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Your search keyword '"Hao, Guizeng"' showing total 6 results
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6 results on '"Hao, Guizeng"'

2. Discovery of potential transcriptional biomarkers in broiler chicken for detection of amantadine abuse based on RNA sequencing technology

Author

Zhang Kunpeng, Hao Guizeng, Li Qiong, You Xinyong, Xu Huaide, and Meijuan Xu

Subjects
Genetic Markers, animal structures, Transcription, Genetic, Health, Toxicology and Mutagenesis, Biology, Real-Time Polymerase Chain Reaction, Toxicology, 01 natural sciences, Genome, 03 medical and health sciences, Immune system, Gene expression, Amantadine, medicine, Animals, Receptor, Gene, 030304 developmental biology, Principal Component Analysis, 0303 health sciences, Sequence Analysis, RNA, 010401 analytical chemistry, Public Health, Environmental and Occupational Health, RNA, General Chemistry, General Medicine, Metabolism, 0104 chemical sciences, Biochemistry, Chickens, Food Science, medicine.drug
Abstract

The aim of this study was to identify candidate transcriptional biomarkers so as to provide a new method for monitoring amantadine residues during the feeding of broiler chicken. RNA sequencing (RNA-seq) and bioinformatic analyses were conducted to examine the transcriptomic changes and screen differentially expressed genes (DEGs) in broiler chicken breast muscle and liver tissues treated with amantadine. The results indicated that a total of 170 DEGs were screened from broiler chicken breast muscle tissues after amantadine was fed. Among the genes, 120 were up-regulated and 50 were down-regulated. The gene ontology (GO) terms for these genes mainly existed in the areas of hydrolase activity, immune reaction and chemokine activity. The significantly enriched pathways in the Kyoto Encyclopedia for Genes and Genomes (KEGG) were in phagosomes, cell adhesion molecules (CAMs), lysosomes and extracellular matrix (ECM) receptors. From the broiler chicken liver tissues, 172 DEGs were screened, among which 116 were up-regulated and 56 were down-regulated. The GO terms of these DEGs were related to functions such as catalytic activities, metabolic activities, oxidation-reduction activities, immune reactions and cofactor binding. The significantly enriched KEGG pathways existed in metabolism, CAM, ECM receptor reaction and drug metabolism-cytochrome P450. According to the fold-change (FC), significance levels, functional annotations and possible biological processes of DEGs, 11 and 9 candidate DEGs related to amantadine treatment were further screened from broiler chicken breast muscle and liver tissues, respectively. In addition, the quantitative real-time polymerase chain reaction (qRT-PCR) verification showed exactly concordant results with the RNA-seq data. Principal components analysis (PCA) on the qRT-PCR data resulted in the separation of treated samples from the control samples in both tissues. The results provided a basis for identification of transcriptional biomarkers for detecting amantadine residues in broiler chicken breast muscle and liver tissues.

Published
2019

6. La0.7Sr0.3FeO3− α perovskite hollow fiber membranes for oxygen permeation and methane conversion

Author

Tan, Xiaoyao, Shi, Lili, Hao, Guizeng, Meng, Bo, and Liu, Shaomin

Subjects
*LANTHANUM compounds, *PEROVSKITE, *HOLLOW fibers, *OXYGEN, *METHANE, *SINTERING, *CERAMIC powders
Abstract

Abstract: Gas-tight La0.7Sr0.3FeO3− α (LSF) perovskite hollow fiber membranes were fabricated by the phase inversion/sintering technique using self-made ceramic powder. The permeation and reaction properties of the LSF membrane were investigated systematically by using He, CO2, H2 and CH4 as the sweep or reactive gas, respectively. The experimental results indicate that the cobalt-free LSF hollow fiber membrane exhibits good stability when exposed in inert gases like He and weak acid gases like CO2 but is not stable in a reducing environment. Continuous and severe segregation may occur when the LSF membrane is operated in H2 and CH4 atmosphere. The oxygen permeation rate is highly dependent on the formation rate of oxygen vacancies on the membrane sweeping side surface. Compared to He as the sweep gas, the use of CO2 restrained the oxygen permeation due to the strong chemical adsorption on the membrane surface. However, the LSF membrane still suffers from the reaction with H2 and CH4 to form porous debris on the membrane surface, which may initially promote the oxygen flux but will finally result in membrane leaking or even breakage. When using H2 or CH4 as the sweep gas, the oxygen in the air feed can be depleted completely by permeating through the membrane. Although the LSF membrane exhibits a kind of catalytic activity to the partial oxidation of methane into syngas with a maximum CO yield of 1.2%, additional catalyst is required to avoid the deep oxidation of the products so that high syngas yields can be achieved. [Copyright &y& Elsevier]

Published
2012
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