miki, Matsumura, Yasufumi, Yamamoto, Masaki, Moro, Kunihiko, Hasegawa, Tomomi, Yoshida, Takashi, Shigeta, Masayo, Terada, Hiromi, Fukumoto, Chiyoko, Mitamura, Yoshie, Tsuboi, Fusayuki, Yunoki, Tomoyuki, Tanaka, Michio, Hamano, Kyoko, Nakano, Satoshi, and Noguchi, Taro

Published
2017
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6. Mutation of OUR1/OsbZIP1, which encodes a member of the basic leucine zipper transcription factor family, promotes root development in rice through repressing auxin signaling

Author

Hasegawa, Tomomi, Lucob-Agustin, Nonawin, Yasufuku, Koki, Kojima, Takaaki, Nishiuchi, Shunsaku, Ogawa, Atsushi, Takahashi-Nosaka, Misuzu, Kano-Nakata, Mana, Inari-Ikeda, Mayuko, Sato, Moeko, Tsuji, Hiroyuki, Wainaina, Cornelius Mbathi, Yamauchi, Akira, Inukai, Yoshiaki, Hasegawa, Tomomi, Lucob-Agustin, Nonawin, Yasufuku, Koki, Kojima, Takaaki, Nishiuchi, Shunsaku, Ogawa, Atsushi, Takahashi-Nosaka, Misuzu, Kano-Nakata, Mana, Inari-Ikeda, Mayuko, Sato, Moeko, Tsuji, Hiroyuki, Wainaina, Cornelius Mbathi, Yamauchi, Akira, and Inukai, Yoshiaki

Abstract

A well-developed root system is essential for efficient water uptake, particularly in drought-prone environments. However, the molecular mechanisms underlying the promotion of root development are poorly understood. We identified and characterized a rice mutant, outstanding rooting1 (our1), which exhibited a well-developed root system. The our1 mutant displayed typical auxin-related phenotypes, including elongated seminal root and defective gravitropism. Seminal root elongation in the our1 mutant was accelerated via the promotion of cell division and elongation. In addition, compared with the wild type, the density of short and thin lateral roots (S-type LRs) was reduced in the our1 mutant, whereas that of long and thick LRs (L-type LRs) was increased. Expression of OUR1, which encodes OsbZIP1, a member of the basic leucine zipper transcription factor family, was observed in the seminal root tip and sites of LR emergence, wherein attenuation of reporter gene expression levels controlled by the auxin response promoter DR5 was also observed in the our1 mutant. Taken together, our results indicate that the our1 gene promotes root development by suppressing auxin signaling, which may be a key factor contributing to an improvement in root architecture.

Published
2023

14. Therapeutic applications of local injection of hsa-miR-634into canine spontaneous malignant melanoma tumors

Author

Yoshikawa, Ryutaro, Inoue, Jun, Iwasaki, Ryota, Terauchi, Mitsuhiko, Fujii, Yuji, Ohta, Maya, Hasegawa, Tomomi, Mizuno, Rui, Mori, Takashi, and Inazawa, Johji

Abstract

Malignant melanoma (MM) is one of the most common tumors in both dogs and humans. As canine MM (CMM) and human MM (HMM) have similar clinical characteristics, CMM appears to be a good clinical model for HMM. We previously demonstrated that the introduction of a synthetic double-strand-microRNA-634 (miR-634) mimic triggered apoptotic cell death by directly targeting the genes associated with cytoprotective processes in various human cancer cell lines, including those of HMM. This study aimed to investigate the antitumor effects of the local administration of miR-634on spontaneous CMMs to provide a basis for future applications of miR-634formulations in HMM treatment. We found that miR-634administration induced apoptosis in CMM cell lines in vitro via downregulation of Asct2, Nrf2, and survivin expression, similar to the mechanisms in HMM cell lines. Furthermore, intratumoral miR-634administration induced antitumor effects in four of seven spontaneous CMM cases, with no adverse effects. Local administration of miR-634to lung metastasis under ultrasound guidance induced tumor shrinkage. These results confirm the antitumor effect of the local administration of miR-634in spontaneous CMM, a model for spontaneous HMM, thereby providing a novel treatment strategy for HMM.

Published
2023
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15. The promoted lateral root 1 (plr1) mutation is involved in reduced basal shoot starch accumulation and increased root sugars for enhanced lateral root growth in rice

Author

Lucob-Agustin, Nonawin, Sugiura, Daisuke, Kano-Nakata, Mana, Hasegawa, Tomomi, Suralta, Roel R., Niones, Jonathan M., Inari-Ikeda, Mayuko, Yamauchi, Akira, Inukai, Yoshiaki, Lucob-Agustin, Nonawin, Sugiura, Daisuke, Kano-Nakata, Mana, Hasegawa, Tomomi, Suralta, Roel R., Niones, Jonathan M., Inari-Ikeda, Mayuko, Yamauchi, Akira, and Inukai, Yoshiaki

Abstract

Lateral roots (LRs) are indispensable for plant growth, adaptability and productivity. We previously reported a rice mutant, exhibiting a high density of thick and long LRs (L-type LRs) with long parental roots and herein referred to as promoted lateral root1 (plr1). In this study, we describe that the mutant exhibited decreased basal shoot starch accumulation, suggesting that carbohydrates might regulate the mutant root phenotype. Further analysis revealed that plr1 mutation gene regulated reduced starch accumulation resulting in increased root sugars for the regulation of promoted LR development. This was supported by the exogenous glucose application that promoted L-type LRs. Moreover, nitrogen (N) application was found to reduce basal shoot starch accumulation in both plr1 mutant and wild-type seedlings, which was due to the repressed expression of starch biosynthesis genes. However, unlike the wild-type that responded to N treatment only at seedling stage, the plr1 mutant regulated LR development under low to increasing N levels, both at seedling and higher growth stages. These results suggest that plr1 mutation gene is involved in reduced basal shoot starch accumulation and increased root sugar level for the promotion of L-type LR development, and thus would be very useful in improving rice root architecture.

Published
2022

19. Enhanced Root System Development Responses of a Newly Identified Mutation Gene Promoting Lateral Root Development to Various Nitrogen Conditions in Rice

Author

Lucob-Agustin, Nonawin, Hasegawa, Tomomi, Jinno, Kyosuke, Suralta, Roel R., Niones, Jonathan M., Kano-Nakata, Mana, Yamauchi, Akira, and Inukai, Yoshiaki

Subjects
root system architecture, rice, mutant, lateral root, nitrogen
Abstract

Lateral roots (LRs), which largely constitute the root system, allow the entire root system to expand to a larger area to efficiently capture water and nutrients from the soil. Thus, the optimization of LRs should be considered for the genetic improvement of root system architecture to most notably impact plant acquisition of soil resources for productivity. In this study, we newly identified a rice mutant, 11NB10, which has a high number of thick, long, and highly branched LRs (L-type LRs) with promoted parental root growth. We evaluated the root performance of this mutant under various nitrogen (N) regimes, including 30, 60, and 120 mg N corresponding to low, standard, and high N conditions, respectively. The results showed that under low N conditions, the 11NB10 mutant had a larger root system based on its total root length, which increased further with increasing N levels, compared to its wild-type, Nipponbare. This promoted root system growth could be attributed to the development of highly branched L-type LRs, which in turn might contributed to higher leaf area and shoot dry matter production. These findings suggest that the 11NB10 mutation gene promotes a highly developed root system under low N conditions, and its root performance could be further improved by enhancing LR development through N application. Thus, the 11NB10 mutant is a promising line for the breeding programs targeting root system architecture in rice., This work was supported mainly by JSPS KAKENHI Grant Number 18H02174.

Published
2020

21. Usefulness of Pain Scales for Postoperative Pain in Dogs

Author

KAWAHARADA, Mika, primary, OGAWA, Mizuho, additional, HASEGAWA, Tomomi, additional, FUCHIGAMI, Hitomi, additional, TAKASHIMA, Satoshi, additional, NISHII, Naohito, additional, OZAWA, Tsuyoshi, additional, NAGAHAMA, Shotaro, additional, and SHIBATA, Sanae, additional

Published
2022
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22. The outstanding rooting1 mutation gene maintains shoot growth and grain yield through promoting root development in rice under water deficit field environments.

Author

Hasegawa, Tomomi, Wainaina, Cornelius Mbathi, Shibata, Akihide, Lucob‐Agustin, Nonawin, Makihara, Daigo, Kikuta, Mayumi, Menge, Daniel Makori, Gichuhi, Emily Waringa, Samejima, Hiroaki, Kano‐Nakata, Mana, Kimani, John Munji, Musila, Ruth Nzisa, Yamauchi, Akira, and Inukai, Yoshiaki

Subjects
*ROOT development, *GENETIC mutation, *GRAIN yields, *YIELD stress, *PADDY fields
Abstract

Drought is one of the most serious constraints to rice cultivation, even under alternate wetting and drying (AWD), which is a water‐saving management practice. In rice, enhanced root development is essential for stable shoot growth, adaptability and productivity under water deficit environments. We identified and characterized outstanding rooting1 (our1) rice mutant using hydroponics. The present study sought to examine morphological root traits of the our1 mutant and the role of the mutation gene in shoot growth and yield under AWD. Thus, we evaluated the growth performance of the our1 rice mutant in pot experiments and under field AWD conditions in Kenya. The experiments were conducted with our1 mutant, its wild type and their progenies under both AWD and continuously waterlogged (CWL) conditions. The our1 mutant possessed a well‐developed root system and exhibited particularly enhanced thin root development, which was maintained from the early vegetative stage through the reproductive stage under both pot and field AWD management. This enhanced root development promoted shoot growth through increased water uptake during rewatered conditions between drought periods in AWD. In addition, the our1 mutant showed enhanced shoot growth during the reproductive stage, resulting in the maintenance of yield under AWD fields. Genotypes harbouring our1 mutation gene showed higher yields compared to wild‐type genotypes which was attributed to their higher photosynthetic ability as a result of enhanced root activity. These results suggest the important role of a well‐developed root system architecture and enhanced root function in stabilizing rice yields under water‐limited environments. Our findings indicate that the our1 mutation gene can serve as a novel breeding material to mitigate the impact of transient drought stress on yield under AWD. [ABSTRACT FROM AUTHOR]

Published
2022
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24. Evaluation of a Newly Identified Mutation Gene that Promotes Root Elongation for Improvement of Drought Avoidance in Rice

Author

Hasegawa, Tomomi, Shibata, Akihide, Wainaina, Cornelius Mbathi, Makihara, Daigo, Samejima, Hiroaki, Kikuta, Mayumi, Menge, Daniel Makori, Kimani, John Munji, Yamauchi, Akira, and Inukai, Yoshiaki

Subjects
rice, parasitic diseases, fungi, water saving technology, food and beverages, root system, drought, Kenya
Abstract

Drought is one of the most serious abiotic stresses for rice cultivation, even under a water saving production system such as the Alternate Wetting and Drying (AWD) system and upland irrigated fields. In this study, we evaluated the performance of larger root systems in a KM07 mutant under such water management conditions. This mutant line was utilized for breeding and development of new rice germplasm adaptable to drought stress conditions in Mwea, Kenya, by crossing it with the recurrent parent of New Rice for Africa (NERICA), WAB56-104. Three mutant-type F6 lines grown under AWD and upland irrigated conditions showed different responses to drought stress. The mutant type’s greater root growth was observed in lines 2 and 3 under AWD and upland irrigation conditions, respectively. These results suggest that the highly developed root system derived from the KM07 mutant can avoid drought stress under field conditions in Kenya, and it should be utilized as breeding material for drought stress avoidance, along with water management technology.

Published
2019

30. The outstanding rooting1 mutation gene maintains shoot growth and grain yield through promoting root development in rice under water deficit field environments

Author

Hasegawa, Tomomi, primary, Wainaina, Cornelius Mbathi, additional, Shibata, Akihide, additional, Lucob‐Agustin, Nonawin, additional, Makihara, Daigo, additional, Kikuta, Mayumi, additional, Menge, Daniel Makori, additional, Gichuhi, Emily Waringa, additional, Samejima, Hiroaki, additional, Kano‐Nakata, Mana, additional, Kimani, John Munji, additional, Musila, Ruth Nzisa, additional, Yamauchi, Akira, additional, and Inukai, Yoshiaki, additional

Published
2021
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34. Suppression of nitrosative and oxidative stress to reduce cardiac allograft vasculopathy

Author

Hasegawa, Tomomi, Iwanaga, Koichiro, Hultquist, Donald E., Liao, Hui, Visovatti, Scott H., and Pinsky, David J.

Subjects
Antioxidants -- Health aspects, Myocardial ischemia -- Genetic aspects, Myocardial ischemia -- Care and treatment, Myocardial ischemia -- Research, Oxidative stress -- Risk factors, Oxidative stress -- Physiological aspects, Oxidative stress -- Control, Oxidative stress -- Research, Heart -- Transplantation, Heart -- Health aspects, Biological sciences
Abstract

Oxidant injury occurs when an organ is severed from its native blood supply and then reperfused and continues during subsequent periods of immune attack. Experiments here test the hypothesis that an antioxidant given only in the peri-reperfusion period protects against not only oxidative but also nitrosative stress, leading to reduced vasculopathy long after cardiac allotranSp1antation. Experiments were performed using a murine heterotopic cardiac tranSp1antation model. An antioxidant, in the form of intraperitoneal high-dose riboflavin, was given to recipients during the initial 3 days after tranSp1antation. Antioxidant-treated mice showed significantly longer graft survival than control mice. At 4 h after tranSp1antation, antioxidant treatment significantly reduced graft lipid peroxidation and oxidized DNA and preserved antioxidant enzyme activity. At day 6 posttranSp1antation, the redox-sensitive transcription factor nuclear factor-[kappa]B and inducible nitric oxide synthase were significantly reduced following antioxidant treatment, with concomitant reduction of nitrotyrosine. Despite the limited duration of antioxidant treatment, both acute and chronic rejection were significantly suppressed. In vitro experiments confirmed suppression of nitrosative and oxidative stress and cardiomyocyte damage in antioxidant-treated cardiac allografts. Collectively, antioxidant administration during the initial 3 days after tranSp1antation significantly reduces nitrosative and oxidative stress in cardiac allografts, modulates immune responses, and protects against vasculopathy. antioxidant; ischemia-repeffusion injury; inducible nitric oxide synthase; nuclear factor-[kappa]B; riboflavin

Published
2009

35. Mutation of OUR1/OsbZIP1, which encodes a member of the basic leucine zipper transcription factor family, promotes root development in rice through repressing auxin signaling

Author

Hasegawa, Tomomi, primary, Lucob-Agustin, Nonawin, additional, Yasufuku, Koki, additional, Kojima, Takaaki, additional, Nishiuchi, Shunsaku, additional, Ogawa, Atsushi, additional, Takahashi-Nosaka, Misuzu, additional, Kano-Nakata, Mana, additional, Inari-Ikeda, Mayuko, additional, Sato, Moeko, additional, Tsuji, Hiroyuki, additional, Wainaina, Cornelius Mbathi, additional, Yamauchi, Akira, additional, and Inukai, Yoshiaki, additional

Published
2021
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39. Inhibitory effect of traditional herbal (kampo) medicines on the replication of human parainfluenza virus type 2 in vitro

Author

Uematsu, Jun, primary, Yamamoto, Hidetaka, additional, Kihira, Sahoko, additional, Sakai-Sugino, Kae, additional, Ishiyama, Yuhko, additional, Chindoh, Misaki, additional, Baba, Aya, additional, Kazuta, Rina, additional, Hasegawa, Tomomi, additional, Fujimoto, Keiko, additional, Funauchi, Aya, additional, Itoh, Ai, additional, Ookohchi, Ayumi, additional, Satoh, Saemi, additional, Maeda, Yurie, additional, Kawano, Mitsuo, additional, Tsurudome, Masato, additional, Nishio, Miwako, additional, Hirai, Kazuyuki, additional, O’Brien, Myles, additional, and Komada, Hiroshi, additional

Published
2021
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43. The promoted lateral root 1 (plr1) mutation is involved in reduced basal shoot starch accumulation and increased root sugars for enhanced lateral root growth in rice

Author

Lucob-Agustin, Nonawin, primary, Sugiura, Daisuke, additional, Kano-Nakata, Mana, additional, Hasegawa, Tomomi, additional, Suralta, Roel R., additional, Niones, Jonathan M., additional, Inari-Ikeda, Mayuko, additional, Yamauchi, Akira, additional, and Inukai, Yoshiaki, additional

Published
2020
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