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A simple low-cost approach at various exposure times was utilized to generate cold plasma in the aim to fabricate AuNPs. UV-Visible spectra and X-ray diffraction were used to characterize the nanoparticles (XRD). Surface Plasmon resonance was observed in the synthesized AuNPs at 530, 540, and 533 nm. For all samples, the patterns of XRD show very intensive peaks implying the fcc crystalline structure of AuNPs. The average crystallite size of AuNPs is ranging between 20-30 nm. The observation of morphology by FESEM revealed the spherical formation of AuNPs. Doses of 100 and 200 ppm of AuNPs were adapted to investigate their effect on the blood-mixture with and without a 20-second of cold plasma exposure. The WBC components in the blood rose as the AuNPs doses increased, whereas, the amount of (pt) in the blood fell down throughout the two weeks of AuNPs doses for the groups which exposed to AuNPs only, the level of (pt) in the blood increased in the groups which are exposed to AuNPs combined with cold plasma. While the RBC unaffected. [ABSTRACT FROM AUTHOR]