Search

Objective To investigate the expression of PRR11 in bladder cancer tissues and its effect on proliferation and apoptosis of bladder cancer cell line T24. Methods The expression of PRR11 was detected using immunohistochemistry method in 57 specimens of bladder urothelial carcinoma and adjacent tissues. The correlations of PRR11 expression with the clinicopathological characteristics of patients with bladder urothelial carcinoma were analyzed. The mRNA and protein expression levels of PRR11 in human immortalized bladder epithelial cell lines SV-HUC-1 and human bladder cancer cell lines HTB-9, T24, J82 and UM-UC-3 were measured by qRT-PCR and Western blot. The gene expression of PRR11 in T24 cells was silenced by lentivirus shRNA. The mRNA expression level of PRR11 was detected by qRT-PCR. CCK-8 was used to detect cell proliferative activity. Cell clonality was detected by plate cloning assays. The rate of apoptosis was evaluated using flow cytometry. The protein expression levels of PRR11, Caspase-3, Bcl-2 and Bax were assessed by Western blot. Results PRR11 was highly expressed in bladder urothelial carcinoma, and its expression level was correlated with the pathological grade and T stage of the tumor. The mRNA and protein expression levels of PRR11 in HTB-9, T24, J82 and UM-UC-3 cells were higher than those in SV-HUC-1 cells (P < 0.05), especially in T24 cells. PRR11 gene silence reduced the expression levels of PRR11 mRNA and protein, as well as the cell proliferation activity and cell clonality, elevated the apoptosis rate, up-regulated the protein expression levels of Cleaved caspase-3 and Bax, and down-regulated the protein expression level of Bcl-2. Conclusion The expression of PRR11 is upregulated in bladder urothelial carcinoma tissues and bladder cancer cell lines. Interfering with PRR11 expression can inhibit the proliferation and promote the apoptosis of bladder cancer T24 cells.

Parts of sections in permafrost regions of the Qinghai-Tibet Railway (QTR) have been deformed due to permafrost degradation, the global warming and human activities. Therefore, monitoring the subgrade settlement of the QTR in permafrost regions has been a major working in QTR maintenance. For this reason, this paper investigates the subsidence characteristics and derivative mechanisms of railway subgrades in the permafrost regions of the QTR. Additionally, it analyses the impact mechanisms of surface temperature changes on the subsidence of permafrost subgrades. Building on this investigation, a C-I-GWO-BP neural network prediction model was developed to forecast subgrade settlement in permafrost regions. The findings reveal a substantial correlation (R2 = 0.834) between the deformation of railway subgrades in permafrost regions and changes in surface temperature. Meanwhile，with the intensification of global warming and human activities, the subsidence of permafrost subgrade has shown an increasing trend year by year. The primary areas of deformation are predominantly concentrated in ice-rich permafrost regions, including rivers, lakes, and snowmelt runoff areas. Additionally, when compared to traditional BP neural network models, the C-I-GWO-BP neural network prediction model proposed in this article showcases diminished prediction errors and heightened accuracy in identifying optimal weights and thresholds. This model offers technical support for the efficient, accurate, and automated prediction of railway subgrade subsidence in permafrost regions. [ABSTRACT FROM AUTHOR]

The aim of the paper is to study a coupled system which consists of a fractional differential hemivariational inequality (FDHVI, for short) combined with a fractional hemivariational inequality (FHVI, for short). By utilizing Rothe method and surjectivity result, the existence of coupled system is established. In addition, we apply the results to a new quasistatic contact problem, in which the constitutive equations are described by the fractional Kelvin-Voigt laws. [ABSTRACT FROM AUTHOR]

The aim of the paper is to study a new class of coupled variational–hemivariational inequalities with unilateral constraints and history‐dependent operators. First, by employing a fixed‐point theorem for history‐dependent operators combined with surjectivity theorem, we establish the existence and uniqueness of solution for coupled system. Second, we obtain a convergence result, namely, related penalized problems of solutions converge to the solution to the original system. Finally, we conclude with a new contact problem of the body pressure of two elastic materials. [ABSTRACT FROM AUTHOR]