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198 results on '"Harthoorn, Lucien F."'

4. A specific synbiotic-containing amino acid-based formula restores gut microbiota in non-IgE mediated cow’s milk allergic infants: a randomized controlled trial

Author

Wopereis, Harm, van Ampting, Marleen T. J., Cetinyurek-Yavuz, Aysun, Slump, Rob, Candy, David C. A., Butt, Assad M., Peroni, Diego G., Vandenplas, Yvan, Fox, Adam T., Shah, Neil, Roeselers, Guus, Harthoorn, Lucien F., Michaelis, Louise J., Knol, Jan, West, Christina E., and the ASSIGN study group

Published
2019
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5. Assessment of infant outgrowth of cow's milk allergy in relation to the faecal microbiome and metaproteome.

Author

Hendrickx, Diana M., An, Ran, Boeren, Sjef, Mutte, Sumanth K., PRESTO study team, Chatchatee, Pantipa, Nowak-Wegrzyn, Anna, Lange, Lars, Benjaponpitak, Suwat, Chong, Kok Wee, Sangsupawanich, Pasuree, van Ampting, Marleen T. J., Oude Nijhuis, Manon M., Harthoorn, Lucien F., Langford, Jane E., Knol, Jan, Knipping, Karen, Garssen, Johan, Trendelenburg, Valerie, and Pesek, Robert

Subjects
MILK allergy, INFANTS, GUT microbiome, FOOD allergy
Abstract

Previous studies provide evidence for an association between modifications of the gut microbiota in early life and the development of food allergies. We studied the faecal microbiota composition (16S rRNA gene amplicon sequencing) and faecal microbiome functionality (metaproteomics) in a cohort of 40 infants diagnosed with cow's milk allergy (CMA) when entering the study. Some of the infants showed outgrowth of CMA after 12 months, while others did not. Faecal microbiota composition of infants was analysed directly after CMA diagnosis (baseline) as well as 6 and 12 months after entering the study. The aim was to gain insight on gut microbiome parameters in relation to outgrowth of CMA. The results of this study show that microbiome differences related to outgrowth of CMA can be mainly identified at the taxonomic level of the 16S rRNA gene, and to a lesser extent at the protein-based microbial taxonomy and functional protein level. At the 16S rRNA gene level outgrowth of CMA is characterized by lower relative abundance of Lachnospiraceae at baseline and lower Bacteroidaceae at visit 12 months. [ABSTRACT FROM AUTHOR]

Published
2023
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6. Tolerance development in cow's milk–allergic infants receiving amino acid–based formula : A randomized controlled trial

Author

Chatchatee, Pantipa, Nowak-Wegrzyn, Anna, Lange, Lars, Benjaponpitak, Suwat, Chong, Kok Wee, Sangsupawanich, Pasuree, van Ampting, Marleen T.J., Oude Nijhuis, Manon M., Harthoorn, Lucien F., Langford, Jane E., Knol, Jan, Knipping, Karen, Garssen, Johan, Trendelenburg, Valerie, Pesek, Robert, Davis, Carla M., Muraro, Antonella, Erlewyn-Lajeunesse, Mich, Fox, Adam T., Michaelis, Louise J., Beyer, Kirsten, Noimark, Lee, Stiefel, Gary, Schauer, Uwe, Hamelman, Peroni, Diego, Boner, Chatchatee, Pantipa, Nowak-Wegrzyn, Anna, Lange, Lars, Benjaponpitak, Suwat, Chong, Kok Wee, Sangsupawanich, Pasuree, van Ampting, Marleen T.J., Oude Nijhuis, Manon M., Harthoorn, Lucien F., Langford, Jane E., Knol, Jan, Knipping, Karen, Garssen, Johan, Trendelenburg, Valerie, Pesek, Robert, Davis, Carla M., Muraro, Antonella, Erlewyn-Lajeunesse, Mich, Fox, Adam T., Michaelis, Louise J., Beyer, Kirsten, Noimark, Lee, Stiefel, Gary, Schauer, Uwe, Hamelman, Peroni, Diego, and Boner

Abstract

Background: Tolerance development is an important clinical outcome for infants with cow's milk allergy. Objective: This multicenter, prospective, randomized, double-blind, controlled clinical study (NTR3725) evaluated tolerance development to cow's milk (CM) and safety of an amino acid–based formula (AAF) including synbiotics (AAF-S) comprising prebiotic oligosaccharides (oligofructose, inulin) and probiotic Bifidobacterium breve M-16V in infants with confirmed IgE-mediated CM allergy. Methods: Subjects aged ≤13 months with IgE-mediated CM allergy were randomized to receive AAF-S (n = 80) or AAF (n = 89) for 12 months. Stratification was based on CM skin prick test wheal size and study site. After 12 and 24 months, CM tolerance was evaluated by double-blind, placebo-controlled food challenge. A logistic regression model used the all-subjects randomized data set. Results: At baseline, mean ± SD age was 9.36 ± 2.53 months. At 12 and 24 months, respectively, 49% and 62% of subjects were CM tolerant (AAF-S 45% and 64%; AAF 52% and 59%), and not differ significantly between groups. During the 12-month intervention, the number of subjects reporting at least 1 adverse event did not significantly differ between groups; however, fewer subjects required hospitalization due to serious adverse events categorized as infections in the AAF-S versus AAF group (9% vs 20%; P = .036). Conclusions: After 12 and 24 months, CM tolerance was not different between groups and was in line with natural outgrowth. Results suggest that during the intervention, fewer subjects receiving AAF-S required hospitalization due to infections.

Published
2022

9. A free amino acid‐based diet partially prevents symptoms of cow's milk allergy in mice after oral sensitization with whey

Author

van Sadelhoff, J.H.J., Hogenkamp, A., Wiertsema, S.P., Harthoorn, Lucien F, Loonstra, Reinilde, Hartog, Anita, Garssen, J., Afd Pharmacology, Pharmacology, Afd Pharmacology, and Pharmacology

Subjects
0301 basic medicine, lcsh:Immunologic diseases. Allergy, Allergy, medicine.medical_specialty, Whey protein, Immunology, Administration, Oral, Milk allergy, free amino acids, Allergic sensitization, Mice, 03 medical and health sciences, Chymases, 0302 clinical medicine, prevention, Internal medicine, medicine, Animals, Immunology and Allergy, Mast Cells, Amino Acids, Anaphylaxis, Sensitization, Original Research, Mice, Inbred C3H, business.industry, Dietary management, infant milk formula, Allergens, Immunoglobulin E, medicine.disease, Mast cell, allergy, Disease Models, Animal, Whey Proteins, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Immunoglobulin G, Dietary Supplements, Cattle, Female, Milk Hypersensitivity, business, lcsh:RC581-607, 030215 immunology
Abstract

Background Amino acid‐based formulas (AAFs) are used for the dietary management of cow's milk allergy (CMA). Whether AAFs have the potential to prevent the development and/or symptoms of CMA is not known. Objective The present study evaluated the preventive effects of an amino acid (AA)‐based diet on allergic sensitization and symptoms of CMA in mice and aimed to provide insight into the underlying mechanism. Methods C3H/HeOuJ mice were sensitized with whey protein or with phosphate‐buffered saline as sham‐sensitized control. Starting 2 weeks before sensitization, mice were fed with either a protein‐based diet or an AA‐based diet with an AA composition based on that of the AAF Neocate, a commercially available AAF prescribed for the dietary management of CMA. Upon challenge, allergic symptoms, mast cell degranulation, whey‐specific immunoglobulin levels, and FoxP3+ cell counts in jejunum sections were assessed. Results Compared to mice fed with the protein‐based diet, AA‐fed mice had significantly lower acute allergic skin responses. Moreover, the AA‐based diet prevented the whey‐induced symptoms of anaphylaxis and drop in body temperature. Whereas the AA‐based diet had no effect on the levels of serum IgE and mucosal mast cell protease‐1 (mMCP‐1), AA‐fed mice had significantly lower serum IgG2a levels and tended to have lower IgG1 levels (P = .076). In addition, the AA‐based diet prevented the whey‐induced decrease in FoxP3+ cells. In sham‐sensitized mice, no differences between the two diets were observed in any of the tested parameters. Conclusion This study demonstrates that an AA‐based diet can at least partially prevent allergic symptoms of CMA in mice. Differences in FoxP3+ cell counts and serum levels of IgG2a and IgG1 may suggest enhanced anti‐inflammatory and tolerizing capacities in AA‐fed mice. This, combined with the absence of effects in sham‐sensitized mice indicates that AAFs for the prevention of food allergies may be an interesting concept that warrants further research., This study demonstrates that an amino acid‐based diet can at least partially prevent clinical symptoms of cow's milk allergy (CMA) in mice. Differences in FoxP3+ cell counts and serum levels of IgG2a and IgG1 suggest enhanced anti‐inflammatory and tolerizing capacities in mice fed the amino acid‐based diet.

Published
2020

18. Development of a self-limiting model of Methotrexate-induced mucositis reinforces butyrate as a potential therapy

Author

Ferreira, Ana Rita da Silva, primary, Aa, Stijn A.J. van der, additional, Wehkamp, Tjalling, additional, Wardill, Hannah R., additional, Klooster, Jean Paul Ten, additional, Garssen, Johan, additional, Harthoorn, Lucien F., additional, Hartog, Anita, additional, Harmsen, Hermie J.M., additional, Tissing, Wim J.E., additional, and Bergenhenegouwen, Jeroen van, additional

Published
2020
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21. Gut microbiota from infant with cow's milk allergy promotes clinical and immune features of atopy in a murine model

Author

Afd Pharmacology, Pharmacology, Mauras, Aurélie, Wopereis, Harm, Yeop, Intan, Esber, Nathalie, Delannoy, Johanne, Labellie, Chantal, Reygner, Julie, Kapel, Nathalie, Slump, Rob, van Eijndthoven, Tiemen, Rutten, Lieke, Knol, Jan, Garssen, Johan, Harthoorn, Lucien F, Butel, Marie-José, Bajaj-Elliott, Mona, Hartog, Anita, Waligora-Dupriet, Anne-Judith, Afd Pharmacology, Pharmacology, Mauras, Aurélie, Wopereis, Harm, Yeop, Intan, Esber, Nathalie, Delannoy, Johanne, Labellie, Chantal, Reygner, Julie, Kapel, Nathalie, Slump, Rob, van Eijndthoven, Tiemen, Rutten, Lieke, Knol, Jan, Garssen, Johan, Harthoorn, Lucien F, Butel, Marie-José, Bajaj-Elliott, Mona, Hartog, Anita, and Waligora-Dupriet, Anne-Judith

Published
2019

22. A specific synbiotic-containing amino acid-based formula in dietary management of cow's milk allergy: A randomized controlled trial

Author

Afd Pharmacology, Pharmacology, Fox, Adam T., Wopereis, Harm, Van Ampting, Marleen T.J., Oude Nijhuis, Manon M., Butt, Assad M., Peroni, Diego G., Vandenplas, Yvan, Candy, David C.A., Shah, Neil, West, Christina E., Garssen, Johan, Harthoorn, Lucien F., Knol, Jan, Michaelis, Louise J., Afd Pharmacology, Pharmacology, Fox, Adam T., Wopereis, Harm, Van Ampting, Marleen T.J., Oude Nijhuis, Manon M., Butt, Assad M., Peroni, Diego G., Vandenplas, Yvan, Candy, David C.A., Shah, Neil, West, Christina E., Garssen, Johan, Harthoorn, Lucien F., Knol, Jan, and Michaelis, Louise J.

Published
2019

24. A synbiotic-containing amino-acid-based formula improves gut microbiota in non-IgE-mediated allergic infants

Author

Candy, David C A, Van Ampting, Marleen T J, Nijhuis, Manon M Oude, Wopereis, Harm, Butt, Assad M, Peroni, Diego G, Vandenplas, Yvan, Fox, Adam T, Shah, Neil, West, Christina E, Garssen, Johan, Harthoorn, Lucien F, Knol, Jan, Michaelis, Louise J, Afd Pharmacology, Pharmacology, Afd Pharmacology, Pharmacology, Growth and Development, Clinical sciences, and Pediatrics

Subjects
0301 basic medicine, Male, Synbiotics, medicine.medical_treatment, ved/biology.organism_classification_rank.species, Milk allergy, Gut flora, Gastroenterology, Pediatrics, law.invention, Probiotic, 0302 clinical medicine, fluids and secretions, law, Microbiologie, Amino Acids, Bifidobacterium breve, biology, Pediatrik, food and beverages, Infant Formula, Milk, Treatment Outcome, Female, medicine.medical_specialty, Microbiology, Article, 03 medical and health sciences, Double-Blind Method, Internal medicine, medicine, Animals, Humans, Life Science, Pediatrics, Perinatology, and Child Health, VLAG, Clostridium, business.industry, ved/biology, Eubacterium, Prebiotic, Infant, Hypoallergenic, Immunoglobulin E, medicine.disease, biology.organism_classification, Gastrointestinal Microbiome, 030104 developmental biology, 030228 respiratory system, Infant formula, Pediatrics, Perinatology and Child Health, Cattle, Milk Hypersensitivity, business
Abstract

BackgroundPrebiotics and probiotics (synbiotics) can modify gut microbiota and have potential in allergy management when combined with amino-acid-based formula (AAF) for infants with cow's milk allergy (CMA).MethodsThis multicenter, double-blind, randomized controlled trial investigated the effects of an AAF-including synbiotic blend on percentages of bifidobacteria and Eubacterium rectale/Clostridium coccoides group (ER/CC) in feces from infants with suspected non-IgE-mediated CMA. Feces from age-matched healthy breastfed infants were used as reference (healthy breastfed reference (HBR)) for primary outcomes. The CMA subjects were randomized and received test or control formula for 8 weeks. Test formula was a hypoallergenic, nutritionally complete AAF including a prebiotic blend of fructo-oligosaccharides and the probiotic strain Bifidobacterium breve M-16V. Control formula was AAF without synbiotics.ResultsA total of 35 (test) and 36 (control) subjects were randomized; HBR included 51 infants. At week 8, the median percentage of bifidobacteria was higher in the test group than in the control group (35.4% vs. 9.7%, respectively; P

Published
2018

26. Gut microbiota from infant with cow’s milk allergy promotes clinical and immune features of atopy in a murine model

Author

Mauras, Aurélie, primary, Wopereis, Harm, additional, Yeop, Intan, additional, Esber, Nathalie, additional, Delannoy, Johanne, additional, Labellie, Chantal, additional, Reygner, Julie, additional, Kapel, Nathalie, additional, Slump, Rob, additional, Eijndthoven, Tiemen, additional, Rutten, Lieke, additional, Knol, Jan, additional, Garssen, Johan, additional, Harthoorn, Lucien F., additional, Butel, Marie‐José, additional, Bajaj‐Elliott, Mona, additional, Hartog, Anita, additional, and Waligora‐Dupriet, Anne‐Judith, additional

Published
2019
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28. A synbiotic-containing amino-acid-based formula improves gut microbiota in non-IgE-mediated allergic infants

Author

Candy, David C. A., Van Ampting, Marleen T. J., Nijhuis, Manon M. Oude, Wopereis, Harm, Butt, Assad M., Peroni, Diego G., Vandenplas, Yvan, Fox, Adam T., Shah, Neil, West, Christina E., Garssen, Johan, Harthoorn, Lucien F., Knol, Jan, Michaelis, Louise J., Candy, David C. A., Van Ampting, Marleen T. J., Nijhuis, Manon M. Oude, Wopereis, Harm, Butt, Assad M., Peroni, Diego G., Vandenplas, Yvan, Fox, Adam T., Shah, Neil, West, Christina E., Garssen, Johan, Harthoorn, Lucien F., Knol, Jan, and Michaelis, Louise J.

Abstract

Background: Prebiotics and probiotics (synbiotics) can modify gut microbiota and have potential in allergy management when combined with amino-acid-based formula (AAF) for infants with cow’s milk allergy (CMA). Methods: This multicenter, double-blind, randomized controlled trial investigated the effects of an AAF-including synbiotic blend on percentages of bifidobacteria and Eubacterium rectale/Clostridium coccoides group (ER/CC) in feces from infants with suspected non-IgE-mediated CMA. Feces from age-matched healthy breastfed infants were used as reference (healthy breastfed reference (HBR)) for primary outcomes. The CMA subjects were randomized and received test or control formula for 8 weeks. Test formula was a hypoallergenic, nutritionally complete AAF including a prebiotic blend of fructo-oligosaccharides and the probiotic strain Bifidobacterium breve M-16V. Control formula was AAF without synbiotics. Results: A total of 35 (test) and 36 (control) subjects were randomized; HBR included 51 infants. At week 8, the median percentage of bifidobacteria was higher in the test group than in the control group (35.4% vs. 9.7%, respectively; P<0.001), whereas ER/CC was lower (9.5% vs. 24.2%, respectively; P<0.001). HBR levels of bifidobacteria and ER/CC were 55% and 6.5%, respectively. Conclusion: AAF including specific synbiotics, which results in levels of bifidobacteria and ER/CC approximating levels in the HBR group, improves the fecal microbiota of infants with suspected non-IgE-mediated CMA.

Published
2018
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29. Oral exposure to the free amino acid glycine inhibits the acute allergic response in a model of cow's milk allergy in mice

Author

Afd Pharmacology, Pharmacology, van Bergenhenegouwen, Jeroen, Braber, Saskia, Loonstra, Reinilde, Buurman, Nicole, Rutten, Lieke, Knipping, Karen, Savelkoul, Paul J, Harthoorn, Lucien F, Jahnsen, Frode L, Garssen, Johan, Hartog, Anita, Afd Pharmacology, Pharmacology, van Bergenhenegouwen, Jeroen, Braber, Saskia, Loonstra, Reinilde, Buurman, Nicole, Rutten, Lieke, Knipping, Karen, Savelkoul, Paul J, Harthoorn, Lucien F, Jahnsen, Frode L, Garssen, Johan, and Hartog, Anita

Published
2018

30. Oral exposure to the free amino acid glycine inhibits the acute allergic response in a model of cow's milk allergy in mice

Author

Pharmacology, Afd Pharmacology, van Bergenhenegouwen, Jeroen, Braber, Saskia, Loonstra, Reinilde, Buurman, Nicole, Rutten, Lieke, Knipping, Karen, Savelkoul, Paul J, Harthoorn, Lucien F, Jahnsen, Frode L, Garssen, Johan, Hartog, Anita, Pharmacology, Afd Pharmacology, van Bergenhenegouwen, Jeroen, Braber, Saskia, Loonstra, Reinilde, Buurman, Nicole, Rutten, Lieke, Knipping, Karen, Savelkoul, Paul J, Harthoorn, Lucien F, Jahnsen, Frode L, Garssen, Johan, and Hartog, Anita

Published
2018

31. A synbiotic-containing amino acid-based formula improves gut microbiota in non-IgE-mediated allergic infants

Author

Afd Pharmacology, Pharmacology, Candy, David C A, Van Ampting, Marleen T J, Nijhuis, Manon M Oude, Wopereis, Harm, Butt, Assad M, Peroni, Diego G, Vandenplas, Yvan, Fox, Adam T, Shah, Neil, West, Christina E, Garssen, Johan, Harthoorn, Lucien F, Knol, Jan, Michaelis, Louise J, Afd Pharmacology, Pharmacology, Candy, David C A, Van Ampting, Marleen T J, Nijhuis, Manon M Oude, Wopereis, Harm, Butt, Assad M, Peroni, Diego G, Vandenplas, Yvan, Fox, Adam T, Shah, Neil, West, Christina E, Garssen, Johan, Harthoorn, Lucien F, Knol, Jan, and Michaelis, Louise J

Published
2018

32. A synbiotic-containing amino-acid-based formula improves gut microbiota in non-IgE-mediated allergic infants

Author

Candy, David C.A., Van Ampting, Marleen T.J., Oude Nijhuis, Manon M., Wopereis, Harm, Butt, Assad M., Peroni, Diego G., Vandenplas, Yvan, Fox, Adam T., Shah, Neil, West, Christina E., Garssen, Johan, Harthoorn, Lucien F., Knol, Jan, Michaelis, Louise J., Candy, David C.A., Van Ampting, Marleen T.J., Oude Nijhuis, Manon M., Wopereis, Harm, Butt, Assad M., Peroni, Diego G., Vandenplas, Yvan, Fox, Adam T., Shah, Neil, West, Christina E., Garssen, Johan, Harthoorn, Lucien F., Knol, Jan, and Michaelis, Louise J.

Abstract

BackgroundPrebiotics and probiotics (synbiotics) can modify gut microbiota and have potential in allergy management when combined with amino-acid-based formula (AAF) for infants with cow's milk allergy (CMA).MethodsThis multicenter, double-blind, randomized controlled trial investigated the effects of an AAF-including synbiotic blend on percentages of bifidobacteria and Eubacterium rectale/Clostridium coccoides group (ER/CC) in feces from infants with suspected non-IgE-mediated CMA. Feces from age-matched healthy breastfed infants were used as reference (healthy breastfed reference (HBR)) for primary outcomes. The CMA subjects were randomized and received test or control formula for 8 weeks. Test formula was a hypoallergenic, nutritionally complete AAF including a prebiotic blend of fructo-oligosaccharides and the probiotic strain Bifidobacterium breve M-16V. Control formula was AAF without synbiotics.ResultsA total of 35 (test) and 36 (control) subjects were randomized; HBR included 51 infants. At week 8, the median percentage of bifidobacteria was higher in the test group than in the control group (35.4% vs. 9.7%, respectively; P<0.001), whereas ER/CC was lower (9.5% vs. 24.2%, respectively; P<0.001). HBR levels of bifidobacteria and ER/CC were 55% and 6.5%, respectively.ConclusionAAF including specific synbiotics, which results in levels of bifidobacteria and ER/CC approximating levels in the HBR group, improves the fecal microbiota of infants with suspected non-IgE-mediated CMA.

Published
2018

33. Mineral Intake and Status of Cowʼs Milk Allergic Infants Consuming an Amino Acid-based Formula

Author

Eussen, Simone R.B.M., Harvey, Bryan M., Harthoorn, Lucien F., and Burks, A. Wesley

Abstract

Data on the mineral status of infants with cow's milk allergy (CMA) consuming an amino acid-based formula (AAF) have not been published. The present study aims to assess mineral status of term infants age 0 to 8 months diagnosed with CMA receiving an AAF for 16 weeks. Serum concentrations of calcium, phosphorus, chloride, sodium, potassium, magnesium, and ferritin were determined in 82 subjects at baseline and in 66 subjects after 16 weeks on AAF using standard methods and evaluated against age-specific reference ranges. In addition to this, individual estimated energy and mineral intakes were compared to Adequate Intakes defined by the European Food Safety Authority and the US Institute of Medicine. The results of this study show that the AAF was effective in providing an adequate mineral status in infants with CMA. The vast majority of infants aged 0 to 6 months (formula only) and aged 6 to 12 months (formula and complementary foods) had adequate mineral intakes.

Published
2017
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34. Abstracts from the Food Allergy and Anaphylaxis Meeting 2016

Author

Pouessel, Guillaume, Claverie, Claire, Labreuche, Julien, Renaudin, Jean-Marie, Dorkenoo, Aimée, Eb, Mireille, Moneret-Vautrin, Anne, Deschildre, Antoine, Leteurtre, Stephane, Grabenhenrich, Linus, Worm, Margitta, Dölle, Sabine, Scherer, Kathrin, Hutteger, Isidor, Christensen, Morten, Bindslev-Jensen, Carsten, Mortz, Charlotte, Eller, Esben, Kjaer, Henrik F, Carneiro-Leão, Leonor, Badas, Jenny, Coimbra, Alice, Levy, Dikla P, Ben-Shoshan, Moshe, Rimon, Ayelet, Benor, Shira, Arends, Nicolette J T, Edelbroek, Nikki, de Groot, Hans, Emons, Joyce A M, Brand, H. K A, Verhoeven, Dirk, van Veen, Leonieke N, de Jong, Nicolette W, Noh, Geunwoong, Jang, Eun H, Pascal, Mariona, Dominguez, Olga, Piquer, Mònica, Alvaro, Montserrat, Jimenez-Feijoo, Rosa, Lozano, Jaime, Machinena, Adriana, del Mar Folqué, Maria, Giner, Maria T, Plaza, Ana M, Turner, Paul, Patel, Nandinee, Vazquez-Ortiz, Marta, Lindsley, Sarah, Walker, Lucy, Rosenberg, Simon, Mari, Adriano, Alessandri, Claudia, Giangrieco, Ivana, Tuppo, Lisa, Rafaiani, Chiara, Mitterer, Georg, Ciancamerla, Michela, Ferrara, Rosetta, Bernardi, Maria L, Zennaro, Danila, Tamburrini, Maurizio, Ciardiello, Maria A, Harwanegg, Christian, Fernandez, Antonio, Selb, Regina, Egenmann, Philippe, Epstein, Michelle, Hoffmann-Sommergruber, Karin, Koning, Frits, Lovik, Martinus, Clare Mills, E. N, Moreno, Javier, van Loveren, Henk, Wal, Jean-Michel, Diesner, Susanne, Bergmayr, Cornelia, Pfitzner, Barbara, Assmann, Vera E, Starkl, Philipp, Endesfelder, David, Eiwegger, Thomas, Szepfalusi, Zsolt, Fehrenbach, Heinz, Jensen-Jarolim, Erika, Hartmann, Anton, Pali-Schöll, Isabella, Untersmayr, Eva, Wille, Soren, Meyer, Peter, Klingebiel, Caroline, Lidholm, Jonas, Ehrenberg, Angelica, Östling, Jonas, Cleach, Isabelle, Mège, Jean-Louis, Vitte, Joana, Aina, Roberta, Dubiela, Pawel, Pfeifer, Sabine, Bublin, Merima, Radauer, Christian, Humeniuk, Piotr, Kabasser, Stefan, Asero, Riccardo, Bogas, Gador, Gomez, Francisca, Campo, Paloma, Salas, Maria, Doña, Inmaculada, Barrionuevo, Esther, Guerrero, Maria A, Mayorga, Cristobalina, Prieto, Ana, Barber, Domingo, Torres, Maria J, Jamin, Annette, Wangorsch, Andrea, Ballmer, Barbara, Vieths, Stefan, Scheurer, Stephan, Apostolovic, Danijela, Mihailovic, Jelena, Krstic, Maja, Starkhammar, Maria, Velickovic, Tanja C, Hamsten, Carl, van Hage, Marianne, van Erp, Francine C, Knol, Edward F, Kansen, Hannah M, Pontoppidan, Bo, Meijer, Yolanda, van der Ent, Cornelis K, Knulst, André C, Sayers, Rebekah, Brown, Helen, Custovic, Adnan, Simpson, Angela, Mills, Claire, Schulz, Juliane, Akkerdaas, Jaap, Totis, Muriel, Capt, Annabelle, Herouet-Guicheney, Corinne, van Ree, Ronald, Banerjee, Tushar, Banerjee, Antima, Claude, Mathilde, Bouchaud, Grégory, Lupi, Roberta, Castan, Laure, Tranquet, Olivier, Denery-Papini, Sandra, Bodinier, Marie, Brossard, Chantal, De Poi, Rosella, Gritti, Elisa, De Dominicis, Emiliano, Popping, Bert, de Laureto, Patrizia P, Palosuo, Kati, Kukkonen, Anna K, Pelkonen, Anna, Mäkelä, Mika, Lee, Nanju A, Rost, Johanna, Muralidharan, Sridevi, Campbell, Dianne, Mehr, Sam, Nock, Catherine, Baumert, Joseph, Taylor, Steve, Mastrorilli, Carla, Tripodi, Salvatore, Caffarelli, Carlo, Perna, Serena, Di Rienzo Businco, Andrea, Sfika, Ifigenia, Dondi, Arianna, Bianchi, Annamaria, Dascola, Carlotta P, Ricci, Giampaolo, Cipriani, Francesca, Maiello, Nunzia, del Giudice, Michele M, Frediani, Tullio, Frediani, Simone, Macrì, Francesco, Pistoletti, Chiara, Iacono, Iride D, Patria, Maria F, Varin, Elena, Peroni, Diego, Comberiati, Pasquale, Chini, Loredana, Moschese, Viviana, Lucarelli, Sandra, Bernardini, Roberto, Pingitore, Giuseppe, Pelosi, Umberto, Olcese, Roberta, Moretti, Matteo, Cirisano, Anastasia, Faggian, Diego, Travaglini, Alessandro, Plebani, Mario, Verga, Maria C, Calvani, Mauro, Giordani, Paolo, Matricardi, Paolo M, Ontiveros, Noe, Cabrera-Chavez, Francisco, Galand, Julie, Beaudouin, Etienne, Pineau, Florence, Sakai, Shinobu, Matsunaga, Kayoko, Teshima, Reiko, Larré, Colette, Denery, Sandra, Tschirner, Sebastian, Trendelenburg, Valérie, Schulz, Gabriele, Niggemann, Bodo, Beyer, Kirsten, Bouferkas, Youcef, Belabbas, Younes, Saidi, Djamel, Kheroua, Omar, Mecherfi, Kamel E E, Guendouz, Malika, Haddi, Abir, Kaddouri, Hanane, Amaral, Luis, Pereira, Ana, Rodrigues, Susana, Datema, Mareen, 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Cipriani, Francesca, Maiello, Nunzia, del Giudice, Michele M, Frediani, Tullio, Frediani, Simone, Macrì, Francesco, Pistoletti, Chiara, Iacono, Iride D, Patria, Maria F, Varin, Elena, Peroni, Diego, Comberiati, Pasquale, Chini, Loredana, Moschese, Viviana, Lucarelli, Sandra, Bernardini, Roberto, Pingitore, Giuseppe, Pelosi, Umberto, Olcese, Roberta, Moretti, Matteo, Cirisano, Anastasia, Faggian, Diego, Travaglini, Alessandro, Plebani, Mario, Verga, Maria C, Calvani, Mauro, Giordani, Paolo, Matricardi, Paolo M, Ontiveros, Noe, Cabrera-Chavez, Francisco, Galand, Julie, Beaudouin, Etienne, Pineau, Florence, Sakai, Shinobu, Matsunaga, Kayoko, Teshima, Reiko, Larré, Colette, Denery, Sandra, Tschirner, Sebastian, Trendelenburg, Valérie, Schulz, Gabriele, Niggemann, Bodo, Beyer, Kirsten, Bouferkas, Youcef, Belabbas, Younes, Saidi, Djamel, Kheroua, Omar, Mecherfi, Kamel E E, Guendouz, Malika, Haddi, Abir, Kaddouri, Hanane, Amaral, Luis, Pereira, Ana, Rodrigues, Susana, Datema, Mareen, 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Koppelman, Gerard H, Kollen, Boudewjin J, Zubchenko, Svitlana, Kuntz, Sarah, Mérida, Pablo, Álvaro, Montserrat, Piquer, Monica, Riggioni, Carmen, Castellanos, Juan H, Jimenez, Rosa, Cap, Melanie, Drumez, Elodie, Lejeune, Stéphanie, Thumerelle, Caroline, Mordacq, Clémence, Nève, Véronique, Ricò, Sonia, Varini, Margherita, Nocerino, Rita, Cosenza, Linda, Amoroso, Antonio, Di Costanzo, Margherita, Di Scala, Carmen, Bedogni, Giorgio, Canani, Roberto B, Turner, Paul J, Poza-Guedes, Paloma, González-Pérez, Ruperto, Sánchez-Machín, Inmaculada, Matheu-Delgado, Victor, Wambre, Erik, Ballegaard, Anne-Sofie, Madsen, Charlotte, Gregersen, Juliane, Bøgh, Katrine L, Aubert, Philippe, Neunlist, Michel, Magnan, Antoine, Lozano-Ojalvo, Daniel, Pablos-Tanarro, Alba, Pérez-Rodríguez, Leticia, Molina, Elena, López-Fandiño, Rosina, Rekima, Akila, Macchiaverni, Patricia, Turfkruyer, Mathilde, Holvoet, Sebastien, Dupuis, Lénaïck, Baiz, Nour, Annesi-Maesano, Isabella, Mercenier, Annick, Nutten, Sophie, Verhasselt, Valérie, Mrakovcic-Sutic, Ines, Banac, Srdan, Sutic, Ivana, Baricev-Novakovic, Zdenka, Sutic, Ingrid, Pavisic, Valentino, Muñoz-Cano, Rosa, Jiménez-Rodríguez, Teodoríkez, Corbacho, Daniel, Roca-Ferrer, Jordi, Bartra, Joan, Bulog, Aleksandar, Micovic, Vladimir, Markiewicz, Lidia, Szymkiewicz, Agata, Szyc, Anna, Wróblewska, Barbara, Harvey, Bryan M, Harthoorn, Lucien F, Wesley Burks, A., Rentzos, Georgios, Björk, Anna-Lena B, Bengtsson, Ulf, Barber, Colin, Kalicinsky, Chrystyna, Breynaert, Christine, Coorevits, Lieve, Jansen, Cornelia, Van Hoeyveld, Erna, Verbeke, Kristin, Kochuyt, Anne-Marie, Schrijvers, Rik, Deleanu, Diana, Muntean, Adriana, Konstantakopoulou, Maria, Pasioti, Maria, Papadopoulou, Anastasia, Iliopoulou, Anna, Mikos, Nikolaos, Kompoti, Evangelia, de Castro, Eunice D, Bartalomé, Borja, Ue, Kok L, Griffiths, Elizabeth, Till, Stephen, Grimshaw, Kate, Roberts, Graham, Selby, Anna, Butiene, Indre, Larco, Jose I, Dubakiene, Ruta, Fiandor, Ana, Fiocchi, Alessandro, Papadopoulos, Nikos, Sigurdardottir, Sigurveig, Sprikkelman, Aline, Schoemaker, Anne-Fleur, Xepapadaki, Paraskevi, Keil, Thomas, Cojocariu, Zizi, Barbado, Beatriz S, Iancu, Vasti, Arroabarren, Esozia, Esarte, Marta G, Arteaga, Miren, Andrade, Mayra C, Borges, Denise, Kalil, Jorge, Bianchi, Pedro G, Agondi, Rosana C, Gupta, Rinkesh K, Sharma, Akanksha, Gupta, Kriti, Das, Mukul, Dwivedi, Premendra, Karseladze, Rusudan, Jorjoliani, Liana, Saginadze, Lali, Tskhakaia, Mariam, Basello, Katia, Piuri, Gabriele, Speciani, Attilio F, Speciani, Michela C, Camerotto, Carla, Zinno, Francesco, Pakholchuk, Olga, Nedelska, Svitlana, Pattini, Stefano, Costantino, Maria T, Peveri, Silvia, Villalta, Danilo, Savi, Eleonora, Costanzi, Andrea, Revyakina, Vera A, Kiseleva, Marina A, Kuvshinova, Elena D, Larkova, Inna A, Shekhetov, Anton A, Silva, Diana, Moreira, André, Plácido, José, van der Kleij, Hanneke, van Twuijver, Esther, Sutorius, Robbert, de Kam, Pieter-Jan, van Odijk, Jenny, Lindqvist, Helen, Lustig, Elin, Jácome, Amyra A A, Aguilar, Karla L B, Domínguez, Miguel G, Hernández, David A M, Caruso, Cristiano, Casale, Cono, Rapaccini, Gian L, Romano, Antonino, De Vitis, Italo, Cocco, Renata R, Aranda, Carolina, Mallozi, Marcia C, Motta, Jackeline F, Moraes, Lilian, Pastorino, Antonio, Rosario, Nelson, Goudouris, Ekaterini, Porto, Arnaldo, Wandalsen, Neusa F, Sarinho, Emanuel, Sano, Flavio, Solé, Dirceu, Pitsios, Constantinos, Petrodimopoulou, Maria, Papadopoulou, Ekaterini, Passioti, Maria, Kontogianni, Meropi, Adamia, Nino, Khaleva, Ekaterina, del Prado, Ana P, Du Toit, George, Krzych, Edyta, Samolinska-Zawisza, Urszula, Furmanczyk, Konrad, Tomaszewska, Aneta, Raciborski, Filip, Lipiec, Agnieszka, Samel-Kowalik, Piotr, Walkiewicz, Artur, Borowicz, Jacek, Samolinski, Boleslaw, Nano, Aimee L, Recto, Marysia, Somoza, Maria L, López, Natalia B, Alzate, Diana P, Ruano, Francisco J, Garcimartín, Maria I, Haroun, Elisa, de la Torre, Maria V, Rojas, Antonia, Onieva, Montserrat L, Canto, Gabriela, Rodrigues, Alexandra, Forno, Andreia, Cabral, António J, Gonçalves, Rute, Vorozhko, Ilya, Sentsova, Tatyana, Chernyak, Olga, Denisova, Svetlana, Ilènko, Lidia, Muhortnich, Valery, Zimmermann, Caroline, Rohrbach, Alexander, Bakhsh, Faisal R, Boudewijn, Kollen, Oomkes-Pilon, Anne-Marie, Van Ginkle, Dorien, Šilar, Mira, Jeverica, Anja, Vesel, Tina, Avčin, Tadej, Korošec, Peter, van der Valk, Johanna, Berends, Irene, Arends, Nicolette, van Maaren, Maurits, Wichers, Harry, Emons, Joyce, Dubois, Anthony, de Jong, Nicolette, Matsyura, Oksana, Besh, Lesya, Huang, Chung-Hsiung, Jan, Tong-Rong, Stiefel, Gary, Tratt, Jean, Kirk, Kerrie, Carolino, Fabricia, Arasi, Stefania, Caminiti, Lucia, Crisafulli, Giuseppe, Fiamingo, Chiara, Fresta, Jlenia, Pajno, Giovanni, Remington, Ben, Kruizinga, Astrid, Marty Blom, W., Westerhout, Joost, Bijlsma, Sabina, Baumert, Joe, Blankestijn, Mark, Otten, Henny, Klemans, Rob, Michelsen-Huisman, Anouska D, van Os-Medendorp, Harmieke, Kruizinga, Astrid G, Versluis, Astrid, van Duijn, Gert, de Zeeuw-Brouwer, H. M, Castenmiller, Jacqueline J M, Noteborn, Hub P J M, Houben, Geert F, Bravin, Kristian, Luyt, David, Javed, Bushra, Couch, Phil, Munro, Christopher, Padfield, Phil, Sperrin, Matt, Byrne, Aideen, Oosthuizen, Lizalet, Kelleher, Carina, Ward, Fiona, Brosnan, Niamh, King, Graham, Corbet, Eva, Guzmán, Josué A H, García, Montserrat B, Asensio, Oscar, Navarrete, Laura V, Larramona, Helena, Miró, Xavier D, Pyrz, Katarzyna, Austin, Moira, Boloh, Yanne, Couch, Philip, Galloway, Deirdre, Hernandez, Pilar, Hourihane, Jonathan O, Kenna, Fiona, Majkowska-Wojciechowska, Barbara, Regent, Lynne, Themisb, Marina, Schnadt, Sabine, Semic-Jusufagic, Aida, Galvin, Audrey D, Kauppila, Tiina, Kuitunen, Mikael, Kitsioulis, Nikolaos A, Douladiris, Nikolaos, Kostoudi, Sofia, Manolaraki, Ioanna, Mitsias, Dimitris, Manousakis, Emmanouil, Papadopoulos, Nikolaos G, Knibb, Rebecca, Hammond, Jennifer, Cooke, Richard, Yrjänä, Jaakko, Hanni, Anna-Maija, Vähäsarja, Päivi, Mustonen, Oona, Dunder, Teija, Kulmala, Petri, Lasa, Eva, D’Amelio, Carmen, Martínez, Sara, Joral, Alejandro, Gastaminza, Gabriel, Goikoetxea, Maria J, Candy, David C A, Van Ampting, Marleen T J, Oude Nijhuis, Manon M, Butt, Assad M, Peroni, Diego G, Fox, Adam T, Knol, Jan, Michaelis, Louise J, Padua, Ines, Padrao, Patricia, Moreira, Pedro, Barros, Renata, Sharif, Hanan, Ahmed, Manzoor, Gomaa, Nehad, Mens, Joris, Smit, Koen, Timmermans, Frans, Poredoš, Tomaž, Jeverica, Anja K, Sedmak, Marjeta, Benedik, Evgen, Accetto, Meta, Zupančič, Mirjana, Yonamine, Glauce, Soldateli, Gustavo, Aquilante, Bruna, Pastorino, Antonio C, de Moraes Beck, Cleonir L, Gushken, Andrea K, de Barros Dorna, Mayra, dos Santos, Cristiane N, Castro, Ana P M, Al-Qahtani, Abdulhadi, Arnaout, Rand, Khaliq, Agha R, Amin, Rashid, Sheikh, Farrukh, Alvarez, Jorge, Anda, Marta, Palacios, Miriam, De Prada, Montserrat, Ponce, Carmen, Balbino, Bianca, Sibilano, Riccardo, Marichal, Thomas, Gaudenzio, Nicolas, Karasuyama, Hajime, Bruhns, Pierre, Tsai, Mindy, Reber, Laurent L, Galli, Stephen J, Ferreira, Ana R, Cernadas, Josefina R, del Campo García, Aida, Fernández, Sara P, Carrera, Nerea S, Sánchez-Cruz, Fernando B, Lorenzo, José R F, Claus, Stephanie, Pföhler, Claudia, Ruëff, Franziska, Treudler, Regina, Jaume, Mercedes E, Madroñero, Agustin, Perez, Maria T G, Julia, Juan C, Plovdiv, Charlotte H, Gethings, Lee, Langridge, Jim, Adel-Patient, Karine, Bernard, Hervé, Barcievic-Jones, Ivona, Sokolova, Raditsa, Yankova, Rumyana, Ivanovska, Mariya, Murdjeva, Marianna, Popova, Tatyana, Dermendzhiev, Svetlan, Karjalainen, Martin, Lehnigk, Ulrike, Brown, Duncan, Locklear, Julie C, Locklear, Julie, Maris, Ioana, Hourihane, Jonathan, Ornelas, Cristina, Caiado, Joana, Ferreira, Manuel B, Pereira-Barbosa, Manuel, Puente, Yolanda, Daza, Juan C, Monteseirin, Francisco J, Ukleja-Sokolowska, Natalia, Gawronska-Ukleja, Ewa, Zbikowska-Gotz, Magdalena, Bartuzi, Zbigniew, Sokolowski, Lukasz, Adams, Aine, Mahon, Bernard, English, Karen, Gourdon-Dubois, Nelly, Sellam, Laetitia, Pereira, Bruno, Michaud, Elodie, Messaoudi, Khaled, Evrard, Bertrand, Fauquert, Jean-Luc, Palomares, Francisca, Gomez, Gador, Rodriguez, Maria J, Galindo, Luisa, Molina, Ana, Paparo, Lorella, Mennini, Maurizio, Aitoro, Rosita, Wawrzeńczyk, Adam, Przybyszewski, Michał, Wawrzeńczyk, Anna, Sarıcoban, Hulya E, Ugras, Meltem, Yalvac, Zerrin, Flokstra-de Blok, Bertine M J, van der Velde, J. L, Vereda, Andrea, Ippolito, Clara, Traversa, Amaranta, Adriano, Daniela, Bianchi, Daniela M, Gallina, Silvia, Decastelli, Lucia, Makatsori, Melina, Miles, Anne, Devetak, Sonja P, Devetak, Iztok, Tabet, Soraya A, Trandbohus, Jeanette F, Winther, Pernille, Malling, Hans-Jørgen, Hansen, Kirsten S, Garvey, Lene H, Wang, Chia-Chi, Cheng, Yin-Hua, Tung, Chun-Wei, Dietrich, Mariola, Marenholz, Ingo, Kalb, Birgit, Grosche, Sarah, Blümchen, Katharina, Schlags, Rupert, Price, Mareike, Rietz, Sylke, Esparza-Gordillo, Jorge, Lau, Susanne, Lee, Young-Ae, Almontasheri, Ali, Bahkali, Mohammad A, Elshorbagi, Sahar, Alfhaid, Abdullah, Altamimi, Mashary, Madbouly, Eman, Al-Dhekri, Hassan, Arnaout, Rand K, Basagaña, Maria, Miquel, Sira, Bartolomé, Borja, Brix, Bettina, Rohwer, Stefanie, Brandhoff, Sandra, Berger, Alena, Suer, Waltraud, Weimann, Alf, Bueno, Cristina, Martín-Pedraza, Laura, Abián, Sara, Segundo-Acosta, Pablo S, López-Rodríguez, Juan C, Barderas, Rodrigo, Batanero, Eva, Cuesta-Herranz, Javier, Villalba, María T, Correia, Magna, Benito-Garcia, Filipe, Arêde, Cristina, Piedade, Susana, Morais-Almeida, Mário, Hindley, James, Yarham, Ross, Kuklinska-Pijanka, Anna, Gillick, David, Patient, Karine, Chapman, Martin D, Miranda, Ana, Matos, Eugénia, Sokolova, Anna, Rao, Huan, Baricevic-Jones, Ivona, Smith, Frances, Xue, Wentong, Magnusdottir, Helga, Vidarsdottir, Anna G, Lund, Sigrun, Jensen, Anders B, Ludviksson, Bjorn R, Simon, Reyna, Elfont, Robert, Bennett, Sean, Voyksner, Robert, de Lurdes Torre, Maria, Yürek, Songül, Faber, Margaretha A, Bastiaensen, Annick, Mangodt, Evelyne, van Gasse, Athina, Decuyper, Ine, Sabato, Vito, Hagendorens, Margo M, Bridts, Chris H, De Clerck, Luc S, Ebo, Didier, Schwarz, Susanne, Ziegert, Mandy, Albroscheit, Saskia, Schwager, Christian, Kull, Skadi, Behrends, Jochen, Röckendorf, Niels, Schocker, Frauke, Frey, Andreas, Homann, Arne, Becker, Wolf-Meinhard, Jappe, Uta, Zaabat, Nesrine, Osscini, Sylvia, Agabriel, Chantal, Sterling, Benoît, Carsin, Ania, Liabeuf, Valérie, Maćków, Monica, Zbróg, Alina, Bronkowska, Monica, Courtois, Justine, Gadisseur, Romy, Bertholet, Catherine, Lukas, Pierre, Cavalier, Etienne, Delahaut, Philippe, Quinting, Birgit, Gertmo, Margareta B, Hasseus, Ewa T, Barzylovych, Vladyslava, Oliveira, Júlio, Ensina, Luis F, Aranda, Carolina S, Dopazo, Leire, Lopez, Rebeca, Perez, Raquel, Santos-Diez, Laura, Bilbao, Agurtzane, Garcia, Juan M, Núñez, Ignacio G, Mármol, María Á A, Villarejo, María J B, Martos, José A B, Vergara, Marina S, García, José M I, Michalska, Agata, Sergiejko, Grzegorz, Zacniewski, Robert, Ghiordanescu, Ileana-Maria, Deaconu, Cristina, Popescu, Mihaela, Bumbacea, Roxana S, Ibranji, Alkerta, Nikolla, Elida, Loloci, Gjustina, Juel-Berg, Nanna, Larsen, Lau F, Poulsen, Lars K, Marcelino, João, Prata, Ricardo, Costa, Ana C, Duarte, Fátima, Neto, Marta, Santos, Jennifer, Pestana, Luís C, Sampaio, Daniel, Minale, Paola, Dignetti, Paola, Bignardi, Donatella, Nedelea, Irena, Popescu, Florin-Dan, Vieru, Mariana, Secureanu, Florin-Adrian, Ganea, Carmen S, Vieira, Miguel, Silva, José P M, Watts, Timothy, Watts, Sophia, Lomikovska, Marta, Peredelskaya, Marina, Nenasheva, Natalia, Filipovic, Ivana, Zivkovic, Zorica, Filipovic, Djordje, Higgs, Jennette, Warner, Amena, and Jones, Carla

Published
2017

35. Post-sensitization administration of non-digestible oligosaccharides and Bifidobacterium breve M-16V reduces allergic symptoms in mice

Author

van Esch, Betty C A M, Abbring, Suzanne, Diks, Mara A P, Dingjan, Gemma M, Harthoorn, Lucien F, Vos, A Paul, Garssen, Johan, and Pharmacology

Subjects
inulin, propionic acid, FOS, immunoglobulins, non-digestible oligosaccharides, cow's milk allergy, regulatory T-cells, galectin-9, probiotics, Th1-cells, fructo-oligosaccharide, acute allergic skin response, synbiotics, Bifidobacterium breve M-16V, mast cell, prebiotics, T cell subsets, short chain fatty acids
Abstract

To support dietary management of severe cow's milk allergic infants, a synbiotic mixture of non-digestible oligosaccharides and Bifidobacterium breve M-16V (B. breve) was designed from source materials that are completely cow's milk-free. It was investigated whether this specific synbiotic concept can reduce an established food allergic response in a research model for hen's egg allergy. Mice were orally sensitized once a week for 5 weeks to ovalbumin (OVA) using cholera toxin (CT) as an adjuvant. Non-sensitized mice received CT in PBS only. Sensitized mice were fed a control diet or a diet enriched with short-chain- (scFOS) and long-chain fructo-oligosaccharides (lcFOS), B. breve or scFOSlcFOS + B. breve for 3 weeks starting after the last sensitization. Non-sensitized mice received the control diet. Anaphylactic shock symptoms, acute allergic skin responses and serum specific IgE, mMCP-1 and galectin-9 were measured upon OVA challenge. Activated Th2-, Th1-cells and regulatory T-cells were quantified in spleen and mesenteric lymph nodes (MLN) and cytokine profiles were analyzed. Short chain fatty acids (SCFA) were measured in ceacal samples. The acute allergic skin response was reduced in mice fed the scFOSlcFOS + B. breve diet compared to mice fed any of the other diets. A reduction in mast cell degranulation (mMCP-1) and anaphylactic shock symptoms was also observed in these mice. Unstimulated splenocyte cultures produced increased levels of IL10 and IFNg in mice fed the scFOSlcFOS + B. breve diet. Correspondingly, increased percentages of activated Th1 cells were observed in the spleen. Allergen-specific re-stimulation of splenocytes showed a decrease in IL5 production. In summary; post-sensitization administration of scFOSlcFOS + B. breve was effective in reducing allergic symptoms after allergen challenge. These effects coincided with changes in regulatory and effector T-cell subsets and increases in the SCFA propionic acid. These results suggest immune modulatory benefits of dietary intervention with a unique combination of scFOSlcFOS + B. breve in established food allergy. Whether these effects translate to human applications is subject for ongoing clinical studies.

Published
2016

36. Esophageal and Small Intestinal Mucosal Integrity in Eosinophilic Esophagitis and Response to an Elemental Diet

Author

Warners, Marijn J, primary, Vlieg-Boerstra, Berber J, additional, Verheij, Joanne, additional, van Hamersveld, Patricia H P, additional, van Rhijn, Bram D, additional, Van Ampting, Marleen T J, additional, Harthoorn, Lucien F, additional, de Jonge, Wouter J, additional, Smout, Andreas J P M, additional, and Bredenoord, Albert J, additional

Published
2017
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42. A potential role for regulatory T-cells in the amelioration of DSS induced colitis by dietary non-digestible polysaccharides

Author

Hartog, Anita, Belle, Fabiën N, Bastiaans, Jacqueline, de Graaff, Priscilla, Garssen, Johan, Harthoorn, Lucien F, Vos, Arjan P, Hartog, Anita, Belle, Fabiën N, Bastiaans, Jacqueline, de Graaff, Priscilla, Garssen, Johan, Harthoorn, Lucien F, and Vos, Arjan P

Abstract

Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract. The interaction between a disturbed microbial composition, the intestinal mucosal barrier and the mucosal immune system plays an important role in IBD and its chronicity. It has been indicated that due to the altered microbial composition the balance between T regulatory cells (Treg) and T helper cells (Th) 17 is disturbed, leading to an inflammatory state. The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice. This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN). Moreover, the Treg percentage in the MLN correlates with the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN, suggesting that these play a role in the observed effects. In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy. Optimizing enteral nutritional concepts with the tested fibre mix, know to modulate the gut microbiota composition, SCFA production and inflammatory status (as indicated by the present study) could possibly further improve efficacy in inducing remission.

Published
2015

43. Immunomodulatory effects of amino acid-based formulae (AAF) in gastrointestinal non-IgE mediated food allergy

Author

Sub Immunopharmacology, Pharmacology, Hartog, Anita, Jones, Hannah E., Harthoorn, Lucien F., Garssen, Johan, Stephenson, Holly, Brunner, Katja, Köglmeier, Jutta, Shah, Neil, Bajaj-Elliot, Mona, Lindley, Keith J., Sub Immunopharmacology, Pharmacology, Hartog, Anita, Jones, Hannah E., Harthoorn, Lucien F., Garssen, Johan, Stephenson, Holly, Brunner, Katja, Köglmeier, Jutta, Shah, Neil, Bajaj-Elliot, Mona, and Lindley, Keith J.

Published
2015

44. A potential role for regulatory T-cells in the amelioration of DSS induced colitis by dietary non-digestible polysaccharides

Author

Sub General Pharmacology, Sub Immunopharmacology, Afd Pharmacology, Pharmacology, Hartog, Anita, Belle, Fabiën N, Bastiaans, Jacqueline, de Graaff, Priscilla, Garssen, Johan, Harthoorn, Lucien F, Vos, Arjan P, Sub General Pharmacology, Sub Immunopharmacology, Afd Pharmacology, Pharmacology, Hartog, Anita, Belle, Fabiën N, Bastiaans, Jacqueline, de Graaff, Priscilla, Garssen, Johan, Harthoorn, Lucien F, and Vos, Arjan P

Published
2015

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