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19. Two Novel Betarhabdovirids Infecting Ornamental Plants and the Peculiar Intracellular Behavior of the Cytorhabdovirus in the Liana Aristolochia gibertii

Author

Ramos-González, Pedro Luis, primary, Vaz Alexandre, Maria Amelia, additional, Potsclam-Barro, Matheus, additional, Lembo Duarte, Lígia Maria, additional, Michea Gonzalez, Gianluca L., additional, Chabi-Jesus, Camila, additional, Ramos, Alyne F., additional, Harakava, Ricardo, additional, Lorenzi, Harri, additional, Freitas-Astúa, Juliana, additional, and Kitajima, Elliot Watanabe, additional

Published
2024
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24. The complete genome sequence of Candidatus Liberibacter americanus, a bacterium associated with Citrus Huanglongbing in Brazil

Author

Wulff, Nelson A., Zhang, Shujian, Martins, Elaine, Setubal, João, Kumar, Dibyendu, Foissac, Xavier, Almeida, Nalvo F., Harakava, Ricardo, Bové, Joseph M., and Gabriel, Dean

Abstract

We used PFGE followed by CsCl bisbenzymide centrifugation to obtain sufficient DNA for pyrosequencing of the Ca. Liberibacter americanus (Lam) strain “São Paulo” genome. The complete circular genomic DNA sequence of Lam is 1,195,201 bp, with an average GC content of 31.12%, somewhat lower than other Liberibacters. There are 1,056 predicted Lam genes, with 1,002 encoding proteins, 9 encoding rRNA genes and 45 encoding tRNAs. The overall gene organization and structure of the Lam genome is more similar to Lso than to Las. There are 951 genes common to Lam, Lso and Las, 27 genes found in Lam and Lso but not Las, and only 8 genes common to Lam and Las but not found in Lso. Many pseudogenes or truncated genes were found among the unique genes of all 3 species. As with Las, two prophage were confirmed in Lam, with SP2 being 39,941 bp and SP1 being 16,398 bp in size; as in Las, the one that appears to replicate as an excision plasmid prophage carries putative lysogenic conversion genes, specifically peroxidases and a Type Vc secreted adhesin. These predicted peroxidases and adhesin were found in both Las and Lam, but appeared fragmentary or degenerated in Lso, indicating their potential for citrus host range determination. Liberibacter genomes seem to be under selective pressure to reduce GC% content and to lose unimportant genes. Although Lam have an outer membrane, most of the genes required for biosynthesis of lipopolysaccharide, which can trigger ROS production, are missing from the Lam genome.

Published
2014

25. Two Novel Betarhabdovirins Infecting Ornamental Plants and the Peculiar Intracellular Behavior of the Cytorhabdovirus in the Liana Aristolochia gibertii.

Author

Ramos-González, Pedro Luis, Alexandre, Maria Amelia Vaz, Potsclam-Barro, Matheus, Duarte, Lígia Maria Lembo, Michea Gonzalez, Gianluca L., Chabi-Jesus, Camila, Ramos, Alyne F., Harakava, Ricardo, Lorenzi, Harri, Freitas-Astúa, Juliana, and Kitajima, Elliot Watanabe

Subjects
ORNAMENTAL plants, ARISTOLOCHIA, LIANAS, HERBACEOUS plants, CELL nuclei, ROOTSTOCKS
Abstract

Two novel members of the subfamily Betarhabdovirinae, family Rhabdoviridae, were identified in Brazil. Overall, their genomes have the typical organization 3′-N-P-P3-M-G-L-5′ observed in mono-segmented plant-infecting rhabdoviruses. In aristolochia-associated cytorhabdovirus (AaCV), found in the liana aristolochia (Aristolochia gibertii Hook), an additional short orphan ORF encoding a transmembrane helix was detected between P3 and M. The AaCV genome and inferred encoded proteins share the highest identity values, consistently < 60%, with their counterparts of the yerba mate chlorosis-associated virus (Cytorhabdovirus flaviyerbamate). The second virus, false jalap virus (FaJV), was detected in the herbaceous plant false jalap (Mirabilis jalapa L.) and represents together with tomato betanucleorhabdovirus 2, originally found in tomato plants in Slovenia, a tentative new species of the genus Betanucleorhabdovirus. FaJV particles accumulate in the perinuclear space, and electron-lucent viroplasms were observed in the nuclei of the infected cells. Notably, distinct from typical rhabdoviruses, most virions of AaCV were observed to be non-enclosed within membrane-bounded cavities. Instead, they were frequently seen in close association with surfaces of mitochondria or peroxisomes. Unlike FaJV, AaCV was successfully graft-transmitted to healthy plants of three species of the genus Aristolochia, while mechanical and seed transmission proved unsuccessful for both viruses. Data suggest that these viruses belong to two new tentative species within the subfamily Betarhabdovirinae. [ABSTRACT FROM AUTHOR]

Published
2024
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26. Bacterial Isolated from Oreochromis niloticus and Possible Use as Probiotic

Author

Sato, Luciana Yuri, primary, Dias, Danielle de Carla, additional, Natori, Mariene Miyoko, additional, Alarcon, Miguel Frederico Fernandez, additional, Ranzani-Paiva, Maria José, additional, Cassiano, Luara Lucena, additional, Ishikawa, Carlos Massatoshi, additional, Guimarães, Mateus Cardoso, additional, Moriñigo, Miguel Angel, additional, Tapia-Paniagua, Silvana, additional, Bach, Erna Elisabeth, additional, Harakava, Ricardo, additional, and Tachibana, Leonardo, additional

Published
2023
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27. First Report of Aeromonas veronii as an Emerging Bacterial Pathogen of Farmed Nile Tilapia (Oreochromis niloticus) in Brazil

Author

dos Santos, Sandie Bispo, primary, Alarcon, Miguel Fernandez, additional, Ballaben, Anelise Stella, additional, Harakava, Ricardo, additional, Galetti, Renata, additional, Guimarães, Mateus Cardoso, additional, Natori, Mariene Miyoko, additional, Takahashi, Leonardo Susumu, additional, Ildefonso, Ricardo, additional, and Rozas-Serri, Marco, additional

Published
2023
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35. Citrus Bright Spot Virus: A New Dichorhavirus, Transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil

Author

Chabi-Jesus, Camila, primary, Ramos-González, Pedro Luis, additional, Tassi, Aline Daniele, additional, Rossetto Pereira, Laura, additional, Bastianel, Marinês, additional, Lau, Douglas, additional, Canale, Maria Cristina, additional, Harakava, Ricardo, additional, Novelli, Valdenice Moreira, additional, Kitajima, Elliot Watanabe, additional, and Freitas-Astúa, Juliana, additional

Published
2023
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38. First genome sequence of an isolate of hibiscus chlorotic ringspot virus from the Western hemisphere

Author

Ramos-González, Pedro Luis, da Costa-Rodrigues, Mariane, Potsclam-Barro, Matheus, Chabi-Jesus, Camila, Banguela-Castillo, Alexander, Harakava, Ricardo, Kitajima, Elliot W., and Freitas-Astúa, Juliana

Abstract

For the first time, the near-complete genome sequence of the betacarmovirus, hibiscus chlorotic ringspot virus (HCRSV) isolated from the Americas is disclosed. High throughput sequencing of the total RNA extract from a Hibiscus-rosa sinensis(L.) plant collected in São Paulo state, Brazil, revealed the genome sequence of HCRSV isolate SBO1, which is 3945 nucleotides long and shows 93.1% nucleotide sequence identity with HCRSV_Singapore (X86448), considered the type member of the species. These two viruses display a similar genomic organization and potentially encode seven open reading frames (ORFs). In addition, a phylogenetic analysis based on a fragment with 557 nts of p38, the coat protein gene, from a cohort of 14 samples collected in Brazil revealed a no clearly defined segregation of South American isolates from those previously detected in geographical areas outside this continent. The practical consequences of the use of p38ORF-derived amplicons for detection and variability studies of HCRSV are concisely discussed.

Published
2024
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39. Assessment of the Entomopathogenic Potential of Fungal and Bacterial Isolates from Fall Armyworm Cadavers Against Spodoptera frugiperdaCaterpillars and the Adult Boll Weevil, Anthonomus grandis

Author

Dos Santos Moreira, Lidiane Maria, Marinho, Luciene Silva, Neves, Robério Carlos Santos, Harakava, Ricardo, Bessa, Layara Alexandre, and Vitorino, Luciana Cristina

Abstract

Increased attention is being focused on the biological control of agricultural pests using microorganisms, owing to their potential as a viable substitute for chemical control methods. Insect cadavers constitute a potential source of entomopathogenic microorganisms. We tested whether bacteria and fungi isolated from Spodoptera frugiperda(JE Smith) cadavers could affect its survival, development, egg-laying pattern, and hatchability, as well as induce mortality in Anthonomus grandisBoheman adults. We isolated the bacteria Enterobacter hormaecheiand Serratia marcescensand the fungi Scopulariopsissp. and Aspergillus nomiaefrom fall armyworm cadavers and the pest insects were subjected to an artificial diet enriched with bacteria cells or fungal spores to be tested, in the case of S. frugiperda, and only fungal spores in the case of A. grandis. Enterobacterhormaecheiand A. nomiaewere pathogenic to S. frugiperda, affecting the survival of adults and pupae. The fungus Scopulariopsissp. does not affect the survival of S. frugiperdacaterpillars and pupae; however, due to late action, moths and eggs may be affected. Aspergillusnomiaealso increased mortality of A. grandisadults, as well as the development of S. frugiperdain the early stages of exposure to the diet, as indicated by the vertical spore transfer to offspring and low hatchability. Enterobacterhormaecheiand A. nomiaeare potential biocontrol agents for these pests, and warrant further investigation from a toxicological point of view and subsequently in field tests involving formulations that could improve agricultural sustainability practices.

Published
2024
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41. First Report of Aeromonas veronii as an Emerging Bacterial Pathogen of Farmed Nile Tilapia (Oreochromis niloticus) in Brazil.

Author

Bispo dos Santos, Sandie, Fernandez Alarcon, Miguel, Ballaben, Anelise Stella, Harakava, Ricardo, Galetti, Renata, Guimarães, Mateus Cardoso, Natori, Mariene Miyoko, Takahashi, Leonardo Susumu, Ildefonso, Ricardo, and Rozas-Serri, Marco

Subjects
NILE tilapia, AEROMONAS, GRAM-negative anaerobic bacteria, FISH pathogens, BACTERIAL growth
Abstract

Brazil is one of the world's leading producers of Nile tilapia, Oreochromis niloticus. However, the industry faces a major challenge in terms of infectious diseases, as at least five new pathogens have been formally described in the last five years. Aeromonas species are Gram-negative anaerobic bacteria that are often described as fish pathogens causing Motile Aeromonas Septicemia (MAS). In late December 2022, an epidemic outbreak was reported in farmed Nile tilapia in the state of São Paulo, Brazil, characterized by clinical signs and gross pathology suggestive of MAS. The objective of this study was to isolate, identify, and characterize in vitro and in vivo the causative agent of this epidemic outbreak. The bacterial isolates were identified as Aeromonas veronii based on the homology of 16S rRNA (99.9%), gyrB (98.9%), and the rpoB gene (99.1%). A. veronii showed susceptibility only to florfenicol, while it was resistant to the other three antimicrobials tested, oxytetracycline, enrofloxacin, and amoxicillin. The lowest florfenicol concentration capable of inhibiting bacterial growth was ≤0.5 µg/mL. The phenotypic resistance of the A. veronii isolate observed for quinolones and tetracycline was genetically confirmed by the presence of the qnrS2 (colE plasmid) and tetA antibiotic-resistant genes, respectively. A. veronii isolate was highly pathogenic in juvenile Nile tilapia tested in vivo, showing a mortality rate ranging from 3 to 100% in the lowest (1.2 × 104) and highest (1.2 × 108) bacterial dose groups, respectively. To our knowledge, this study would constitute the first report of highly pathogenic and multidrug-resistant A. veronii associated with outbreaks and high mortality rates in tilapia farmed in commercial net cages in Brazil. [ABSTRACT FROM AUTHOR]

Published
2023
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44. Transgenic ‘Hamlin’ sweet orange expressing csd1 or d4e1 genes exhibits decreased susceptibility to citrus canker disease

Author

Docema, Matheus Luís, primary, Moraes, Tatiana de Souza, additional, Attílio, Lísia Borges, additional, Marques, João Paulo Rodrigues, additional, Stipp, Liliane Cristina Liborio, additional, Harakava, Ricardo, additional, Freitas-Astúa, Juliana, additional, Belasque Junior, José, additional, Azevedo, Ricardo Antunes, additional, Gaziola, Salete Aparecida, additional, and Mourão Filho, Francisco de Assis Alves, additional

Published
2022
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45. Impact of native vegetation cover near crops on the occurrence and molecular diversity of fire ants

Author

Nagatani, Victor H., primary, Ramalho, Manuela O., additional, Alves, Juliana M. C., additional, Souza, Rodrigo F., additional, Kayano, Débora Y., additional, Silva, Nathalia S., additional, Silva, Otávio G. M., additional, Harakava, Ricardo, additional, Bueno, Odair C., additional, Hilsdorf, Alexandre W. S., additional, and Morini, Maria S. C., additional

Published
2022
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47. Impact of native vegetation cover near crops on the occurrence and molecular diversity of fire ants.

Author

Nagatani, Victor H., Ramalho, Manuela O., Alves, Juliana M. C., Souza, Rodrigo F., Kayano, Débora Y., Silva, Nathalia S., Silva, Otávio G. M., Harakava, Ricardo, Bueno, Odair C., Hilsdorf, Alexandre W. S., and Morini, Maria S. C.

Subjects
FIRE ants, GROUND vegetation cover, SOLENOPSIS invicta, NATIVE plants, ECOLOGICAL disturbances, HAPLOTYPES
Abstract

Copyright of Agricultural & Forest Entomology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2023
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