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The production of barley cultivars with malting and brewing quality characteristics is subject to strict grading and technical standards for the end-use market. Environmental and management factors can significantly alter grain quality, and the qualities required for malting. Crop and kernel uniformity are critical factors where variability can exceed the tolerance for meeting malt quality. A practice to address variations in crop maturity is the application of pre-harvest glyphosate. Pre-harvest glyphosate applications can, however, alter malting characteristics in barley, and, if mis-timed, can also reduce yield. A 4-year study at five locations in Alberta and Saskatchewan from 2013 to 2017 was conducted to determine the effects of pre-harvest glyphosate applications on malting barley characteristics. Glyphosate was applied at 900 and 1125 g ae ha−1 on malting barley cultivars 'CDC Meredith' and 'AC Metcalfe' malting barley at soft dough, hard dough, and physiological maturity growth stages. Yield reductions of up to 12% were observed from glyphosate applications at soft dough, and yield was maximized with applications at physiological maturity. Glyphosate application, at two rates, reduced percentage plump kernels, but did not affect kernel weight or protein concentration. The growth stage of barley plants did not provide an accurate indicator of seed moisture levels at the time of application, which motivated our conclusion that glyphosate applications can be mistimed by inaccurate indicators. The results motivate our questioning of the utility of pre-harvest glyphosate applications, given the adverse effects to barley yield and quality observed, even when applied according to the label instructions. [ABSTRACT FROM AUTHOR]

Herbicide resistance in western Canada has increased interest in alternative weed management strategies. Physical impact mills, a form of harvest weed seed control, have been identified as a strategy that may be well suited for Canadian use. The efficacy of the Harrington Seed Destructor, a physical impact mill, was evaluated in 20 producer fields in Alberta on a wide spectrum of weed species over 3 years. Significant differences in weed densities between the physical impact mill treatment and the regular harvest densities were few; however, some population density reductions were observed. Measurable reductions in weed densities may have been limited by the short timeframe of the experiment, the high initial densities of the weeds, or the targeted weed species having dormancy or longer term seedbanks. Additionally, identified knowledge gaps on how best to optimize physical impact mill efficacy may have reduced the efficacy of the physical impact mill in this study. This study showed no conclusive evidence for the efficacy of a physical impact mill on tested weed species under field conditions. However, it did provide a number of important considerations for future studies. [ABSTRACT FROM AUTHOR]

Although herbicides have been a dominant and effective weed control strategy for decades in Western Canada, herbicide resistance and the lack of new modes of action have resulted in weed management challenges. Integrated weed management strategies have been shown to be successful in controlling certain weed species that are problematic in cropping systems. The objective of this study was to investigate integrated weed management strategies that have been successful on individual species to determine their applicability to a multiple weed species that may coexist in a field. In addition, harvest weed seed control was incorporated into these integrated weed management strategies to determine its impact in western Canadian cropping systems. A 5-year rotational study was conducted from 2016 to 2020 at Beaverlodge, Lacombe, and Lethbridge, AB; Scott and Saskatoon, SK; and Carman, MB, that incorporated integrated weed management strategies such as rotational crop diversity (including winter annuals and perennials), increased seeding rates, crop silaging, chaff collection, and with or without in-crop herbicides. This research confirmed success in managing some species of weeds such as wild oat when increased seeding rates, 2 years of early cut silage barley, and competitive winter cereals were incorporated into a cropping system, even when no in-crop herbicides were applied. However, some weed growth morphologies (e.g., twining weeds) or life cycles (e.g., facultative winter annuals) were not managed successfully with this combination of strategies. Chaff collection provided incremental weed control benefits but did not serve as a replacement for herbicidal weed control. Weed densities had an apparent impact on the success of these integrated weed management strategies, suggesting that the sooner they are adopted, the more likely they are to be successful at maintaining or reducing weed densities. This study not only showed the ability to reduce reliance on herbicides with strategies that can be effective in Western Canada but also highlighted the need for further understanding of different weed species and their responses to integrated weed management strategies, as well as the complexity of managing a weed community with integrated weed management. [ABSTRACT FROM AUTHOR]

Placing N in the form of urea with the seed allows seeding and fertilizer application to be accomplished simultaneously with minimal soil disturbance. However, seedling damage can occur from excess seed-placed urea. The objective of this study was to compare the effects of seedplaced and side-banded N (urea) applied at different rates on barley (Hordeum vulgare L.) density, maturity, and yield and wild oat (Avena fatua L.) fecundity, and to investigate if increasing the barley seeding rate would improve the ability of barley to overcome urea-induced injury and compete better with wild oat. A field experiment was conducted at three locations in western Canada over 3 yr. Nitrogen was applied as urea at five rates (0, 30, 60, 90, and 120 kg [ha.sup.-1] actual N), either directly with the seed or as a side-band, at three barley seeding rates (200, 300, and 400 seeds [m.sup.-2]). When N was placed with the seed, barley plant density decreased, while time to maturity and wild oat fecundity increased as N rate increased. Barley yield also decreased but only at N rates above 60 kg [ha.sup.-1]. Placing Nas a side-band did not reduce barley density, resulting in shortened time to maturity, increased barley yield, and Iower wild oat fecundity as compared to seed-placed N. Increasing the seeding rate increased barley density and reduced time to maturity and wild oat fecundity but did not affect barley yield.

Rhizosphere microbes influence one another, forming extremely complex webs of interactions that may determine plant success. Identifying the key factors that structure the fungal microbiome of the plant rhizosphere is a necessary step in optimizing plant production. In a long-term field experiment conducted at three locations in the Canadian prairies, we tested the following hypotheses: (1) diversification of cropping systems influences the fungal microbiome of the canola (Brassica napus) rhizosphere; (2) the canola rhizosphere has a core fungal microbiome, i.e., a set of fungi always associated with canola; and (3) some taxa within the rhizosphere microbiome of canola are highly interrelated and fit the description of hub taxa. Our results show that crop diversification has a significant effect on the structure of the rhizosphere fungal community but not on fungal diversity. We also discovered and described a canola core microbiome made up of one zero-radius operational taxonomic unit (ZOTU), cf. Olpidium brassicae, and an eco-microbiome found only in 2013 consisting of 47 ZOTUs. Using network analysis, we identified four hub taxa in 2013: ZOTU14 (Acremonium sp.), ZOTU28 (Sordariomycetes sp.), ZOTU45 (Mortierella sp.) and ZOTU179 (cf. Ganoderma applanatum), and one hub taxon, ZOTU17 (cf. Mortierella gamsii) in 2016. None of these most interacting taxa belonged to the core microbiome or eco-microbiome for each year of sampling. This temporal variability puts into question the idea of a plant core fungal microbiome and its stability. Our results provide a basis for the development of ecological engineering strategies for the improvement of canola production systems in Canada. [ABSTRACT FROM AUTHOR]

The rhizosphere hosts a complex web of prokaryotes interacting with one another that may modulate crucial functions related to plant growth and health. Identifying the key factors structuring the prokaryotic community of the plant rhizosphere is a necessary step toward the enhancement of plant production and crop yield with beneficial associative microorganisms. We used a long-term field experiment conducted at three locations in the Canadian prairies to verify that: (1) the level of cropping system diversity influences the α- and β-diversity of the prokaryotic community of canola (Brassica napus) rhizosphere; (2) the canola rhizosphere community has a stable prokaryotic core; and (3) some highly connected taxa of this community fit the description of hub-taxa. We sampled the rhizosphere of canola grown in monoculture, in a 2-phase rotation (canola-wheat), in a 3-phase rotation (pea-barley-canola), and in a highly diversified 6-phase rotation, five and eight years after cropping system establishment. We detected only one core bacterial Amplicon Sequence Variant (ASV) in the prokaryotic component of the microbiota of canola rhizosphere, a hub taxon identified as cf. Pseudarthrobacter sp. This ASV was also the only hub taxon found in the networks of interactions present in both years and at all three sites. We highlight a cohort of bacteria and archaea that were always connected with the core taxon in the network analyses. [ABSTRACT FROM AUTHOR]