18 results on '"Hanss, Zoé"'
Search Results
2. Generation of isogenic control DJ-1-delP GC13 for the genetic Parkinson‘s disease-patient derived iPSC line DJ-1-delP (LCSBi008-A-1)
- Author
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Mencke, Pauline, primary, Hanss, Zoé, additional, Jarazo, Javier, additional, Massart, François, additional, Rybicki, Arkadiusz, additional, Petkovski, Elizabet, additional, Glaab, Enrico, additional, Boussaad, Ibrahim, additional, Bonifati, Vincenzo, additional, Christian Schwamborn, Jens, additional, Mandemakers, Wim, additional, and Krüger, Rejko, additional
- Published
- 2022
- Full Text
- View/download PDF
3. Mitochondrial and Clearance Impairment in p.D620N VPS35 Patient-Derived Neurons
- Author
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Hanss, Zoé, Larsen, Simone, Antony, Paul, Mencke, Pauline, Massart, François, Jarazo, Javier, Schwamborn, Jens Christian, Barbuti, Peter, Mellick, George, Krüger, Rejko, Fonds National de la Recherche - FnR [sponsor], European Commission - EC [sponsor], Fondation du Pelican [sponsor], Luxembourg Centre for Systems Biomedicine (LCSB): Clinical & Experimental Neuroscience (Krüger Group) [research center], Luxembourg Centre for Systems Biomedicine (LCSB): Developmental and Cellular Biology (Schwamborn Group) [research center], and Griffith Institute for Drug Discovery [research center]
- Subjects
mitochondrial impairment ,induced pluripotent stem cells ,Parkinson's disease ,alpha-synuclein ,Genetics & genetic processes [F10] [Life sciences] ,Génétique & processus génétiques [F10] [Sciences du vivant] ,VPS35 - Abstract
Background: VPS35 is part of the retromer complex and is responsible for the trafficking and recycling of proteins implicated in autophagy and lysosomal degradation, but also takes part in the degradation of mitochondrial proteins via mitochondria-derived vesicles. The p.D620N mutation of VPS35 causes an autosomal-dominant form of Parkinson’s disease (PD), clinically representing typical PD. Objective: Most of the studies on p.D620N VPS35 were performed on human tumor cell lines, rodent models overexpressing mutant VPS35, or in patient-derived fibroblasts. Here, based on identified target proteins, we investigated the implication of mutant VPS35 in autophagy, lysosomal degradation, and mitochondrial function in induced pluripotent stem cell-derived neurons from a patient harboring the p.D620N mutation. Methods: We reprogrammed fibroblasts from a PD patient carrying the p.D620N mutation in the VPS35 gene and from two healthy donors in induced pluripotent stem cells. These were subsequently differentiated into neuronal precursor cells to finally generate midbrain dopaminergic neurons. Results: We observed a decreased autophagic flux and lysosomal mass associated with an accumulation of α-synuclein in patient-derived neurons compared to controls. Moreover, patient-derived neurons presented a mitochondrial dysfunction with decreased membrane potential, impaired mitochondrial respiration, and increased production of reactive oxygen species associated with a defect in mitochondrial quality control via mitophagy. Conclusion: We describe for the first time the impact of the p.D620N VPS35 mutation on autophago-lysosome pathway and mitochondrial function in stem cell-derived neurons from an affected p.D620N carrier and define neuronal phenotypes for future pharmacological interventions
- Published
- 2020
4. Bidirectional Relation Between Parkinson’s Disease and Glioblastoma Multiforme
- Author
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Mencke, Pauline, Hanss, Zoé, Boussaad, Ibrahim, Sugier, Pierre-Emmanuel, Elbaz, Alexis, and Krüger, Rejko
- Subjects
Neurologie [D14] [Sciences de la santé humaine] ,Neurology [D14] [Human health sciences] - Published
- 2020
5. Induced pluripotent stem cell line (LCSBi001-A) derived from a patient with Parkinson's disease carrying the p.D620N mutation in VPS35
- Author
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Fonds National de la Recherche - FnR [sponsor], H2020 [sponsor], Larsen, Simone, Hanss, Zoé, Cruciani, Gérald, Massart, François, Barbuti, Peter, Mellick, George, Krüger, Rejko, Fonds National de la Recherche - FnR [sponsor], H2020 [sponsor], Larsen, Simone, Hanss, Zoé, Cruciani, Gérald, Massart, François, Barbuti, Peter, Mellick, George, and Krüger, Rejko
- Abstract
Fibroblasts were obtained from a 76 year-old man diagnosed with Parkinson's disease (PD). The disease is caused by a heterozygous p.D620N mutation in VPS35. Induced pluripotent stem cells (iPSCs) were generated using the CytoTune™-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific). The presence of the c.1858G > A base exchange in exon 15 of VPS35 was confirmed by Sanger sequencing. The iPSCs are free of genomically integrated reprogramming genes, express pluripotency markers, display in vitro differentiation potential to the three germ layers and have karyotypic integrity. Our iPSC line will be useful for studying the impact of the p.D620N mutation in VPS35 in vitro.
- Published
- 2020
6. A patient-based model of RNA mis-splicing uncovers treatment targets in Parkinson's disease.
- Author
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Luxembourg Centre for Systems Biomedicine (LCSB): Clinical & Experimental Neuroscience (Krüger Group) [research center], Luxembourg Centre for Systems Biomedicine (LCSB): Bioinformatics Core (R. Schneider Group) [research center], Luxembourg Centre for Systems Biomedicine (LCSB): Developmental and Cellular Biology (Schwamborn Group) [research center], Luxembourg Centre for Systems Biomedicine (LCSB): Biomedical Data Science (Glaab Group) [research center], Luxembourg Centre for Contemporary and Digital History (C2DH) > Digital Research Infrastructure (DRI) [research center], Fonds National de la Recherche - FnR [sponsor], German Research Council [sponsor], EU Joint Program-Neurodegenerative Diseases [sponsor], European Union Horizon2020 research and innovation programme [sponsor], National Institutes of Health (NIH) [sponsor], Boussaad, Ibrahim, Obermaier, Carolin D., Hanss, Zoé, Bobbili, Dheeraj R., Bolognin, Silvia, Glaab, Enrico, Wołyńska, Katarzyna, Weisschuh, Nicole, De Conti, Laura, May, Caroline, Giesert, Florian, Grossmann, Dajana, Lambert, Annika, Kirchen, Susanne, Biryukov, Maria, Burbulla, Lena F., Massart, Francois, Bohler, Jill, Cruciani, Gérald, Schmid, Benjamin, Kurz-Drexler, Annerose, May, Patrick, Duga, Stefano, Klein, Christine, Schwamborn, Jens Christian, Marcus, Katrin, Woitalla, Dirk, Vogt Weisenhorn, Daniela M., Wurst, Wolfgang, Baralle, Marco, Krainc, Dimitri, Gasser, Thomas, Wissinger, Bernd, Krüger, Rejko, Luxembourg Centre for Systems Biomedicine (LCSB): Clinical & Experimental Neuroscience (Krüger Group) [research center], Luxembourg Centre for Systems Biomedicine (LCSB): Bioinformatics Core (R. Schneider Group) [research center], Luxembourg Centre for Systems Biomedicine (LCSB): Developmental and Cellular Biology (Schwamborn Group) [research center], Luxembourg Centre for Systems Biomedicine (LCSB): Biomedical Data Science (Glaab Group) [research center], Luxembourg Centre for Contemporary and Digital History (C2DH) > Digital Research Infrastructure (DRI) [research center], Fonds National de la Recherche - FnR [sponsor], German Research Council [sponsor], EU Joint Program-Neurodegenerative Diseases [sponsor], European Union Horizon2020 research and innovation programme [sponsor], National Institutes of Health (NIH) [sponsor], Boussaad, Ibrahim, Obermaier, Carolin D., Hanss, Zoé, Bobbili, Dheeraj R., Bolognin, Silvia, Glaab, Enrico, Wołyńska, Katarzyna, Weisschuh, Nicole, De Conti, Laura, May, Caroline, Giesert, Florian, Grossmann, Dajana, Lambert, Annika, Kirchen, Susanne, Biryukov, Maria, Burbulla, Lena F., Massart, Francois, Bohler, Jill, Cruciani, Gérald, Schmid, Benjamin, Kurz-Drexler, Annerose, May, Patrick, Duga, Stefano, Klein, Christine, Schwamborn, Jens Christian, Marcus, Katrin, Woitalla, Dirk, Vogt Weisenhorn, Daniela M., Wurst, Wolfgang, Baralle, Marco, Krainc, Dimitri, Gasser, Thomas, Wissinger, Bernd, and Krüger, Rejko
- Abstract
Parkinson's disease (PD) is a heterogeneous neurodegenerative disorder with monogenic forms representing prototypes of the underlying molecular pathology and reproducing to variable degrees the sporadic forms of the disease. Using a patient-based in vitro model of PARK7-linked PD, we identified a U1-dependent splicing defect causing a drastic reduction in DJ-1 protein and, consequently, mitochondrial dysfunction. Targeting defective exon skipping with genetically engineered U1-snRNA recovered DJ-1 protein expression in neuronal precursor cells and differentiated neurons. After prioritization of candidate drugs, we identified and validated a combinatorial treatment with the small-molecule compounds rectifier of aberrant splicing (RECTAS) and phenylbutyric acid, which restored DJ-1 protein and mitochondrial dysfunction in patient-derived fibroblasts as well as dopaminergic neuronal cell loss in mutant midbrain organoids. Our analysis of a large number of exomes revealed that U1 splice-site mutations were enriched in sporadic PD patients. Therefore, our study suggests an alternative strategy to restore cellular abnormalities in in vitro models of PD and provides a proof of concept for neuroprotection based on precision medicine strategies in PD.
- Published
- 2020
7. Mitochondria interaction networks show altered topological patterns in Parkinson’s disease
- Author
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Fonds National de la Recherche Luxembourg, Ministère de l’Enseignement Supérieur et de la Recherche (Luxembourg), European Cooperation in Science and Technology, University of Tübingen, Hertie Institute for Clinical Brain Research, DZNE, European Research Council, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Zanin, Massimiliano, Santos, Bruno F. R., Antony, Paul M. A., Berenguer-Escuder, Clara, Larsen, Simone B., Hanss, Zoé, Barbuti, Peter A., Baumuratov, Aidos S., Grossmann, Dajana, Capelle, Christophe M., Weber, Joseph, Balling, Rudi, Ollert, Markus, Krüger, Rejko, Diederich, Nico J., He, Feng Q., Fonds National de la Recherche Luxembourg, Ministère de l’Enseignement Supérieur et de la Recherche (Luxembourg), European Cooperation in Science and Technology, University of Tübingen, Hertie Institute for Clinical Brain Research, DZNE, European Research Council, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Zanin, Massimiliano, Santos, Bruno F. R., Antony, Paul M. A., Berenguer-Escuder, Clara, Larsen, Simone B., Hanss, Zoé, Barbuti, Peter A., Baumuratov, Aidos S., Grossmann, Dajana, Capelle, Christophe M., Weber, Joseph, Balling, Rudi, Ollert, Markus, Krüger, Rejko, Diederich, Nico J., and He, Feng Q.
- Abstract
Mitochondrial dysfunction is linked to pathogenesis of Parkinson’s disease (PD). However, individual mitochondria-based analyses do not show a uniform feature in PD patients. Since mitochondria interact with each other, we hypothesize that PD-related features might exist in topological patterns of mitochondria interaction networks (MINs). Here we show that MINs formed nonclassical scale-free supernetworks in colonic ganglia both from healthy controls and PD patients; however, altered network topological patterns were observed in PD patients. These patterns were highly correlated with PD clinical scores and a machine-learning approach based on the MIN features alone accurately distinguished between patients and controls with an area-under-curve value of 0.989. The MINs of midbrain dopaminergic neurons (mDANs) derived from several genetic PD patients also displayed specific changes. CRISPR/CAS9-based genome correction of alpha-synuclein point mutations reversed the changes in MINs of mDANs. Our organelle-interaction network analysis opens another critical dimension for a deeper characterization of various complex diseases with mitochondrial dysregulation.
- Published
- 2020
8. Mitochondrial and Clearance Impairment in p. D620N VPS35 Patient‐Derived Neurons
- Author
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Hanss, Zoé, primary, Larsen, Simone B., additional, Antony, Paul, additional, Mencke, Pauline, additional, Massart, François, additional, Jarazo, Javier, additional, Schwamborn, Jens C., additional, Barbuti, Peter A., additional, Mellick, George D., additional, and Krüger, Rejko, additional
- Published
- 2020
- Full Text
- View/download PDF
9. A patient-based model of RNA mis-splicing uncovers treatment targets in Parkinson’s disease
- Author
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Boussaad, Ibrahim, primary, Obermaier, Carolin D., additional, Hanss, Zoé, additional, Bobbili, Dheeraj R., additional, Bolognin, Silvia, additional, Glaab, Enrico, additional, Wołyńska, Katarzyna, additional, Weisschuh, Nicole, additional, De Conti, Laura, additional, May, Caroline, additional, Giesert, Florian, additional, Grossmann, Dajana, additional, Lambert, Annika, additional, Kirchen, Susanne, additional, Biryukov, Maria, additional, Burbulla, Lena F., additional, Massart, Francois, additional, Bohler, Jill, additional, Cruciani, Gérald, additional, Schmid, Benjamin, additional, Kurz-Drexler, Annerose, additional, May, Patrick, additional, Duga, Stefano, additional, Klein, Christine, additional, Schwamborn, Jens C., additional, Marcus, Katrin, additional, Woitalla, Dirk, additional, Vogt Weisenhorn, Daniela M., additional, Wurst, Wolfgang, additional, Baralle, Marco, additional, Krainc, Dimitri, additional, Gasser, Thomas, additional, Wissinger, Bernd, additional, and Krüger, Rejko, additional
- Published
- 2020
- Full Text
- View/download PDF
10. Bidirectional Relation Between Parkinson's Disease and Glioblastoma Multiforme
- Author
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Mencke, Pauline, primary, Hanss, Zoé, additional, Boussaad, Ibrahim, additional, Sugier, Pierre-Emmanuel, additional, Elbaz, Alexis, additional, and Krüger, Rejko, additional
- Published
- 2020
- Full Text
- View/download PDF
11. Induced pluripotent stem cell line (LCSBi001-A) derived from a patient with Parkinson's disease carrying the p.D620N mutation in VPS35
- Author
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Larsen, Simone B., primary, Hanss, Zoé, additional, Cruciani, Gérald, additional, Massart, François, additional, Barbuti, Peter A., additional, Mellick, George, additional, and Krüger, Rejko, additional
- Published
- 2020
- Full Text
- View/download PDF
12. Mitochondria-mitochondria interaction networks show altered topological patterns in Parkinson’s disease
- Author
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Zanin, Massimiliano, primary, Santos, Bruno F. R., additional, Antony, Paul M.A., additional, Berenguer-Escuder, Clara, additional, Larsen, Simone B., additional, Hanss, Zoé, additional, Barbuti, Peter, additional, Baumuratov, Aidos S., additional, Grossmann, Dajana, additional, Capelle, Christophe, additional, Weber, Joseph, additional, Balling, Rudi, additional, Ollert, Markus, additional, Krüger, Rejko, additional, Diederich, Nico J., additional, and He, Feng Q., additional
- Published
- 2020
- Full Text
- View/download PDF
13. Quality Control Strategy for CRISPR-Cas9-Based Gene Editing Complicated by a Pseudogene
- Author
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Hanss, Zoé, primary, Boussaad, Ibrahim, additional, Jarazo, Javier, additional, Schwamborn, Jens C., additional, and Krüger, Rejko, additional
- Published
- 2020
- Full Text
- View/download PDF
14. DISSECTING GENETIC EPISTASIS IN FAMILIAL PARKINSON’S DISEASE USING A DIGENIC PATIENT-DERIVED STEM CELL MODEL
- Author
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Hanss, Zoé, Fonds National de la Recherche - FnR [sponsor], Luxembourg Centre for Systems Biomedicine (LCSB): Clinical & Experimental Neuroscience (Krüger Group) [research center], and Krüger, Rejko [superviser]
- Subjects
Glucocerebrosidase ,iPSC ,Multidisciplinaire, généralités & autres [D99] [Sciences de la santé humaine] ,alpha-synuclein ,Genetics & genetic processes [F10] [Life sciences] ,CRISPR-Cas9 ,Biochemistry, biophysics & molecular biology [F05] [Life sciences] ,Biochimie, biophysique & biologie moléculaire [F05] [Sciences du vivant] ,Génétique & processus génétiques [F10] [Sciences du vivant] ,Multidisciplinary, general & others [D99] [Human health sciences] ,Parkin - Abstract
Parkinson’s disease (PD) is the second most common neurodegenerative disorder worldwide. 10% of PD patients present a familial form of the disease implicating genetic mutations. A variability in terms of disease expressivity, severity and penetrance can be observed among familial cases. The idea that the classical one-gene one-trait model may not catch the full picture of genetic contribution to PD pathophysiology is increasingly recognized. Therefore, a polygenic model where multiple genes would influence the disease risk and the phenotypic traits in PD should be investigated. Mutations in PRKN, encoding the E3 ubiquitin-protein ligase Parkin, cause young onset autosomal recessive forms of PD. A variability in terms of clinical presentation and neuropathology have been observed in PD patients carrying mutations in Parkin. On the other hand, mutations in GBA were recently recognized as the most common genetic risk factor for developing PD. The incomplete penetrance of the disease in patients with GBA mutations may implicate other genetic factors. Therefore, it can be hypothesized that the interactions between common PD genes like PRKN and GBA can contribute to the phenotypic heterogeneity observed in PD cases. To explore this hypothesis, we generated patient-derived cellular models from several PD patients carrying pathogenic mutations in either both PRKN and GBA (triallelic models) or in only one of them (bi- or monoallelic models). We developed a novel strategy to gene edit the N370S mutation in GBA via CRISPR-Cas9, without interference with its respective pseudogene, which allows for the dissection of the role of GBA in the context of a PRKN mutation on an isogenic background. We identified a specific α-synuclein homeostasis in the triallelic model. The genetic and pharmacological rescue of GBA in the triallelic model modified the observed α-synuclein phenotype, proving the contribution of GBA to the observed phenotype. We then investigated whether Parkin was contributing to the phenotype. The modulation of Parkin function in the context of a GBA mutation induced a modification of the α-synuclein homeostasis. We therefore concluded that both PRKN and GBA are influencing α-synuclein homeostasis in the triallelic model. Nevertheless, the phenotypic outcome of the co-occurrence of these mutations was not additive nor synergistic. We therefore suggest the existence of an epistatic interaction between mutant GCase and Parkin that would underlie the clinical heterogeneity observed in PD patients carrying these mutations.
- Published
- 2019
15. Quality Control Strategy for CRISPRCas9- based Gene Editing Complicated by a Pseudogene
- Author
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Hanss, Zoé, Boussaad, Ibrahim, Jarazo, Javier, Schwamborn, Jens Christian, and Krüger, Rejko
- Subjects
Biochemistry, biophysics & molecular biology [F05] [Life sciences] ,Biochimie, biophysique & biologie moléculaire [F05] [Sciences du vivant] - Published
- 2019
16. Mitochondrial and Clearance Impairment in p.D620N VPS35 Patient‐Derived Neurons.
- Author
-
Hanss, Zoé, Larsen, Simone B., Antony, Paul, Mencke, Pauline, Massart, François, Jarazo, Javier, Schwamborn, Jens C., Barbuti, Peter A., Mellick, George D., and Krüger, Rejko
- Abstract
Background: VPS35 is part of the retromer complex and is responsible for the trafficking and recycling of proteins implicated in autophagy and lysosomal degradation, but also takes part in the degradation of mitochondrial proteins via mitochondria‐derived vesicles. The p.D620N mutation of VPS35 causes an autosomal‐dominant form of Parkinson's disease (PD), clinically representing typical PD. Objective: Most of the studies on p.D620N VPS35 were performed on human tumor cell lines, rodent models overexpressing mutant VPS35, or in patient‐derived fibroblasts. Here, based on identified target proteins, we investigated the implication of mutant VPS35 in autophagy, lysosomal degradation, and mitochondrial function in induced pluripotent stem cell‐derived neurons from a patient harboring the p.D620N mutation. Methods: We reprogrammed fibroblasts from a PD patient carrying the p.D620N mutation in the VPS35 gene and from two healthy donors in induced pluripotent stem cells. These were subsequently differentiated into neuronal precursor cells to finally generate midbrain dopaminergic neurons. Results: We observed a decreased autophagic flux and lysosomal mass associated with an accumulation of α‐synuclein in patient‐derived neurons compared to controls. Moreover, patient‐derived neurons presented a mitochondrial dysfunction with decreased membrane potential, impaired mitochondrial respiration, and increased production of reactive oxygen species associated with a defect in mitochondrial quality control via mitophagy. Conclusion: We describe for the first time the impact of the p.D620N VPS35 mutation on autophago‐lysosome pathway and mitochondrial function in stem cell‐derived neurons from an affected p.D620N carrier and define neuronal phenotypes for future pharmacological interventions. © 2020 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
17. The genetic architecture of mitochondrial dysfunction in Parkinson's Disease
- Author
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Luxembourg Centre for Systems Biomedicine (LCSB) [research center], Fonds National de la Recherche - FnR [sponsor], Krüger, Rejko, Larsen, Simone, Hanss, Zoé, Luxembourg Centre for Systems Biomedicine (LCSB) [research center], Fonds National de la Recherche - FnR [sponsor], Krüger, Rejko, Larsen, Simone, and Hanss, Zoé
- Abstract
Mitochondrial impairment is a well-established pathological pathway implicated in Parkinson’s disease (PD). Defects of the complex I of the mitochondrial respiratory chain have been found in post mortem brains from sporadic PD patients. Furthermore, several disease-related genes are linked to mitochondrial pathways, such as PRKN, PINK1, DJ-1 and HTRA2 and are associated to mitochondrial impairment. This phenotype can be caused by the dysfunction of mitochondrial quality control machinery at different levels: molecular, organellar or cellular. Mitochondrial unfolded protein response represents the molecular level and implicates various chaperones and proteases. If the molecular level of quality control is not sufficient, the organellar level is required and involves mitophagy and mitochondrial derived vesicles to sequester whole dysfunctional organelle or parts of it. Only when the impairment is too severe, it leads to cell death via apoptosis which defines the cellular level of quality control. Here we review how currently known PD-linked genetic variants interfere with the different levels of mitochondrial quality control. We discuss the graded risk concept of the most recently identified PARK loci (PARK 17-23) and some susceptibility variants such as GBA, LRRK2 and SNCA. Finally, the emerging concept of rare genetic variants as candidates for PD, such as HSPA9, TRAP1 and RHOT1 complete the picture of the complex genetic architecture of PD that will direct future precision medicine approaches.
- Published
- 2018
- Full Text
- View/download PDF
18. Plasma Amyloid Is Associated with White Matter and Subcortical Alterations and Is Modulated by Age and Seasonal Rhythms in Mouse Lemur Primates
- Author
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Gary, Charlotte, primary, Hérard, Anne-Sophie, additional, Hanss, Zoé, additional, and Dhenain, Marc, additional
- Published
- 2018
- Full Text
- View/download PDF
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