31 results on '"Hanudin, Hanudin"'
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2. Synergistic Action of PGPR and Biofungicide with Active Ingredient of Cladosporium clasporioides to Control White Rust on Chrysanthemum
- Author
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Wakiah Nuryani, Hanudin Hanudin, Indijarto Budi Rahardjo, and Kurniawan Budiarto
- Subjects
antagonist microbes ,chrysanthemum ,consortium ,white rust ,Agriculture ,Plant culture ,SB1-1110 - Abstract
The application of antagonist microbes to control significant diseases on crops is an essential issue in the eco-friendly and sustainable agriculture of the chrysanthemum production system. The application of antagonist consortiums is expected to synergistically suppress the pathogen more effectively than a single microbe, thus increasing the marketable flower yield. The research is carried out to evaluate the single and combined application of antagonists C. ladosporioides and PGPRs, B. subtilis, and P. flourescens to control white rust in Chrysanthemum. The results show that there is no cumulative effect from the combination of biofungicide and the PGPR on disease suppression, disease incidence, and plant growth improvement than single antagonist treatments. Compared to synthetic fungicide, biofungicide and the PGPR treatments give higher parasitism intensity, though the values were negligible among the treatments. The lowest disease intensity is recorded from synthetic fungicide treatment. The improvement of flower quality due to biofungicide, PGPR, and synthetic fungicide treatments was found only on the longer vase life than untreated plants. Observation on the compatibility of antagonists with PGPRs are still needed to increase the effectiveness in controlling white rust in Chrysanthemum.
- Published
- 2021
- Full Text
- View/download PDF
3. Konservasi in vitro dua aksesi lili mealui modifikasi media kultur
- Author
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Kurniawan Budiarto, Indijarto Budi Raharjo, Hanudin Hanudin, and Wakiah Nuryani
- Subjects
keragaan in vivo, konsentrasi sukrosa, konservasi in vitro, lili, modifikasi media ,Agriculture (General) ,S1-972 - Abstract
Konservasi in vitro merupakan salah satu alternatif penyimpanan materi genetik dalam kondisi aseptik. Metode ini dapat mengurangi resiko kepunahan materi genetik akibat kondisi lingkungan yang ekstrem dan kerumitan pengelolaan pada skala in vivo terutama untuk tanaman yang berasal dari subtropis, seperti lili. Metode penghambatan pertumbuhan dan ketahanan plantlet selama perlakuan konservasi merupakan faktor penting untuk kelangsungan hidup materi genetik yang disimpan. Penelitian bertujuan untuk mengevaluasi media konservasi melalui modifikasi kandungan nutrien dan konsentrasi sukrosa untuk konservasi in vitro jangka menengah terhadap dua aksesi lili yaitu lokal Lilium longiflorum dan cv. Candilongi. Penelitian dilakukan di Balai Penelitian Tanaman Hias mulai Januari 2015 sampai September 2017. Hasil penelitian menunjukkan laju kematian plantlet semakin meningkat seiring dengan lamanya waktu penyimpanan. Laju kematian plantlet dengan pertumbuhan daun yang terendah diperlihatkan oleh plantlet Lili yang dikonservasi pada media ¼ MS + 7 % sukrosa dengan kisaran 27-31 % setelah 24 bulan penyimpanan. Dua genotipe lili menunjukkan respon pertumbuhan yang berbeda selama konservasi in vitro dan in vivo, namun tidak pada media konservasi yang dicoba. Plantlet lili lokal L. longiflorum mempunyai jumlah daun lebih sedikit selama konservasi in vitro. Sedangkan cv. Candilongi memperlihatkan tinggi tanaman yang lebih rendah dengan jumlah bunga lebih banyak dan ukuran bunga lebih besar pada evaluasi in vivo. ABSTRACT In vitro conservation is an alternative method of plant genetic resources preservation in aseptic condition. The method has successfully applied in many crops and reduced the limitation of in vivoconservation of non-native crop as Lily planted in the tropic area. Growth inhibition method and plantlet resistance are important factors to ensure the life-span of the conserved plantlet during in vitro storage. The research aimed to evaluate the media compositions and sucrose concentrations for mid-term conservation of two Lilium accessions, local L. longiflorum and cv. Candilongi. The experiment was carried out at Indonesian Ornamental Crops Research Institute from January 2015 to September 2017. The results showed the plantlet death rates increased in line with the duration of storages. After 24 months storage, plantlets conserved under ¼ MS + 7% sucrose showed fewer numbers of leaves and death plantlets in the range of 27-31%. Growth plantlet retardation during storage was merely detected on local L. longiflorum that showed less leaves development. Plantlet of cv. Candilongi had shorter plant height, yet bigger flower size and higher number of flower per plant under in vivo evaluation.
- Published
- 2020
- Full Text
- View/download PDF
4. Aplikasi dan efektivitas pupuk hayati dalam upaya perbaikan mutu produksi, produktivitas dan pengendalian serangan layu fusarium pada bawang merah
- Author
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Wakiah Nuryani, Hanudin Hanudin, and Kurniawan Budiarto
- Subjects
allium ascalonicum l., bobot umbi, efektifitas, fusarium oxysporum f. sp. cepae (snyder and hans.), pupuk hayati ,Agriculture (General) ,S1-972 - Abstract
Bawang merah (Allium ascalonicum L.) merupakan salah satu komoditas sayuran unggulan nasional yang mempunyai nilai ekonomi tinggi. Untuk pencapai target produksi yang maksimal, umumnya petani menggunakan pupuk dan pestisida kimia sintetik yang tinggi. Penurunan daya dukung lahan pertanian akibat penggunaan bahan kimia agroinput berlebihan mendorong penggunaan pupuk hayati berbahan aktif mikroba pemicu pertumbuhan terutama untuk mengurangi penggunaan pupuk kimia sintetik pada bawang merah. Penelitian bertujuan untuk mengevaluasi beberapa pupuk hayati yang dikombinasikan dengan pupuk kimia sintetik untuk meningkatkan kualitas pertumbuhan dan produksi bawang merah, serta menekan penyakit layu fusarium. Penelitian menggunakan Rancangan Acak Kelompok dengan 3 ulangan. Perlakuan berupa tiga jenis pupuk hayati, yaitu Agrofit, Bio Pf dan Biotrico dikombinasikan dengan dosis pupuk kimia sintetik yaitu sebesar 0, 25, 50, 75, 100% dari dosis rekomendasi. Hasil penelitian menunjukkan bahwa aplikasi kombinasi pupuk hayati Agrofit, Bio-Pf dan Biotrico dengan 50% dosis rekomendasi pupuk kimia sintetik dapat meningkatkan kualitas pertumbuhan dan bobot umbi kering sebesar 7.91 – 32.65% dari aplikasi pupuk kimia sintetik 100% dosis rekomendasi. Pada kombinasi Bio-Pf yang ditambah dengan 25% pupuk kimia sintetik dosis rekomendasi menunjukkan nilai efektifitas relatif agronomi dan nilai penekanan terhadap infeksi fusarium tertinggi. Kombinasi perlakuan ini juga memberikan total biomasa tidak berbeda pada dosis pupuk kimia sintetik 100%. ABSTRACT Shallot is one of important vegetables in Indonesia. Expecting high yield, farmers tend to apply high amount of chemical fertilizer and shynthetic pesticide. The concern to the negative impacts of chemical agroinput in agriculture production has induced the application of biofertilizer containing beneficial microbes to reduce the use of synthetic fertilizers in shallot production. The research was conducted to evaluate several formulated biofertilizers combined with synthetic fertilizers on growth, yield and productivity improvement, and fusarium wilt control on shallot. The research was conducted at Sukabumi, West Java, Indonesia from March to August 2017. The combination of bio-fertilizer types, i.e. Agrofit, Bio-Pf and Biotrico and synthetic fertilizers in different dosages, i.e 0, 25, 50, 75, 100 % from recommended dosages were arranged in completely randomized block design with three replications. The results showed the application of bio-fertilizers might reduce 50% of synthetic fertilizer usage. The combined application of each bio-fertilizer with 50% recommended dosage of synthetic fertilizers improved growth quality and total dry bulb weight of 7.91 – 32.65% from 100% recommended dosage of synthetic fertilizer. The combination of Bio-Pf and Agrofit with 25% recommended synthetic fertilizer gave highest relative agronomic effectiveness value and fusarium wilt suppression. Total biomass weight of these treatments also had negligible differences with 100% synthetic fertilizers dosages.
- Published
- 2020
- Full Text
- View/download PDF
5. Genetic Relationship Analysis of Chrysanthemum Genotypes Based on Quantitative and Qualitative Characters
- Author
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Sanjaya, Liauw Lia, primary, Hanudin, Hanudin, additional, Budiarto, Kurniawan Budiarto, additional, Mawaddah, Mawaddah, additional, Rahardjo, Indijarto Budi Rahardjo, additional, Fitri Rachmawati, Fitri Rachmawati, additional, Ridho, Kurniati, additional, Shintiavira, Herni, additional, Indrasti, Rita, additional, Rawung, Jefny B. Markus, additional, Djoko, Mulyono, additional, Suhendar, M. Ace, additional, Sajimin, Sajimin, additional, Abdul Ghaffar, Abdul Ghaffar, additional, R. Bambang Heryanto, R. Bambang Heryanto, additional, Widiarsih, Sasanti, additional, and Dwimahyani, Ita, additional
- Published
- 2023
- Full Text
- View/download PDF
6. Combined Application of Bio-PF and Synthetic Fungicide Suppress Soil Borne Disease Caused by Cylindrocladium sp. in Leather Leaf
- Author
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Hanudin Hanudin, Wakiah Nuryani, Evi Silvia Yusuf, and Kurniawan Budiarto
- Subjects
cut foliage ,cylindrocladium sp ,fungicide ,pseudomonas fluorescens ,ruhmora adiantiformis ,Agriculture ,Plant culture ,SB1-1110 - Abstract
An evaluation of formulated Bio-PF with the active ingredient of Pseudomonas fluorescens to reduce synthetic fungicide usage in controlling soil borne disease in leather leaf fern under different shelters was studied. The research was conducted at the experimental field of the Indonesian Ornamental Crops Research Institute (IOCRI) from January to December 2015. Different concentrations of Bio-PF were singly and/nor alternately applied with two different synthetic fungicides were applied on leather leaf plants under UV plastic + 70% black-net and 70% black-net shelters. The results showed that the average disease intensity was higher in 70% black-net shelter. Weekly alternate-application of Bio-PF 5 + Carbendazim & Mancozeb and single Carbendazim & Mancozeb gave lower disease intensity than other treatments with the highest percentage of suppression. These two treatments also improved yield in terms of number and proportion of the preferred grade of harvested leaves. Combined Bio-PF 5 + Carbendazim & Mancozeb gave longer vase life and the application reduced synthetic fungicide usage in controlling the respected disease. In term of synthetic fungicides, Carbendazim & Mancozeb gave more consistent effects on disease suppression, plant growth and foliage production than Asilbensolar & Mancozeb.
- Published
- 2019
- Full Text
- View/download PDF
7. Physico-Chemical, Viability Evaluations and Efficacy Assessment of Bacillus subtilis Against Soft Rot Disease in Phalaenopsis
- Author
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Wakiah Nuryani, Hanudin Hanudin, Evi Silvia Yusuf, and Kurniawan Budiarto
- Subjects
Bacillus subtilis ,Biological control ,Efficacy evaluation ,Phalaenopsis ,Soft rot ,Agriculture ,Plant culture ,SB1-1110 - Abstract
The study of biological agents in controlling plant disease has discovered many potential microbes with various mode of actions. In the end, these potential microorganism should qualifiedly fulfill several requirements before they are formally stated for commercialization and wider implementation. Evaluations on their physico-chemical characteristics and viabililty after certain storage period were needed to ensure the effectiveness of the product during transportation and commercialization process. The research was aimed to evaluate B. subtilis strains B7 and B30 for their physio-chemical characteristic and viability after six months storage and investigate the efficacy of the strains against soft root disease of Phalaenopsis. The results showed that both antagonists had stable perfomances in physico-chemical, viability and efficacy evaluations after six months storage. The existence of active bacteria was demonstrated from the decrease in pH, murky suspensions and foul smelling as indications of fermentative reactions under optimal and minimal nutrients. Both antagonists showed stable viability after storage and effectively inhibited soft rot disease when applied on the infected plants with slightly lesser supression from streptomycin sulphate.
- Published
- 2018
- Full Text
- View/download PDF
8. Evaluasi Resistensi Progeni Anggrek Phalaenopsis terhadap Penyakit Busuk Lunak (Pseudomonas viridiflava)
- Author
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Hanudin Hanudin, Indijarto Budi Rahardjo, and Kurniawan Budiarto
- Subjects
crossing progeny, orchid nursery, resistant response ,Botany ,QK1-989 - Abstract
A bacterial soft rot disease caused by P. viridiflava was one of problems in orchid nursery, especially Phalaenopsis. An evaluation on progenies derived from three crossing combinations were conducted to find out the symptom characteristic, development of infections of the disease and resistance response of the progenies against artificial infection of P. viridiflava. The research was conducted under a standard glass house conditions for Phalaenopsis involving 31 individuals from 3 progeny populations, namely P.157, E.2153 and E.2189. The results showed that symptoms were characterized by the lesion of the leaf tissues. On most accessions, the lesion enlarged and foul odor was detected as an indication of the successful establishment of bacterial infection. Based on the disease severity, three accessions (P.157-12, P.157-45, and P.157-71) had the lowest infections and were classified into resistant genotypes. One accession, i.e. P.157-32 was classified as susceptible and the rest 27 accessions were grouped into very susceptible class.
- Published
- 2018
- Full Text
- View/download PDF
9. Synergistic Action of PGPR and Biofungicide with Active Ingredient of Cladosporium clasporioides to Control White Rust on Chrysanthemum
- Author
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Nuryani, Wakiah, primary, Hanudin, Hanudin, additional, Rahardjo, Indijarto Budi, additional, and Budiarto, Kurniawan, additional
- Published
- 2022
- Full Text
- View/download PDF
10. Konservasi in vitro dua aksesi lili mealui modifikasi media kultur
- Author
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Wakiah Nuryani, Kurniawan Budiarto, Indijarto Budi Raharjo, and Hanudin Hanudin
- Subjects
keragaan in vivo, konsentrasi sukrosa, konservasi in vitro, lili, modifikasi media ,lcsh:Agriculture (General) ,lcsh:S1-972 - Abstract
Konservasi in vitro merupakan salah satu alternatif penyimpanan materi genetik dalam kondisi aseptik. Metode ini dapat mengurangi resiko kepunahan materi genetik akibat kondisi lingkungan yang ekstrem dan kerumitan pengelolaan pada skala in vivo terutama untuk tanaman yang berasal dari subtropis, seperti lili. Metode penghambatan pertumbuhan dan ketahanan plantlet selama perlakuan konservasi merupakan faktor penting untuk kelangsungan hidup materi genetik yang disimpan. Penelitian bertujuan untuk mengevaluasi media konservasi melalui modifikasi kandungan nutrien dan konsentrasi sukrosa untuk konservasi in vitro jangka menengah terhadap dua aksesi lili yaitu lokal Lilium longiflorum dan cv. Candilongi. Penelitian dilakukan di Balai Penelitian Tanaman Hias mulai Januari 2015 sampai September 2017. Hasil penelitian menunjukkan laju kematian plantlet semakin meningkat seiring dengan lamanya waktu penyimpanan. Laju kematian plantlet dengan pertumbuhan daun yang terendah diperlihatkan oleh plantlet Lili yang dikonservasi pada media ¼ MS + 7 % sukrosa dengan kisaran 27-31 % setelah 24 bulan penyimpanan. Dua genotipe lili menunjukkan respon pertumbuhan yang berbeda selama konservasi in vitro dan in vivo , namun tidak pada media konservasi yang dicoba. Plantlet lili lokal L. longiflorum mempunyai jumlah daun lebih sedikit selama konservasi in vitro . Sedangkan cv. Candilongi memperlihatkan tinggi tanaman yang lebih rendah dengan jumlah bunga lebih banyak dan ukuran bunga lebih besar pada evaluasi in vivo . ABSTRACT In vitro conservation is an alternative method of plant genetic resources preservation in aseptic condition. The method has successfully applied in many crops and reduced the limitation of in vivo conservation of non-native crop as Lily planted in the tropic area. Growth inhibition method and plantlet resistance are important factors to ensure the life-span of the conserved plantlet during in vitro storage. The research aimed to evaluate the media compositions and sucrose concentrations for mid-term conservation of two Lilium accessions, local L. longiflorum and cv. Candilongi. The experiment was carried out at Indonesian Ornamental Crops Research Institute from January 2015 to September 2017. The results showed the plantlet death rates increased in line with the duration of storages. After 24 months storage, plantlets conserved under ¼ MS + 7% sucrose showed fewer numbers of leaves and death plantlets in the range of 27-31%. Growth plantlet retardation during storage was merely detected on local L. longiflorum that showed less leaves development. Plantlet of cv. Candilongi had shorter plant height, yet bigger flower size and higher number of flower per plant under in vivo evaluation.
- Published
- 2020
11. Combined Application of Bio-PF and Synthetic Fungicide Suppress Soil Borne Disease Caused by Cylindrocladium sp. in Leather Leaf
- Author
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Wakiah Nuryani, Hanudin Hanudin, Evi Silvia Yusuf, and Kurniawan Budiarto
- Subjects
Acibenzolar ,biology ,Carbendazim ,Crop yield ,Vase life ,lcsh:S ,lcsh:Plant culture ,biology.organism_classification ,Fungicide ,lcsh:Agriculture ,chemistry.chemical_compound ,Horticulture ,ruhmora adiantiformis ,chemistry ,Ornamental plant ,fungicide ,Mancozeb ,cylindrocladium sp ,lcsh:SB1-1110 ,Cylindrocladium ,cut foliage ,pseudomonas fluorescens ,Agronomy and Crop Science - Abstract
An evaluation of formulated Bio-PF with the active ingredient of Pseudomonas fluorescens to reduce synthetic fungicide usage in controlling soil borne disease in leather leaf fern under different shelters was studied. The research was conducted at the experimental field of the Indonesian Ornamental Crops Research Institute (IOCRI) from January to December 2015. Different concentrations of Bio-PF were singly and/nor alternately applied with two different synthetic fungicides were applied on leather leaf plants under UV plastic + 70% black-net and 70% black-net shelters. The results showed that the average disease intensity was higher in 70% black-net shelter. Weekly alternate-application of Bio-PF 5 + Carbendazim & Mancozeb and single Carbendazim & Mancozeb gave lower disease intensity than other treatments with the highest percentage of suppression. These two treatments also improved yield in terms of number and proportion of the preferred grade of harvested leaves. Combined Bio-PF 5 + Carbendazim & Mancozeb gave longer vase life and the application reduced synthetic fungicide usage in controlling the respected disease. In term of synthetic fungicides, Carbendazim & Mancozeb gave more consistent effects on disease suppression, plant growth and foliage production than Asilbensolar & Mancozeb.
- Published
- 2019
12. Identification of Ralstonia solanacearum Isolated from a New Host : Cosmos caudatus in Indonesia
- Author
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Hanudin Hanudin, Kurniawan Budiarto, and Budi Marwoto
- Subjects
Biology (General) ,QH301-705.5 ,Ecology ,QH540-549.5 - Abstract
The evolutionary dynamic of pathogenic microorganism might change pathogen-host interaction including the establishment of pathogen colonization in a new host. The information of the new host of devastating pathogens will serve a basis for their control method. An unknown bacterial wilt disease attacking wild cosmos (Cosmos caudatus) crop in Pacet-Cianjur, Indonesia was suspiciously caused a novel strain of Ralstonia solanacearum. The research was carried out to investigate the causal agent, characteristics and its grouping based on the morphological, physiological and biochemical properties and the relationship of the pathogen with the hosts. The results showed the incidence of bacterial wilt on wild cosmos was epidemiologically caused by the species complex of pathogen R. solanacearum. Based on the symptoms, morphological, physiological, biochemical and pathogenicity evaluations, the strain classified into group 7, race 1 and biovar 3 and group 7. This was the first report of the incidence of R. solanacearum hosted naturally in wild cosmos in Indonesia.
- Published
- 2014
- Full Text
- View/download PDF
13. Bacterial Leaf Blight Disease (Pseudomonas cichorii (Swingle 1925) (STAPP 1928) in Chrysanthemum (Dendranthema grandiflora Tzvelev) and Its Control) in Indonesia
- Author
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Hanudin, Hanudin, primary, Sanjaya, Lia, additional, and Marwoto, Budi, additional
- Published
- 2020
- Full Text
- View/download PDF
14. Aplikasi dan efektivitas pupuk hayati dalam upaya perbaikan mutu produksi, produktivitas dan pengendalian serangan layu fusarium pada bawang merah
- Author
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Nuryani, Wakiah, primary, Hanudin, Hanudin, additional, and Budiarto, Kurniawan, additional
- Published
- 2020
- Full Text
- View/download PDF
15. Konservasi in vitro dua aksesi lili mealui modifikasi media kultur
- Author
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Budiarto, Kurniawan, primary, Raharjo, Indijarto Budi, additional, Hanudin, Hanudin, additional, and Nuryani, Wakiah, additional
- Published
- 2020
- Full Text
- View/download PDF
16. Bacterial Leaf Blight Disease (Pseudomonas cichorii (Swingle 1925) (STAPP 1928) in Chrysanthemum (Dendranthema grandiflora Tzvelev) and Its Control) in Indonesia
- Author
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Lia Sanjaya, Hanudin Hanudin, and Budi Marwoto
- Subjects
Horticulture ,biology ,Dendranthema grandiflora ,Blight ,biology.organism_classification ,Pseudomonas cichorii - Abstract
Bacterial leaf blight caused by Pseudomonas cichorii is a major disease in chrysanthemum plants almost all over the world. In Indonesia, this pathogen can cause damage to chrysanthemum 10- 60%. Bacteria spread from one plant to another through water droplets from modern irrigation networks as well as conventional irrigation. P. cichorii is a polyphagic pathogen, which infects succulents and others across continents with varying incidence. Symptoms of transmission of this pathogen in each type of plant are always different, and effective control methods have not been found. This article discusses the virulence of pathogens, the incidence of transmission, and recommendations for controlling bacterial leaf blight on chrysanthemums in Indonesia. A search of various references from within and outside the country shows that P. cichorii can be controlled by combining several methods, namely (a) the use of tolerant varieties (Puspita Nusantara, Puspa Kania, Dwina Kencana, Dwina Pelangi, Pasopati, Paras Ratu, and Wastu Kania), (b) technical culture (extracting infected leaves and watering in the morning), and (c) application of synthetic chemical bactericides with active ingredients of hydrogen peroxide and peroxyacetic acid, or biopesticides with active bacterial isolates of the antagonistic bacteria Bacillus subtilis MI600, and B. amyloliquefaciens IN937, and combination of P. fluorescens Pf Irana with Pf Slada-2.Keywords: Chrysanthemum, P. chicorii, bacterial leaf blight disease, epidemiology, control AbstrakPENYAKIT HAWAR DAUN BAKTERI (Pseudomonas cichorii (Swingle 1925) (STAPP 1928) PADA TANAMAN KRISAN (Dendranthema grandiflora Tzvelev) DAN UPAYA PENGENDALIANNYA DI INDONESIAHawar daun bakteri yang disebabkan oleh Pseudomonas cichorii merupakan penyakit utama pada tanaman krisan hampir di seluruh penjuru dunia. Di Indonesia, patogen ini dapat menyebabkan kerusakan pada tanaman krisan 10-60%. Bakteri menyebar dari satu tanaman ke tanaman lain melalui tetesan air dari jaringan irigasi modern maupun penyiraman konvensional. P. cichorii merupakan patogen yang bersifat polifag, yang menginfeksi tanaman sukulen dan lainnya di seluruh benua dengan insidensi bervariasi. Gejala penularan patogen ini pada setiap jenis tanaman selalu berbeda, dan belum ditemukan metode pengendalian yang efektif. Artikel ini membahas virulenitas patogen, insidensi penularan, dan rekomendasi pengendalian hawar daun bakteri pada tanaman krisan di Indonesia. Penelusuran dari berbagai referensi dari dalam dan luar negeri menunjukkan P. cichorii dapat dikendalikan dengan memadukan beberapa metode, yaitu (a) penggunaan varietas toleran (Puspita Nusantara, Puspa Kania, Dwina Kencana, Dwina Pelangi, Pasopati, Paras Ratu, dan Wastu Kania), (b) kultur teknis (perompesan daun terinfeksi dan penyiraman pada pagi hari), serta (c) aplikasi bakterisida kimia sintetik berbahan aktif hydrogen peroxide dan peroxyacetic acid, atau biopestisida berbahan aktif isolat bakteri antagonis Bacillus subtilis MI600, dan B. amyloliquefaciens IN937, serta kombinasi P. fluorescens Pf Irana dengan Pf Slada-2.Kata kunci: Krisan, P. chicorii, bakteri hawar daun, epidemiologi, pengendalian.
- Published
- 2020
17. Physico-Chemical, Viability Evaluations and Efficacy Assessment of Bacillus subtilis Against Soft Rot Disease in Phalaenopsis
- Author
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Kurniawan Budiarto, Wakiah Nuryani, Hanudin Hanudin, and Evi Silvia Yusuf
- Subjects
Phalaenopsis ,business.industry ,Microorganism ,lcsh:S ,Root disease ,Bacillus subtilis ,lcsh:Plant culture ,Biology ,Efficacy evaluation ,biology.organism_classification ,Soft rot ,Soft rot disease ,Plant disease ,Biotechnology ,lcsh:Agriculture ,Biological control ,Streptomycin sulphate ,lcsh:SB1-1110 ,business ,Agronomy and Crop Science ,Bacteria - Abstract
The study of biological agents in controlling plant disease has discovered many potential microbes with various mode of actions. In the end, these potential microorganism should qualifiedly fulfill several requirements before they are formally stated for commercialization and wider implementation. Evaluations on their physico-chemical characteristics and viabililty after certain storage period were needed to ensure the effectiveness of the product during transportation and commercialization process. The research was aimed to evaluate B. subtilis strains B7 and B30 for their physio-chemical characteristic and viability after six months storage and investigate the efficacy of the strains against soft root disease of Phalaenopsis. The results showed that both antagonists had stable perfomances in physico-chemical, viability and efficacy evaluations after six months storage. The existence of active bacteria was demonstrated from the decrease in pH, murky suspensions and foul smelling as indications of fermentative reactions under optimal and minimal nutrients. Both antagonists showed stable viability after storage and effectively inhibited soft rot disease when applied on the infected plants with slightly lesser supression from streptomycin sulphate.
- Published
- 2018
18. POTENSI BEBERAPA MIKROBA PEMACU PERTUMBUHAN TANAMAN SEBAGAI BAHAN AKTIF PUPUK DAN PESTISIDA HAYATI
- Author
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Hanudin, Hanudin, primary, Budiarto, Kurniawan, additional, and Marwoto, Budi, additional
- Published
- 2018
- Full Text
- View/download PDF
19. Application of PGPR and Antagonist Fungi-based Biofungicide for White Rust Disease Control and Its Economyc Analysis in Chrysanthemum Production
- Author
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Kurniawan Budiarto, Budi Marwoto, and Hanudin Hanudin
- Subjects
0301 basic medicine ,Production cost ,food and beverages ,Biology ,Rhizobacteria ,Rust ,Disease control ,Fungicide ,Toxicology ,03 medical and health sciences ,Biopesticide ,biology.plant_disease ,030104 developmental biology ,Agronomy ,Production (economics) ,Puccinia horiana ,Agronomy and Crop Science - Abstract
Plant growth promoting rhizobacteria (PGPR) application in combination with other antagonist microbes as biopesticide have been considered in many crops. Our research was conducted to evaluate the efficacy of these useful combinations with the carrying agent for growth promotion, thus lowering white rust incidence in chrysanthemum production. The experiment was carried out at three cooperative farmer sites located in Cipanas, Cianjur, West Java, Indonesia from January to December 2016. The production process was arranged in a paired treatment; a combination of PGPR and antagonist fungi (without supplemental chemical fertilizers and fungicide), furtherly called biofungicide and common farmer practices. The results showed that the application of biofungicide promoted equal plant growth quality as common practices. White rust incidency was lower at biofungicide treatment sites, thus increased the markertable flowers quantity. The production cost was considered more efficient in biofungicide sites, due to cheaper price of biofungicide than chemical fertilizers and fungicide. The increase of marketable stalks and cost efficiency led to an increase of net income of biofungicide-based production as also viewed from higher Revenue Cost Ratio (R/C) than common farmer practices.
- Published
- 2017
20. Physico-Chemical, Viability Evaluations and Efficacy Assessment of Bacillus subtilis Against Soft Rot Disease in Phalaenopsis
- Author
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Nuryani, Wakiah, primary, Hanudin, Hanudin, additional, Yusuf, Evi Silvia, additional, and Budiarto, Kurniawan, additional
- Published
- 2018
- Full Text
- View/download PDF
21. Aplikasi Biofungisida Berbahan Aktif Corynebacterium sp. Ramah Lingkungan dalam Pengendalian Penyakit Karat Putih pada Krisan
- Author
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Nuryani, Wakiah, primary, Silvia, Evi, additional, Hanudin, Hanudin, additional, and Budiarto, Kurniawan, additional
- Published
- 2018
- Full Text
- View/download PDF
22. Aplikasi Biofungisida Berbahan Aktif Corynebacterium sp. Ramah Lingkungan dalam Pengendalian Penyakit Karat Putih pada Krisan
- Author
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Evi Silvia, Wakiah Nuryani, Hanudin Hanudin, Kurniawan Budiarto, and IOCRI
- Subjects
General Medicine - Abstract
Kepedulian terhadap kelestarian lingkungan dan dampak negatif dari intensifikasi kegiatan budidaya dan penggunaan bahan kimia berbahaya dalam proses produksi tanaman merupakan isu strategis yang berkembang saat ini. Penggunaan agroinput kimiawi seperti pestisida dan pupuk sintetik memiliki dampak negatif terhadap lingkungan sekitar, termasuk kualitas ekosistem dan kesehatan manusia, juga terhadap penurunan daya dukung lingkungan. Penggunaan beberapa agensia hayati sebagai pengendali organisme pengganggu tanaman diharapkan mampu mengurangi bahkan mensubstitusi total penggunaan bahan kimia sintetis. Penggunaan biofungisida berbahan aktif Corynebacterium yang diformulasi dengan penambahan bakteri perakaran pemicu pertumbuhan tanaman (PGPR) ditujukan tidak hanya untuk mengendalikan penyakit karat sekaligus meningkatkan pertumbuhan dan hasil bunga pada krisan sebagai elemen budidaya yang ramah lingkungan. Penelitian bertujuan untuk mendapatkan komposisi dan teknik aplikasi biofungisida berbahan aktif Corynebacterium sp. yang diformulasi dengan PGPR terbaik dalam mengendalikan penyakit karat putih serta dapat meningkatkan hasil panen bunga krisan layak jual. Penelitian dilaksanakan dari bulan Januari hingga Desember 2014 di dalam laboratorium dan rumah plastik Balithi dengan menggunakan RAK dengan 4 ulangan. Hasil terbaik yang diperoleh kemudian diaplikasikan di lahan petani untuk verifikasi lebih lanjut. Hasil penelitian menunjukkan bahwa perlakuan biofungisida berbahan aktif Corynebacterium sp. yang diformulasikan dengan PGPR 0.3% yang diaplikasikan melalui perendaman akar dan diikuti dengan penyemprotan dengan interval 7 hari memberikan penekanan terhadap penyakit sebesar 3.55%, perbaikan kualitas pertumbuhan dan persentase peningkatan bunga layak jual sebesar 4.92% dibandingkan dengan aplikasi pestisida sintetik yang umum digunakan petani.
- Published
- 2018
- Full Text
- View/download PDF
23. Application of PGPR and Antagonist Fungi-based Biofungicide for White Rust Disease Control and Its Economyc Analysis in Chrysanthemum Production
- Author
-
Hanudin, Hanudin, primary, Budiarto, Kurniawan, additional, and Marwoto, Budi, additional
- Published
- 2017
- Full Text
- View/download PDF
24. Identification of Ralstonia solanacearum Isolated from a New Host : Cosmos caudatus in Indonesia
- Author
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Kurniawan Budiarto, Hanudin Hanudin, and Budi Marwoto
- Subjects
Ralstonia solanacearum ,Ecology ,Host (biology) ,Bacterial wilt ,Biovar ,food and beverages ,Cosmos caudatus ,Biology ,biology.organism_classification ,Microbiology ,Cosmos (plant) ,Colonization ,General Agricultural and Biological Sciences ,Pathogen ,Biotechnology - Abstract
Normal 0 false false false MicrosoftInternetExplorer4 The evolutionary dynamic of pathogenic microorganism might change pathogen-host interaction including the establishment of pathogen colonization in a new host. The information of the new host of devastating pathogens will serve a basis for their control method. An unknown bacterial wilt disease attacking wild cosmos ( Cosmos caudatus ) crop in Pacet-Cianjur, Indonesia was suspiciously caused a novel strain of Ralstonia solanacearum . The research was carried out to investigate the causal agent, characteristics and its grouping based on the morphological, physiological and biochemical properties and the relationship of the pathogen with the hosts. The results showed the incidence of bacterial wilt on wild cosmos was epidemiologically caused by the species complex of pathogen R. solanacearum . Based on the symptoms, morphological, physiological, biochemical and pathogenicity evaluations, the strain classified into group 7, race 1 and biovar 3 and group 7. This was the first report of the incidence of R. solanacearum hosted naturally in wild cosmos in Indonesia. st1\:*{behavior:url(#ieooui) } /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;}
- Published
- 2014
25. Kemangkusan Kandidat Biofungisida Berbahan Aktif Bakteri Antagonis terhadap Fusarium oxysporum pada Phalaenopsis
- Author
-
Djatnika, I, primary, Hanudin, Hanudin, additional, and Nuryani, Wakiah, additional
- Published
- 2016
- Full Text
- View/download PDF
26. Komposisi Formula Biobakterisida Berbahan Aktif Rizobakteri untuk Pengendalian Penyakit Busuk Lunak Pada Anggrek Phalaenopsis
- Author
-
Hanudin, Hanudin, primary, Nawangsih, Abdjad Asih, additional, Marwoto, Budi, additional, and Tjahjono, Boedi, additional
- Published
- 2016
- Full Text
- View/download PDF
27. Kemangkusan Kandidat Biofungisida Berbahan Aktif Bakteri Antagonis terhadap Fusarium oxysporum pada Phalaenopsis
- Author
-
Hanudin Hanudin, Wakiah Nuryani, and Ika Djatnika
- Subjects
Horticulture ,biology ,Fusarium oxysporum ,Phalaenopsis ,biology.organism_classification - Abstract
Layu fusarium yang disebabkan oleh Fusarium oxysporum pada tanaman anggrek dapat menimbulkan kerusakan tanaman hingga mencapai 40%. Bakteri antagonis B 37 dan B 26 hasil isolasi dari tanaman anggrek efektif mengendalikan penyakit layu fusarium pada tanaman anggrek masing-masing sebesar 65,3 dan 48,9%. Isolat bakteri tersebut belum diidentifikasi, diuji kompatibilitasnya, dan diaplikasikan masih dalam bentuk biakan murni. Tujuan penelitian ini ialah: (a) mengidentifikasi secara biokimia isolat bakteri B 26 dan B 37, (b) mendapatkan formulasi bahan pembawa organik yang kompatibel dengan isolat bakteri B 26 dan atau B 37, serta (c) mengendalikan F. oxysporum pada tanaman anggrek Phalaenopsis dengan menggunakan kedua bakteri tersebut yang sudah dikemas dalam bahan pembawa. Hasil penelitian menunjukkan bahwa (a) hasil identifikasi secara biokimia bakteri B 26 dan B 37 ialah Bacillus spp., (b) semua bahan aktif calon biopestisida (Bacillus spp. B 26 dan B 37) kompatibel terhadap bahan pembawa dari bahan alami yang mengandung karbohidrat dan protein minimal, tetapi kurang kompatibel pada media pembawa dari bahan alami yang mengandung karbohidrat dan protein optimal serta air steril, dan (c) perlakuan Bacillus spp. B26 atau B 37 dalam media pembawa yang mengandung karbohidrat dan protein minimal atau disuspensikan dalam air (disuspensikan dalam air setiap akan dilakukan perlakuan) yang diaplikasikan dengan merendam benih selama 1 jam yang kemudian diikuti dengan penyemprotan tanaman setiap 7 hari, efektif mengendalikan penyakit layu fusarium pada anggrek Phalaenopsis.
- Published
- 2016
28. Komposisi Formula Biobakterisida Berbahan Aktif Rizobakteri untuk Pengendalian Penyakit Busuk Lunak Pada Anggrek Phalaenopsis
- Author
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Boedi Tjahjono, Abdjad Asih Nawangsih, Budi Marwoto, and Hanudin Hanudin
- Subjects
Physics ,Horticulture ,biology ,Pseudomonas viridiflava ,Pseudomonas fluorescens ,Phalaenopsis ,biology.organism_classification - Abstract
Penyakit busuk lunak (PBL) yang disebabkan oleh Pectobacterium carotovorum subsp. carotovorum atau Pseudomonas viridiflava merupakan kendala utama dalam budidaya anggrek. Serangan patogen tersebut sangat merugikan petani, mengingat biaya investasi produksi anggrek tergolong tinggi. Oleh karena itu patogen tersebut harus dikendalikan menggunakan metode pengendalian yang ramah lingkungan, yaitu dengan mengaplikasikan biobakterisida berbahan aktif rizobakteri, seperti Bacillus subtilis dan Pseudomonas fluorescens. Tujuan penelitian ini ialah (1) mendapatkan komposisi bahan aktif dan bahan pembawa biobakterisida yang efektif mengendalikan penyakit busuk lunak pada anggrek Phalaenopsis, (2) mengetahui perubahan reaksi kimia formula biobakterisida dan pertumbuhan populasi bahan aktif (rizobakteri B. subtilis dan P. fluorescens) pada kondisi sebelum dan setelah difermentasikan, dan (3) mengetahui kompatibilitas antara B. subtilis, P. fluorescens, dan bahan pembawa biobakterisida. Percobaan dilaksanakan mulai Bulan Mei sampai dengan Desember 2009 di Laboratorium Bakteriologi Departemen Proteksi Tanaman, Institut Pertanian Bogor, dan Laboratorium Bakteriologi serta Rumah Kaca, Balai Penelitian Tanaman Hias, di Segunung, Cianjur, Jawa Barat. Ruang lingkup penelitian meliputi pembuatan propagul rizobakteri sebagai bahan aktif biobakterisida, pembuatan formula biobakterisida, uji viabilitas bahan aktif, dan uji kemangkusan biobakterisida pada tanaman anggrek di rumah kaca. Hasil penelitian menunjukkan bahwa (1) perlakuan gabungan antara B. subtilis B12 dan P. fluorescens Pf10 yang difermentasikan dalam media ekstrak kotoran cacing (kascing) dan molase, merupakan perlakuan yang konsisten dapat menekan PBL pada anggrek Phalaenopsis dengan persentase penekanan sebesar 80%, (2) reaksi kimia formula biopestisida pada kondisi sebelum dan setelah fermentasi diindikasikan dengan perubahan pH basal medium yang sebelum fermentasi menunjukkan pH 3,75 dan berubah menjadi pH 3,50 setelah difermentasikan. Pertumbuhan populasi mikrob antagonis setelah fermentasi meningkat secara signifikan bila dibandingkan pada kondisi sebelum difermentasikan, dan (3) isolat bahan aktif (B. subtilis dan P. fluorescens) bersifat kompatibel dengan bahan pembawanya (ekstrak kascing dan molase).
- Published
- 2016
29. Penapisan Beberapa Isolat Pseudomonas fluorescens, Bacillus subtilis dan Trichoderma harzianum yang bersifat Antagonistik terhadap Ralstonia solanacearum pada Tanaman Kentang
- Author
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Oni Setiani Gunawan, Andang Purnama, Hanudin Hanudin, Hersanti Hersanti, Budi Marwoto, and Rian Triyanti Rupendi
- Subjects
Microbiology - Abstract
Penyakit layu bakteri yang disebabkan oleh R. solanacearum merupakan salah satu penyakit penting yang menyerang tanaman kentang. Salah satu alternatif pengendalian yang ramah lingkungan adalah menggunakan mikroba antagonis seperti Pseudomonas fluorescens , Bacillus subtilis, dan Trichoderma harzianum. Tujuan penelitian adalah untuk mendapatkan isolat mikroba antagonis terbaik yang paling menekan penyakit layu bakteri pada tanaman kentang. Dua percobaan pada penelitian ini menggunakan Rancangan Acak Lengkap. Percobaan in vitro menguji daya hambat tiga isolat P. Fluorescens , dua isolat B. subtilis , satu isolat B. cereus dan 3 isolat T. harzianum terhadap perkembangan koloni R. solanacearum pada media King’s B. Percobaan in vivo menguji kemampuan seluruh isolat terhadap penekanan serangan penyakit layu pada tanaman kentang. Hasil percobaan menunjukkan bahwa isolat T. harzianum 1, T. harzianum 2 dan T. harzianum 3 mampu menekan penyakit layu bakteri pada tanaman kentang dengan persentase penghambatan berturut-turut 77,21 %, 77,21 %, dan 63,57 %.
- Published
- 2009
30. KARAKTERISTIK PSEUDOMONAS VIRIDIFLAVA: PENYEBAB PENYAKIT BUSUK LUNAK DAN EVALUASI VIRULENSINYA PADA KLON ANGGREK PHALAENOPSIS
- Author
-
Hanudin, Hanudin, primary and Rahardjo, Indijarto Budi, additional
- Published
- 2011
- Full Text
- View/download PDF
31. Penapisan Beberapa Isolat Pseudomonas fluorescens, Bacillus subtilis dan Trichoderma harzianum yang bersifat Antagonistik terhadap Ralstonia solanacearum pada Tanaman Kentang
- Author
-
Hersanti, Hersanti, primary, Rupendi, Rian Triyanti, additional, Purnama, Andang, additional, Hanudin, Hanudin, additional, Marwoto, Budi, additional, and Gunawan, Oni Setiani, additional
- Published
- 2009
- Full Text
- View/download PDF
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