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Enterobacteriaceae that produce extended‐spectrum β‐lactamases (ESBLs) can result in severe human infections, contributing to the development of complex diseases. Klebsiella pneumoniae is one of the ESBL‐producing pathogens that helps to set antimicrobial resistance as a major public health problem worldwide. The current study aimed to isolate, identify, and characterize ESBL‐producing K. pneumoniae and their antimicrobial resistance pattern in retail cattle meat samples. A comprehensive set of 225 cattle meat samples was gathered from 13 upazilas within the Sylhet district of Bangladesh. The bacterial isolates were obtained through biochemical and cultural techniques, and the identification of K. pneumoniae was accomplished using polymerase chain reactions (PCRs). Antimicrobial susceptibilities were assessed using disk diffusion in accordance with the Clinical and Laboratory Standards Institute (CLSI, 2020) guidelines. Genes encoding ESBL enzymes were detected by the double‐disk synergy test (DDST) and multiplex PCR. The overall prevalence of Klebsiella spp. was 28.89% (65/225), whereas the positive percentage of K. pneumoniae was 59.2% (29/49) confirmed by PCR. Antimicrobial resistance was observed against 12 antibiotics. According to the phenotypic resistance pattern determined through the disk diffusion method, all isolates (100%) were resistant to ampicillin, amoxicillin, cefuroxime, cefotaxime, and colistin. On the other hand, the highest susceptibility was observed towards gentamicin (97.95%), followed by ciprofloxacin (85.71%), tetracycline (83.67%), and trimethoprim‐sulfamethoxazole (81.63%). Out of the total K. pneumoniae isolates analyzed, ESBL genes were present, and the highest percentage, 82.8% (24/29), tested positive for blaTEM genes. Interestingly, among the nine ESBL genes, six were identified in K. pneumoniae isolates, except for blaOXA, blaCTX‐M‐grp2, and MultiCaseDHA. The study's results reveal the presence of extended‐spectrum β‐lactamase (ESBL)‐producing multidrug‐resistant (MDR) K. pneumoniae in retail cattle meat samples posing a substantial public health threat. [ABSTRACT FROM AUTHOR]

Key Clinical Message: Toxic Epidermal Necrolysis (TEN) is a rare, but potentially fatal mucocutaneous reaction, that may occur due to an immunologic response to certain medications. However, TEN triggered by Trastuzumab is extremely rare. Early diagnosis, recognition, and prompt cessation of the offending drugs and initiation of steroid therapy with supportive management are the most important actions for managing TEN. Although rare, it is important to be vigilant about this potential adverse reactions associated with trastuzumab to ensure patient safety and contribute to better outcomes. [ABSTRACT FROM AUTHOR]

The Pasur-Sibsa and Baleshwari River Estuary (PSBE) is the longest estuary located in the southwest part of Bangladesh on the Ganges-Brahmaputra delta which flows into the Bay of Bengal. The study was conducted to investigate the diversity and abundance of the zooplankton from three gateways (S1 station: Pasur river gateway; S2 station: Sibsa river gateway and S3 station: Baleshwari river gateway) in the PSBE from July 2022 to December 2022. Water samples were collected from these gateways using plankton net (55 µm mesh size) and preserved for analyzing in the laboratory. A total of 34 species of zooplankton belonging to 9 orders and 17 families were documented. The dominant orders were Calanoida and Dendrobranchiata, covering 59.52% and 18.20%, respectively. Acartia bilobata was the dominant species, while Sagitta sp. was the inferior species throughout the gateways. The Pasur river estuary gateway showed the highest percentage of abundance, accounting for 36.39% of the total individuals. The study calculated the Shannon-Wiener Diversity Index, Simpson's Dominance Index, Simpson's Index of Diversity, Margalef's Richness Index, and Evenness ranged from 1.33 to 1.43, 0.24 to 0.30, 0.70-0.76, 1.11 to 1.21, and 0.74-0.80, respectively. The results showed that the zooplankton composition was rich in the S1 station, which serves as a good indicator of marine productivity like zooplankton. The density of zooplankton in a body of water is indicative of the fish stocking rate. [ABSTRACT FROM AUTHOR]