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381 results on '"Harada, Hidemitsu"'

7. Characterization of Dental Epithelial Stem Cells from the Mouse Incisor with Two-Dimensional and Three-Dimensional Platforms

Author

Chavez, Miquella G, Yu, Wenli, Biehs, Brian, Harada, Hidemitsu, Snead, Malcolm L, Lee, Janice S, Desai, Tejal A, and Klein, Ophir D

Subjects
Engineering, Biomedical Engineering, Dental/Oral and Craniofacial Disease, Stem Cell Research - Nonembryonic - Non-Human, Stem Cell Research, Regenerative Medicine, Animals, Cell Adhesion, Cell Culture Techniques, Cell Proliferation, Cell-Matrix Junctions, Cells, Cultured, Epithelial Cells, Incisor, Intercellular Junctions, Mice, Mice, Inbred C57BL, Spheroids, Cellular, Stem Cells, Biochemistry and Cell Biology, Biomedical engineering
Abstract

Dental epithelial stem cells (DESCs) drive continuous growth in the adult mouse incisors. To date, a robust system for the primary culture of these cells has not been reported, and little is known about the basic molecular architecture of these cells or the minimal extracellular scaffolding that is necessary to maintain the epithelial stem cell population in an undifferentiated state. We report a method of isolating DESCs from the cervical loop of the mouse mandibular incisor. Cells were viable in a two-dimensional culture system and did not demonstrate preferential proliferation when grown on top of various substrates. Characterization of these cells indicated that E-cadherin, integrin alpha-6, and integrin beta-4 mark the DESCs both in vivo and in vitro. We also grew these cells in a three-dimensional microenvironment and obtained spheres with an epithelial morphology and expression patterns. Insights into the mechanisms of stem cell maintenance in vitro will help lay the groundwork for the successful generation of bioengineered teeth from adult DESCs.

Published
2013

30. Inhibition of L‐type voltage‐gated calcium channel‐mediated Ca2+ influx suppresses the collective migration and invasion of ameloblastoma.

Author

Li, Shujin, Kim, Hyun‐Yi, Lee, Dong‐Joon, Park, Sung‐Ho, Otsu, Keishi, Harada, Hidemitsu, Jung, Young‐Soo, and Jung, Han‐Sung

Subjects
AMELOBLASTOMA, CELL aggregation, CALCIUM, CALCIUM channels, NUCLEOTIDE sequencing, CELL migration inhibition, CANCER cells
Abstract

Objectives: Ameloblastoma (AM) has been known as a benign but locally invasive tumour with high recurrence rates. Invasive behaviour of the AM results in destruction of the adjacent jawbone and the non‐detectable remnants during surgery, interrupting the complete elimination of cancer cells. Methods: To explore novel targets for the tumour cell invasion, a transcriptomic analysis between AM and odontogenic keratocyst were performed through next‐generation sequencing in detail. Results: Enrichment of CACNA1C gene (encoding Cav1.2) in AM, a subunit of the L‐type voltage‐gated calcium channel (VGCC) was observed for the first time. The expression and channel activity of Cav1.2 was confirmed by immunostaining and calcium imaging in the patient samples or primary cells. Verapamil, L‐type VGCC blocker revealed suppression of the Ca2+‐induced cell aggregation and collective invasion of AM cells in vitro. Furthermore, the effect of verapamil in suppressing AM invasion into the adjacent bone was confirmed through orthotopic xenograft model specifically. Conclusion: Taken together, Cav1.2 maybe considered to be a therapeutic candidate to decrease the collective migration and invasion of AM. [ABSTRACT FROM AUTHOR]

Published
2022
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32. Local application of Usag-1 siRNA can promote tooth regeneration in Runx2-deficient mice

Author

50211371, 90229138, Mishima, Sayaka, Takahashi, Katsu, Kiso, Honoka, Murashima-Suginami, Akiko, Tokita, Yoshihito, Jo, Jun-Ichiro, Uozumi, Ryuji, Nambu, Yukiko, Huang, Boyen, Harada, Hidemitsu, Komori, Toshihisa, Sugai, Manabu, Tabata, Yasuhiko, Bessho, Kazuhisa, 50211371, 90229138, Mishima, Sayaka, Takahashi, Katsu, Kiso, Honoka, Murashima-Suginami, Akiko, Tokita, Yoshihito, Jo, Jun-Ichiro, Uozumi, Ryuji, Nambu, Yukiko, Huang, Boyen, Harada, Hidemitsu, Komori, Toshihisa, Sugai, Manabu, Tabata, Yasuhiko, and Bessho, Kazuhisa

Abstract

Runt-related transcription factor 2 (Runx2)-deficient mice can be used to model congenital tooth agenesis in humans. Conversely, uterine sensitization-associated gene-1 (Usag-1)-deficient mice exhibit supernumerary tooth formation. Arrested tooth formation can be restored by crossing both knockout-mouse strains; however, it remains unclear whether topical inhibition of Usag-1 expression can enable the recovery of tooth formation in Runx2-deficient mice. Here, we tested whether inhibiting the topical expression of Usag-1 can reverse arrested tooth formation after Runx2 abrogation. The results showed that local application of Usag-1 Stealth small interfering RNA (siRNA) promoted tooth development following Runx2 siRNA-induced agenesis. Additionally, renal capsule transplantation of siRNA-loaded cationized, gelatin-treated mouse mandibles confirmed that cationized gelatin can serve as an effective drug-delivery system. We then performed renal capsule transplantation of wild-type and Runx2-knockout (KO) mouse mandibles, treated with Usag-1 siRNA, revealing that hindered tooth formation was rescued by Usag-1 knockdown. Furthermore, topically applied Usag-1 siRNA partially rescued arrested tooth development in Runx2-KO mice, demonstrating its potential for regenerating teeth in Runx2-deficient mice. Our findings have implications for developing topical treatments for congenital tooth agenesis.

Published
2021

33. A novel cyclic peptide (Naturido) modulates glia–neuron interactions in vitro and reverses ageing-related deficits in senescence-accelerated mice

Author

ISHIGURO, Shinichi, SHINADA, Tetsuro, WU, Zhou, KARIMAZAWA, Mayumi, UCHIDATE, Michimasa, NISHIMURA, Eiji, YASUNO, Yoko, EBATA, Makiko, SILLAPAKONG, Piyamas, ISHIGURO, Hiromi, EBATA, Nobuyoshi, NI, Junjun, JIANG, Muzhou, GORYO, Masanobu, OTSU, Keishi, HARADA, Hidemitsu, SUZUKI, Koichi, ISHIGURO, Shinichi, SHINADA, Tetsuro, WU, Zhou, KARIMAZAWA, Mayumi, UCHIDATE, Michimasa, NISHIMURA, Eiji, YASUNO, Yoko, EBATA, Makiko, SILLAPAKONG, Piyamas, ISHIGURO, Hiromi, EBATA, Nobuyoshi, NI, Junjun, JIANG, Muzhou, GORYO, Masanobu, OTSU, Keishi, HARADA, Hidemitsu, and SUZUKI, Koichi

Abstract

The use of agents that target both glia and neurons may represent a new strategy for the treatment of ageing disorders. Here, we confirmed the presence of the novel cyclic peptide Naturido that originates from a medicinal fungus (Isaria japonica) grown on domestic silkworm (Bombyx mori). We found that Naturido significantly enhanced astrocyte proliferation and activated the single copy gene encoding the neuropeptide VGF and the neuron-derived NGF gene. The addition of the peptide to the culture medium of primary hippocampal neurons increased dendrite length, dendrite number and axon length. Furthermore, the addition of the peptide to primary microglial cultures shifted CGA-activated microglia towards anti-inflammatory and neuroprotective phenotypes. These findings of in vitro glia–neuron interactions led us to evaluate the effects of oral administration of the peptide on brain function and hair ageing in senescence-accelerated mice (SAMP8). In vivo analyses revealed that spatial learning ability and hair quality were improved in Naturido-treated mice compared with untreated mice, to the same level observed in the normal ageing control (SAMR1). These data suggest that Naturido may be a promising glia–neuron modulator for the treatment of not only senescence, but also Alzheimer’s disease and other neurodegenerative diseases.

Published
2021

40. A novel cyclic peptide (Naturido) modulates glia–neuron interactions in vitro and reverses ageing-related deficits in senescence-accelerated mice

Author

Ishiguro, Shinichi, primary, Shinada, Tetsuro, additional, Wu, Zhou, additional, Karimazawa, Mayumi, additional, Uchidate, Michimasa, additional, Nishimura, Eiji, additional, Yasuno, Yoko, additional, Ebata, Makiko, additional, Sillapakong, Piyamas, additional, Ishiguro, Hiromi, additional, Ebata, Nobuyoshi, additional, Ni, Junjun, additional, Jiang, Muzhou, additional, Goryo, Masanobu, additional, Otsu, Keishi, additional, Harada, Hidemitsu, additional, and Suzuki, Koichi, additional

Published
2021
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