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Your search keyword '"Haruko Iki"' showing total 5 results
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5 results on '"Haruko Iki"'

1. Diversity of Chlamydiales detected in pet birds privately kept in individual homes in Japan.

Author

Yukiko SASSA-O'BRIEN, Kenji OHYA, Shunsuke IKEUCHI, Naoko SANADA, Yasuyuki SANADA, Atsushi KOJIMA, YORISAKI, Mario, Kazumasa EBISAWA, Kengo UEDA, Haruko IKI, Masaya MIZUKAMI, Hideki HAYASHIDANI, and Hideto FUKUSHI

Subjects
ABORTION in animals, BIRDS, POLYMERASE chain reaction, KLEBSIELLA pneumoniae, NUCLEIC acids
Abstract

Chlamydia-related bacteria of the Chlamydiales order have recently been described as emerging pathogens that cause pneumonia and abortion in animals and humans. We investigated the presence of Chlamydiales using real-time polymerase chain reaction (PCR) by targeting the 16S rRNA gene of a broad range of Chlamydiales in 827 fecal samples from pet birds kept in individual homes in Japan. Of the 827 samples, 493 (59.6%) tested positive for the Chlamydiales 16S rRNA gene in the real-time PCR assay. We determined the nucleic acid sequences of PCR products from 17 Chlamydiales strains. A homology search and phylogenetic analysis using these sequences confirmed that the detected Chlamydiales included C. pecorum and a broad range of Chlamydiarelated bacteria. To the best of our knowledge, this is the first study to detect a wide range of Chlamydia-related bacteria in birds. [ABSTRACT FROM AUTHOR]

Published
2023
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2. Isolation of avian bornaviruses from psittacine birds using QT6 quail cells in Japan

Author

Keizo Tomonaga, Yukiko Sassa, Tokuma Yanai, Kazumasa Ebisawa, Hideto Fukushi, Masayuki Horie, and Haruko Iki

Subjects
0301 basic medicine, Pathology, medicine.medical_specialty, Virus Cultivation, animal structures, Coturnix, Psittaciformes, Cell Line, 03 medical and health sciences, Psittaciform 1 bornavirus, Proventricular dilatation disease, Japan, Virology, biology.animal, Bornaviruses, medicine, Animals, Avian bornavirus, General Veterinary, biology, Note, Pathogenicity, biology.organism_classification, Isolation (microbiology), Quail, parrot bornavirus, QT6 cells, 030104 developmental biology, Bornaviridae, avian bornavirus, isolation
Abstract

Avian bornaviruses (ABVs) were recently discovered as the causative agents of proventricular dilatation disease (PDD). Although molecular epidemiological studies revealed that ABVs exist in Japan, no Japanese isolate has been reported thus far. In this study, we isolated four strains of Psittaciform 1 bornavirus from psittacine birds affected by PDD using QT6 quail cells. To our knowledge, this is the first report to isolate ABVs in Japan and to show that QT6 cells are available for ABV isolation. These isolates and QT6 cells would be powerful tools for elucidating the fundamental biology and pathogenicity of ABVs.

Published
2016

3. Molecular epidemiology of avian bornavirus from pet birds in Japan

Author

Keizo Tomonaga, Masaya Mizugami, Tetsuya Mizutani, Tetsuya Furuya, Masayuki Horie, Yukiko Sassa, Naomi Nishiura, Makoto Nagai, Kazumasa Ebisawa, Haruko Iki, Tsutomu Omatsu, Atsushi Kojima, Kan Fujino, Sachiko Okazaki, and Kengo Ueda

Subjects
animal structures, Genotype, Molecular Sequence Data, Autophagia, Prevalence, Zoology, Proventricular dilatation disease, Japan, Virology, Genetics, medicine, Animals, Avian bornavirus, Molecular Biology, Phylogeny, Molecular Epidemiology, Molecular epidemiology, biology, Bird Diseases, Mononegavirales Infections, General Medicine, Anatomy, Pets, medicine.disease, Pathogenicity, biology.organism_classification, Feather, visual_art, Bornaviridae, visual_art.visual_art_medium, Cloaca
Abstract

Recently, Avian bornavirus (ABV) was detected in proventricular dilatation disease (PDD) affected-birds and feather picking diseases affected-birds. However, the pathogenicity of ABV has not been thoroughly investigated. In this study, we surveyed ABV in pet birds in Japan. We found four ABV-infected birds among 93 pet birds using RT-PCR, and genotypes of the ABV were determined as ABV-2 and -4. Two of the birds positive for ABV-4 showed proventricular dilatation typically found in PDD, and chronic stomach disturbance, whereas two of the birds positive for ABV-2 showed unexplained behavioral problems that are tapping, autophagia, and cloaca prolapse.

Published
2012

4. Isolation of avian bornaviruses from psittacine birds using QT6 quail cells in Japan.

Author

Masayuki Horie, Yukiko Sassa, Haruko Iki, Kazumasa Ebisawa, Hideto Fukushi, Tokuma Yanai, and Keizo Tomonaga

Subjects
BORNA disease virus, PROVENTRICULAR dilatation syndrome, VIRUS isolation
Abstract

Avian bornaviruses (ABVs) were recently discovered as the causative agents of proventricular dilatation disease (PDD). Although molecular epidemiological studies revealed that ABVs exist in Japan, no Japanese isolate has been reported thus far. In this study, we isolated four strains of Psittaciform 1 bornavirus from psittacine birds affected by PDD using QT6 quail cells. To our knowledge, this is the first report to isolate ABVs in Japan and to show that QT6 cells are available for ABV isolation. These isolates and QT6 cells would be powerful tools for elucidating the fundamental biology and pathogenicity of ABVs. [ABSTRACT FROM AUTHOR]

Published
2016
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5. Comparative studies on distribution and fine morphology of the intestinal Peyer's patches in Mongolian gerbils (Meriones unguiculatus) and mice

Author

Shigeki Tsutsui, Haruko Iki, Mio Ohmura, Kosaku Fujiwara, and Satoshi Komazawa

Subjects
Pathology, medicine.medical_specialty, Morphology (linguistics), animal diseases, Crypt, Ileum, Biology, digestive system, law.invention, Mice, Peyer's Patches, law, parasitic diseases, Intestine, Small, medicine, Distribution (pharmacology), Animals, Microfold cell, Mice, Inbred ICR, General Veterinary, Microvilli, Peyer's patch, medicine.anatomical_structure, Duodenum, Microscopy, Electron, Scanning, Female, sense organs, Electron microscope, Gerbillinae
Abstract

The intestinal Peyer's patches (PP) were significantly different in distribution and fine morphology between Mongolian gerbils and mice. The PP of gerbils were evenly distributed from the duodenum to ileum, whereas the PP of mice were more densely distributed in the lower ileum than other parts of the intestine. In gerbils, each PP had a much greater number of lymphoid follicles than in mice, although the total number of PP was smaller. Electron microscopy revealed that the PP dome of gerbils was covered with two types of epithelial cells, one with shorter microvilli and the other with longer ones, whereas the epithelial cells of the murine PP dome was uniform-shaped with numerous medium-sized microvilli. Some dome absorptive cells of gerbils were morphologically similar to poorly differentiated crypt cells of mice, suggesting to be immature M cells.

Published
1991

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