1. Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development.
- Author
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Li H, Watson A, Olechwier A, Anaya M, Sorooshyari SK, Harnett DP, Lee HP, Vielmetter J, Fares MA, Garcia KC, Özkan E, Labrador JP, and Zinn K
- Subjects
- Animals, Antibodies chemistry, Biological Assay, Computational Biology, Drosophila Proteins metabolism, Drosophila melanogaster genetics, Drosophila melanogaster growth & development, Drosophila melanogaster ultrastructure, Embryo, Nonmammalian, Fluorescent Dyes chemistry, Gene Expression Regulation, Developmental, Growth Cones ultrastructure, Membrane Proteins metabolism, Motor Neurons ultrastructure, Muscles ultrastructure, Nerve Tissue Proteins metabolism, Nervous System growth & development, Nervous System ultrastructure, Phycoerythrin chemistry, Phylogeny, Protein Interaction Mapping methods, Protein Isoforms genetics, Protein Isoforms metabolism, Signal Transduction, Drosophila Proteins genetics, Drosophila melanogaster metabolism, Growth Cones metabolism, Membrane Proteins genetics, Motor Neurons metabolism, Muscles metabolism, Nerve Tissue Proteins genetics, Nervous System metabolism
- Abstract
An 'interactome' screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interactome screening, the Bio-Plex Interactome Assay (BPIA), which allows identification of many interactions in a single sample. Using the BPIA, we 'deorphanized' four more members of the Beat-Side network. We confirmed interactions using surface plasmon resonance. The expression patterns of beat and side genes suggest that Beats are neuronal receptors for Sides expressed on peripheral tissues. side-VI is expressed in muscle fibers targeted by the ISNb nerve, as well as at growth cone choice points and synaptic targets for the ISN and TN nerves. beat-V genes, encoding Side-VI receptors, are expressed in ISNb and ISN motor neurons. more...
- Published
- 2017
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