7 results on '"Hao, Yan-qin"'
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2. Identification and treatment of a case of Taenia saginata infection in an adult.
- Author
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WANG Jin-fen and HAO Yan-qin
- Abstract
Through the reports of the identification and treatment of a case of Taenia Saginata infection in an adult, we provide experience for the diagnosis and treatment of Taeniasis. Methods The patient was young woman, self-employed, and had a history of eating raw beef. She had intermittent abdominal pain for half a year, and white strip worms were found to have defecated. Species identification was carried out according to the morphological characteristics of the segments. According to the worm body by naked eyes, the part of worm body and ovum morphology under microscope, the diagnosis was made based on clinical manifestations and combined with the history of epidemiology. Results The parasite excreted by the patient was diagnosed with Taenia Saginata infection by morphological identification. Traditional Chinese medicine areca and pumpkin seeds were used to carry out deworming treatment, and the treatment was successful. The patient was not reinfected through follow up. Conclusion People are mostly infected with Taeniasis by eating beef containing live cysticercus, and people are generally susceptible. With the improvement of environmental, Taeniasis is rarely. People still have the chance of infected Taenia Saginata if the diet is unclean, raw and cooked food is not divided. It is not limited to relevant personnel in pastoral areas. Therefore, people need to be vigilant and develop healthy eating habits. Relevant personnel should strengthen health publicity and education, meat quarantine, and actively prevent. Once suspected tapeworm infection is found, timely visit the doctor for thorough treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
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3. 基于信息化的医院科研绩效考核的实施策略*
- Author
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Hao, Yan-qin, primary and Wu, Hui-qin, additional
- Published
- 2015
- Full Text
- View/download PDF
4. [Changes in mast cells and hepatic expression of c-kit and stem cell factor in the rat model of chronic hepatitis].
- Author
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Li H, Zhao LF, Hao YQ, Yin L, Zhao YC, and Han DW
- Subjects
- Animals, Cell Degranulation, Disease Models, Animal, Female, Hepatitis, Chronic pathology, Hepatocytes metabolism, Liver metabolism, Liver Cirrhosis metabolism, Liver Cirrhosis pathology, Male, Mast Cells physiology, RNA, Messenger genetics, Rats, Rats, Wistar, Hepatitis, Chronic metabolism, Mast Cells metabolism, Proto-Oncogene Proteins c-kit metabolism, Stem Cell Factor metabolism
- Abstract
Objective: To study the potential role of mast cells and the related molecular mechanism in chronic hepatitis (CH) using a rat model system., Methods: Thirty Wistar rats (15 males, 15 females; weight range: 230-290 g) were randomly divided into the normal contrast (NC) group and experimental CH group. The CH group received subcutaneous injection of CCl4 and a diet high in cholesterol and alcohol content and low in protein and choline content. Throughout the 4-week modeling period, aseptic blood samples were taken to test plasma tryptase (TS) and hyaluronic acid (HA) levels. The rats were euthanized to assess the changes in liver mast cells by histology and morphology analyses and the changes in liver expression of c-kit and stem cell factor (SCF) proteins by immunohistochemistry and mRNAs by RT-PCR., Results: Compared to the NC group, the CH group had higher plasma and liver concentration of HA (78.09 +/- 38.55 vs. 145.14 +/- 52.54 ng/ml, 51.58 +/- 20.45 vs. 106.59 +/- 43.15 ng/100 mg; t = 2.457 and 2.825 respectively, both P less than 0.05) and TS (0.416 +/- 0.143 vs 0.753 +/- 0.210 mg/ml; t = 4.165, P less than 0.05). The CH group also showed fatty degeneration and fibrosis with many degranulating and degranulated mast cells filled with purple granula located around the liver blood vessels and in fiber-intervals. The CH livers also showed a significantly higher number of mast cells (2.167 +/- 0.924 vs. NC: 10.92 +/- 1.575; t = 7.633, P less than 0.05) and stronger intensity of c-kit staining (2.783 +/- 0.577 vs. 12.86 +/- 3.126; t = 9.511, P less than 0.05) and SCF staining (3.383 +/- 1.583 vs. 15.58 +/- 6.431; t = 9.625, P less than 0.05). The expressions of c-kit and SCF were positively correlated with HA level (r = 0.478 and 0.556 respectively, both P less than 0.05). The c-kit and SCF mRNA expression levels were also significantly higher in the CH liver tissues., Conclusion: Mast cell degranulation and histamine release is significantly increased under conditions of chronic hepatitis, and the related mechanism may involve up-regulation of the membrane receptor c-kit and its ligand SCF.
- Published
- 2013
- Full Text
- View/download PDF
5. [Effects of antihistamine treatment on immune function in rats with experimental hepatitis].
- Author
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Li H, Zhao LF, Hao YQ, and Han DW
- Subjects
- Animals, Ascorbic Acid pharmacology, Disease Models, Animal, Hepatitis immunology, Interferon-gamma metabolism, Interleukin-10 metabolism, Interleukin-12 metabolism, Interleukin-4 metabolism, Ketotifen pharmacology, Liver metabolism, Male, Rats, Rats, Wistar, Tumor Necrosis Factor-alpha metabolism, Hepatitis metabolism, Hepatitis therapy, Histamine Antagonists pharmacology, Liver drug effects, Th1-Th2 Balance
- Abstract
Objective: To investigate the effects of antihistamine treatment on immune function in rats with experimental hepatitis., Methods: Thirty Wistar rats were randomly allocated into three groups:experimental hepatitis group (EH group), antihistamine treatment group (AH group) and normal control group (NC group). Rats in the EH group received the subcutaneous injection of 40% carbon tetrachloride oil solution and were fed on diet with low-protein, low-choline, high-fat and high-alcohol,while rats in the AH group received antihistamine treatment(ketotifen + vitamin C) additionally.They were sacrificed after 4 weeks, and the levels of serum alanine aminotransferase(ALT), total bilirubin (TBil), histamine(HA), IFNgamma, IL-12, IL-4 and IL-10 were determined. The levels of IL-12 mRNA and IFN-gamma mRNA in liver tissue were determined via real-time reverse transcriptional polymerase chain reaction(RT-PCR)., Results: (1) Compared to the NC group, in the EH group, the levels of ALT, TBil, and circulating and intrahepatic HA were significantly increased(P less than 0.05); intrahepatic HA were significantly decreased(P less than 0.05) after antihistamine treatment. (2) Compared to the NC group, in the EH group, the levels of IL-4, IL-10 were significantly increased((0.504+/-0.202)ng/ml and (29.025+/-1.478) pg/ml vs (0.811+/-0.244)ng/ml and (33.72+/-4.293)pg/ml respectively, P less than 0.05), and the levels of IL-12 were decreased ((6.515+/-2.893)pg/ml vs (3.519+/-1.113)pg/ml, P less than 0.05); and after antihistamine treatment the levels of IL-4 and IL-10 were significantly decreased (were (0.423+/-0.168)ng/ml and (30.412+/-3.275)pg/ml, P less than 0.05), the levels of IL-12 were significantly increased (P less than 0.05), but the level of IFNgamma had no significance (P more than 0.05). The levels of intrahepatic IL-12 mRNA and IFNgamma mRNA had similar results., Conclusion: Antihistamine treatment may improve liver function and correct Th1/Th2 unbalance.
- Published
- 2013
- Full Text
- View/download PDF
6. [Effects of lipopolysaccharide on the maturation and secretion of human peripheral dendritic cells].
- Author
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Li H, Zhao LF, Hao YQ, and Han DW
- Subjects
- Cells, Cultured, Dendritic Cells cytology, Humans, Lipopolysaccharides pharmacology, Monocytes cytology, Dendritic Cells drug effects, Dendritic Cells metabolism, Interleukin-12 biosynthesis, Monocytes metabolism
- Abstract
Objective: To study the effects of Lipopolysaccharide (LPS) on the maturation and secretion of human peripheral dendritic cells (DCs)., Methods: DCs from healthy human peripheral monocytes (PBMCs) were induced in vitro with rhGM-CSF, rhIL-4, Flt3-L and TNFalpha. The subjects were divided into 3 groups: the long-term group stimulated with LPS 1 microg/ml at day 1, 4, 7, 9 post culture; the short-term group stimulated with LPS 1 microg/ml at day 7 and 8 post culture, and the DCs without LPS stimulation was control group. After 10 days of culture, the morphologic features of DCs were observed by light and electron microscopes, the phenotypic patterns were characterized by flow cytometry, the proliferation of T cell were evaluated with mixed leukocytes reaction (MLR) and the levels of IL-12 and IFNgamma produced by DCs were analyzed with ELISA., Results: Compared with the short-term group, the expressions of HLA-DR (65.81%+/-10.96%), CD86 (48.81%+/-18.13%), CD80 (13.56%+/-5.48%), CD83 (11.52%+/-5.09%), the secretions of IFNgamma(15.60+/-5.83 pg/ml) and IL-12 (51.77+/-11.02 pg/ml) by the DCs in long-term group were decreased obviously (P is less than 0.05) and the proliferation of homogenic lymphocyte cells (1.548+/-0.365) stimulated by DCs was also impaired (P < 0.05)., Conclusion: Long-term LPS stimulation can suppress the maturation and secretion of DCs, which might be the reason of poor immunity in the patients with intestinal endotoxemia.
- Published
- 2010
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7. [Isolation and in vitro culture of bone marrow-derived mesenchymal stem cells].
- Author
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Hao YQ, Lu N, Li H, and Zhao LF
- Subjects
- Animals, Cells, Cultured, Male, Rats, Rats, Wistar, Bone Marrow Cells cytology, Cell Separation methods, Mesenchymal Stem Cells cytology
- Published
- 2010
- Full Text
- View/download PDF
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