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Solid pseudopapillary neoplasm (SPN) of the pancreas is a rare neoplasm that mainly affects young women.To evaluate the impact of parenchyma-preserving surgical methods (PPMs, including enucleation and central pancreatectomy) in the treatment of SPN patients.From 2013 to 2019, patients who underwent pancreatectomy for SPNs were retrospectively reviewed. The baseline characteristics, intraoperative index, pathological outcomes, short-term complications and long-term follow-up data were compared between the PPM group and the conventional method (CM) group.In total, 166 patients were included in this study. Of them, 33 patients (19.9%) underwent PPM. Most of the tumors (104/166, 62.7%) were found accidentally. Comparing the parameters between groups, the hospital stay d (12.35PPM for SPN appears to be feasible and safe for preserving the exocrine function of the pancreas.

An anaerobic bacterial strain, designated as NSJ-90T, was isolated from the faeces of a healthy adult in China. Cells of strain NSJ-90T were Gram-stain-negative, non-motile, non-spore-forming and rod-shaped. Based on 16S rRNA gene sequence analysis, strain NSJ-90T belonged to the genus Bacteroides and was phylogenetically closely related to Bacteroides clarus YIT 12056T (16S rRNA gene identity was 97.04 %). The DNA G+C content of strain NSJ-90T was 44.85 mol% (calculated from the genome). The average nucleotide identity between strain NSJ-90T and B. clarus YIT 12056T was 87.60 %. The major cellular fatty acids (>10 %) of strain NSJ-90T were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0 3-OH. Menaquinone-10 was detected as the respiratory quinone. The major products of glucose fermentation were acetic, propionic and isovaleric acids. Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, we propose that strain NSJ-90T represents a novel species of the genus Bacteroides , for which the name Bacteroides propionicigenes sp. nov. is proposed. The type strain is NSJ-90T (=CGMCC 1.17886T=KCTC 25305T).

Biological foaming (or biofoaming) is a frequently occurring problem in wastewater treatment plants (WWTPs) and is attributed to the overwhelming growth of filamentous bulking and foaming bacteria (BFB). Biological foaming has been intensively investigated, with BFB like Microthrix and Skermania having been identified from WWTPs and implicated in foaming. Nevertheless, studies are still needed to improve our understanding of the microbial diversity of WWTP biofoams and how microbial activities contribute to foaming. In this study, sludge foaming at the Qinghe WWTP of China was monitored, and sludge foams were investigated using culture-dependent and culture-independent microbiological methods. The foam microbiomes exhibited high abundances of Skermania, Mycobacterium, Flavobacteriales, and Kaistella. A previously unknown bacterium, Candidatus Kaistella beijingensis, was cultivated from foams, its genome was sequenced, and it was phenotypically characterized. Ca. K. beijingensis exhibits hydrophobic cell surfaces, produces extracellular polymeric substances (EPS), and metabolizes lipids. Ca. K. beijingensis abundances were proportional to EPS levels in foams. Several proteins encoded by the Ca. K. beijingensis genome were identified from EPS that was extracted from sludge foams. Ca. K. beijingensis populations accounted for 4 to 6% of the total bacterial populations in sludge foam samples within the Qinghe WWTP, although their abundances were higher in spring than in other seasons. Cooccurrence analysis indicated that Ca. K. beijingensis was not a core node among the WWTP community network, but its abundances were negatively correlated with those of the well-studied BFB Skermania piniformis among cross-season Qinghe WWTP communities. IMPORTANCE Biological foaming, also known as scumming, is a sludge separation problem that has become the subject of major concern for long-term stable activated sludge operation in decades. Biological foaming was considered induced by foaming bacteria. However, the occurrence and deterioration of foaming in many WWTPs are still not completely understood. Cultivation and characterization of the enriched bacteria in foaming are critical to understand their genetic, physiological, phylogenetic, and ecological traits, as well as to improve the understanding of their relationships with foaming and performance of WWTPs.

To improve the corrosion resistance, surface electrical conductivity and wettability of Ti-6Al-4V used in polymer electrolyte membrane fuel cell (PEMFC), a ZrCN nanocrystalline coating was deposited on Ti-6Al-4V substrate using double cathode glow discharge technique. The new coating exhibited a nanocomposite structure, consisting of amorphous C, CNx and nanocrystalline ZrCN. The effect of the HF concentrations on the corrosion behavior of the coating was investigated by potentiodynamic, potentiostatic polarizations and electrochemical impedance spectroscopy (EIS) in a simulated the operating conditions of a PEMFC. With increasing HF concentrations, the corrosion potential (E-corr) decreased and the corrosion current density (i(corr)) of the ZrCN coating increased, indicating that corrosion resistance decreased with the increase of HF concentrations. However, at any given concentration of HF, the corrosion resistance of the ZrCN coating was significantly higher than that of uncoated Ti-6Al-4V. The results of EIS measurements showed that with increasing the concentration of HF, the resistance of the passive film (R-b) formed on the ZrCN coating decreased slightly, being of the order of magnitude of similar to 10(7) Omega cm(2), which was an improvement by four orders of magnitude compared to uncoated Ti-6Al-4V. At a compaction force of 140 N cm(-2), no perceptible difference in the interfacial contact resistance (ICR) of ZrCN-coated Ti-6Al-4V was observed before and after potentiostatic polarization for 120 min, and its ICR values were reduced by one order of magnitude in comparison to that of uncoated Ti-6Al-4V. Moreover, ZrCN-coated Ti-6Al-4V exhibited a much low surface wettability than uncoated Ti-6Al-4V alloy, which was beneficial for both water management and improving corrosion resistance. (C) 2015 Elsevier B.V. All rights reserved.

Tumor suppressor gene silencing via promoter hypermethylation is an important event in pancreatic cancer pathogenesis. Aberrant DNA hypermethylation events are highly tumor specific, and may provide a diagnostic tool for pancreatic cancer patients. The objective of the current study was to identify novel methylation-related genes that may potentially be used to establish novel therapeutic and diagnostic strategies against pancreatic cancer. The methylation status of the GS homeobox 2 (GSH2) gene was analyzed using the sodium bisulfite sequencing method. The GSH2 methylation ratio was examined in primary carcinomas and corresponding normal tissues derived from 47 patients with pancreatic cancer, using quantitative methylation-specific polymerase chain reaction. Methylation ratios were found to be associated with the patient's clinicopathological features. GSH2 gene methylation was detected in 26 (55.3%) of the 47 pancreatic cancer patients, indicating that it occurs frequently in pancreatic cancer. A significant association with methylation was observed for tumor-node-metastasis stage (P=0.031). GSH2 may be a novel methylation-sensitive tumor suppressor gene in pancreatic cancer and may be a tumor-specific biomarker of the disease.

Based on the discrete element method, the three dimensional mixed-size grain/bed collision process has been investigated in this paper. By considering the influence of lateral impact angle on the mean ejection velocity and ejection number, we obtain that the mean lateral ejection velocity increases with the lateral impact angle at large mean diameter cases and also increases with the impact velocity, while decreases with impact angle. The simulation results indicate that although the lateral impact affects the mean ejection velocity, its effect on the mean ejection number is very limited. Moreover, the mean lateral ejection velocity is influenced by the impact angular velocity. Based on these studies, a more comprehensive three dimensional mixed-size splash function is given. It is of great significance to the particle motion based on turbulence in aeolian sand landform.

Recent observation results show that sand ripples and dunes are movable like those on Earth under current Martian climate. And the aeolian process on Mars therefore is re-attracting the eyes of scientific researchers in different fields. In this paper, the spatial and temporal evolution of wind-blown sand on Mars is simulated by the large-eddy simulation method. The simulations are conducted under the conditions of both friction wind speed higher and lower than the "fluid threshold", respectively. The fluid entrainment of the sand particles, the processes among saltation sand particles and sand bed, and the negative feedback of sand movement to flow field are considered. Our results show that the "overshoot" phenomenon also exists in the evolution of wind-blown sand on Mars both temporally and spatially; impact entrainment affects the sand transport rate on Mars when the wind speed is smaller or larger than the fluid threshold; and both the average saltation length and height are one order of magnitudes larger than those on Earth. Eventually, the formulas describing the sand transport rate, average saltation length and height on Mars are given, respectively.

The full length cDNA of human B lymphocyte stimulator (hBLyS) was amplified by using PCR method from cDNA library of human placenta. After purifying and sequencing, the DNA fragment of functional domain of hBLyS (hsDNA fragment)was amplified by using nested PCR method from the PCR product. The prokaryotic expression plasmid pET-30a( )/ hBLyS was constructed with recombinant DNA techniques after purifying and identifying the hsDNA fragment. Then the plasmid pET-30a( )/ hBLyS was transformed into lambdaDE3 cells and the recombination protein was found to be highly expressed the expression product was purified by affinity chromatography gel, Ni(2 )-IDA, made in our laboratory. The experimental results showed that the sequence of the PCR product was identical with the published hBLyS cDNA sequence and purity of the recombination protein we obtained was high. The activity of the purified recombination protein was very significant in the proliferation test of B lymphocytes.