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Huseyin Demirbilek,1 Dogus Vuralli,1 Basma Haris,2 Khalid Hussain2 1Department of Pediatric Endocrinology, Hacettepe University Faculty of Medicine, Ankara, Turkey; 2Department of Pediatric Endocrinology, Sidra Medicine, Doha, QatarCorrespondence: Khalid Hussain, Sidra Medicine, OPC, C6-340, PO Box 26999, Al Luqta Street, Education City North Campus, Doha, Qatar, Tel +974-4003-7608, Email khussain@sidra.orgAbstract: Hypoglycaemia is common in patients with diabetes mellitus and is a limiting factor for achieving adequate glycaemic control. In the vast majority of cases, hypoglycaemia develops due to the imbalance between food intake and insulin injections. As recurrent hypoglycaemia leads to significant morbidity and mortality, the recognition and immediate treatment of hypoglycaemia in diabetic patients is thus important. In the last 20 years, the introduction of improved insulin analogues, insulin pump therapy, continuous glucose monitoring (CGM), and sensor-augmented pump therapy have all made significant improvements in helping to reduce and prevent hypoglycaemia. In terms of treatment, the American Diabetes Association recommends oral glucose as the first-line treatment option for all conscious patients with hypoglycaemia. The second line of treatment (or first line in unconscious patients) is the use of glucagon. Novel formulations of glucagon include the nasal form, the Gvoke HypoPen which is a ready-to-deliver auto-injector packaged formulation and finally a glucagon analogue, Dasiglucagon. The Dasiglucagon formulation has recently been approved for the treatment of severe hypoglycaemia. It is a ready-to-use, similar to endogenous glucagon and its potency is also the same as native glucagon. It does not require reconstitution before injection and therefore ensures better compliance. Thus, significant improvements including development of newer insulin analogues, insulin pump therapy, continuous glucose monitoring (CGM), sensor-augmented pump therapy and novel formulations of glucagon have all contributed to reducing and preventing hypoglycaemia in diabetic individuals. However, considerable challenges remain as not all patients have access to diabetes technologies and to the newer glucagon formulations to help reduce and prevent hypoglycaemia.Keywords: hypoglycaemia, type 1 diabetes, type 2 diabetes, glucagon, counterregulatory hormone

Basma Haris,1 Samuel Stafrace,2 Khalid Hussain1 1Department of Pediatric Endocrinology, Sidra Medicine, Doha, Qatar; 2Department of Diagnostic Imaging, Sidra Medicine, Doha, QatarCorrespondence: Khalid Hussain, Department of Pediatric Medicine, Sidra Medicine, OPC, C6-340|PO Box 26999, Al Luqta Street, Doha, Qatar, Tel +974-4003-7608 ; +974-30322007, Email khussain@sidra.orgAbstract: Type 2 diabetes is a chronic disease due to insulin resistance resulting in hyperglycemia. The prevalence of type 2 diabetes is increasing worldwide in the pediatric population. In the pediatric population, type 2 diabetes typically develops around adolescence; however, patients with a younger age of onset are now being reported. Earlier onset of type 2 diabetes is associated with a more aggressive course of disease and earlier comorbidities, although data on this is limited. We report a child from Qatar with type 2 diabetes that was diagnosed at 7 years of age, along obesity with a BMI of 26.8 kg/m2. Elevated liver enzymes, c-peptide, and insulin levels were observed along with fatty liver on an ultrasound. The child had severe acanthosis nigricans with increased appetite. There was a positive family history for type 2 diabetes. Testing for type 1 diabetes autoantibodies, monogenic obesity, and monogenic diabetes screening was negative. This is the second youngest child reported to have type 2 diabetes. Accurate diagnosis, early reporting, and long-term follow-up of such cases is necessary to bring more attention to the subgroup of type 2 diabetes in very young patients.Keywords: pediatric diabetes, type 2 diabetes, insulin resistance, early-onset diabetes mellitus

Basma Haris,1 Idris Mohammed,1 Sara Al-Khawaga,2 Khalid Hussain1 1Department of Pediatric Endocrinology, Sidra Medicine, Education City, Doha, Qatar; 2Department of Dermatology, Hamad General Hospital, Doha, QatarCorrespondence: Khalid HussainDepartment of Pediatric Medicine, Sidra Medicine, Education City, OPC, C6-340 |PO Box 26999, Al Luqta Street, North Campus, Doha, Qatar, Tel +974-4003-7608, Email khussain@sidra.orgPurpose: To report a family with a homozygous INS promotor gene mutation causing permanent neonatal diabetes mellitus (PNDM) in one sibling and autoantibody negative childhood onset diabetes in another sibling.Case Presentation: Patient 1 is a 12-year-old girl born at term with low birth weight to a consanguineous family, diagnosed with PNDM at 26 days of life. She presented with ketoacidosis and has a severe course of disease with high insulin requirement. Patient 2 is a 9-year-old girl born at term with normal weight, who presented with ketoacidosis at 2 years of age. Both subjects have negative type 1 autoantibodies. On genetic testing, a mutation in the promoter region of INS gene c.-331 C>G was found in homozygous state in both subjects and in a heterozygous state in parents.Conclusion: Homozygous INS gene promotor mutations may present with either PNDM or later onset autoantibody negative diabetes in childhood. This suggests that homozygous INS gene promotor mutations show marked heterogeneity in clinical presentation within individuals in the same family. The pathophysiology of this is not well known but could be related to a number of factors, including the position of the variant, penetrance, other associated genetic defects, HLA etc. Premarital screening and genetic counselling is recommended for highly consanguineous families to reduce occurrence of such conditions.Keywords: pediatric diabetes, neonatal diabetes mellitus, type 1b diabetes, INS mutation

Introduction: Oral cancer is often associated with various factors, such as betel nut consumption, which usually causes specific premalignant lesions. The most common oral cancer is oral squamous cell carcinoma (OSCC) which has a low 5-year survivor rate because early detection of the malignancies is not widely used and not routinely carried out in dental practice. Early detection of malignancy can be done by measuring the salivary Ki-67 level and micronucleus assay from the buccal smear. Aims and Objectives: The study aimed to examine the potency of the salivary Ki-67 level and micronucleus assay for early detection of OSCC in betel nut chewers. Materials and Methods: This study was conducted in 17 betel nut chewers and 17 healthy people as a control group. Saliva was collected with the passive drooling technique and then analyzed using Enzyme-linked immunosorbent assay (ELISA). Buccal smears were taken, then a cytological slide was made and stained using Papanicolaou. Settings and Design: This study was a cross-sectional analytic survey that was conducted in the Banyumas District of Indonesia with a post-test- only control group design. Statistical Analysis: The statistical analysis used is a non-parametric test using Mann–Whitney and Kruskal–Wallis tests. Results: There was a significant difference between the Ki-67 level and micronucleus in the betel nut chewers group and the control group. There was a significant difference between Ki-67 and micronucleus levels in the various types of oral lesions that were found in the betel nut chewer's group. Conclusion: Examination of Ki-67 and micronucleus assay is effective as an alternative early biomarker for OSCC.

Background: Periodontitis is a chronic multifactorial disease caused by microorganisms such as G-anaerobes in the periodontal tissues. It activates host defense cells and releases inflammatory mediators such as tumor necrosis factor-alpha (TNF-α) and prostaglandin E2 (PGE2). Ganoderma lucidum is a traditional medicinal mushroom with anti-inflammatory effects against various diseases. Biologically, different levels of its active constituents, such as triterpenoids and phenolic compounds, reduce inflammation with various pathways. Furthermore, the constituents inhibit toll-like receptor 4, MyD88 receptors, and the activities of nuclear factor-kappa B, which synthesize TNF-α and PGE2. Purpose: This study aimed to analyze the extent to which G. lucidum extract can reduce TNF-α and PGE2 levels in periodontitis model Sprague Dawley rats. Methods: Thirty Sprague Dawley rats were randomly divided into six groups of five rats. Periodontitis inflammation was induced by the injection of Porphyromonas gingivalis bacteria into intrasulcular gingival incisors in the lower jaw labial section. Grouping was as follows: Group K1 (healthy control); Group K2 (negative control); Group K3 (positive control with doxycycline dose 0.27 mg/kg BW); Group P1 (G. lucidum extract dose 5 mg/kg BW); Group P2 (G. lucidum extract dose 10 mg/kg BW); and Group P3 (G. lucidum extract dose 20 mg/kg BW). Samples were taken from rat gingival tissue and the levels of TNF-α and PGE2 were examined using the enzyme-linked immunosorbent assay method. Data analysis was performed using one-way analysis of variance (ANOVA) with a confidence level of 95% (p < 0.05). Results: The levels of TNF-α and PGE2 were the highest in the K2 group and the lowest in the K1 group. One-way ANOVA showed no significant difference in TNF-α and PGE2 levels between P3 and K1 group. Conclusion: G. lucidum extract can reduce TNF-α and PGE2 levels in Sprague Dawley rats with periodontitis.

Overlapping or multiple certificates occur because there are two certificates on the same land and are officially issued by the National Land Office. As a result this overlap raises a dispute between the parties, and then the certificate is no longer valid evidence. The formulation of the problem is the regulation of overlapping dispute resolution according to legislation in Indonesia and how the authority of the Land Office in resolving disputes overlapping land parcels. The research method used is empirical juridical using primary and secondary data. The results showed that there is no specific regulation regarding disputes over double or overlapping certificates, currently the regulations used by the District Land Office still refer to the Regulation of the Minister of Agrarian Affairs and Spatial Planning number 11 of 2016 concerning Settlement of Land Cases. To reinforce the authority of the Land Office in resolving cases of multiple certificates, a special regulation on the settlement of overlapping land cases is needed. The conclusion shows that the Land Office has carried out its role by its duties, principal, and functions, but because the regulations used are general, many obstacles are found in solving the problem of multiple certificates, therefore there is a need for legal reform and education from the government to the public about the importance of officially registering land.

IntroductionExcessive alcohol consumption is a leading global risk factor for ill-health and premature death. Digital alcohol interventions can be effective at reducing alcohol consumption, but their widespread adoption is lagging behind. This study aimed to identify factors promoting or inhibiting the implementation of a digital alcohol intervention in Norwegian primary care, by using Normalization Process Theory (NPT).MethodsA mixed methods feasibility study combining quantitative and qualitative methods. A digital alcohol intervention called “Endre” was implemented across four GP practices in Stavanger and Oslo. Usage of the intervention was logged on the digital platform. General practitioners (GPs) reported their perceived uptake of the intervention via a web-based survey. The Normalization MeAsure Development (NoMAD) survey was used to measure support staff's perceived normalization of the intervention. Qualitative data were analyzed using the NPT framework, with quantitative data analyzed descriptively and using χ2 and Wilcoxon signed-rank test for differences in current and future normalization.ResultsThirty-seven GPs worked in the clinics and could recruit patients for the digital intervention. Thirty-six patients registered for the intervention. Nine patients dropped out early and 25 completed the intervention as intended. Low normalization scores at follow-up (n = 27) indicated that Endre did not become fully embedded in and across practices. Nonetheless, staff felt somewhat confident about their use of Endre and thought it may become a more integral part of their work in the future. Findings from six semi-structured group interviews suggested that limited implementation success may have been due to a lack of tailored implementation support, staff's lack of involvement, their diminished trust in Endre, and a lack of feedback on intervention usage. The outbreak of the Covid-19 pandemic further limited opportunities for GPs to use Endre.ConclusionThis study investigated the real-world challenges of implementing a digital alcohol intervention in routine clinical practice. Future research should involve support staff in both the development and implementation of digital solutions to maximize compatibility with professional workflows and needs. Integration of digital solutions may further be improved by including features such as dashboards that enable clinicians to access and monitor patient progress and self-reported outcomes.

The pragmatic analysis has become progressively noteworthy in understanding impoliteness strategy in recent years, especially in the movie pragmatics analysis. This study examines the types of impoliteness strategies used in the movie Turning Red. The method of qualitative design is qualified to deal with it. The data was collected by watching the movie repeatedly and taking notes during the conversation. The data were analyzed using impoliteness strategies in the Culpeper framework. The result showed that thirteen utterances consisted of three utterances of baldness on record, four utterances of positive impoliteness, five utterances of negative impoliteness, and one utterance identified as sarcasm or mock politeness. However, there is one strategy that is not found in the movie: withholding politeness. It means that this movie is intended to show the negative facial desires and put the environment in negative situations, interfering with their freedom of action and humiliation.

Urban Farming is one of the important Bekasi City Government’s programs to strengthen the food security, considering the increase in agricultural land conversion. This research aims to determine the internal and external factors influencing the development of community’s hydroponic vegetable business (HVB), alternative strategies for developing the community’s HVB, and priority order of competitive strategies to be implemented in the development of the community’s HVB in Bekasi City. Descriptive method was used in this research, supported by data analysis using IFE, EFE, SWOT, IE, and QSPM matrices. The total IFE matrix score was 2.4, indicated the hydroponic community's average ability in responding to the internal environment. The total EFE matrix score was 1.98, showed its low ability to respond to the external. The IE matrix implied the hydroponic community’s position in cell VIII or in the harvest and divest position. Based on the SWOT matrix, four alternative strategies have been obtained in priority order, which also have been analyzed by using the QSPM matrix; (1) revitalization of the urban farming-development program, (2) market facilitation of hydroponic community production, (3) promotion of hydroponic vegetables as fresh vegetables, and (4) education for hydroponic community.

This paper presents the results of a two-year trial on the yield, total phenolic and flavonoid contents and total antioxidant capacity of the 'Willamette' red raspberry under organic and conventional farming. A trial was conducted in a commercial plantation of 'Willamette' raspberries located in the southwestern part of Serbia. The total phenolics and flavonoids in the raspberry fruits were determined by the Folin-Ciocalteu reagent and aluminium chloride assay, respectively. The ferric reducing antioxidant power assay was used to evaluate the total antioxidant capacity of the raspberry fruits. The results of this study showed that the raspberry yields were significantly affected by the different farming systems. The raspberry yield in conventional farming was higher than that of organic farming. Contrastingly, the raspberries from the organic farming contained higher levels of the total phenolics and flavonoids and also had a higher total antioxidant activity compared to those from the conventional farming in both 2021 and 2022. This study also showed that the total phenolic and flavonoid contents of the 'Willamette' red raspberry have a significant and positive correlation with their total antioxidant capacity, indicating that the phenolic compounds play an important role in the antioxidant activity of the raspberry.

The effective management of natural resources is critical in achieving environmental sustainability, and it requires a careful balance of ecological and economic interests. Therefore, this study analyzed the effectiveness of village government policy in managing post-mining land for environmental sustainability using a non-doctrinal legal approach. Primary data were collected through interviews and observations, while secondary data came from regulations, scientific journals, and papers related to post-mining land rehabilitation governance. The study was conducted in Sukageuri View, Kuningan Regency. The study found that the policy implemented by the Cisantana Village Government in managing post-mining land through the establishment of the Village-Owned Enterprise (VOE) in 2016 has been effective. The area, formerly known as Sukageuri View, is home to various flora and fauna species, with several businesses operating and over 114,000 visitors engaging in social activities in 2022. Additionally, the site has contributed to the village's original income and supported the work program of the Cisantana Village Government. In conclusion, the policy implemented by the Cisantana Village Government effectively balances ecological, social, and economic factors, meeting the criteria for environmental sustainability.

Abstract COVID-19 is characterised by systemic immunological perturbations in the human body, which can lead to multi-organ damage. Many of these processes are considered to be mediated by the blood. Therefore, to better understand the systemic host response to SARS-CoV-2 infection, we performed systematic analyses of the circulating, soluble proteins in the blood through global proteomics by mass-spectrometry (MS) proteomics. Here, we show that a large part of the soluble blood proteome is altered in COVID-19, among them elevated levels of interferon-induced and proteasomal proteins. Some proteins that have alternating levels in human cells after a SARS-CoV-2 infection in vitro and in different organs of COVID-19 patients are deregulated in the blood, suggesting shared infection-related changes.The availability of different public proteomic resources on soluble blood proteome alterations leaves uncertainty about the change of a given protein during COVID-19. Hence, we performed a systematic review and meta-analysis of MS global proteomics studies of soluble blood proteomes, including up to 1706 individuals (1039 COVID-19 patients), to provide concluding estimates for the alteration of 1517 soluble blood proteins in COVID-19. Finally, based on the meta-analysis we developed CoViMAPP, an open-access resource for effect sizes of alterations and diagnostic potential of soluble blood proteins in COVID-19, which is publicly available for the research, clinical, and academic community.

The purpose of this study is to examine the possibility of GO to be used as an adsorbent for five novel potentially hazardous azo-dyes for their removal from aqueous solution. Adsorption characteristics of GO for azo-dyes removal were investigated by means of experimental and computational DFT as well as Monte Carlo approaches. Experimental studies include the effect of adsorbent dose, contact time, and initial concentration, while computational investigation involves DFT and Monte Carlo (MC) simulations. Through DFT studies geometric, electronic, and thermodynamic parameters were explored and possible mechanism of interactions and adsorption energies by predicted through MC by searching lowest possible adsorption complexes. Experimental data were evaluated by Langmuir models in order to describe the equilibrium isotherms. Equilibrium data fitted well to the Langmuir model. Thermodynamic parameters i.e., free energy change, enthalpy change, and entropy change revealed that the removal of azo-dyes by adsorption on the surface of GO molecular sieves was spontaneous. Nature of the process was found to be physiosorption involving non-covalent interaction. The study unveiled that GO can be used as an efficient adsorbent material for the adsorption of azo-dyes from aqueous solution.

Indonesia has enacted a number of regulations dealing with issues of mining governance, and governance. However, the issuance of these various laws and regulations has resulted in illegal mining crimes resulting in environmental damage. The purpose of this study is to analyze sustainable mining governance policies in Indonesia, Africa and Germany. This research method uses a doctrinal legal approach. Resources have been compiled through an examination of mineral and coal mining laws and regulations, governance and environmental governance regulations, as well as reports from various authorities on the same subject. The results of this study show how Indonesia's previous mining law policies recognized local governments as the authority for licensing and monitoring mining activities. This policy was revoked based on the latest governance regulations and had an impact on the vacancy in monitoring mining activities at the district/city level, as a result of this vacancy there was environmental damage due to illegal mining and bad mining activities so that it was necessary to strengthen government policies in monitoring mineral and coal mining. Mining governance policies in Indonesia, Africa and Germany are currently starting to lead to environmental sustainability.

France is Europe’s leading producer of flaxseed. This seed is rich in omega-3, energy, and protein for animals, but it also contains anti-nutritional factors such as mucilage. Thus, mucilage must be removed and could be used as a bio-admixture in cementitious materials development, reducing the environmental impact of cementitious materials. This study aims to valorize the usage of flaxseed mucilage (FM) in ordinary Portland cement. FM caused macroscopic and microscopic changes in the materials studied. The higher the concentration, the greater the changes were. The admixed samples showed an exponentially concentration-dependent delay in setting. FM degradation products induced by the cementitious conditions accentuated the delay. However, this delay in setting did not affect the hydrates’ growth in the material. In fact, FM showed a “delay accelerator” behavior, meaning that once hydration began, it was accelerated as compared to a reference. Macroscopically, FM induced significant flocculation, increasing material porosity and carbonation. Consequently, bulk density and thermal conductivity were reduced. At the highest amount of FM admixture (0.75% w/w), FM allowed bridge formation between Ca(OH)2 crystals, which can improve the mechanical properties of mortars. Because FM is highly hygroscopic, it has the capability to absorb water and subsequently release it gradually and under controlled conditions into the cement matrix. Therefore, regulation of water diffusion from the mucilage may induce the self-healing properties responsible for mechanical properties similar to that of the reference in the medium to long term.

One physical sign of a person who has Alzheimer's is the diminution of the area of the hippocampus and ventricles. A good quality magnetic resonance imaging (MRI) will provide a high-quality image so that the doctor will quickly analyze the abnormalities of the hippocampus and ventricle area. However, for low-quality MRI, this is difficult to do. This condition will be a significant problem for some regions in developing countries including Indonesia, where many hospitals have only low-quality MRI, and many hospitals do not have them at all. The primary purpose of this research is to develop simple tools to analyze morphological characteristics in Alzheimer's patients. In this paper, we focus only on coronal slice analysis. We will use watershed method segmentation, because of this method able to segment the boundaries automatically, so that parts of the hippocampus and ventricles can be identified in an MRI image. Analysis of morphological characteristics is also classified by age and gender. Then by referring to the value of the clinical dementia rating (CDR), the process of identifying between images with Alzheimer's disease (AD) and healthy models is done based on the morphological analysis that has been done. The results show this method has a better performance compared to the previously work. [ABSTRACT FROM AUTHOR]

Abstract Background COVID-19 remains a major public health challenge, requiring the development of tools to improve diagnosis and inform therapeutic decisions. As dysregulated inflammation and coagulation responses have been implicated in the pathophysiology of COVID-19 and sepsis, we studied their plasma proteome profiles to delineate similarities from specific features. Methods We measured 276 plasma proteins involved in Inflammation, organ damage, immune response and coagulation in healthy controls, COVID-19 patients during acute and convalescence phase, and sepsis patients; the latter included (i) community-acquired pneumonia (CAP) caused by Influenza, (ii) bacterial CAP, (iii) non-pneumonia sepsis, and (iv) septic shock patients. Results We identified a core response to infection consisting of 42 proteins altered in both COVID-19 and sepsis, although higher levels of cytokine storm-associated proteins were evident in sepsis. Furthermore, microbiologic etiology and clinical endotypes were linked to unique signatures. Finally, through machine learning, we identified biomarkers, such as TRIM21, PTN and CASP8, that accurately differentiated COVID-19 from CAP-sepsis with higher accuracy than standard clinical markers. Conclusions This study extends the understanding of host responses underlying sepsis and COVID-19, indicating varying disease mechanisms with unique signatures. These diagnostic and severity signatures are candidates for the development of personalized management of COVID-19 and sepsis.

Glioblastoma (GBM) cancer stem cells (GSCs) contribute to GBM's origin, recurrence, and resistance to treatment. However, the understanding of how mRNA expression patterns of GBM subtypes are reflected at global proteome level in GSCs is limited. To characterize protein expression in GSCs, we performed in‐depth proteogenomic analysis of patient‐derived GSCs by RNA‐sequencing and mass‐spectrometry. We quantified > 10 000 proteins in two independent GSC panels and propose a GSC‐associated proteomic signature characterizing two distinct phenotypic conditions; one defined by proteins upregulated in proneural and classical GSCs (GPC‐like), and another by proteins upregulated in mesenchymal GSCs (GM‐like). The GM‐like protein set in GBM tissue was associated with necrosis, recurrence, and worse overall survival. Through proteogenomics, we discovered 252 non‐canonical peptides in the GSCs, i.e., protein sequences that are variant or derive from genome regions previously considered non‐protein‐coding, including variants of the heterogeneous ribonucleoproteins implicated in RNA splicing. In summary, GSCs express two protein sets that have an inverse association with clinical outcomes in GBM. The discovery of non‐canonical protein sequences questions existing gene models and pinpoints new protein targets for research in GBM.

Background: Differences in histopathological features that describe the growth mechanism and biological behaviour of follicular and plexiform ameloblastomas are associated with benign, aggressive and destructive tumour markers. p16 has inhibitory interactions between cyclin D and CDK 4/6 to block the cell cycle and alterations related to severity. Purpose: This study intends to evaluate and determine differential expressions of p16 protein in follicular and plexiform ameloblastomas. Methods: This is a descriptive analytics study. A total of 21 specimens consisting of follicular and plexiform ameloblastomas and healthy gingiva tissues as the negative control were examined using the immunohistochemistry assay. The analysis of p16 protein expression was interpreted by immunoreactive scoring. Statistical analysis was conducted using SPSS software with the Mann–Whitney test. A p-value 0.05). Conclusion: The highest intensity of p16 protein expression was found in the plexiform type, even though it was not significantly different from the follicular type ameloblastoma.

Tujuan penelitian ini adalah untuk mendesain dan mengimplementasikan media pembelajaran interaktif berbantuan Swishmax-4 materi garis lurus. Jenis penelitian yang digunakan dalam penelitian ini adalah Research and Development, dengan menggunakan model penelitian ADDIE. Teknik pengumpulan adalah Wawancara, Angket dan Tes. Data penelitian berupa hasil pretest dan posttest dianalisis menggunakan rumus effect size. Hasil penelitian menunjukkan bahwa media pembelajaran yang dikembangkan sangat valid, ahli materi memperoleh skor rata-rata 3,73 dan ahli media skor rata-rata 3,27. Respon mahasiswa memperoleh hasil skor rata-rata 3,49 pada uji skala kecil dan 3,42 pada uji skala besar dengan criteria keduanya sangat menarik. Keefektifan media pembelajaran diuji mengunakan effect size yaitu dengan perolehan skor efektifitas 0,78 dengan kategori efektivitas sedang.

This paper examines the questions of time (transience) and space (persistence) in architecture and culture. The endurance and relevance of these topics are best seen in the integration of architectural heritage into contemporary life. The paper elaborates this by applying a methodological-applicative model to the renovation of the modernist Corn-Markesic villa in Sarajevo’s Crni Vrh housing estate, which has been designated a national monument of Bosnia and Herzegovina. The model seeks to achieve a practical and architecturally creative solution for the villa’s adaptations. Although changing the Crni Vrh urban context might change the appearance and values of the villa, the opposite is also true: the adaptation of the villa to meet contemporary requirements may change its current context. This paper examines the character of the villa’s renovation though field research and the development of an architectural design, to reflect present and future needs, but still faithfully represent the past. The suggested adaptation model follows the lifecycle of a family, and questions the possibilities of transformation at the point at which certain spatial structures and content are no longer needed. The design and research process adheres to guidelines in the European Cultural Heritage Green Paper.