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26 results on '"Hapairai, Limb K."'

3. Studies on Aedes polynesiensis introgression and ecology to facilitate lymphatic filariasis control

Author

Hapairai, Limb K. M. and Sinkins, Steven P.

Subjects
614.4, Zoological sciences, Disease (zoology), Ecology (zoology), Aedes polynesiensis, lymphatic filariasis, Wolbachia, sterile insect technique
Abstract

The mosquito Aedes polynesiensis, a member of the Aedes scutellaris complex, is the main vector in the South Pacific region of the Wuchereria bancrofti parasite, the causative agent of lymphatic filariasis (LF), and is also a major nuisance biter. Decades of Mass Drug treatment (MDA) have not been successful in elimination LF. Two non-vector species in the Ae. scutellaris complex were introgressed with Ae. polynesiensis to attempt to obtain lines that would produce cytoplasmic incompatibility (CI) with wild populations and/or LF-refractoriness. Despite selection of progeny from Brugia-challenged, non-infective females at each backcross, no refractory line was acquired. However, three lines from crosses between aposymbiotic Ae. polynesiensis and Ae. riversi displayed CI and male mating competiveness suitable for the purpose of population suppression using the incompatible insect technique (IIT). A population study was conducted of potential release sites and the evaluation of monitoring tools for Ae. polynesiensis on Moorea and Tetiaroa, French Polynesia. There was no evidence of active migration between selected islets on the atoll of Tetiaroa, suggesting it is a suitable site for field releases of CI males. The BioGents Sentinel trap was shown to be an efficient and convenient trap suitable for Ae. polynesiensis monitoring. The effects of temperature and larval density on life-table parameters relevant to IIT were examined, including: larval survivorship, developmental time to pupation, male to female ratio, male pupae yield, male size and adult male survival. These findings were used to design and conduct a 14-week field experiment testing CI male strain against an isolated population, using optimized rearing conditions. Approximately 8000 males were released weekly on motu Onetahi, Tetiaroa atoll. Significant sterility was induced by Wolbachia in the targeted female population, supporting the development and scale-up of this approach toward Ae. polynesiensis nuisance and LF transmission reduction.

Published
2013

6. Correction: Mysore et al. A Broad-Based Mosquito Yeast Interfering RNA Pesticide Targeting Rbfox1 Represses Notch Signaling and Kills Both Larvae and Adult Mosquitoes. Pathogens 2021, 10, 1251

Author

Mysore, Keshava, primary, Sun, Longhua, additional, Hapairai, Limb K., additional, Wang, Chien-Wei, additional, Roethele, Joseph B., additional, Igiede, Jessica, additional, Scheel, Max P., additional, Scheel, Nicholas D., additional, Li, Ping, additional, Wei, Na, additional, Severson, David W., additional, and Duman-Scheel, Molly, additional

Published
2022
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9. A Yeast RNA-Interference Pesticide Targeting the Irx Gene Functions as a Broad-Based Mosquito Larvicide and Adulticide

Author

Mysore, Keshava, primary, Sun, Longhua, additional, Hapairai, Limb K., additional, Wang, Chien-Wei, additional, Igiede, Jessica, additional, Roethele, Joseph B., additional, Scheel, Nicholas D., additional, Scheel, Max P., additional, Li, Ping, additional, Wei, Na, additional, Severson, David W., additional, and Duman-Scheel, Molly, additional

Published
2021
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10. A Broad-Based Mosquito Yeast Interfering RNA Pesticide Targeting Rbfox1 Represses Notch Signaling and Kills Both Larvae and Adult Mosquitoes

Author

Mysore, Keshava, primary, Sun, Longhua, additional, Hapairai, Limb K., additional, Wang, Chien-Wei, additional, Roethele, Joseph B., additional, Igiede, Jessica, additional, Scheel, Max P., additional, Scheel, Nicholas D., additional, Li, Ping, additional, Wei, Na, additional, Severson, David W., additional, and Duman-Scheel, Molly, additional

Published
2021
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13. Characterization of a dual-action adulticidal and larvicidal interfering RNA pesticide targeting the Shaker gene of multiple disease vector mosquitoes

Author

Mysore, Keshava, primary, Hapairai, Limb K., additional, Sun, Longhua, additional, Li, Ping, additional, Wang, Chien-Wei, additional, Scheel, Nicholas D., additional, Lesnik, Alexandra, additional, Igiede, Jessica, additional, Scheel, Max P., additional, Wei, Na, additional, Severson, David W., additional, and Duman-Scheel, Molly, additional

Published
2020
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14. Characterization of a yeast interfering RNA larvicide with a target site conserved in the synaptotagmin gene of multiple disease vector mosquitoes

Author

Mysore, Keshava, primary, Li, Ping, additional, Wang, Chien-Wei, additional, Hapairai, Limb K., additional, Scheel, Nicholas D., additional, Realey, Jacob S., additional, Sun, Longhua, additional, Roethele, Joseph B., additional, Severson, David W., additional, Wei, Na, additional, and Duman-Scheel, Molly, additional

Published
2019
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18. Evaluation of traps and lures for mosquito vectors and xenomonitoring of Wuchereria bancrofti infection in a high prevalence Samoan Village

Author

Hapairai, Limb K, primary, Plichart, Catherine, additional, Naseri, Take, additional, Silva, Ualesi, additional, Tesimale, Lameko, additional, Pemita, Paulo, additional, Bossin, Hervé C, additional, Burkot, Thomas R, additional, Ritchie, Scott A, additional, Graves, Patricia M, additional, Melrose, Wayne, additional, and Joseph, Hayley, additional

Published
2015
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23. Comparison of the Centers for Disease Control and Prevention-Backpack and Insectazooka Aspirators for Sampling Aedes polynesiensis in French Polynesia.

Author

Hapairai, Limb K., Cheong Sang, Michel A., and Bossin, Hervé C.

Abstract

The efficiency of the recently developed handheld InsectaZooka™ (IZ) aspirator was compared to that of the Centers for Disease Control and Prevention-Backpack (CDC-BP) aspirator by conducting human bait collections on 2 islets (locally called motus) of the atoll of Tetiaroa, French Polynesia. Abundance of mosquitoes was compared between the wind-exposed and wind-protected sides of each motu to measure the effect of wind on mosquito distribution. The number of host-seeking Aedes polynesiensis mosquitoes collected on the 2 motus with either sampling device was not significantly different. Collection of male mosquitoes was low irrespective of the type of aspirator used. Wind had an effect on mosquito distribution, as females were more abundant on the protected sides of both motus. The IZ aspirator is a lighter and equally efficient alternative to the CDC-BP aspirator for collecting Ae. polynesiensis. [ABSTRACT FROM AUTHOR]

Published
2014
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24. Characterization of an adulticidal and larvicidal interfering RNA pesticide that targets a conserved sequence in mosquito G protein-coupled dopamine 1 receptor genes.

Author

Hapairai, Limb K., Mysore, Keshava, Sun, Longhua, Li, Ping, Wang, Chien-Wei, Scheel, Nicholas D., Lesnik, Alexandra, Scheel, Max P., Igiede, Jessica, Wei, Na, Severson, David W., and Duman-Scheel, Molly

Subjects
*DOPAMINE receptors, *MOSQUITO control, *SMALL interfering RNA, *PESTICIDES, *AEDES aegypti, *MOSQUITOES, *AEDES albopictus, *G protein coupled receptors
Abstract

G protein-coupled receptors (GPCRs), key regulators of a variety of critical biological processes, are attractive targets for insecticide development. Given the importance of these receptors in many organisms, including humans, it is critical that novel pesticides directed against GPCRs are designed to be species-specific. Here, we present characterization of an interfering RNA pesticide (IRP) targeting the mosquito GPCR-encoding dopamine 1 receptor (dop1) genes. A small interfering RNA corresponding to dop1 was identified in a screen for IRPs that kill Aedes aegypti during both the adult and larval stages. The 25 bp sequence targeted by this IRP is conserved in the dop1 genes of multiple mosquito species, but not in non-target organisms, indicating that it could function as a biorational mosquito insecticide. Aedes aegypti adults treated through microinjection or attractive toxic sugar bait delivery of small interfering RNA corresponding to the target site exhibited severe neural and behavioral defects and high levels of adult mortality. Likewise, A. aegypti larval consumption of dried inactivated yeast tablets prepared from a Saccharomyces cerevisiae strain engineered to express short hairpin RNA corresponding to the dop1 target site resulted in severe neural defects and larval mortality. Aedes albopictus and Anopheles gambiae adult and larval mortality was also observed following treatment with dop1 IRPs, which were not toxic to non-target arthropods. The results of this investigation indicate that dop1 IRPs can be used for species-specific targeting of dop1 GPCRs and may represent a new biorational strategy for control of both adult and larval mosquitoes. Image 1 • RNAi pesticides targeting mosquito dopamine 1 receptor (dop1) genes kill adults and larvae. • RNAi pesticides targeting dop1 can be delivered to adults as attractive toxic sugar baits. • Yeast that express RNAi pesticides targeting dop1 kill mosquito larvae upon consumption. • Interfering RNA pesticides can be used for species-specific targeting of mosquito dop1 GPCRs. • RNAi-based pesticides targeting dop1 may represent a new biorational mosquito control strategy. [ABSTRACT FROM AUTHOR]

Published
2020
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26. Preparation and Use of a Yeast shRNA Delivery System for Gene Silencing in Mosquito Larvae.

Author

Mysore K, Hapairai LK, Wei N, Realey JS, Scheel ND, Severson DW, and Duman-Scheel M

Subjects
Animals, Larva, Malaria parasitology, Mosquito Control, Mosquito Vectors genetics, Protozoan Proteins genetics, RNA, Small Interfering genetics, Anopheles genetics, Drug Delivery Systems, Gene Silencing, Malaria prevention & control, Protozoan Proteins antagonists & inhibitors, RNA, Small Interfering administration & dosage, Saccharomyces cerevisiae
Abstract

The mosquito genome projects facilitated research in new facets of mosquito biology, including functional genetic studies in the dengue and Zika virus vector Aedes aegypti and the primary African malaria vector Anopheles gambiae. RNA interference (RNAi) has facilitated gene silencing experiments in both of these disease vector mosquito species and could one day be applied as a new method of vector control. Here, we describe a procedure for the genetic engineering of Saccharomyces cerevisiae (baker's yeast) that express short hairpin RNA (shRNA) corresponding to mosquito target genes of interest. Following cultivation, which facilitates inexpensive propagation of shRNA, the yeast is inactivated and prepared in a ready-to-use dry tablet formulation that is fed to mosquito larvae. Ingestion of the yeast tablets results in effective larval target gene silencing. This technically straightforward and affordable technique may be applicable to a wide variety of mosquito species and potentially to other arthropods that feed on yeast.

Published
2019
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