Search

Your search keyword '"Harada, Seiko"' showing total 13 results
Start Over Author "Harada, Seiko"
13 results on '"Harada, Seiko"'

1. FZD5 regulates cellular senescence in human mesenchymal stem/stromal cells

Author

Harada, Seiko, primary, Mabuchi, Yo, additional, Kohyama, Jun, additional, Shimojo, Daisuke, additional, Suzuki, Sadafumi, additional, Kawamura, Yoshimi, additional, Araki, Daisuke, additional, Suyama, Takashi, additional, Kajikawa, Masunori, additional, Akazawa, Chihiro, additional, Okano, Hideyuki, additional, and Matsuzaki, Yumi, additional

Published
2020
Full Text
View/download PDF

3. FZD5 regulates cellular senescence in human mesenchymal stem/stromal cells.

Author

Harada, Seiko, Mabuchi, Yo, Kohyama, Jun, Shimojo, Daisuke, Suzuki, Sadafumi, Kawamura, Yoshimi, Araki, Daisuke, Suyama, Takashi, Kajikawa, Masunori, Akazawa, Chihiro, Okano, Hideyuki, and Matsuzaki, Yumi

Subjects
MESENCHYMAL stem cells, GENE expression, AGING, STROMAL cells, TRANSCRIPTION factors
Abstract

Human mesenchymal stem/stromal cells (hMSCs) have garnered enormous interest as a potential resource for cell‐based therapies. However, the molecular mechanisms regulating senescence in hMSCs remain unclear. To elucidate these mechanisms, we performed gene expression profiling to compare clonal immature MSCs exhibiting multipotency with less potent MSCs. We found that the transcription factor Frizzled 5 (FZD5) is expressed specifically in immature hMSCs. The FZD5 cell surface antigen was also highly expressed in the primary MSC fraction (LNGFR+THY‐1+) and cultured MSCs. Treatment of cells with the FZD5 ligand WNT5A promoted their proliferation. Upon FZD5 knockdown, hMSCs exhibited markedly attenuated proliferation and differentiation ability. The observed increase in the levels of senescence markers suggested that FZD5 knockdown promotes cellular senescence by regulating the noncanonical Wnt pathway. Conversely, FZD5 overexpression delayed cell cycle arrest during the continued culture of hMSCs. These results indicated that the intrinsic activation of FZD5 plays an essential role in negatively regulating senescence in hMSCs and suggested that controlling FZD5 signaling offers the potential to regulate hMSC quality and improve the efficacy of cell‐replacement therapies using hMSCs. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

4. FZD5regulates cellular senescence in human mesenchymal stem/stromal cells

Author

Harada, Seiko, Mabuchi, Yo, Kohyama, Jun, Shimojo, Daisuke, Suzuki, Sadafumi, Kawamura, Yoshimi, Araki, Daisuke, Suyama, Takashi, Kajikawa, Masunori, Akazawa, Chihiro, Okano, Hideyuki, and Matsuzaki, Yumi

Abstract

Human mesenchymal stem/stromal cells (hMSCs) have garnered enormous interest as a potential resource for cell‐based therapies. However, the molecular mechanisms regulating senescence in hMSCs remain unclear. To elucidate these mechanisms, we performed gene expression profiling to compare clonal immature MSCs exhibiting multipotency with less potent MSCs. We found that the transcription factor Frizzled 5 (FZD5) is expressed specifically in immature hMSCs. The FZD5 cell surface antigen was also highly expressed in the primary MSC fraction (LNGFR+THY‐1+) and cultured MSCs. Treatment of cells with the FZD5 ligand WNT5A promoted their proliferation. Upon FZD5knockdown, hMSCs exhibited markedly attenuated proliferation and differentiation ability. The observed increase in the levels of senescence markers suggested that FZD5knockdown promotes cellular senescence by regulating the noncanonical Wnt pathway. Conversely, FZD5overexpression delayed cell cycle arrest during the continued culture of hMSCs. These results indicated that the intrinsic activation of FZD5plays an essential role in negatively regulating senescence in hMSCs and suggested that controlling FZD5 signaling offers the potential to regulate hMSC quality and improve the efficacy of cell‐replacement therapies using hMSCs. Although mesenchymal stem/stromal cells (MSCs) are promising for cell therapy, long‐term culture leads to MSC senescence with reduced stem cell capacity. We showed that FZD5, which is specifically expressed in immature MSCs, can serve as a stemness indicator. Controlling FZD5 signaling may improve MSC quality and the outcomes of cell therapy.

Published
2021
Full Text
View/download PDF

12. Hemodialysis for an AIDS patient

Author

Kawamoto, Sachiko, primary, Tsutsui, Kikue, additional, Chihara, Itsuko, additional, Hirai, Tomiko, additional, Makino, Taiko, additional, and Harada, Seiko, additional

Published
1989
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results