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The use of cover crops can improve the environmental sustainability of intensive cropping systems. A five-year field trial was undertaken to evaluate the effect of multi-season cover cropping on weed suppression within a no-till spring-planted maize system in the Waikato region in New Zealand. Five cover crop treatments were compared: gland clover (Trifolium glanduliferum Boiss.); faba bean (Vicia faba L); oats (Avena sativa L.); and Italian ryegrass (Lolium multiflorum Lam.), and winter fallow. The results demonstrated that in this system, herbicide treatments can be reduced to one application to terminate the winter cover crop and a single maize post-emergence application, thus reducing selection pressure for herbicide-resistance, without any loss in silage or grain production. Furthermore, a legume-based cover crop gave higher yields than the grass cover-crops and reduced the damage by the maize and grass pest cosmopolitan armyworm (Mythimna separata Walker). The build-up of crop residue over time was detrimental to the establishment of the small-seeded clover and ryegrass cover crops but good weed suppression and grain yields were achieved in the final season by replacing them respectively with the larger seeded woolly pod vetch (Vicia eriocarpa (Hausskn.) Halácsy) and a blue lupin (Lupinus angustifolius L.)/ mustard (Sinapis alba L.) mix.

Zona-free somatic cell transfer (SCT) and embryo aggregation increase throughput and efficiency of cloned embryo and offspring production, respectively, but both approaches have not been widely adopted. Cloning efficiency is further improved by cell cycle coordination between the interphase donor cell and metaphase-arrested recipient cytoplast. This commonly involves inclusion of caffeine and omission of calcium to maintain high mitotic cyclin-dependent kinase activity and low calcium levels, respectively, in the nonactivated cytoplast. The aim of our study was to integrate these various methodological improvements into a single work stream that increases sheep cloning success. We show that omitting calcium during zona-free SCT improved blastocyst development from 6% to 13%, while caffeine treatment reduced spontaneous oocyte activation from 17% to 8%. In a retrospective analysis, morula aggregation produced high morphological quality blastocysts with better